Effects of Blood-Derived Products on Cellular Senescence and Inflammatory Response: A Study on Skin Rejuvenation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines and Media
2.2. Preparation of Blood Products
2.3. Growth Curves
2.4. Procedure for SA-ß-gal Staining
2.5. Immuno Fluorescence
2.6. Western Blot Analysis
2.7. XTT Assay
2.8. BP-Treatment for Early Treatemnt Group
2.8.1. Cell Seeding
2.8.2. Etoposide Treatment
2.8.3. BP-Treatment
2.8.4. Establishment of Cellular Senescence
2.8.5. Harvesting of Cells
2.9. BP-Treatment for Late Exposure Group
2.9.1. Seeding of Cells and Etoposide Treatment
2.9.2. Establishment of Cellular Senescence
2.9.3. BP-Treatment
2.9.4. Final Incubation and Harvest
2.10. RNA Extraction and qPCR
2.11. Luminex Assay
2.12. Statistics
3. Results
3.1. Definition of Reagents
Blood-Derived Products
3.2. Induction of Senescent State
3.3. Proliferation and Outgrowth of HDF Cells
3.4. Investigations in Cellular Senescence and SASP-Factors IL-6 and IL-8
3.4.1. p21 Expression
3.4.2. Type I Collagen Expression
3.4.3. IL-6 Concentration in Supernatant
3.4.4. IL-8 Concentration in Supernatant
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kühnel, H.; Pasztorek, M.; Kuten-Pella, O.; Kramer, K.; Bauer, C.; Lacza, Z.; Nehrer, S. Effects of Blood-Derived Products on Cellular Senescence and Inflammatory Response: A Study on Skin Rejuvenation. Curr. Issues Mol. Biol. 2024, 46, 1865-1885. https://doi.org/10.3390/cimb46030122
Kühnel H, Pasztorek M, Kuten-Pella O, Kramer K, Bauer C, Lacza Z, Nehrer S. Effects of Blood-Derived Products on Cellular Senescence and Inflammatory Response: A Study on Skin Rejuvenation. Current Issues in Molecular Biology. 2024; 46(3):1865-1885. https://doi.org/10.3390/cimb46030122
Chicago/Turabian StyleKühnel, Harald, Markus Pasztorek, Olga Kuten-Pella, Karina Kramer, Christoph Bauer, Zsombor Lacza, and Stefan Nehrer. 2024. "Effects of Blood-Derived Products on Cellular Senescence and Inflammatory Response: A Study on Skin Rejuvenation" Current Issues in Molecular Biology 46, no. 3: 1865-1885. https://doi.org/10.3390/cimb46030122