Age-Related Effects of Inhalational Anesthetics in B4galnt1-Null and Cuprizone-Treated Mice: Clinically Relevant Insights into Demyelinating Diseases

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

It is necessary to discuss ganglioside findings in the context of Ranvier nodes.

Delete "and B4Galnt1-null (KO) mice" from Figure 7 title. (The expression level of four main brain gangliosides (GM1, GD1a, GD1b and GT1b) in corpus callosum of brains of wild-type (WT), cuprizone-treated wild-type 617 (WT+CPZ) and B4Galnt1-null (KO) mice.)

It is necessary to write limitation of lacking experimental group inhaled anesthetics to be compared to results without inhalation that are shown in Figure 7. 

Author Response

Thank you very much for taking the time to review this manuscript. Please find the detailed responses below and the corresponding revisions/corrections highlighted/in track changes in the re-submitted files.

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The manuscript written by Tot et al. entitled “Age-related effects of inhalational anesthetics in B4galnt1-null and cuprizone-treated mice: clinically relevant insight into demyelinating diseases” explores the effect of the inhalational anesthetics isofluorane and sevofluorane in an animal model of demyelination. The work is novel, well presented and interesting.

Comments:

Could the authors please explain why they used such a variable number of animals for each group?  One group consisted only of three animals, which is rather low.

Figures 5 and 7 quality should be improved, as they are a little blurry.

The conclusion is meandering, confusing, excessively long and hard to follow. The authors should focus on the results and on the direct comparison of the groups. Moreover, authors should focus on how these results could be extrapolated to the humans in a clinical setting.

Comments on the Quality of English Language

minor english editing required. 

Author Response

Thank you very much for taking the time to review this manuscript. Please find the detailed responses below and the corresponding revisions/corrections highlighted/in track changes in the re-submitted files.

Author Response File: Author Response.pdf