Nasopharyngeal and Peripheral Blood Type II Interferon Signature Evaluation in Infants during Respiratory Syncytial Virus Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients
2.2. mRNA Isolation and Real-Time PCR
2.3. IFN Signature Analysis
2.4. Statistical Analysis
3. Results
3.1. Study Populations
3.2. IFN-Stimulated Genes Expression Evaluated by qPCR
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variable | Bronchiolitis (30) * | Healthy Controls (36) * | p Value § |
---|---|---|---|
Age (days) | 86 ± 84 | 92 ± 101 | 0.585 |
White blood cells count (cells × 109/L) | 9670 ± 650 | 8830 ± 992 | 0.386 |
Neutrophils (cells × 109/L) | 2834 ± 1129 | 3228 ± 1010 | 0.545 |
Lymphocytes (cells × 109/L) | 5400 ± 1998 | 4180 ± 1883 | 0.032 ° |
Eosinophils (cells × 109/L) | 302 ± 100 | 443 ± 80 | 0.310 |
Monocytes (cells × 109/L) | 1503 ± 1030 | 632 ± 301 | <0.0001 ° |
CXCL9 | CXCL10 | Ido1 | IFN | IFN Score |
---|---|---|---|---|
0.157065 | 8.18543 | 0.286978 | 0.164163 | 0.22557 |
0.624713 | 0.679134 | 0.553085 | 0.197167 | 0.588899 |
0.061826 | 0.16016 | 0.069976 | 0.311058 | 0.115068 |
0.931124 | 2.576414 | 1.962246 | 0.549406 | 1.446685 |
0.587774 | 2.936548 | 4.420878 | 0.804047 | 1.870297 |
1.766501 | 3.628207 | 1.800975 | 0.693805 | 1.783738 |
0.302649 | 1.540725 | 1.108899 | 2.195808 | 1.324812 |
0.58293 | 1.376756 | 1.961242 | 0.326026 | 0.979843 |
0.937127 | 35.02627 | 1.534166 | 0.776836 | 1.235647 |
0.728682 | 143.6117 | 12.19011 | 1.500456 | 6.845283 |
0.09159 | 25.39361 | 2.204072 | 0.203047 | 1.203559 |
0.660717 | 51.14568 | 3.818783 | 0.229422 | 2.23975 |
0.068105 | 0.242614 | 0.109202 | 0.234683 | 0.171942 |
0.127123 | 0.490703 | 0.448894 | 0.170006 | 0.30945 |
0.457043 | 9.124063 | 0.856635 | 0.262696 | 0.656839 |
1.638153 | 6.903458 | 0.736292 | 0.049596 | 1.187223 |
2.700755 | 6.703653 | 0.173626 | 1.332774 | 2.016765 |
0.282434 | 0.369647 | 0.176321 | 0.295971 | 0.289203 |
0.470063 | 14.85163 | 1.255824 | 1.693257 | 1.474541 |
0.802751 | 270.6202 | 3.105014 | 0.606671 | 1.953883 |
0.229872 | 3.890983 | 0.424244 | 0.382459 | 0.403351 |
0.693212 | 4.8035 | 0.830444 | 0.321862 | 0.761828 |
0.159718 | 0.358076 | 0.063926 | 0.44009 | 0.258897 |
0.372456 | 1.831377 | 0.710723 | 0.885488 | 0.798105 |
1.423442 | 1.934601 | 0.320828 | 1.820754 | 1.622098 |
0.276544 | 1.094884 | 0.69411 | 0.886863 | 0.790487 |
0.189038 | 0.331033 | 0.059418 | 0.266275 | 0.227656 |
0.060295 | 0.15324 | 0.119145 | 0.968841 | 0.136193 |
0.174432 | 0.771439 | 0.319235 | 0.974291 | 0.545337 |
0.45667 | 13.39745 | 0.651593 | 0.98962 | 0.820607 |
CXCL9 | CXCL10 | Ido1 | IFN | IFN Score |
---|---|---|---|---|
0.927221 | 5.927591 | 2.814915 | 1.059956 | 1.937436 |
0.807148 | 0.578726 | 1.007283 | 0.800781 | 0.803965 |
0.961704 | 2.670438 | 0.346059 | 0.938156 | 0.94993 |
0.553646 | 3.473331 | 2.624912 | 1.69073 | 2.157821 |
0.558547 | 3.529864 | 1.485558 | 0.880558 | 1.183058 |
1.166531 | 6.642831 | 3.234286 | 1.094232 | 2.200409 |
0.574403 | 0.930456 | 0.491075 | 0.977094 | 0.752429 |
0.703173 | 3.167205 | 2.206281 | 1.306828 | 1.756554 |
0.593752 | 2.681653 | 2.472985 | 1.178443 | 1.825714 |
0.526364 | 2.241326 | 2.699156 | 0.94144 | 1.591383 |
0.789334 | 16.15611 | 1.675641 | 0.349211 | 1.232488 |
0.449238 | 1.028112 | 1.120321 | 2.891309 | 1.074216 |
3.026584 | 33.28678 | 3.15599 | 4.782118 | 3.969054 |
1.415051 | 6.074084 | 2.684512 | 1.954509 | 2.31951 |
1.233968 | 5.30949 | 3.012727 | 2.855546 | 2.934137 |
1.818892 | 54.26082 | 2.671744 | 1.829073 | 2.250408 |
1.482601 | 7.572696 | 3.070401 | 5.337788 | 4.204095 |
0.198741 | 1.123516 | 7.019939 | 1.755256 | 1.439386 |
0.246297 | 2.135114 | 2.707466 | 1.617702 | 1.876408 |
0.315997 | 3.798261 | 1.361492 | 0.123542 | 0.838745 |
0.547174 | 2.802106 | 3.099739 | 2.73358 | 2.767843 |
0.299992 | 31.17147 | 14.73604 | 5.374277 | 10.05516 |
0.62112 | 1.344691 | 3.800596 | 1.08666 | 1.215676 |
0.927419 | 12.62865 | 9.230163 | 1.47954 | 5.354851 |
0.525293 | 3.374039 | 2.958201 | 2.699701 | 2.828951 |
0.473346 | 7.375175 | 1.825008 | 1.997246 | 1.911127 |
0.831074 | 5.225671 | 0.523769 | 1.607206 | 1.21914 |
0.667976 | 1.468509 | 0.821468 | 0.810344 | 0.815906 |
1.640676 | 11.53084 | 1.537516 | 2.962262 | 2.301469 |
4.472612 | 44.21708 | 10.61164 | 8.757177 | 9.684408 |
0.422769 | 1.28918 | 1.027396 | 2.246802 | 1.158288 |
0.554591 | 2.148308 | 2.335841 | 0.387631 | 1.35145 |
4.242832 | 20.40956 | 2.606556 | 4.880706 | 4.561769 |
0.927105 | 3.324605 | 0.747185 | 3.002083 | 1.964594 |
1.444508 | 2.796676 | 1.462326 | 3.054695 | 2.129501 |
0.192616 | 0.748958 | 0.230515 | 1.446799 | 0.489737 |
IFNg | CXCL9 | CXCL10 | IDO1 | IFN Score |
---|---|---|---|---|
1.685659 | 0.106575 | 0.060205 | 0.190854 | 0.177204 |
0.199817 | 0.450834 | 0.357298 | 0.520083 | 0.450208 |
0.6063 | 0.114727 | 0.115976 | 2.218294 | 0.847529 |
2.364469 | 1.466056 | 1.387023 | 3.230586 | 1.915262 |
1.629892 | 0.515913 | 0.334127 | 1.168483 | 0.884869 |
2.218103 | 0.494326 | 0.552003 | 2.591719 | 2.211611 |
0.742426 | 3.30203 | 7.521585 | 61.60443 | 5.878039 |
0.288555 | 0.349889 | 0.12762 | 0.980016 | 0.319222 |
0.937276 | 0.708254 | 0.318651 | 3.882854 | 0.822765 |
2.629424 | 3.740852 | 2.294862 | 25.64459 | 4.465884 |
8.459833 | 0.68337 | 2.678824 | 24.66566 | 3.105226 |
0.116979 | 0.333878 | 0.191974 | 0.371062 | 0.262926 |
1.939566 | 1.338197 | 0.955549 | 12.77733 | 1.638882 |
4.688201 | 0.820236 | 1.056642 | 30.713 | 3.659446 |
// | 0.711009 | 0.875694 | 7.346527 | 2.825088 |
0.042141 | 0.051571 | 0.025111 | 0.01295 | 0.046856 |
0.357725 | 0.264255 | 0.155025 | 3.177838 | 0.36583 |
2.861291 | 1.714959 | 2.135358 | 14.25268 | 2.485898 |
2.379219 | 0.3004 | 0.203204 | 2.950032 | 0.754847 |
0.989339 | 2.618225 | 0.873043 | 3.202769 | 2.563137 |
0.05656 | 0.025731 | 0.016338 | 0.130882 | 0.062945 |
0.590727 | 1.039541 | 0.494817 | 1.226243 | 0.764687 |
// | 1.674171 | 0.778514 | 2.732303 | 1.142535 |
1.114745 | 0.356293 | 1.091995 | 18.2477 | 1.293056 |
3.358902 | 1.725384 | 1.169357 | 2.415619 | 2.123589 |
// | // | // | 7.065799 | 3.783915 |
0.033014 | 0.017777 | 0.028004 | 2.806203 | 0.071856 |
3.286032 | 1.858926 | 9.757487 | 45.052 | 4.096859 |
// | // | // | 2.414923 | 1.316748 |
// | 0.060658 | 0.041742 | // | 0.052629 |
// | 0.159294 | 0.03838 | 1.285106 | 0.159294 |
2.72743 | 1.535855 | 0.419061 | 1.133716 | 0.93273 |
1.111062 | 0.075519 | 0.066413 | // | 0.252402 |
0.228895 | 0.020628 | 0.01378 | 1.211771 | 0.148608 |
0.772693 | 0.185458 | // | 0.599208 | 0.599208 |
1.345381 | 0.09437 | 0.119168 | // | 0.125718 |
3.47402 | 0.311783 | 0.234453 | 2.285236 | 0.536745 |
// | 1.710044 | 0.532687 | 1.712725 | 1.711384 |
// | // | 0.124275 | 4.711086 | 0.617166 |
// | // | 0.164969 | 4.244882 | 0.364803 |
// | 0.272361 | 1.167929 | 6.361056 | 0.878366 |
// | 0.672684 | 0.285985 | 0.403993 | 0.403993 |
// | // | 0.262111 | // | 0.229 |
// | // | 0.08179 | // | 0.134591 |
1.749853 | 0.583862 | 0.332689 | 0.660082 | 0.83433 |
IFNg | CXCL9 | CXCL10 | IDO1 | IFN Score |
---|---|---|---|---|
0.119396 | 0.365331 | 0.212938 | 0.454007 | 0.351945 |
// | // | // | 4.354237 | 3.21977 |
2.396478 | 3.402945 | 11.74381 | 21.757 | 4.622528 |
1.084336 | 0.501719 | 0.324502 | 0.174045 | 0.396846 |
1.370674 | 1.186268 | 0.581905 | 0.50408 | 0.732686 |
1.941057 | 1.744747 | 0.761611 | 0.479918 | 0.777233 |
0.900624 | 1.081239 | 0.490691 | 0.45876 | 0.530976 |
// | 0.067341 | 0.494851 | // | 0.494851 |
0.570475 | 0.233015 | 0.373023 | 0.449391 | 0.449391 |
// | 6.473846 | 7.796893 | // | 4.625882 |
4.727643 | 4.158529 | 2.109328 | // | 3.227281 |
0.478495 | 0.471916 | 1.254819 | 3.87319 | 0.919472 |
0.12972 | 0.0641 | 0.110914 | 2.88078 | 0.199274 |
3.129895 | 1.368325 | 1.13171 | 1.313043 | 1.222377 |
0.485004 | 1.065 | 0.874331 | 1.552961 | 1.132574 |
0.78607 | 1.009494 | 0.807926 | 0.295926 | 0.7852 |
1.857269 | 1.117105 | 0.639395 | 0.215011 | 0.936784 |
0.320975 | 1.104835 | 3.47792 | 3.986919 | 1.145811 |
0.84222 | 1.283846 | 0.833621 | 1.326317 | 1.063033 |
1.786934 | 3.155041 | 2.117417 | 0.710685 | 1.805562 |
7.505436 | 7.622924 | 4.274014 | 0.492337 | 4.072474 |
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Savino, F.; Dini, M.; Clemente, A.; Calvi, C.; Pau, A.; Galliano, I.; Gambarino, S.; Bergallo, M. Nasopharyngeal and Peripheral Blood Type II Interferon Signature Evaluation in Infants during Respiratory Syncytial Virus Infection. Medicina 2024, 60, 259. https://doi.org/10.3390/medicina60020259
Savino F, Dini M, Clemente A, Calvi C, Pau A, Galliano I, Gambarino S, Bergallo M. Nasopharyngeal and Peripheral Blood Type II Interferon Signature Evaluation in Infants during Respiratory Syncytial Virus Infection. Medicina. 2024; 60(2):259. https://doi.org/10.3390/medicina60020259
Chicago/Turabian StyleSavino, Francesco, Maddalena Dini, Anna Clemente, Cristina Calvi, Anna Pau, Ilaria Galliano, Stefano Gambarino, and Massimiliano Bergallo. 2024. "Nasopharyngeal and Peripheral Blood Type II Interferon Signature Evaluation in Infants during Respiratory Syncytial Virus Infection" Medicina 60, no. 2: 259. https://doi.org/10.3390/medicina60020259