Efficacy of SGPP2 Modulation-Mediated Materials in Ameliorating Facial Wrinkles and Pore Sagging
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Preparation
2.2. Transfection of Keratinocytes with siRNA for RNAi Experiments
2.3. RNA Extraction and RT-qPCR
2.4. Conditioned-Media Experiment
2.5. Reconstructed Three-Dimensional (3D) Skin Experiment
2.6. Human Clinical Trial
2.7. Statistical Analysis
3. Results and Discussion
3.1. Functional Study of Wrinkle-Related Gene SGPP2 in HaCaT Cells
3.2. Inflammation Exacerbates Skin Fibroblast-Mediated Wrinkle Formation
3.3. Screening Active Materials That Regulate SGPP2 Expression
3.4. Antiaging Effect of SGPP2 Expression-Regulating Materials In Vitro
3.4.1. Recovery of Inflammation-Induced Decrease in ECM-Related Gene in NHDFs
3.4.2. Effect of Enhanced Type I Procollagen Synthesis
3.5. Dermal Collagen Enhances the Efficacy of the Selected Materials within 3D Skin Model
3.6. Improvement in Skin Wrinkles and Pores Using LG Formula-Containing Materials That Modulated SGPP2 Expression
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, J.; Ye, S.; Jun, S.-H.; Kang, N.-G. Efficacy of SGPP2 Modulation-Mediated Materials in Ameliorating Facial Wrinkles and Pore Sagging. Curr. Issues Mol. Biol. 2024, 46, 9122-9135. https://doi.org/10.3390/cimb46080539
Kim J, Ye S, Jun S-H, Kang N-G. Efficacy of SGPP2 Modulation-Mediated Materials in Ameliorating Facial Wrinkles and Pore Sagging. Current Issues in Molecular Biology. 2024; 46(8):9122-9135. https://doi.org/10.3390/cimb46080539
Chicago/Turabian StyleKim, Juhyun, Sanghyun Ye, Seung-Hyun Jun, and Nae-Gyu Kang. 2024. "Efficacy of SGPP2 Modulation-Mediated Materials in Ameliorating Facial Wrinkles and Pore Sagging" Current Issues in Molecular Biology 46, no. 8: 9122-9135. https://doi.org/10.3390/cimb46080539