Hidden Pitfalls of Using Onion Pollen in Molecular Research

Round 1

Reviewer 1 Report

General Comments: The topic is interesting and valuable regarding the gene editing and manipulation in the field of molecular biology. However authors need to well-structured the manuscript. For that the manuscript needs to improve mostly the materials and methods section by explaining clearly the necessity and impact of wet pollination in this context. The images (Figure 2 and 4) are not clear enough to detection of pollenkitt. The images are in very low magnification and resolution that does not visibly distinguish the exine and intine. Improve the image quality that clearly focus on pollenkitt and visualize distinctly.    

 

Line No                                               Remarks

15                                                        Arrange alphabetically

46-47                                                   Is there any significance to writing in italicized form?

112                                                      "x" is not used traditionally. Use "."

170                                                      Written as stereo-microscope

189                                                      Follow the earlier comment of line no – 112

Author Response

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Author Response File: Author Response.pdf

Reviewer 2 Report

Review Report

Manuscript ID: cimb-2183504

The manuscript entitled “Hidden Pitfalls of Using Onion Pollen in Molecular Research” submitted to CIMB needs corrections and improvements. Generally, the manuscript is fine but still there are some minor comments for the improvement of the manuscript.

Abstract: In the abstract part please mention the specific objective of your study

Introduction:  In the introduction part please mention the history, importance and background of onion pollen and their use in molecular research

Materials and Methods:  Line 64 to 69 please mentions the date of planting, flowering and maturity of onion. Please also mention the site of the experiment. Line 71 mention the manufacturer name regarding analytical balance and line 70 to 92 reference missing regarding DNA extraction and PCR Analysis.

Carefully check all spellings, numerical order of references, format following the requirements of the submitted journals.

Cross check all the references cited in the text with reference list

Author Response

See the attached file

Author Response File: Author Response.pdf

Reviewer 3 Report

Please, see an enclosed file.

Comments for author File: Comments.pdf

Author Response

See the attached file

Author Response File: Author Response.pdf