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Article

An In Vitro HL-1 Cardiomyocyte-Based Olfactory Biosensor for Olfr558-Inhibited Efficiency Detection

1
Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China
2
The MOE Frontier Science Center for Brain Science & Brain-Machine Integration, Zhejiang University, Hangzhou 310027, China
3
College of Life Sciences, Zhejiang University, Hangzhou 310058, China
4
Cancer Center, Binjiang Institute of Zhejiang University, Hangzhou 310027, China
5
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Chemosensors 2022, 10(6), 200; https://doi.org/10.3390/chemosensors10060200
Submission received: 22 April 2022 / Revised: 12 May 2022 / Accepted: 20 May 2022 / Published: 24 May 2022
(This article belongs to the Special Issue Advances in Analytical Systems for Gaseous Mixture)

Abstract

Some short-chain fatty acids with a pungent or unpleasant odor are important components of human body odor. These malodors severely threaten human health. The antagonists of malodors would help to improve odor perception by affecting the interaction between odors and their receptors. However, the traditional odor detection and analysis methods, such as MOS, electrochemical, conductive polymer gas sensors, or chromatography-mass spectrometry are not suitable for screening the antagonists since they are unable to detect the ligand efficacy after odor-receptor binding. In this study, RT-PCR results showed that HL-1 cardiomyocytes endogenously express the olfactory receptor 558 (Olfr558) which can be activated by several malodorous short-chain fatty acids. Therefore, an in vitro HL-1 cardiomyocyte-based olfactory biosensor (HCBO-biosensor) was developed by combining cardiomyocytes and microelectrode array (MEA) chips for screening the potential antagonists of the Olfr558. Firstly, it showed that the biosensor specifically responded to ligands of Olfr558 through odor stimulation experiments. Then, an odor response model of HL-1 cardiomyocytes was constructed by a ligand of Olfr558 (isovaleric acid). The response feature of the in vitro HCBO-biosensor to individual odors and mixtures with a potential antagonist (citral or β-damascenone) were extracted and compared. Finally, the Olfr558-inhibited efficiency was indirectly detected by comparing the half-maximal inhibitory concentration of isovaleric acid. The results showed that β-damascenone greatly inhibited Olfr558 while citral showed no significant inhibitory effect. In conclusion, we built a novel screening method for the antagonists of Olfr558 based on HL-1 cardiomyocytes and the MEA chip which will assist odor-related companies to develop novel antagonists of Olfr558.
Keywords: in vitro cell-based biosensor; olfactory receptor sensor; odor receptor-inhibited efficiency; cardiomyocytes; microelectrode array in vitro cell-based biosensor; olfactory receptor sensor; odor receptor-inhibited efficiency; cardiomyocytes; microelectrode array

Share and Cite

MDPI and ACS Style

Yuan, Q.; Qin, C.; Zhang, S.; Wu, J.; Qiu, Y.; Chen, C.; Huang, L.; Wang, P.; Jiang, D.; Zhuang, L. An In Vitro HL-1 Cardiomyocyte-Based Olfactory Biosensor for Olfr558-Inhibited Efficiency Detection. Chemosensors 2022, 10, 200. https://doi.org/10.3390/chemosensors10060200

AMA Style

Yuan Q, Qin C, Zhang S, Wu J, Qiu Y, Chen C, Huang L, Wang P, Jiang D, Zhuang L. An In Vitro HL-1 Cardiomyocyte-Based Olfactory Biosensor for Olfr558-Inhibited Efficiency Detection. Chemosensors. 2022; 10(6):200. https://doi.org/10.3390/chemosensors10060200

Chicago/Turabian Style

Yuan, Qunchen, Chunlian Qin, Saisai Zhang, Jianguo Wu, Yong Qiu, Changming Chen, Liquan Huang, Ping Wang, Deming Jiang, and Liujing Zhuang. 2022. "An In Vitro HL-1 Cardiomyocyte-Based Olfactory Biosensor for Olfr558-Inhibited Efficiency Detection" Chemosensors 10, no. 6: 200. https://doi.org/10.3390/chemosensors10060200

APA Style

Yuan, Q., Qin, C., Zhang, S., Wu, J., Qiu, Y., Chen, C., Huang, L., Wang, P., Jiang, D., & Zhuang, L. (2022). An In Vitro HL-1 Cardiomyocyte-Based Olfactory Biosensor for Olfr558-Inhibited Efficiency Detection. Chemosensors, 10(6), 200. https://doi.org/10.3390/chemosensors10060200

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