Metabolic Deregulation in Pulmonary Hypertension
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Hemodynamic Data
2.3. Estimation of Protein Expression
2.4. Double Immunofluorescence
2.5. Statistical Analysis
2.6. Gene Expression Data
2.7. Transcriptomic Analyses
2.7.1. Significant Regulation
2.7.2. Measures of Individual Genes’ Contributions to the Transcriptomic Alteration
3. Results
3.1. Significant Increase of the RVSP and RV/LV Weight Ratio in All Three PAH Models
3.2. Pulmonary Arterial Hypertension Increased the Expression of the Nerve Growth Factor (Ngf), Nuclear Factor Erythroid-Derived 2-Like 2 (Nfe2l2), and Glucose Transporter Solute Carrier Family 2 (Slc2a1)
3.3. Independent Transcriptomic Characteristics
3.4. Measures of Transcriptomic Alterations
3.5. Regulation of Glycolysis/Gluconeogenesis
3.6. Regulation of the Citrate (TCA) Cycle
3.7. Remodeling of the Metabolic Genes Network
3.8. Regulation of Key Genes That Control Mitochondrial Membrane Potential and May Be Responsible for PAH Channelopathies
4. Discussion
5. Conclusions and Future Directions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Mathew, R.; Iacobas, S.; Huang, J.; Iacobas, D.A. Metabolic Deregulation in Pulmonary Hypertension. Curr. Issues Mol. Biol. 2023, 45, 4850-4874. https://doi.org/10.3390/cimb45060309
Mathew R, Iacobas S, Huang J, Iacobas DA. Metabolic Deregulation in Pulmonary Hypertension. Current Issues in Molecular Biology. 2023; 45(6):4850-4874. https://doi.org/10.3390/cimb45060309
Chicago/Turabian StyleMathew, Rajamma, Sanda Iacobas, Jing Huang, and Dumitru Andrei Iacobas. 2023. "Metabolic Deregulation in Pulmonary Hypertension" Current Issues in Molecular Biology 45, no. 6: 4850-4874. https://doi.org/10.3390/cimb45060309