NMR Studies of the Interactions between Sialyllactoses and the Polysialytransferase Domain for Polysialylation Inhibition
Abstract
:1. Introduction
2. Methods and Materials
2.1. Material Sources
2.2. Preparations of Circular Dichroism (CD) Samples and the Measurements and Recorded Methods of CD Spectra
2.3. Preparation of NMR Samples
2.4. NMR Spectroscopic Measurements
3. Results
3.1. CD Data
3.2. NMR Results
3.3. The PSTD-(6′-SL) Interaction
3.4. Comparison of the Inhibition of the SLs and Heparin (LMWH) on Polysialylation
3.4.1. Comparison of the Inhibition of the SLs and Heparin (LMWH) on the PSTD-CMP-Sia Interaction
3.4.2. Comparison of the Inhibition of the SLs and Heparin (LMWH) on the PSTD-polySia Interaction
3.5. Comparison of the Inhibition of the SLs and CMP on Polysialylation
3.5.1. Comparison of the Inhibition of the 3′-SL and CMP on the PSTD-(CMP-Sia) Interaction
3.5.2. Comparison of the Inhibition of the 6′-SL and CMP on Polysialylation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
polySia | polysialic acid |
Sia | mono-sialic acid |
CMP-Sia | cytidine monophosphate-sialic acid |
NCAMs | neural cell adhesion molecules |
polySTs | polysialyltransferases (ST8Sia II (STX) & ST8Sia IV (PST) |
PSTD | polysialyltransferase domain |
SL | sialyllactose |
SMO | sialylated milk oligosaccharide |
HMO | human milk oligosaccharide |
CSP | chemical shift perturbation |
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The helix content of the PSTD alone (%): 26.5 | The helix content of the PSTD after CMP-Sia and polySia were added (%): 18.4 |
26.5 | The helix content of the PSTD after CMP-Sia, polySia and 3′-SL were added (%): 17.7 |
26.5 | The helix content of the PSTD after CMP-Sia, polySia and 3′-SL were added (%): 14.5 |
Sialylactose (SL) interacted with the PSTD | SL’s concentration when CSP values of PSTD-SL binding are close to that of PSTD-(CMP-Sia) binding in same amino acid range of the PSTD (mM) | SL’s concentration when CSP values of PSTD-SL binding are close to that of PSTD-polySia binding in same amino acid range of the PSTD (mM) | The maximum concentration of the SL in human milk during 1st month of lactation (mM) [9] |
3′-SL | 0.5 | 0.5 | Ca. 0.5 |
6′-SL | 1.0 | Ca. 3.0 | Ca. 1.0 |
Ligands Binding to the PSTD | The Main Binding Regions of the Ligands in the PSTD | The Maximum CSPs of Each Binding Region |
---|---|---|
CMP-Sia | K246–S257 V267–K276 | 0.070 0.026 |
polySia | A263–N271 | 0.049 |
Heparin LMWH | K246–S257 A263–R277 | 0.087 0.072 |
CMP | K246–S257 A263–R277 | 0.087 0.046 |
3′-SL (0.5 mM) | K246–S257 A263–R277 | 0.070 0.043 |
6′-SL (1 mM) | K246–S257 A263–R277 | 0.070 0.026 |
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Lu, B.; Liao, S.-M.; Liang, S.-J.; Li, J.-X.; Liu, X.-H.; Huang, R.-B.; Zhou, G.-P. NMR Studies of the Interactions between Sialyllactoses and the Polysialytransferase Domain for Polysialylation Inhibition. Curr. Issues Mol. Biol. 2024, 46, 5682-5700. https://doi.org/10.3390/cimb46060340
Lu B, Liao S-M, Liang S-J, Li J-X, Liu X-H, Huang R-B, Zhou G-P. NMR Studies of the Interactions between Sialyllactoses and the Polysialytransferase Domain for Polysialylation Inhibition. Current Issues in Molecular Biology. 2024; 46(6):5682-5700. https://doi.org/10.3390/cimb46060340
Chicago/Turabian StyleLu, Bo, Si-Ming Liao, Shi-Jie Liang, Jian-Xiu Li, Xue-Hui Liu, Ri-Bo Huang, and Guo-Ping Zhou. 2024. "NMR Studies of the Interactions between Sialyllactoses and the Polysialytransferase Domain for Polysialylation Inhibition" Current Issues in Molecular Biology 46, no. 6: 5682-5700. https://doi.org/10.3390/cimb46060340