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Article

Bioconversion of L-Tyrosine into p-Coumaric Acid by Tyrosine Ammonia-Lyase Heterologue of Rhodobacter sphaeroides Produced in Pseudomonas putida KT2440

1
Molecular Biotechnology Laboratory, Biotecnos S.A., Viña del Mar 2520000, Chile
2
Fermentations Laboratory, Biochemical Engineering School, Pontificia Universidad Católica de Valparaíso, Valparaíso 2340025, Chile
3
Biological Chemistry Laboratory, Chemistry Institute, Pontificia Universidad Católica de Valparaíso, Valparaíso 2340025, Chile
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2024, 46(9), 10112-10129; https://doi.org/10.3390/cimb46090603
Submission received: 19 July 2024 / Revised: 4 September 2024 / Accepted: 6 September 2024 / Published: 12 September 2024
(This article belongs to the Collection Feature Papers in Current Issues in Molecular Biology)

Abstract

p-Coumaric acid (p-CA) is a valuable compound with applications in food additives, cosmetics, and pharmaceuticals. However, traditional production methods are often inefficient and unsustainable. This study focuses on enhancing p-CA production efficiency through the heterologous expression of tyrosine ammonia-lyase (TAL) from Rhodobacter sphaeroides in Pseudomonas putida KT2440. TAL catalyzes the conversion of L-tyrosine into p-CA and ammonia. We engineered P. putida KT2440 to express TAL in a fed-batch fermentation system. Our results demonstrate the following: (i) successful integration of the TAL gene into P. putida KT2440 and (ii) efficient bioconversion of L-tyrosine into p-CA (1381 mg/L) by implementing a pH shift from 7.0 to 8.5 during fed-batch fermentation. This approach highlights the viability of P. putida KT2440 as a host for TAL expression and the successful coupling of fermentation with the pH-shift-mediated bioconversion of L-tyrosine. Our findings underscore the potential of genetically modified P. putida for sustainable p-CA production and encourage further research to optimize bioconversion steps and fermentation conditions.
Keywords: green chemistry; bioreactor design; fermentation optimization; bioprocess development; tyrosine ammonia-lyase; pseudomonas; p-hydroxycinnamic acid green chemistry; bioreactor design; fermentation optimization; bioprocess development; tyrosine ammonia-lyase; pseudomonas; p-hydroxycinnamic acid

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MDPI and ACS Style

Calderón, C.G.; Gentina, J.C.; Evrard, O.; Guzmán, L. Bioconversion of L-Tyrosine into p-Coumaric Acid by Tyrosine Ammonia-Lyase Heterologue of Rhodobacter sphaeroides Produced in Pseudomonas putida KT2440. Curr. Issues Mol. Biol. 2024, 46, 10112-10129. https://doi.org/10.3390/cimb46090603

AMA Style

Calderón CG, Gentina JC, Evrard O, Guzmán L. Bioconversion of L-Tyrosine into p-Coumaric Acid by Tyrosine Ammonia-Lyase Heterologue of Rhodobacter sphaeroides Produced in Pseudomonas putida KT2440. Current Issues in Molecular Biology. 2024; 46(9):10112-10129. https://doi.org/10.3390/cimb46090603

Chicago/Turabian Style

Calderón, Carlos G., Juan C. Gentina, Oscar Evrard, and Leda Guzmán. 2024. "Bioconversion of L-Tyrosine into p-Coumaric Acid by Tyrosine Ammonia-Lyase Heterologue of Rhodobacter sphaeroides Produced in Pseudomonas putida KT2440" Current Issues in Molecular Biology 46, no. 9: 10112-10129. https://doi.org/10.3390/cimb46090603

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