Exosomal Cargo in Ovarian Cancer Dissemination
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThe authors have submitted a manuscript entitled "Exosomal Cargo in Ovarian Cancer Dissemination." They have discussed the role of exosomes in OC and their potential for early-stage OC diagnosis. Minor corrections are required before further publishing.
The authors have summarized all biomarkers related to OC. If we want more significance, therapeutic and immune response related to EV and OC will be a plus.
1. Exosomes are predominantly enriched in the hypoxic tumor microenvironment, and ovarian tumors are naturally hypoxic. The specific impact of this characteristic on the roles of exosomes in OC should be discussed.
2. The EM images have very low resolution, similar to other images. Please provide high-resolution images.
Comments on the Quality of English LanguageModerate editing of English language required
Author Response
Dear Reviewer!
Here is our response to your comments in the form of the attached file
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for Authors
Reviewer’s report
MS: cimb-2680377
This review article by Dzhugashvili E. and Tamkovich S. provided an overview of exosome biology and compiled information about the molecular cargoes of exosomes in the context of ovarian cancer. The sections 1 and 2 disseminated general information about exosomes, the section 3 focused on exosomal molecular compositions in general and in ovarian cancer, and the section 4 gave a conclusion.
I may be wrong but I found that this review was just the list of evidence but lacked in-depth analysis and comparison of current evidence in order to synthesize the useful information and perspectives. This review requires an extensive revision, especially on the section 3, and added a new section to provide the authors’ perspectives on this topic.
Please also find below a few additional comments which I believe would help improve the quality of the manuscript.
- Page 5, lines 189-198 & Table 1: How did the authors decide the most specific exosomal proteins? Please be informed that the frequently identified proteins in the exosome isolates are not necessarily the most specific exosome markers. I would like to refer to the ISEV guidelines 2014 and 2018 regarding the optimal way to address exosomal proteins.
- I noticed that from the sub-sections 3.1 to 3.3, the references were quite often cited at the end of the paragraph. One may find the pattern of “one paragraph-one paper” is not different from the complied abstracts.
- The authors mentioned that “The concentration of exosomes in plasma and ascites is much higher than tumor cells (~108 versus 10) …”. What is the concentration unit of those miRNAs?
- Did Figure 2 need any copyright permission from the original source?
Comments on the Quality of English LanguageModerate editing of English language required
Author Response
Dear Reviewer!
Here is our response to your comments in the form of the attached file
Author Response File: Author Response.pdf
Reviewer 3 Report
Comments and Suggestions for AuthorsThe review provides a good overview of OC exosomal cargo. However there are some things missing.
lines 141-142. why is this sentence separated from the rest of the text?
Lines 147: This is heavily isolation method dependent. Thus the number provided is not correct. Please review:
https://doi.org/10.1016/j.aca.2019.09.022
Monolithic immunoaffinity chromatography can isolate exosomes in a concentration of 1.17 ± 0.41*10^12 from 250 µL of plasma, which is much higher than the number that is presented in the article.
In figure 1 you have specified that exosomes carry cytocines and metabolites, however, you have not been talking about them enough or at all in your review. These should be included since the title of your review is Exosomal Cargo in Ovarian Cancer Dissemination. There are for example also amino acids and sugars carried by the exosomes:
https://doi.org/10.1021/acs.analchem.0c01986
Lines 176-188: This section needs to be expanded. Please review the following study about the lipidome of exosomes:
https://doi.org/10.1016/j.ab.2022.114672
Why is this not reference in text: The reference to Figure 2 is: 402 Dzhugashvili E.I., Yunusova N.V., Yalovaya A.I., Grigorieva A.E., Sereda E.E., Kolomiets L.A., 403 Tamkovich S.N. Comparative assessment of the exosomal tumor-associated microRNA levels in 404 blood plasma and ascitic fluid in ovarian cancer patients. Advances in Molecular Oncology. 405 2023;10(2):108-116. (In Russ.) https://doi.org/10.17650/2313-805X-2023-10-2-108-116
Author Response
Dear Reviewer!
Here is our response to your comments in the form of the attached file
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
Comments and Suggestions for AuthorsThe authors did not intend to carefully consider my comments. While a few points were addressed, several suggestions that may improve the quality of the manuscript (if addressed) were avoided. In its current form, I could not consider support the publication of this manuscript
Here is the list of recent reviews on the roles of exosome cargo and ovarian cancer: PMID: 31405096, 36852533, 37007117, 37006229, 29844681
Comments on the Quality of English LanguageMinor editing of English language required.
Author Response
Dear Reviewer 2,
Please see attached file.
Sincerely yours,
Svetlana Tamkovich
Author Response File: Author Response.pdf
Reviewer 3 Report
Comments and Suggestions for AuthorsThe authors have sufficiently addressed the reviewers’ comments.
Author Response
Dear Reviewer 3,
On behalf of myself and of co-author, I express my deep gratitude to you for the high appreciation of our manuscript titled “Exosomal cargo in ovarian cancer dissemination”, authored by Ekaterina Dzhugashvili and Svetlana Tamkovich.
Sincerely yours,
Corresponding author
Svetlana Tamkovich
Author Response File: Author Response.pdf
