Green Tea Polyphenol (-)-Epicatechin Pretreatment Mitigates Hepatic Steatosis in an In Vitro MASLD Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. HepG2 Cell Culturing
2.2. Pretreatment with (-)-Epicatechin and Treatment with OA
2.3. MTS Assay
2.4. ELISA
2.5. Oil Red O Staining
Oil Red O Quantification
2.6. Measuring Intracellular GSH Levels
2.7. Statistical Analysis
3. Results
3.1. Establishment of an In Vitro Model of MASLD
3.2. EPI Pretreatment and Subsequent Oleic Acid Exposure in HepG2 Cells
Assessment of a 4 h EPI Pretreatment Alone on HepG2 Cell Metabolic Viability
3.3. Peroxisome Proliferator-Activated Receptors: PPARα and PPARγ
3.4. Visualization of Fatty Changes and ORO Quantification in HepG2 Cells
3.5. Intracellular GSH Concentration
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hefer, M.; Petrovic, A.; Roguljic, L.K.; Kolaric, T.O.; Kizivat, T.; Wu, C.H.; Tabll, A.A.; Smolic, R.; Vcev, A.; Smolic, M. Green Tea Polyphenol (-)-Epicatechin Pretreatment Mitigates Hepatic Steatosis in an In Vitro MASLD Model. Curr. Issues Mol. Biol. 2024, 46, 8981-8994. https://doi.org/10.3390/cimb46080531
Hefer M, Petrovic A, Roguljic LK, Kolaric TO, Kizivat T, Wu CH, Tabll AA, Smolic R, Vcev A, Smolic M. Green Tea Polyphenol (-)-Epicatechin Pretreatment Mitigates Hepatic Steatosis in an In Vitro MASLD Model. Current Issues in Molecular Biology. 2024; 46(8):8981-8994. https://doi.org/10.3390/cimb46080531
Chicago/Turabian StyleHefer, Marija, Ana Petrovic, Lucija Kuna Roguljic, Tea Omanovic Kolaric, Tomislav Kizivat, Catherine H. Wu, Ashraf A. Tabll, Robert Smolic, Aleksandar Vcev, and Martina Smolic. 2024. "Green Tea Polyphenol (-)-Epicatechin Pretreatment Mitigates Hepatic Steatosis in an In Vitro MASLD Model" Current Issues in Molecular Biology 46, no. 8: 8981-8994. https://doi.org/10.3390/cimb46080531