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Volume 45
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10.3390/cimb45060286
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Communication

Characterization of Mammary Tumors Arising from MMTV-PyVT Transgenic Mice

by
Chien-Liang Liu
1,
Wen-Chien Huang
1,
Shih-Ping Cheng
1,2,
Ming-Jen Chen
1,2,
Chi-Hsin Lin
3,4,
Shao-Chiang Chang
3 and
Yuan-Ching Chang
1,*
1
Department of Surgery, MacKay Memorial Hospital and Mackay Medical College, Taipei 104217, Taiwan
2
Institute of Biomedical Sciences, Mackay Medical College, New Taipei City 252005, Taiwan
3
Department of Medical Research, MacKay Memorial Hospital, Taipei 104217, Taiwan
4
Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan City 320314, Taiwan
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2023, 45(6), 4518-4528; https://doi.org/10.3390/cimb45060286
Submission received: 6 May 2023 / Revised: 20 May 2023 / Accepted: 22 May 2023 / Published: 24 May 2023
(This article belongs to the Topic Animal Models of Human Disease)
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Abstract

Among genetically engineered mouse models of breast cancer, MMTV-PyVT is a mouse strain in which the oncogenic polyoma virus middle T antigen is driven by the mouse mammary tumor virus promoter. The aim of the present study was to perform morphologic and genetic analyses of mammary tumors arising from MMTV-PyVT mice. To this end, mammary tumors were obtained at 6, 9, 12, and 16 weeks of age for histology and whole-mount analyses. We conducted whole-exome sequencing to identify constitutional and tumor-specific mutations, and genetic variants were identified using the GRCm38/mm10 mouse reference genome. Using hematoxylin and eosin analysis and whole-mount carmine alum staining, we demonstrated the progressive proliferation and invasion of mammary tumors. Frameshift insertions/deletions (indels) were noted in the Muc4. Mammary tumors showed small indels and nonsynonymous single-nucleotide variants but no somatic structural alterations or copy number variations. In summary, we validated MMTV-PyVT transgenic mice as a multistage model for mammary carcinoma development and progression. Our characterization may be used as a reference for guidance in future research.
Keywords: breast cancer; MMTV-PyVT mice; whole-exome sequencing breast cancer; MMTV-PyVT mice; whole-exome sequencing

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MDPI and ACS Style

Liu, C.-L.; Huang, W.-C.; Cheng, S.-P.; Chen, M.-J.; Lin, C.-H.; Chang, S.-C.; Chang, Y.-C. Characterization of Mammary Tumors Arising from MMTV-PyVT Transgenic Mice. Curr. Issues Mol. Biol. 2023, 45, 4518-4528. https://doi.org/10.3390/cimb45060286

AMA Style

Liu C-L, Huang W-C, Cheng S-P, Chen M-J, Lin C-H, Chang S-C, Chang Y-C. Characterization of Mammary Tumors Arising from MMTV-PyVT Transgenic Mice. Current Issues in Molecular Biology. 2023; 45(6):4518-4528. https://doi.org/10.3390/cimb45060286

Chicago/Turabian Style

Liu, Chien-Liang, Wen-Chien Huang, Shih-Ping Cheng, Ming-Jen Chen, Chi-Hsin Lin, Shao-Chiang Chang, and Yuan-Ching Chang. 2023. "Characterization of Mammary Tumors Arising from MMTV-PyVT Transgenic Mice" Current Issues in Molecular Biology 45, no. 6: 4518-4528. https://doi.org/10.3390/cimb45060286

APA Style

Liu, C.-L., Huang, W.-C., Cheng, S.-P., Chen, M.-J., Lin, C.-H., Chang, S.-C., & Chang, Y.-C. (2023). Characterization of Mammary Tumors Arising from MMTV-PyVT Transgenic Mice. Current Issues in Molecular Biology, 45(6), 4518-4528. https://doi.org/10.3390/cimb45060286

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