Gibberellins Play an Essential Role in the Bud Growth of Petunia hybrida
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Growth Conditions
2.2. Hormonal Application
2.3. Isolation of Genes
2.4. Analysis of Gene Expression
2.5. Arabidopsis Transformation and Phenotype Analysis
2.6. Data Analysis
3. Results
3.1. Exogenous GA3 Could Not Promote Axillary Bud Germination in Petunia
3.2. GA Promotes Bud Elongation
3.3. GA and CK Synergistically Promote Bud Elongation
3.4. GA Pathway Genes Respond to Decapitation
3.5. Cloning of PhGID1 and Sequence Analysis
3.6. Analysis of Tissue-Specific Expression of PhGID1
3.7. Overexpression of PhGID1 Increases Branch Number
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer Name | Primer Sequence (5′ to 3′) |
---|---|
PhGID1-F | ATGATCAATTTGCTTTTAGTACC |
PhGID1-R | CTATGAATGGTTAGGATGGATGA |
qRT-PhGID1-F | CGACGGTCACCTGAACATAG |
qRT-PhGID1-R | TTCTCGGATTCGGTCCTTTT |
PhGID1-1300-F | CCAAATCGACTCTAGAATGATCAATTTGCTTTTAGTACC |
PhGID1-1300-R | CCACTAGTATTTAAATGCTATGAATGGTTAGGATGGATGA |
PhBRC1-F | CCCATTTGCTCATCTTTATTC |
PhBRC1-R | CTGCCACTTTGCTTACTCATA |
PhDRM1-F | GAATGTGTGGAGGAGTGTTTTT |
PhDRM1-R | CCATATTGCACCCCCTTTTGTT |
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Deng, J.; Deng, X.; Yao, H.; Ji, S.; Dong, L. Gibberellins Play an Essential Role in the Bud Growth of Petunia hybrida. Curr. Issues Mol. Biol. 2024, 46, 9906-9915. https://doi.org/10.3390/cimb46090590
Deng J, Deng X, Yao H, Ji S, Dong L. Gibberellins Play an Essential Role in the Bud Growth of Petunia hybrida. Current Issues in Molecular Biology. 2024; 46(9):9906-9915. https://doi.org/10.3390/cimb46090590
Chicago/Turabian StyleDeng, Jichu, Xinyi Deng, Huanyu Yao, Shunhua Ji, and Lili Dong. 2024. "Gibberellins Play an Essential Role in the Bud Growth of Petunia hybrida" Current Issues in Molecular Biology 46, no. 9: 9906-9915. https://doi.org/10.3390/cimb46090590