Screening for Small Molecule Modulators of Trypanosoma brucei Hsp70 Chaperone Activity Based upon Alcyonarian Coral-Derived Natural Products

Abstract

The Trypanosoma brucei Hsp70/J-protein machinery plays an essential role in survival, differentiation, and pathogenesis of the protozoan parasite, and is an emerging target against African Trypanosomiasis. This study evaluated a set of small molecules, inspired by the malonganenones and nuttingins, as modulators of the chaperone activity of the cytosolic heat inducible T. brucei Hsp70 and constitutive TbHsp70.4 proteins. The compounds were assessed for cytotoxicity on both the bloodstream form of T. b. brucei parasites and a mammalian cell line. The compounds were then investigated for their modulatory effect on the aggregation suppression and ATPase activities of the TbHsp70 proteins. A structure–activity relationship for the malonganenone-class of alkaloids is proposed based upon these results.

1. Introduction

The etiological agent of African Trypanosomiasis, Trypanosoma brucei (T. brucei), is an extracellular blood- and tissue-borne unicellular parasitic protozoan. It gives rise to infection in both humans and animals, predominantly across sub-Saharan Africa, and is transmitted to its mammalian host during a blood meal of the infected tsetse fly vector (Glossina spp.), which ensures the cyclical transmission of the parasite between numerous hosts [1]. There is a dire need for the development of more effective and safer drugs to treat the disease, because of the toxicity and long duration for the current treatments, coupled to the increase of drug resistance in trypanosomes and the lack of a vaccine [2,3]. Molecular chaperones have been shown to play an essential role in stress-induced stage differentiation and are vital for disease progression and transmission [4,5], making this protein family an attractive anti-parasitic chemotherapeutic target.

The highly ubiquitous 70-kDa heat shock protein (Hsp70) family of molecular chaperones, known as HSPA in humans, is one of the most evolutionarily conserved protein families. It is involved in a plethora of essential cellular functions that include promoting the correct protein folding of newly synthesized polypeptides, mediating protein translocation, and facilitating proteolytic degradation of non-native and aggregated proteins [6,7]. The domain architecture of eukaryotic cytosolic Hsp70s is typically comprised of an N-terminal nucleotide binding domain connected via a linker region to a C-terminal domain with a substrate binding domain, and a 10-kDa α-helical domain with a conserved EEVD motif [8,9]. The function and specificity of Hsp70s are regulated by the 40-kDa heat shock protein (Hsp40) family, also referred to as J-proteins, due to the presence of their signature domain, the conserved ~70 amino acid region known as the J-domain [10], which interacts with the nucleotide binding domain of Hsp70. J-proteins function as a co-chaperone of Hsp70 by delivering specific substrates and stimulating the low intrinsic ATPase activity of Hsp70 [10]. J-proteins are classified into four types, with types I and II binding protein and preventing aggregation of unfolded proteins, thereby displaying a holding-function [11].

The Trypanosoma brucei Hsp70 (TbHsp70) and J-protein families have undergone greater evolutionary expansion relative to other eukaryotic systems, and contain diverse family members [12]. RNAi-mediated knockdown of T. brucei genes conducted by Alsford and colleagues [13] demonstrated that the Hsp70/J-protein machinery plays a prominent role in trypanosome biology, as the loss of certain members of these protein families was found to be lethal at one or more stages in its life cycle. It has been proposed that TbHsp70 plays an essential role in cytoprotection during cellular stress [12], and in vivo studies on the Type I cytosolic J-protein, Tbj2, have shown that it is stress inducible and essential [14]. Furthermore, evidence from in vitro assays [15] suggested that Tbj2 has chaperone (e.g., able to suppress protein aggregation of model substrates) and co-chaperone properties (e.g., able to stimulate the ATPase activity of a trypanosomal Hsp70).

Several promising studies have been conducted on assessing the potential of naturally occurring marine- or plant-based extractables as modulators of the Hsp70 chaperone system in Plasmodium falciparum (P. falciparum) [16,17,18,19,20]. Cockburn and colleagues [18] investigated a set of small molecules derived from two classes of compounds, 1,4-naphthoquinones and marine prenylated alkaloids, for modulation of the activity of two biologically important plasmodial Hsp70s. One of the compounds, malonganenone A, showed desirable properties as a plasmodial Hsp70 modulator, as the compound inhibited the steady-state and J-protein stimulated ATPase activity of plasmodial Hsp70s, and not that of human Hsp70 [18]. It was also shown to disrupt the interaction between the exported PfHsp70-x and J-protein, marking malonganenone A for further study particularly with the synthesis of analogues that have more potent antimalarial activities and higher selectivity as PfHsp70 inhibitors [18].

The malonganenones are a family of tetraprenylated alkaloid marine natural products isolated from gorgonian sea fans, collected in Africa and China [21,22,23,24]. To date, a total of 17 malonganenones (A–Q) have been isolated, as well as six closely related nuttingins (A–F) (Figure 1). The malonganenones and nuttingins are cytotoxic against several cancer cell lines (IC₅₀ 0.35–84.9 μM) [21,23] while malonganenones D–H and nuttingins A–F cause apoptosis of transformed mammalian cells (1.25 μg/mL) [22]. Additionally, malonganenones L and Q are inhibitory against phosphodiesterase-4D (IC₅₀ 8.5 and 20.3 μM) [24] and malonganenone D reduces c-Met kinase activity 2-fold (10 μM) [23]. Importantly, malonganenones A and C are anti-plasmodial against P. falciparum (IC₅₀ 0.81 and 5.20 μM) [17].

The malonganenones vary mainly in the composition of the nitrogenous head group, with small changes at the end of the prenyl side chain. Bioactivity mainly varies relative to the identity of the head group, suggesting that they play the primary role of pharmacophore. Therefore, a simpler prenyl chain, as in malonganenone J, could be substituted for the other natural product side chains, and still be expected to present useful bioactivity. Although the side chain may be less important for bioactivity, it still plays a significant role. A simplified analogue of malonganenone B, which substituted the side chain with a methyl group, was completely inactive in the same assay against PfHsp70-1, whereas the original compound’s activity was dose-dependent [17]. This result suggests that an extended side chain could be important for adding lipophilicity. Therefore, in this study, a structure–activity relationship (SAR) of the side chain length was probed by varying the length from one to three prenyl units. Analogues of the malonganenone and nuttingins were synthesized by alkylation of N-heterocyclic compounds, while analogues of malonganenone C were synthesized by simple derivatization of terpenoid amines. It should be noted that malonganenone J is the only member of the family to have succumbed to total synthesis to date [25].

Overall, this study aimed to evaluate potential inhibitors, inspired by the malonganenones and nuttingins, of the chaperone activity of T. brucei Hsp70 proteins. The compounds were assessed for cytotoxicity on both the bloodstream form of T. b. brucei parasites and a mammalian cell line. The compounds were then investigated for their modulatory effect on the aggregation suppression and ATPase activities of cytosolic TbHsp70 (homologue of the cytosolic inducible human Hsp70, HSPA1A) and TbHsp70.4 (homologue of the constitutive human Hsp70, HSPA8). The human chaperone HSPA8 and its co-chaperone DNAJB2 (Hsj1a), a Type II J-protein, were chosen as model representatives for investigation of the modulatory effect of the small molecules on a human Hsp70/J-protein partnership. HSPA8 has been shown to be involved in essential housekeeping functions [26,27], as knockout of the gene in mice was shown to be lethal [28], and RNAi-mediated knockdown resulted in massive cell death in various cell types [29]. DNAJB2 has been shown to be preferentially expressed in neuronal cells [30], where it plays a prominent role in protein degradation [31], and it has been shown to stimulate the in vitro basal ATPase activity of HSPA8 [32], HSPA1A [16,18,33], PfHsp70-1 [16,18], and PfHsp70-x [18]. The intention was to identify inhibitors that were specific to modulating the activities of the trypanosomal Hsp70s, TbHsp70 and/or TbHsp70.4. Overall, this study aimed to identify a potential new avenue to African trypanosomiasis chemotherapy.

2. Results and Discussion

2.1. Synthesis of Malonganenone and Nuttingin Analogues

Coverage of chemical space was maximized for the first generation of malonganenone and nuttingin analogues by using non-selective alkylation conditions and terpenoid bromides, with geometric mixtures at the C-2 alkene, to give maximal compound diversity. These conditions tended to efficiently yield multiple regio- and geometric isomers per reaction. The regioisomers were easily separated by chromatography, but the geometric isomers proved to be more difficult. The (2E)- and (2Z)-isomers of the farnesyl derivatives were often isolated as mixtures and were tested as such. The geometric isomers of the geranylgeranyl derivatives were markedly easier to separate by chromatography.

The purines, adenine (1), 6-(dimethylamino) purine (2), and 6-chloropurine (3) were alkylated with prenyl bromides (Scheme 1). Alkylation of 1 yielded N-3- (4, 6) and N-9 (7, 8) monoalkylated products. Alkylation with geranyl bromide produced a minor amount of a dialkylated species (5), which was not isolated from the other reactions. Alkylation of 2 also yielded N-3- (10, 12) and N-9 (9, 11, 13, 14) monoalkylated regioisomers. The ¹H NMR spectra of 9–14 had broad N-methyl resonances, which were only equivalent for the N-9 isomers. This was rationalized by the formation of a stable imine resonance form for the N-3 regioisomer, which has also been observed previously for a similar N-3 alkylated 6-(dimethylamino)purine [34]. Alkylation of 3 yielded N-9- (15, 17, 19), and N-7 (16, 18, 20, 21) monoalkylated regioisomers.

Head groups based on purinones were also alkylated since the head group of nine malonganenones comprise a hypoxanthine core, with a carbonyl at C-6. Xanthine (22), 3-methylxanthine (23), and theophylline (24) are related by increasing methylation; theophylline is the head group of nuttingin A and B. The other two purinones selected were 2-mercapto-3-methylhypoxanthine (25) and 1,3-dimethyluric acid (26). Alkylation of the methyl series of purinones, and 25, all yielded N-7 monoalkylated regioisomers (29–41), except for 22, which only yielded N-3,N-7 dialkylated species (27, 28) (Scheme 2).

Alkylation of 26 yielded C-5 monoalkylated regioisomers (42, 43) and only a minor amount of N-9 monoalkylated regioisomer (44) from the farnesyl bromide reaction (Scheme 3). We suggest that these species result from enolate reactivity, providing a simple method for forming quaternary carbon-carbon bonds (Scheme 4).

Alkylation of 45 yielded N-5 monoalkylated analogues (46, 49), as well as N-1, N-5- (47, 50) and N-2, N-7 (48) dialkylated analogues, presumably from enhanced nucleophilicity of the pyrimidine ring (Scheme 5).

Other readily available N-heterocyclic compounds were also alkylated, including the pyrimidines, uracil (51), thymine (52), and cytosine (53). Alkylation of 51, and 52 yielded only N-1 monoalkylated derivatives (54–59) whereas 53 only produced N-1,N-3 dialkylated products (61, 63) (Scheme 6). In addition to the dialkylated cytosines, formylated imine derivatives (60, 62, 64) were also isolated, likely formed via the reaction solvent, DMF. Although formylation with DMF usually requires pre-activation, such as in the Vilsmeier–Haack reaction [35], milder conditions have also been successful at yielding formylated derivatives. For example, moderate heating of DMF and imidazole formylated a variety of amino acids and primary amines [36]. The proposed mechanism suggests that imidazole acts as an intermediate acyl transfer reagent (Scheme 7), therefore it is feasible that cytosine could also fulfil this role. The formyl imidazole produced is itself further reactive when other amine nucleophiles are present. The absence of stronger nucleophiles in these alkylation reactions resulted in the isolation of formyl cytosine.

Alkylation of potassium phthalimide (65) (Scheme 8) and imidazole (66) (Scheme 9) furnished the last of the N-heterocyclic analogues. Both reactions proceeded straightforwardly to yield N-1 monoalkylated analogues (67–72).

Malonganenones C, H, and K, have a simple formamide head group and analogues were synthesized by formylation [37,38] or acetylation [39,40] of prenyl amines [41], followed by methylation [42] (Scheme 10). The ¹H NMR spectra of the formamide- (73–77) and N-methyl formamide (78–80) series suggested a mixture of two rotamers, with doubling of most resonances near the head group. These observations are consistent with previous data reported for geranyl formamide [43] and of more relevance, malonganenone B [37]. Conversely, the acetamide analogues (81–85) were not rotameric but became so upon N-methylation (86–88), analogous to DMF.

2.2. Evaluation of the Potential Small Molecule Inhibitors for Cytotoxicity on Mammalian Cells and Parasites

A resazurin-based cytotoxicity assay was conducted to assess the potential anti-trypanosomal activity of the 74 compounds (Figure S1). Seven were identified to be non-toxic as the parasite survival was ≥100% (Figure S1).

The compounds were further assessed for toxicity using HeLa (human cervix adenocarcinoma) cells. Emetine is a natural alkaloid that has been shown to induce apoptosis in mammalian cell lines [44] and was incorporated into the study as a positive control, displaying high toxicity at 10 μM towards HeLa cells (6.03% cell viability). Compounds 5, 61, and 62 were toxic to HeLa cells at 20 μM (Figure S2). Comparison of the toxicity of the compounds at 20 μM toward both trypanosomes and HeLa cells indicates that the toxicity of most compounds is selective towards the parasite (Figure S2).

Most of the synthetic compounds were shown to display high anti-trypanosomal activity; 48 compounds were selected for further assessment as they reduced parasite growth ≥80% at 20 µM (Figure S1). Dose–response curves of the 48 selected compounds were generated, and the IC₅₀ (50% inhibitory concentration) values for each compound was determined (

Table 1. IC₅₀ values of selected compounds toward T. b. brucei parasites cultured in vitro. Drug standard pentamidine IC₅₀ 5.3 nM.

Compound	IC50/μM	Compound	IC50/μM	Compound	IC50/μM
6	12.7	28	1.2	59	15.8
7	47.7	29	18.9	60	4.9
8	13.4	30	4.6	63	2.1
9	28.4	31	1.9	68	32.2
11	9.5	32	5.9	69	9.2
12	14.0	33	10.2	71	26.8
13	8.7	36	5.2	72	3.4
14	9.2	37	21.6	74	27.3
15	12.2	42	10.9	76	11.0
16	11.0	44	16.6	77	41.8
17	12.9	47	1.2	80	41.2
18	9.3	48	5.5	82	4.5
19	6.8	49	16.7	83	21.5
20	11.0	50	2.1	84	2.2
21	19.1	55	16.5	85	3.1
27	2.0	56	6.2	88	38.4

, Table S1). For comparative purposes, pentamidine, an existing drug used to treat the early stages of Human African trypanosomiasis caused by T. b. gambiense [45,46] was used as the positive control.

Although none of the tested compounds are comparable to the drug standard pentamidine in potency, tentative SAR can be proposed from the results to aid in designing a second generation of analogues. SAR analysis of the side chain suggests that length correlates positively with activity as most of the shortest side chain derivatives are inactive and activity increases upon lengthening the side chain from farnesyl to geranylgeranyl (i.e., 7 vs. 8, 55 vs. 56, 68 vs. 69, 71 vs. 72). The activity is further influenced by the head group. Activating head groups have shorter optimal chain lengths and further lengthening has minimal effect (i.e., 11 vs. 14, 18 vs. 20, 21) whereas the deactivating head group series are completely inactive (i.e., 38–41), or activity is only rescued at the longest side chain length (i.e., 59, 37). No general trends can be derived from the identity of the head group, but small changes such as methylation seem to have a large effect on activity. For example, 3-methylxanthine (23) and theophylline (24) differ by the presence of an N-1 methyl and while all side chain lengths of the N-7 alkylated 3-methylxanthines (29–33) are active, only the N-7 geranylgeranyl theophyllines (36, 37) display activity. Converse to N-methyl deactivation, the acetamides are all more active than the formamides by 5- to 13-fold (74 vs. 82, 76 vs. 84, 77 vs. 85).

The SAR pertinent to alkene geometry generally suggests that the (2Z)-isomers or (2Z/E)-mixtures are more active than the (2E)-isomers, where the latter is inactive (i.e., 74 vs. 75) or activity is reduced by 1.5- to 5-fold (i.e., 36 vs. 37, 82 vs. 83, 84 vs. 85).

Of the 48 compounds screened, 22 compounds were shown to display high toxicity towards parasites with IC₅₀ values all below 10 µM (Table 1, Table S1). However, the positive control pentamidine was shown to display the highest toxicity towards the parasite (IC₅₀ 5.3 nM). Despite this, pentamidine is limited to treating the haemolymphatic stage of T. b. gambiense infections [47], and pentamidine resistance has been reported [48]. From these 22 compounds, the top five active compounds (28, 47, 31, 27, and 63), and two further compounds (60 and 48) with abundantly available mass, were selected for further assessment. Even though the mechanism of inhibition of the parasites by the selected compounds is currently unknown, their modulatory effect on the molecular chaperone activity of TbHsp70 proteins was evaluated.

2.3. Modulation of the Aggregation Suppression Activity of TbHsp70 and TbHsp70.4

The malate dehydrogenase (MDH) aggregation suppression assay was used to evaluate the modulatory effect of the seven selected compounds, that were highly toxic towards the parasites with IC₅₀ values all below 7 μM (27, 47, 48, 31, 28, 60, and 63), on the chaperone activities of TbHsp70 and TbHsp70.4. Human HSPA8 was not suitable for MDH aggregation suppression experiments, as the protein is prone to aggregation at 48 °C (Figure S3). The target compounds (in the absence of the Hsp70) were shown to have no effect on the aggregation of MDH (Figure S4), and thus were ruled out as chemical chaperones. DMSO (1% v/v) had no significant effect on the chaperone activity of both T. brucei Hsp70s (not shown). Increasing concentrations of the compounds resulted in a general increase in % MDH aggregation due to inhibition of the aggregation suppression activity of TbHsp70 and TbHsp70.4 (Figure 2A,B). Peptide substrate motifs recognized by Hsp70s have been described as having a central hydrophobic region of four to five residues, flanked by basic residues [49]. It is possible that the hydrophobic hydrocarbon side chains of the compounds are binding to the hydrophobic pocket of the substrate binding domains of the T. brucei Hsp70s, preventing MDH from binding.

2.4. The Basal ATPase Activities of TbHsp70, TbHsp70.4 and HsHSPA8 Can Be Differentially Modulated

An initial screen of the modulatory effects of the selected compounds (27, 47, 48, 31, 28, 60, and 63) on the basal ATPase activity of the TbHsp70s was conducted at single concentrations (300 μM) (Figure S6). DMSO (1% v/v) was also shown to have no significant effect on the ATPase activity of both T. brucei Hsp70s and HsHSPA8 (Figure S6). The compounds inhibited the basal ATPase activities of TbHsp70 and TbHsp70.4 to different extents. In comparison to TbHsp70.4, 31, 28, and 60 inhibited the basal ATPase activity of TbHsp70 to a lesser extent, with 60 being the least effective with 39% inhibition (Figure S6). These molecules may have a selective mode of inhibition through interaction with specific residues of the targeted domain in TbHsp70.4. Compounds which reduced the basal ATPase activities of the TbHsp70s by ≥70% at 300 μM (Figure S6) were further tested over a range of concentrations (Figure 3). The selected compounds inhibited the basal ATPase activities of TbHsp70.4 and TbHsp70 in dose-dependent manners (Figure 3). Compound 47 inhibited the ATPase activities to the greatest extent for both TbHsp70s, with the lowest concentration of 1 μM inhibiting the basal ATPase activity of TbHsp70.4 by 66% and TbHsp70 by 44%, respectively. Due to limited availability, only compounds 27, 28, 31, 47, and 63 were further assessed for inhibition of the basal ATPase activity of HsHSPA8 over a range of concentrations. The degree of inhibition of the basal HsHSPA8 ATPase activity by the same compounds was reduced in comparison to the TbHsp70s (Figure 3). The contrast in inhibition between the trypanosomal and human Hsp70 proteins may be due to a greater binding affinity of the compounds to the T. brucei Hsp70s than to human HSPA8.

2.5. Modulation of the J-Protein-Stimulated ATPase Activities of TbHsp70, TbHsp70.4 and HsHSPA8

Due to the promising results obtained, the remaining available compounds (27, 28, 31, 47, and 63) were investigated for modulation of J-stimulated ATPase activity of the T. brucei Hsp70s, and possible disruption of the Hsp70/J-protein partnerships. A preliminary screen of the modulatory effects of the five compounds at 300 μM was performed on the Tbj2-stimulated ATPase activities of TbHsp70 and TbHsp704 and the HsDnaJB2-stimulated ATPase activity of HsHSPA8 (Figure S7). DMSO (1% v/v) was also shown to have no significant effect on the J-stimulated ATPase activity of both T. brucei Hsp70s and HsHSPA8 (Figure S7). Tbj2 stimulated the ATPase activities of TbHsp70 3.23-fold and TbHsp70.4 2.98-fold, whilst HsDNAJB2 stimulated the ATPase activity of HsHSPA8 ~5-fold (data not shown). All the tested compounds were shown to inhibit the J-stimulated ATPase activities of TbHsp70 and TbHsp70.4 by ~60% and inhibit the J-stimulated ATPase activity of HsHSPA8 by ~40% (Figure S7) The addition of increasing concentrations of the compounds (27, 28, 31, 47, and 63) reduced the J-stimulated ATPase activities of TbHsp70 and TbHsp70.4, with the greatest reduction observed for TbHsp70 (Figure 4). The compounds tested were also shown to suppress the DNAJB2-stimulated ATPase activity of HsHSPA8 (Figure 4). The small molecules were shown to have a decreased effect on the J-stimulated ATPase activity of HsHSPA8 in comparison to the TbHsp70s (Figure 4), though the results indicate that Tbj2 is stimulating the compound-induced inhibition of the basal ATPase activity.

An additional experiment was carried out to assess the effect of varying the order of addition of the compounds (27, 28, 31, 47, and 63) at single concentrations (150 μM) and Tbj2 in the assay. This was conducted to elucidate if the small molecules were disrupting the Hsp70/J-protein partnership. However, no significant difference was observed in the inhibition of the J-stimulated ATPase activity for both T. brucei Hsp70s by varying the order of addition of the reaction components (p < 0.05; Figure 5). This suggests that the small molecules are not disrupting the binding of Tbj2 to the TbHsp70s but are binding to a site, independent of the Tbj2 binding site.

3. Materials and Methods

3.1. General Synthetic Procedures

All reactions were carried out under an inert atmosphere (Ar or N₂), with oven or vacuum-dried glassware, using standard syringe techniques and dry solvents. Dry DCM, Et₂O, and THF were obtained from a Puresolv. system (Innovative Technology). Triethylamine was distilled from CaH₂. Methyl iodide was run through a plug of activated alumina prior to use. 2-Mercapto-3-methylhypoxanthine [50], the relevant terpenoid bromides [51,52,53], and terpenoid amines [41] were synthesized according to literature. The C-2 alkene E/Z ratio of the terpenoid bromides was determined from integrated peak areas detected by ¹H NMR spectroscopy. Geranyl bromide was entirely the (2E)-isomer as set by the (2E)-configuration of the geraniol starting material. Farnesyl bromide was synthesized from an isomeric mixture of farnesol, with an E/Z ratio of 3:2. Geranylgeranyl bromide was synthesized from racemic geranyllinalool, with the resulting E/Z ratio of 3:1. All other solvents and reagents were used as received from commercial suppliers. All compounds were purified by silica gel flash chromatography, using silica gel 60 (40–63 micron), unless otherwise stated. Thin-layer chromatography was performed on Macherey-Nagel, POLYGRAM^® Sil G/UV254 plates, and were visualized with a UV lamp, iodine-, ceric ammonium molybdate-, vanillin-, or sulfuric acid stain. 1D (¹H, ¹³C, NOESY) and 2D (COSY, HSQC, HMBC) NMR spectra were recorded using Varian Inova or DirectDrive instruments operating at 300 (Inova), 500 (Inova), or 600 (DirectDrive) MHz for proton and 125 or 150 MHz for carbon. IR spectra were obtained using an ALPHA FT-IR spectrometer (Bruker). MS data and tandem MS data were obtained using an Agilent 6530 Q-TOF LC/MS high-resolution mass spectrometer equipped with an Agilent 1260 HPLC system for sample introduction.

3.2. General Alkylation Procedure for Synthesis of 4–21, 27–50, 54–64, 67–72

3.2.1. (E)-3-(3,7-Dimethylocta-2,6-dien-1-yl)-3H-purin-6-amine (4) and 3,9-bis((E)-3,7-dimethylocta-2,6-dien-1-yl)-3,9-dihydro-6H-purin-6-imine (5)

Adenine (1.00 mmol, 134.7 mg) and K₂CO₃ (1.31 mmol, 181.3 mg) were stirred in DMF (2 mL) at RT for 10 min before dropwise addition of geranyl bromide (1.2 mmol, 250.6 mg). The reaction was stirred for 21 h, then poured onto H₂O (6 mL) and extracted with EA (3 × 2 mL). The combined extracts were washed with H₂O (3 × 2 mL), then brine (1 × 2 mL) and dried over anhydrous MgSO₄. The dried residue was purified by silica gel flash chromatography (5% MeOH/EA) to yield 4 and 5.

Compound 4: 51.9 mg (19%), pale-yellow crystals; R_f = 0.80 (5% MeOH/EA); ¹H NMR (500 MHz, CDCl₃): δ 8.06 (s, 1H, H-8), 8.02 (s, 1H, H-2), 5.49 (t, J = 7.3 Hz, 1H, CH=), 5.07–5.03 (m, 1H, CH=), 5.01 (d, J = 7.3 Hz, 2H, NCH₂), 2.12 (br s, 4H, 2 × CH₂), 1.83 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.57 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 154.3 (C, C-6), 153.9 (CH, C-8), 150.9 (C, C-4), 145.0 (C=), 141.7 (CH, C-2), 132.5 (C=), 123.4 (CH=), 120.8 (C, C-5), 116.2 (CH=), 47.4 (NCH₂), 39.6 (CH₂), 26.2 (CH₂), 25.8 (CH₃), 17.9 (CH₃), 16.8 (CH₃); IR (film from CH₂Cl₂): ν_max 3231, 3067, 2966, 2912, 2853 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₅H₂₂N₅ 272.1870; Found 272.1875 (Δ = 1.8 ppm); HRESIMS/MS (40 eV) m/z (%): 136.0612 (100), 81.0700 (17).

Compound 5: 7.8 mg (3%), pale-yellow oil; R_f = 0.04 (10% MeOH/EA); ¹H NMR (600 MHz, CDCl₃): δ 7.68 (s, 1H, H-8), 7.26 (s, 1H, H-2), 5.47 (t, J = 7.3 Hz, 1H, CH=), 5.35 (t, J = 7.6 Hz, 1H, CH=), 5.15 (d, J = 7.3 Hz, 2H, NCH₂), 5.08–5.00 (m, 2H, 2 × CH=), 4.60 (d, J = 7.1 Hz, 2H, NCH₂), 2.15–2.03 (m, 8H, 4 × CH₂), 1.78 (s, 3H, CH₃), 1.77 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.65 (s, 3H, CH₃), 1.58 (s, 3H, CH₃), 1.57 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.2 (C, C-6), 144.9 (CH, C-8), 144.6 (C, C-4), 143.5 (C=), 143.0 (C=), 140.5 (CH, C-2), 132.43 (C=), 132.38 (C=), 123.7 (CH=), 123.4 (CH=), 117.0 (CH=), 116.4 (CH=), 112.6 (C, C-5), 45.7 (NCH₂), 46.1 (NCH₂), 39.63 (CH₂), 39.60 (CH₂), 26.23 (CH₂), 26.22 (CH₂), 25.9 (CH₃), 25.8 (CH₃), 17.88 (CH₃), 17.85 (CH₃), 16.79 (CH₃), 16.76 (CH₃); IR (film from CH₂Cl₂): ν_max 2966, 2916, 2855, 1629 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₅H₃₈N₅ 408.3122; Found 408.3129 (Δ = 1.7 ppm); HRESIMS/MS (20 eV) m/z (%): 272.1851 (41), 136.0608 (100), 81.0698 (20).

3.2.2. 3-((6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-yl)-3H-purin-6-amine (6) and 9-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-9H-purin-6-amine (7)

Adenine (0.51 mmol, 68.3 mg), K₂CO₃ (0.52 mmol, 71.9 mg) and farnesyl bromide (0.55 mmol, 157.0 mg) in DMF (2 mL) at 50 °C for 27 h yielded 6 and 7, with modified work up-H₂O (6 mL) was added to the reaction filtrate and stored in the fridge until precipitate formed. The isolated solid was purified by chromatography.

Compound 6: 22.4 mg (13%), pale-yellow crystals; R_f = 0.17 (5% MeOH/EA); 3:2 E/Z, data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 8.05 (s, 1H, H-8), 8.00 (s, 1H, H-2), 5.51–5.45 (m, 1H, CH=), 5.10–5.03 (m, 2H, 2 × CH=), 5.01 (d, J = 7.3 Hz, 2H, NCH₂), 2.15–2.09 (m, 4H, 2 × CH₂), 2.05–1.98 (m, 2H, CH₂), 1.98–1.92 (m, 2H, CH₂), 1.83 (s, 3H, CH₃), 1.65 (s, 3H, CH₃), 1.57 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 154.5 (C, C-6), 154.1 (CH, C-8), 150.9 (C, C-4), 144.9 (C=), 141.6 (CH, C-2), 136.1 (C=), 131.5 (C=), 124.3 (CH=), 123.3 (CH=), 121.1 (C, C-5), 116.2 (CH=), 47.4 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 26.8 (CH₂), 26.2 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.9 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3231, 3065, 2965, 2915, 2855, 1704 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₀H₃₀N₅ 340.2496; Found 340.2505 (Δ = 2.6 ppm); HRESIMS/MS (40 eV) m/z (%): 136.0610 (100), 119.0345 (13).

Compound 7: 3.6 mg (2%), white powder; R_f = 0.30 (EA); 3:2 E/Z, NMR data for major isomer: ¹H NMR (500 MHz, CDCl₃): δ 8.38 (s, 1H, H-2), 7.78 (s, 1H, H-8), 5.59 (br s, 2H, NH₂), 5.45 (t, J = 7.1 Hz, 1H, CH=), 5.13–5.03 (m, 2H, 2 × CH=), 4.78 (d, J = 7.1 Hz, 2H, NCH₂), 2.19–2.08 (m, 4H, 2 × CH₂), 2.08–1.92 (m, 4H, 2 × CH₂), 1.81 (s, 3H, CH₃), 1.67 (s, 6H, 2 × CH₃), 1.59 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.4 (C, C-6), 153.0 (CH, C-2), 150.1 (C, C-4), 143.0 (C=), 140.2 (CH, C-8), 135.9 (C=), 131.6 (C=), 124.3 (CH=), 123.5 (CH=), 119.7 (C, C-5), 117.5 (CH=), 41.4 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 26.8 (CH₂), 26.3 (CH₂), 25.9 (CH₂), 17.9 (CH₃), 16.7 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3307, 3140, 2965, 2924, 2865, 1601 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₀H₃₀N₅ 340.2496; Found 340.2501 (Δ = 1.5 ppm); HRESIMS/MS (40 eV) m/z (%): 136.0613 (100), 119.0346 (12).

3.2.3. 9-((2E,6E,10E)-3,7,11,15-Tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-9H-purin-6-amine (8)

Adenine (0.69 mmol, 93.8 mg), K₂CO₃ (0.78 mmol, 108.0 mg) and geranylgeranyl bromide (0.73 mmol, 259 mg) in DMF (1 mL) for 27 h yielded 8, 9.9 mg (4%), pale-yellow crystals. R_f = 0.32 (EA); ¹H NMR (500 MHz, CDCl₃): δ 8.37 (s, 1H, H-2), 7.77 (s, 1H, H-8), 5.77 (br s, 2H, NH₂), 5.44 (t, J = 7.2 Hz, 1H, CH=), 5.11–5.05 (m, 3H, 3 × CH=), 4.77 (d, J = 7.2 Hz, 2H, NCH₂), 2.16–2.08 (m, 4H, 2 × CH₂), 2.08–2.01 (m, 4H, 2 × CH₂), 2.01–1.92 (m, 4H, 2 × CH₂), 1.81 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.59 (s, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.5 (C, C-6), 153.0 (CH, C-2), 150.1 (C, C-4), 143.0 (C=), 140.1 (CH, C-8), 135.9 (C=), 135.2 (C=), 131.4 (C=), 124.5 (CH=), 124.2 (CH=), 123.5 (CH=), 119.7 (C, C-5), 117.5 (CH=), 41.3 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 39.6 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃), 16.1 (CH₃); IR (film from CH₂Cl₂): ν_max 3468, 3324, 3153, 3051, 2969 2918 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₅H₃₈N₅ 408.3122; Found 408.3129 (Δ = 1.7 ppm); HRESIMS/MS (40 eV) m/z (%): 136.0614 (100).

3.2.4. (E)-9-(3,7-Dimethylocta-2,6-dien-1-yl)-N,N-dimethyl-9H-purin-6-amine (9) and (E)-3-(3,7-dimethylocta-2,6-dien-1-yl)-N,N-dimethyl-3H-purin-6-amine (10)

6-(Dimethylamino)purine (0.17 mmol, 28.3 mg), Na₂CO₃/K₂CO₃ (1:1, 108 mg) and geranyl bromide (0.42 mmol, 90.8 mg) in DMF (3 mL) for 48 h yielded 9 and 10, with modified work up–concentration under reduced pressure.

Compound 9: 9.2 mg (18%), white powder; R_f = 0.12 (1:2 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 8.35 (s, 1H, H-2), 7.69 (s, 1H, H-8), 5.43 (t, J = 7.1 Hz, 1H, CH=), 5.05 (t, J = 6.1 Hz, 1H, CH=), 4.75 (d, J = 7.1 Hz, 2H, NCH₂), 3.53 (br s, 6H, 2 × NCH₃), 2.15–2.04 (m, 4H, 2 × CH₂) 1.79 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.58 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.1 (C, C-6), 152.5 (CH, C-2), 150.5 (C, C-4), 142.5 (C=), 137.9 (CH, C-8), 132.2 (C=), 123.7 (CH=), 120.3 (C, C-5), 117.9 (CH=), 41.1 (NCH₂), 39.6 (CH₂), 38.7 (2 × NCH₃), 26.3 (CH₂), 25.8 (CH₃), 17.9 (CH₃), 16.6 (CH₃); IR (film from CH₂Cl₂): ν_max 2963, 2919, 1637 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₇H₂₆N₅ 300.2183; Found 300.2184 (Δ = 0.3 ppm). HRESIMS/MS (40 eV) m/z (%): 164.0914 (100), 149.0683 (11), 121.0503 (16).

Compound 10: 6.3 mg (12%), white powder; R_f = 0.21 (5% MeOH/EA); ¹H NMR (600 MHz, CDCl₃): δ 8.00 (s, 1H, H-8), 7.95 (s, 1H, H-2), 5.48 (t, J = 6.9 Hz, 1H, CH=), 5.07–5.02 (m, 1H, CH=), 4.95 (d, J = 7.1 Hz, 2H, NCH₂), 3.92 (br s, 3H, NCH₃), 3.34 (br s, 3H, NCH₃), 2.17–2.08 (m, 4H, 2 × CH₂), 1.81 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.58 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 153.4 (C, C-6), 152.6 (C, C-8), 150.7 (C, C-4), 144.4 (C=), 140.3 (CH, C-2), 132.4 (C=), 123.6 (CH=), 121.6 (C, C-5), 116.6 (CH=), 46.9 (NCH₂), 39.9 (NCH₃), 39.6 (CH₂), 38.1 (NCH₃), 26.3 (CH₂), 25.8 (CH₃), 17.9 (CH₃), 16.7 (CH₃); IR (film from CH₂Cl₂): ν_max 3077, 2964, 2922, 1607 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₇H₂₆N₅ 300.2183; Found 300.2184 (Δ = 0.3 ppm); HRESIMS/MS (20 eV) m/z (%): 164.0914 (100).

3.2.5. N,N-Dimethyl-9-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-9H-purin-6-amine (11) and N,N-dimethyl-3-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-3H-purin-6-amine (12)

6-(Dimethylamino)purine (0.088 mmol, 14.4 mg), K₂CO₃ (0.16 mmol, 22.3 mg) and farnesyl bromide (0.11 mmol, 31.6 mg) in DMF (1 mL) at 50 °C for 21 h yielded 11 and 12.

Compound 11: 5.6 mg (17%), white powder; R_f = 0.29 (1:1 EA/PE); 3:2 E/Z, NMR data for major isomer: ¹H NMR (500 MHz, CDCl₃): δ 8.36 (s, 1H, H-2), 7.70 (s, 1H, H-8), 5.44 (t, J = 7.1 Hz, 1H, CH=), 5.11–5.03 (m, 2H, 2 × CH=), 4.75 (d, J = 7.1 Hz, 2H, NCH₂), 3.53 (br s, 6H, 2 × NCH₃), 2.16–2.06 (m, 4H, 2 × CH₂), 2.06–1.99 (m, 2H, CH₂), 1.99–1.92 (m, 2H, CH₂), 1.81 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.58 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.1 (C, C-6), 152.5 (CH, C-2), 150.5 (C, C-4), 142.5 (C=), 137.9 (CH, C-8), 135.9 (C=), 131.5 (C=), 124.4 (CH=), 120.3 (C, C-5), 117.9 (CH=), 41.2 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 38.7 (2 × NCH₃), 26.8 (CH₂), 26.3 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3051, 2961, 2917, 2856, 1589 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₂H₃₄N₅ 368.2809; Found 368.2817 (Δ = 2.2 ppm); HRESIMS/MS (20 eV) m/z (%): 164.0894 (100).

Compound 12: 9.5 mg (29%), white powder; R_f = 0.25 (10% MeOH/EA); 3:2 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 8.01 (s, 1H, H-8), 7.95 (s, 1H, H-2), 5.48 (t, J = 7.3 Hz, 1H, CH=), 5.09–5.03 (m, 2H, 2 × CH=), 4.96 (d, J = 7.3 Hz, 2H, NCH₂), 3.92 (br s, 3H, NCH₃), 3.33 (br s, 3H, NCH₃), 2.17–2.08 (m, 4H, 2 × CH₂), 2.05–1.99 (m, 2H, CH₂), 1.99–1.94 (m, 2H, CH₂), 1.82 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.58 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 153.4 (C, C-6), 152.4 (CH, C-8), 150.5 (C, C-4), 144.5 (C=), 140.4 (CH, C-2), 136.1 (C=), 131.5 (C=), 124.4 (CH=), 123.3 (CH=), 121.6 (C, C-5), 116.5 (CH=), 46.9 (NCH₂), 39.9 (NCH₃), 39.8 (CH₂), 39.6 (CH₂), 38.1 (NCH₃), 26.8 (CH₂), 26.2 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.8 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 2963, 2924, 2856, 1608 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₂H₃₄N₅ 368.2809; Found 368.2818 (Δ = 2.4 ppm); HRESIMS/MS (40 eV) m/z (%): 164.0932 (100), 81.0704 (14).

3.2.6. N,N-Dimethyl-9-((2Z,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-9H-purin-6-amine (13) and N,N-dimethyl-9-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-9H-purin-6-amine (14)

6-(Dimethylamino)purine (0.14 mmol, 23.4 mg), K₂CO₃ (0.16 mmol, 22.7 mg) and geranylgeranyl bromide (0.15 mmol, 51.5 mg) in DMF (1 mL) for 44 h yielded 13 and 14.

Compound 13: 4.5 mg (28%), white solid; R_f = 0.21 (1:2 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 8.35 (s, 1H, H-2), 7.70 (s, 1H, H-8), 5.43 (t, J = 7.1 Hz, 1H, CH=), 5.15–5.04 (m, 3H, 3 × CH=), 4.75 (d, J = 7.1 Hz, 2H, NCH₂), 3.53 (br s, 6H, 2 × NCH₃), 2.27–2.20 (m, 2H, CH₂), 2.18–2.10 (m, 2H, CH₂), 2.10–2.01 (m, 4H, 2 × CH₂), 2.01–1.91 (m, 4H, 2 × CH₂), 1.79 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.61 (s, 3H, CH₃), 1.59 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.1 (C, C-6), 152.5 (CH, C-2), 150.5 (C, C-4), 142.4 (C=), 137.9 (CH, C-8), 136.3 (C=), 135.2 (C=), 131.4 (C=), 124.5 (CH=), 124.2 (CH=), 123.3 (CH=), 120.7 (C, C-5), 118.7 (CH=), 41.0 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 38.6 (2 × NCH₃), 32.3 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.5 (CH₂), 25.9 (CH₃), 23.6 (CH₃), 17.8 (CH₃), 16.20 (CH₃), 16.16 (CH₃); IR (film from CH₂Cl₂): ν_max 3043, 2921, 2854, 1590 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₇H₄₂N₅ 436.3435; Found 436.3430 (Δ = −1.1 ppm); HRESIMS/MS (40 eV) m/z (%): 164.0912 (100).

Compound 14: 6.7 mg (14%), white solid; R_f = 0.18 (1:2 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 8.36 (s, 1H, H-2), 7.70 (s, 1H, H-8), 5.43 (t, J = 7.0 Hz, 1H, CH=), 5.12–5.04 (m, 3H, 3 × CH=), 4.75 (d, J = 7.1 Hz, 2H, NCH₂), 3.56 (br s, 6H, 2 × NCH₃), 2.15–2.08 (m, 4H, 2 × CH₂), 2.08–2.01 (m, 4H, 2 × CH₂), 1.99–1.93 (m, 4H, 2 × CH₂), 1.80 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.59 (s, 3H, CH₃), 1.58 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.1 (C, C-6), 152.5 (CH, C-2), 150.5 (C, C-4), 142.5 (C=), 137.9 (CH, C-8), 135.9 (C=), 135.2 (C=), 131.4 (C=), 124.5 (CH=), 124.2 (CH=), 123.5 (CH=), 120.3 (C, C-5), 117.9 (CH=), 41.1 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 39.6 (CH₂), 38.6 (2 × NCH₃), 26.9 (CH₂), 26.7 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃), 16.1 (CH₃); IR (film from CH₂Cl₂): ν_max 3104, 2962, 2917, 2855, 1590 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₇H₄₂N₅ 436.3435; Found 436.3466 (Δ = 7.1 ppm); HRESIMS/MS (40 eV) m/z (%): 164.0873 (100).

3.2.7. (E)-6-Chloro-9-(3,7-dimethylocta-2,6-dien-1-yl)-9H-purine (15) and (E)-6-chloro-7-(3,7-dimethylocta-2,6-dien-1-yl)-7H-purine (16)

Following the general alkylation procedure, also previously published using 80 °C [54], 6-chloropurine (0.30 mmol, 45.9 mg), K₂CO₃ (0.62 mmol, 85.6 mg) and geranyl bromide (0.33 mmol, 71.8 mg) in DMF (1 mL) for 21 h yielded 15 and 16.

Compound 15: 39.4 mg (46%), colourless oil; R_f = 0.48 (2:3 EA/PE); IR data and select ¹H NMR data previously reported in CD₃OD [54]; ¹H NMR (300 MHz, CDCl₃): δ 8.72 (s, 1H, H-2), 8.07 (s, 1H, H-8), 5.42 (t, J = 7.2 Hz, 1H, CH=), 5.05–4.97 (m, 1H, CH=), 4.84 (d, J = 7.3 Hz, 2H, NCH₂), 2.09 (s, 4H, 2 × CH₂), 1.81 (s, 3H, CH₃), 1.64 (s, 3H, CH₃), 1.55 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 151.9 (CH, C-2), 151.8 (C, C-6), 150.9 (C, C-4), 144.8 (CH, C-8), 144.1 (C=), 132.4 (C, C-5), 131.8 (C=), 123.4 (CH=), 116.7 (CH=), 41.9 (NCH₂), 39.5 (CH₂), 26.1 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.7 (CH₃); HRESIMS m/z: [M + H]⁺ Calcd. for C₁₅H₂₀ClN₄ 291.1371; Found 291.1371 (Δ = 0.0 ppm); HRESIMS/MS (40 eV) m/z (%): 157.0074 (22), 155.0105 (57), 119.0346 (100).

Compound 16: 17.2 mg (20%), colourless oil; R_f = 0.16 (2:3 EA/PE); IR data and select ¹H NMR data previously reported in CD₃OD [54]; ¹H NMR (300 MHz, CDCl₃): δ 8.85 (s, 1H, H-2), 8.23 (s, 1H, H-8), 5.43 (t, J = 6.8 Hz, 1H, CH=), 5.07 (d, J = 7.0 Hz, 2H, NCH₂), 5.05–5.00 (m, 1H, CH=), 2.13 (s, 4H, 2 × CH₂), 1.80 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.58 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 162.2 (C, C-6), 152.5 (CH, C-2), 148.4 (CH, C-8), 144.2 (C=), 143.3 (C, C-4), 132.6 (C=), 123.3 (CH=), 122.7 (C, C-5), 117.1 (CH=), 45.4 (NCH₂), 39.5 (CH₂), 26.1 (CH₂), 25.8 (CH₃), 17.9 (CH₃), 16.8 (CH₃); HRESIMS m/z: [M + H]⁺ Calcd. for C₁₅H₂₀ClN₄ 291.1371; Found 291.1365 (Δ = −2.1 ppm); HRESIMS/MS (40 eV) m/z (%): 157.0062 (100), 155.0081 (40).

3.2.8. 6-Chloro-9-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-9H-purine (17) and 6-chloro-7-((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-9H-purine (18)

6-Chloropurine (0.31 mmol, 48.5 mg), K₂CO₃ (0.6 mmol, 83 mg) and farnesyl bromide (0.33 mmol, 94.0 mg) in DMF (2 mL) at 50 °C for 24 h yielded 17 and 18.

Compound 17: 47.4 mg (43%), colourless oil; R_f = 0.24 (1:2 EA/PE); 2:1 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 8.72 (s, 1H, H-2), 8.08 (s, 1H, H-8), 5.42 (t, J = 7.2 Hz, 1H, CH=), 5.06–5.00 (m, 2H, 2 × CH=), 4.85 (d, J = 7.3 Hz, 2H, NCH₂), 2.13–2.07 (m, 4H, 2 × CH₂), 2.02–1.96 (m, 2H, CH₂), 1.95–1.90 (m, 2H, CH₂), 1.82 (s, 3H, CH₃), 1.63 (s, 3H, CH₃), 1.55 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 152.0 (C, C-2), 151.8 (C, C-6), 151.0 (C, C-4), 144.9 (C, C-8), 144.1 (C=), 136.0 (C=), 132.3 (C, C-5), 131.5 (C=), 124.3 (CH=), 123.3 (CH=), 116.6 (CH=), 41.9 (NCH₂), 39.8 (CH₂), 39.5 (CH₂), 26.8 (CH₂), 26.2 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.8 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3115, 2969, 2930, 1335, 939, 637 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₀H₂₈ClN₄ 359.1997; Found 359.1993 (Δ = −1.1 ppm); HRESIMS/MS (40 eV) m/z (%): 157.0073 (40), 155.0103 (100), 119.0343 (47), 81.0697 (47).

Compound 18: 21.3 mg (19%), colourless oil; R_f = 0.20 (1:1 EA/PE); E/Z 3:2, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 8.86 (s, 1H, H-2), 8.23 (s, 1H, H-8), 5.44 (t, J = 7.0 Hz, 1H, CH=), 5.08 (d, J = 7.1 Hz, 2H, NCH₂), 5.07–5.04 (m, 2H, 2 × CH=), 2.18–2.09 (m, 4H, 2 × CH₂), 2.05–1.99 (m, 2H, CH₂), 1.98–1.93 (m, 2H, CH₂), 1.82 (s, 3H, CH₃), 1.65 (s, 3H, CH₃), 1.58 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 162.0 (C, C-6), 152.4 (CH, C-2), 148.5 (CH, C-8), 144.2 (C=), 143.3 (C, C-4), 136.2 (C=), 131.5 (C=), 124.2 (CH=), 123.2 (CH=), 122.6 (C, C-5), 117.0 (CH=), 45.4 (NCH₂), 39.8 (CH₂), 39.5 (CH₂), 26.8 (CH₂), 26.2 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.9 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3053, 2981, 1264, 732 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₀H₂₈ClN₄ 359.1997; Found 359.2002 (Δ = 1.4 ppm); HRESIMS/MS (40 eV) m/z (%): 157.0001 (27), 155.0031 (66), 119.0287 (31), 95.0806 (23), 93.0651 (13), 81.0660 (100), 79.0539 (15).

3.2.9. 6-Chloro-9-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-9H-purine (19), 6-chloro-7-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-7H-purine (20), and 6-chloro-7-((2Z,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-7H-purine (21)

Following the general alkylation procedure, also previously published [55], 6-chloropurine (0.30 mmol, 45.6 mg), K₂CO₃ (0.35 mmol, 48.2 mg) and geranylgeranyl bromide (0.32 mmol, 111 mg) in DMF (1 mL) for 27 h yielded 19, 20, and 21_.

Compound 19: 19.4 mg (19%), colourless oil; R_f = 0.51 (2:3 EA/PE); ¹H and ¹³C NMR data previously reported [55]; IR (film from CH₂Cl₂): ν_max 3070, 2966, 2922, 2855, 1592, 1560, 1335 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₅H₃₆ClN₄ 427.2623; Found 427.2614 (Δ = −2.1 ppm); HRESIMS/MS (40 eV) m/z (%): 157.0068 (39), 155.0098 (100), 119.0334 (21).

Compound 20: 15.2 mg (15%), colourless oil; R_f = 0.21 (2:3 EA/PE); ¹H and ¹³C NMR data previously reported [55]; IR (film from CH₂Cl₂): ν_max 3053, 2971, 2931, 733 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₅H₃₆ClN₄ 427.2623; Found 427.2642 (Δ = 4.4 ppm).

Compound 21: 16.8 mg (49%), colourless oil; R_f = 0.3 (2:3 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 8.87 (s, 1H, H-2), 8.23 (s, 1H, H-8), 5.45 (t, J = 7.1 Hz, 1H, CH=), 5.15–5.08 (m, 3H, 3 × CH=), 5.07 (d, J = 7.1 Hz, 2H, NCH₂), 2.28–2.21 (m, 2H, CH₂), 2.21–2.13 (m, 2H, CH₂), 2.10–2.02 (m, 4H, 2 × CH₂), 2.02–1.93 (m, 4H, 2 × CH₂), 1.84 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.62 (s, 3H, CH₃), 1.59 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 162.1 (C, C-6), 152.5 (CH, C-2), 148.4 (CH, C-8), 144.1 (C=), 143.2 (C, C-4), 136.9 (C=), 135.4 (C=), 131.3 (C=), 124.4 (CH=), 124.1 (CH=), 122.9 (CH=), 122.5 (C, C-5), 117.9 (CH=), 45.2 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 32.5 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 23.6 (CH₃), 17.8 (CH₃), 16.18 (CH₃), 16.16 (CH₃); IR (film from CH₂Cl₂): ν_max 3055, 2973, 2932, 733 cm⁻¹_; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₅H₃₆ClN₄ 427.2623; Found 427.2634 (Δ = 2.6 ppm); HRESIMS/MS (40 eV) m/z (%): 157.0086 (32), 155.0118 (100), 121.10185 (10), 119.03516 (16), 109.1013 (11), 107.0858 (15).

3.2.10. 3,7-Bis((E)-3,7-dimethylocta-2,6-dien-1-yl)-3,7-dihydro-1H-purine-2,6-dione (27)

Xanthine (2.1 mmol, 311.5 mg), K₂CO₃ (2.0 mmol, 272.2 mg) and geranyl bromide (2.4 mmol, 521 mg) in DMF (3 mL) for 48 h yielded 27, with modified work up—concentrating under reduced pressure, 44.6 mg (9%), colourless oil. R_f = 0.15 (1:2 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 8.10 (br s, 1H, NH), 7.55 (s, J = 2.8 Hz, 1H, H-8), 5.43 (t, J = 6.7 Hz, 1H, CH=), 5.34 (t, J = 6.4 Hz, 1H, CH=), 5.07–5.01 (m, 2H, 2 × CH=), 4.89 (d, J = 7.3 Hz, 2H, NCH₂), 4.66 (d, J = 6.9 Hz, 2H, NCH₂), 2.15–2.09 (m, 4H, 2 × CH₂), 2.09–2.03 (m, 2H, CH₂), 2.02–1.96 (m, 2H, CH₂), 1.84 (s, 3H, CH₃), 1.77 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.63 (s, 3H, CH₃), 1.59 (s, 3H, CH₃), 1.56 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 154.7 (C, C-6), 150.62 (C, C-2 or C-4), 150.58 (C, C-2 or C-4), 143.8 (C=), 140.8 (CH=), 140.7 (CH, C-8), 132.4 (C=), 131.8 (C=), 124.0 (CH=), 123.6 (CH=), 118.0 (CH=), 117.2 (CH=), 107.5 (C, C-5), 44.7 (NCH₂), 40.9 (NCH₂), 39.7 (CH₂), 39.6 (CH₂), 26.5 (CH₂), 26.3 (CH₂), 25.84 (CH₃), 25.80 (CH₃), 17.9 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.6 (CH₃); IR (film from CH₂Cl₂): ν_max 3423, 3176, 3115, 3052, 2967, 2926, 1679 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₅H₃₇N₄O₂ 425.2911; Found 425.2910 (Δ = −0.2 ppm); HRESIMS/MS (10 eV) m/z (%): 289.1638 (70), 153.0395 (100).

3.2.11. 3,7-Bis((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-3,7-dihydro-1H-purine-2,6-dione (28)

Xanthine (2.0 mmol, 306.3 mg), K₂CO₃ (3.0 mmol, 420.3 mg) and farnesyl bromide (2.4 mmol, 683.4 mg) in DMF (3 mL) for 25 h yielded 28, 19.3 mg (3%), colourless oil. R_f = 0.25 (1:2 EA/PE); 3:2 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 8.57 (br s, 1H, NH), 7.54 (s, 1H, H-8), 5.43 (t, J = 6.6 Hz, 1H, CH=), 5.34 (t, J = 6.8 Hz, 1H, CH=), 5.12–5.02 (m, 4H, 4 × CH=), 4.89 (d, J = 7.2 Hz, 2H, NCH₂), 4.66 (d, J = 6.9 Hz, 2H, NCH₂), 2.16–1.89 (m, 16H, 8 × CH₂), 1.85 (s, 3H, CH₃), 1.78 (s, 3H, CH₃), 1.66 (s, 6H, 2 × CH₃), 1.58 (s, 9H, 3 × CH₃), 1.55 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 154.9 (C, C-6), 150.7 (C, C-2 or C-4), 150.6 (C, C-2 or C-4), 143.8 (C=), 141.1 (C=), 140.8 (CH, C-8), 136.0 (C=), 135.4 (C=), 131.5 (C=), 131.4 (C=), 124.4 (CH=), 124.3 (CH=), 123.9 (CH=), 123.4 (CH=), 118.0 (CH=), 117.2 (CH=), 107.5 (C, C-5), 44.7 (NCH₂), 40.8 (NCH₂), 39.79 (CH₂), 39.78 (CH₂), 39.7 (CH₂), 39.6 (CH₂), 26.81 (CH₂), 26.78 (CH₂), 26.4 (CH₂), 26.3 (CH₂), 25.84 (CH₃), 25.83 (CH₃), 17.82 (CH₃), 17.81 (CH₃), 16.74 (CH₃), 16.67 (CH₃), 16.2 (CH₃), 16.1 (CH₃); IR (film from CH₂Cl₂): ν_max 3166, 3065, 2964, 2927, 2856, 1686 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₃₅H₅₃N₄O₂ 561.4163; Found 561.4169 (Δ = 1.1 ppm); HRESIMS/MS (20 eV) m/z (%): 561.1128 (39), 357.2262 (48), 153.0391 (100).

3.2.12. (E)-7-(3,7-Dimethylocta-2,6-dien-1-yl)-3-methyl-3,7-dihydro-1H-purine-2,6-dione (29)

3-Methylxanthine (0.17 mmol, 28.0 mg), K₂CO₃/Na₂CO₃ (1:1, 48.6 mg) and geranyl bromide (0.36 mmol, 77 mg) in DMF (2 mL) at 50 °C for 48 h yielded 29, with modified work up—the concentrated reaction was filtered from DCM, and additionally recrystallised from PE after chromatography, 16.9 mg (5%), white powder. R_f = 0.2 (1:2 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 8.10 (s, 1H, NH), 7.55 (s, 1H, H-8), 5.43 (t, J = 7.3 Hz, 1H, CH=), 5.07–5.03 (m, 1H, CH=), 4.90 (d, J = 7.3 Hz, 2H, NCH₂), 3.55 (s, 3H, NCH₃), 2.16–2.08 (m, 4H, 2 × CH₂), 1.78 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.59 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 154.5 (C, C-6), 150.99 (C, C-2), 150.96 (C, C-4), 143.9 (C=), 140.8 (CH, C-8), 132.4 (C=), 123.5 (CH=), 117.1 (CH=), 107.4 (C, C-5), 44.8 (NCH₂), 39.6 (CH₂), 29.2 (NCH₃), 26.2 (CH₂), 25.9 (CH₃), 17.9 (CH₃), 16.6 (CH₃); IR (film from CH₂Cl₂): ν_max 3121, 3021, 2964, 2916, 2826, 1678 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₆H₂₃N₄O₂ 303.1816; Found 303.1812 (Δ = −1.3 ppm); HRESIMS/MS (40 eV) m/z (%): 167.0538 (100), 149.0431 (8), 124.0487 (17).

3.2.13. 3-Methyl-7-((2Z,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-3,7-dihydro-1H-purine-2,6-dione (30) and 3-methyl-7-((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-3,7-dihydro-1H-purine-2,6-dione (31)

3-Methylxanthine (0.20 mmol, 32.9 mg), K₂CO₃ (0.37 mmol, 51.2 mg) and farnesyl bromide (0.34 mmol, 98 mg) in DMF (2 mL) for 21 h yielded 30 and 31.

Compound 30: 4.6 mg (8%), white solid; R_f = 0.20 (1:1 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 8.10 (s, 1H, NH), 7.55 (s, 1H, H-8), 5.44 (t, J = 6.9 Hz, 1H, CH=), 5.11–5.05 (m, 2H, 2 × CH=), 4.88 (d, J = 7.4 Hz, 2H, NCH₂), 3.54 (s, 3H, NCH₃), 2.23–2.17 (m, 2H, CH₂), 2.15–2.10 (m, 2H, CH₂), 2.07–2.01 (m, 2H, CH₂), 1.99–1.93 (m, 2H, CH₂), 1.81 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.60 (s, 3H, CH₃), 1.59 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 154.5 (C, C-6), 151.0 (C, C-2), 150.9 (C, C-4), 143.7 (C=), 140.8 (CH, C-8), 136.5 (C=), 131.6 (C=), 124.3 (CH=), 123.1 (CH=), 118.0 (CH=), 107.4 (C, C-5), 44.6 (NCH₂), 39.8 (CH₂), 32.2 (CH₂), 29.2 (NCH₃), 26.7 (CH₂), 26.4 (CH₂), 25.9 (CH₃), 23.6 (CH₃), 17.8 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3400, 3162, 3035, 2969, 2930, 1683 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₁H₃₁N₄O₂ 371.2442; Found 371.2452 (Δ = 2.7 ppm).

Compound 31: 33.1 mg (45%), white solid; R_f = 0.18 (1:1, EA/PE); 2:1 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 8.89 (s, 1H, NH), 7.56 (s, 1H, H-8), 5.42 (t, J = 7.3 Hz, 1H, CH=), 5.08–5.04 (m, 2H, 2 × CH=), 4.90 (d, J = 7.3 Hz, 2H, NCH₂), 3.54 (s, 3H, NCH₃), 2.16–2.07 (m, 4H, 2 × CH₂), 2.06–1.99 (m, 2H, CH₂), 1.98–1.93 (m, 2H, CH₂), 1.78 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.58 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 154.9 (C, C-6), 151.3 (C, C-2), 150.9 (C, C-4), 143.9 (C=), 140.8 (CH, C-8), 136.0 (C=), 131.5 (C=), 124.3 (CH=), 123.4 (CH=), 117.1 (CH=), 107.5 (C, C-5), 44.7 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 29.2 (NCH₃), 26.8 (CH₂), 26.2 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃); HRESIMS m/z: [M + H]⁺ Calcd. for C₂₁H₃₁N₄O₂ 371.2442; Found 371.2445 (Δ = 0.8 ppm); HRESIMS/MS (40 eV) m/z (%): 167.0552 (100), 124.0506 (87).

3.2.14. 3-Methyl-7-((2Z,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-3,7-dihydro-1H-purine-2,6-dione (32) and 3-methyl-7-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-3,7-dihydro-1H-purine-2,6-dione (33)

3-Methylxanthine (0.35 mmol, 40.9 mg), K₂CO₃ (0.38 mmol, 52.5 mg) and geranylgeranyl bromide (0.37 mmol, 130 mg) in DMF (1 mL) for 44 h yielded 32 and 33.

Compound 32: 10.5 mg (26%), white, waxy solid; R_f = 0.20 (1:1, EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 8.45 (s, 1H, NH), 7.55 (s, 1H, H-8), 5.44 (t, J = 7.1 Hz, 1H, CH=), 5.12–5.05 (m, 3H, 3 × CH=), 4.88 (d, J = 7.2 Hz, 2H, NCH₂), 3.54 (s, 3H, NCH₃), 2.23–2.18 (m, 2H, CH₂), 2.15–2.09 (m, 2H, CH₂), 2.08–2.01 (m, 4H, 2 × CH₂), 2.00–1.93 (m, 4H, 2 × CH₂), 1.80 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.60 (s, 3H, CH₃), 1.59 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 154.6 (C, C-6), 151.1 (C, C-2), 150.9 (C, C-4), 143.7 (C=), 140.8 (CH, C-8), 136.5 (C=), 135.3 (C=), 131.4 (C=), 124.5 (CH=), 124.1 (CH=), 123.1 (CH=), 118.0 (CH=), 107.4 (C, C-5), 44.6 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 32.3 (CH₂), 29.2 (NCH₃), 26.9 (CH₂), 26.7 (CH₂), 26.5 (CH₂), 25.8 (CH₃), 23.6 (CH₃), 17.8 (CH₃), 16.20 (CH₃), 16.15 (CH₃); IR (film from CH₂Cl₂): ν_max 3458, 3159, 2968, 2924, 2852 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₆H₃₉N₄O₂ 439.3068; Found 439.3036 (Δ = −7.2 ppm); HRESIMS/MS (40 eV) m/z (%): 168.0565 (9), 167.0533 (100), 124.0494 (43).

Compound 33: 22.1 mg (18%), white waxy solid; R_f = 0.12 (1:1 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 8.94 (s, 1H, NH), 7.56 (s, 1H, H-8), 5.43 (t, J = 7.3 Hz, 1H, CH=), 5.12–5.04 (m, 3H, 3 × CH=), 4.90 (d, J = 7.3 Hz, 2H, NCH₂), 3.54 (s, 3H, NCH₃), 2.16–2.08 (m, 4H, 2 × CH₂), 2.07–2.00 (m, 4H, 2 × CH₂), 2.00–1.93 (m, 4H, 2 × CH₂), 1.78 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.59 (s, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 154.9 (C, C-6), 151.3 (C-2), 150.9 (C-4), 143.9 (C=), 140.8 (CH, C-8), 136.0 (C=), 135.2 (C=), 131.41 (C=), 124.5 (CH=), 124.2 (CH=), 123.4 (CH=), 117.1 (CH=), 107.5 (C, C-5), 44.8 (NCH₂), 39.84 (CH₂), 39.79 (CH₂), 39.6 (CH₂), 29.2 (NCH₃), 26.9 (CH₂), 26.7 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃), 16.1 (CH₃); IR (film from CH₂Cl₂): ν_max 3158, 3121, 3029, 2965, 2924, 2834, 1713, 1679 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₆H₃₉N₄O₂ 439.3068; Found 439.3035 (Δ = −7.5 ppm); HRESIMS/MS (40 eV) m/z (%): 168.0459 (9), 167.0437 (100), 124.0412 (39).

3.2.15. (E)-7-(3,7-Dimethylocta-2,6-dien-1-yl)-1,3-dimethyl-3,7-dihydro-1H-purine-2,6-dione (34)

Theophylline hydrate (2.0 mmol, 391.3 mg), K₂CO₃/Na₂CO₃ (1:1, 244 mg) and geranyl bromide (4.0 mmol, 869 mg) in DMF (3 mL) for 3 h yielded 34, with modified work up and modified purification—H₂O (9 mL) was added to the reaction and the resulting precipitate was isolated and recrystallised from PE, 277.5 mg (45%), white crystals. ¹H NMR (600 MHz, CDCl₃): δ 7.53 (s, 1H, H-8), 5.43 (t, J = 7.6 Hz, 1H, CH=), 5.05 (t, J = 6.2 Hz, 1H, CH=), 4.93 (d, J = 7.3 Hz, 2H, NCH₂), 3.59 (s, 3H, N(3)CH₃), 3.42 (s, 3H, N(1)CH₃), 2.15–2.07 (m, 4H, 2 × CH₂), 1.78 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.59 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.5 (C, C-6), 151.9 (C, C-2), 149.0 (C, C-4), 143.5 (C=), 140.3 (CH, C-8), 132.4 (C=), 123.6 (CH=), 117.5 (CH=), 107.2 (C, C-5), 44.7 (NCH₂), 39.6 (CH₂), 29.9 (N(3)CH₃), 28.1 (N(1)CH₃), 26.3 (CH₂), 25.9 (CH₃), 17.9 (CH₃), 16.6 (CH₃); IR (neat): ν_max 3098, 2964, 2926, 2855, 1695, 1645 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₇H₂₅N₄O₂ 317.1972; Found 317.1978 (Δ = 1.9 ppm); HRESIMS/MS (40 eV) m/z (%): 181.0714 (52), 124.0511 (100).

3.2.16. 1,3-Dimethyl-7-((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-3,7-dihydro-1H-purine-2,6-dione (35)

Theophylline hydrate (1.85 mmol, 370 mg), K₂CO₃/Na₂CO₃ (1:1, 170 mg) and farnesyl bromide (2.0 mmol, 570.5 mg). in DMF (2 mL) at 80 °C for 5 h yielded 35, after modified work up and modified purification as per 34, 333.1 mg (72%), white crystals. ¹H NMR (600 MHz, CDCl₃): δ 7.53 (s, 1H, H-8), 5.43 (t, J = 7.3 Hz, 1H, CH=), 5.09–5.05 (m, 2H, 2 × CH=), 4.93 (d, J = 7.2 Hz, 2H, NCH₂), 3.58 (s, 3H, N(3)CH₃), 3.41 (s, 3H, N(1)CH₃), 2.16–2.07 (m, 4H, 2 × CH₂), 2.03 (m, 2H, CH₂), 1.99–1.93 (m, 2H, CH₂), 1.79 (s, 3H, CH₃), 1.67 (s, 1H, CH₃), 1.60 (s, 3H, CH₃), 1.59 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.5 (C, C-6), 151.9 (C, C-2), 149.0 (C, C-4), 143.5 (C=), 140.3 (CH, C-8), 136.0 (C=), 131.6 (C=), 124.3 (CH=), 123.4 (CH=), 117.5 (CH=), 107.2 (C, C-5), 44.7 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 29.9 (N(3)CH₃), 28.1 (N(1)CH₃), 26.8 (CH₂), 26.3 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3097, 2695, 2922, 1695, 1646 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₂H₃₃N₄O₂ 385.2598; Found 385.2604 (Δ = 1.6 ppm); HRESIMS/MS (40 eV) m/z (%):181.0718 (100), 124.0506 (73).

3.2.17. 1,3-Dimethyl-7-((2Z,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-3,7-dihydro-1H-purine-2,6-dione (36) and 1,3-dimethyl-7-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-3,7-dihydro-1H-purine-2,6-dione (37)

Theophylline hydrate (0.30 mmol, 59.1 mg), K₂CO₃ (0.34 mmol, 46.5 mg) and geranylgeranyl bromide (0.32 mmol, 111 mg) in DMF (1 mL) for 25 h yielded 36 and 37.

Compound 36: 13.1 mg (36%), white solid; R_f = 0.16 (1:2 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 7.52 (s, 1H, H-8), 5.44 (t, J = 7.1 Hz, 1H, CH=), 5.15–5.01 (m, 3H, 3 × CH=), 4.91 (d, J = 7.2 Hz, 2H, NCH₂), 3.58 (s, 3H, N(3)CH₃), 3.41 (s, 3H, N(1)CH₃), 2.24–2.19 (m, 2H, CH₂), 2.15–2.10 (m, 2H, CH₂), 2.08–2.02 (m, 4H, 2 × CH₂), 2.00–1.94 (m, 4H, 2 × CH₂), 1.80 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.60 (s, 3H, CH₃), 1.59 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.4 (C, C-6), 151.9 (C, C-2), 148.9 (C, C-4), 143.3 (C=), 140.3 (CH, C-8), 136.4 (C=), 135.3 (C=), 131.4 (C=), 124.5 (CH=), 124.1 (CH=), 123.1 (CH=), 118.4 (CH=), 107.2 (C, C-5), 44.5 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 32.3 (CH₂), 29.9 (N(3)CH₃), 28.1 (N(1)CH₃), 26.9 (CH₂), 26.7 (CH₂), 26.5 (CH₂), 25.9 (CH₃), 23.6 (CH₃), 17.8 (CH₃), 16.21 (CH₃), 16.15 (CH₃); IR (film from CH₂Cl₂): ν_max 3111, 2917, 2853, 1704, 1658 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₇H₄₁N₄O₂ 453.3224; Found 453.3215 (Δ = −2.0 ppm); HRESIMS/MS (40 eV) m/z (%): 181.0700 (100), 124.0496 (36).

Compound 37: 21.1 mg (19%), white solid; R_f = 0.20 (2:3 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 7.52 (s, 1H, H-8), 5.43 (t, J = 7.1 Hz, 1H, CH=), 5.12–5.04 (m, 3H, 3 × CH=), 4.92 (d, J = 7.2 Hz, 2H, NCH₂), 3.58 (s, 3H, N(3)CH₃), 3.40 (s, 3H, N(1)CH₃), 2.15–2.07 (m, 4H, 2 × CH₂), 2.07–2.01 (m, 4H, 2 × CH₂), 1.99–1.93 (m, 4H, 2 × CH₂), 1.78 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.58 (s, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 155.4 (C, C-6), 151.8 (C, C-2), 148.9 (C, C-4), 143.5 (C=), 140.3 (CH, C-8), 136.0 (C=), 135.2 (C=), 131.4 (C=), 124.5 (CH=), 124.2 (CH=), 123.4 (CH=), 117.5 (CH=), 107.2 (C, C-5), 44.6 (NCH₂), 39.8 (CH₂), 39.8 (CH₂), 39.6 (CH₂), 29.9 (N(3)CH₃), 28.1 (N(1)CH₃), 26.9 (CH₂), 26.7 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃), 16.1 (CH₃); IR (film from CH₂Cl₂): ν_max 3110, 2916, 2854, 1704, 1658 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₇H₄₁N₄O₂ 453.3224; Found 453.3205 (Δ = −4.2 ppm); HRESIMS/MS (40 eV) m/z (%): 181.0693 (100), 124.0489 (31).

3.2.18. (E)-7-(3,7-Dimethylocta-2,6-dien-1-yl)-3-methyl-2-thioxo-1,2,3,7-tetrahydro-6H-purin-6-one (38)

2-Mercapto-3-methylhypoxanthine (0.30 mmol, 54.7 mg), K₂CO₃ (0.61 mmol, 84.3 mg) and geranyl bromide (0.39 mmol, 84.9 mg) in DMF (2 mL) at 70 °C for 30 h yielded 30, after modified work up and modified purification-H₂O (7 mL) was added to the reaction and the resulting isolated precipitate was dissolved in in MeOH and DCM (1:1, 5 mL). Partial evaporation yielded a precipitate isolated by filtration and washed with MeOH, 5.4 mg (6%), off-white powder. ¹H NMR (600 MHz, CDCl₃): δ 9.24 (s, 1H, NH), 7.62 (s, 1H, H-8), 5.43 (t, J = 7.3 Hz, 1H, CH=), 5.07–5.03 (m, 1H, CH=), 4.92 (d, J = 7.3 Hz, 2H, NCH₂), 3.93 (s, 3H, NCH₃), 2.16–2.08 (m, 4H, 2 × CH₂), 1.78 (s, 3H, CH₃), 1.69 (s, 3H, CH₃), 1.60 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 174.3 (C, C-2), 152.4 (C, C-6), 150.9 (C, C-4), 144.5 (C=), 141.2 (CH, C-8), 132.5 (C=), 123.5 (CH=), 116.8 (CH=), 110.9 (C, C-5), 45.0 (NCH₂), 39.6 (CH₂), 35.5 (NCH₃), 26.2 (CH₂), 25.9 (CH₃), 17.9 (CH₃), 16.7 (CH₃); IR (neat): ν_max 3122, 2964, 2912, 2853, 1708 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₆H₂₃N₄OS 319.1587; Found 319.1591 (Δ = 1.3 ppm); HRESIMS/MS (40 eV) m/z (%): 183.033 (48), 149.0452 (19), 126.99579 (11), 124.0503 (100), 96.0557 (21), 81.0702 (46).

3.2.19. 3-Methyl-2-thioxo-7-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-1,2,3,7-tetrahydro-6H-purin-6-one (39)

2-Mercapto-3-methylhypoxanthine (0.32 mmol, 57.8 mg), K₂CO₃ (0.61 mmol, 84.7 mg) and farnesyl bromide (0.39 mmol, 111 mg) in DMF (2 mL) at 50 °C for 41 h yielded 39, 3.3 mg (3%), off-white powder. R_f = 0.19 (1:2 EA/PE); 2:1 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 9.31 (s, 1H, NH), 7.62 (s, 1H, H-8), 5.43 (t, J = 7.3 Hz, 1H, CH=), 5.09–5.05 (m, 2H, 2 × CH=), 4.92 (d, J = 7.3 Hz, 2H, NCH₂), 3.92 (s, 3H, NCH₃), 2.16–2.09 (m, 4H, 2 × CH₂), 2.06–2.01 (m, 4H, 2 × CH₂), 1.78 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.59 (s, 3H, CH₃), 1.58 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 174.3 (C, C-2), 152.4 (C, C-6), 150.9 (C, C-4), 144.5 (C=), 141.1 (CH, C-8), 136.1 (C=), 131.6 (C=), 124.3 (CH=), 123.3 (CH=), 116.8 (CH=), 110.9 (C, C-5), 45.0 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 35.5 (NCH₃), 26.8 (CH₂), 26.2 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3116, 2922, 2854, 1691 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₁H₃₁N₄OS 387.2213; Found 387.2216 (Δ = 0.8 ppm); HRESIMS/MS (40 eV) m/z (%): 183.0316 (100), 149.0436 (13), 124.0493 (42).

3.2.20. 3-Methyl-7-((2Z,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-2-thioxo-1,2,3,7-tetrahydro-6H-purin-6-one (40) and 3-methyl-7-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-2-thioxo-1,2,3,7-tetrahydro-6H-purin-6-one (41)

2-Mercapto-3-methylhypoxanthine (0.30 mmol, 54.7 mg), K₂CO₃ (1.1 mmol, 150.8 mg) and geranylgeranyl bromide (0.32 mmol, 111.3 mg) in DMF (1 mL) for 24 h yielded 40 and 41.

Compound 40: 3.2 mg (9%), white powder; R_f = 0.14 (1:3 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 9.32 (s, 1H, NH), 7.64 (s, 1H, H-8), 5.45 (t, J = 7.2 Hz, 1H, CH=), 5.13–5.07 (m, 3H, 3 × CH=), 4.92 (d, J = 7.3 Hz, 2H, NCH₂), 3.94 (s, 3H, NCH₃), 2.26–2.19 (m, 2H, CH₂), 2.18–2.11 (m, 2H, CH₂), 2.10–2.04 (m, 4H, 2 × CH₂), 2.02–1.96 (m, 4H, 2 × CH₂), 1.83 (s, 3H, CH₃), 1.69 (s, 3H, CH₃), 1.62 (s, 3H, CH₃), 1.61 (s, 3H, CH₃), 1.60 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 174.3 (C, C-2), 152.4 (C, C-6), 150.9 (C, C-4), 144.2 (C=), 141.2 (CH, C-8), 136.6 (C=), 135.3 (C=), 131.5 (C=), 124.5 (CH=), 124.1 (CH=), 123.0 (CH=), 117.7 (CH=), 110.9 (C, C-5), 44.8 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 35.5 (NCH₃), 32.3 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.4 (CH₂), 25.9 (CH₃), 23.6 (CH₃), 17.8 (CH₃), 16.22 (CH₃), 16.16 (CH₃); IR (film from CH₂Cl₂): ν_max 3205, 3118, 2965, 2917, 2855, 1696 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₆H₃₉N₄OS 455.2839; Found 455.2851 (Δ = 2.6 ppm); HRESIMS/MS (40 eV) m/z (%): 183.0304 (100), 124.0487 (19).

Compound 41: 3.4 mg (3%), white powder; R_f = 0.10 (1:3 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 9.29 (s, 1H, NH), 7.62 (s, 1H, H-8), 5.43 (t, J = 7.0 Hz, 1H, CH=), 5.13–5.05 (m, 3H, 3 × CH=), 4.92 (d, J = 7.3 Hz, 2H, NCH₂), 3.92 (s, 3H, NCH₃), 2.17–2.10 (m, 4H, 2 × CH₂), 2.09–2.02 (m, 4H, 2 × CH₂), 2.01–1.93 (m, 4H, 2 × CH₂), 1.79 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.60–1.58 (m, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 174.3 (C, C-2), 152.4 (C, C-6), 150.9 (C, C-4), 144.5 (C=), 141.1 (CH, C-8), 136.1 (C=), 135.2 (C=), 131.5 (C=), 124.5 (CH=), 124.2 (CH=), 123.3 (CH=), 116.8 (CH=), 110.9 (C, C-5), 45.0 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 39.6 (CH₂), 35.5 (NCH₃), 26.9 (CH₂), 26.7 (CH₂), 26.2 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.23 (CH₃), 16.16 (CH₃); IR (film from CH₂Cl₂): ν_max 3212, 3117, 2964, 2917, 2853, 1691 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₆H₃₉N₄OS 455.2839; Found 455.2857 (Δ = 4.0 ppm); HRESIMS/MS (40 eV) m/z (%): 183.0306 (100), 124.0487 (13).

3.2.21. (E)-5-(3,7-Dimethylocta-2,6-dien-1-yl)-1,3-dimethyl-5,7-dihydro-1H-purine-2,6,8(3H)-trione (42)

1,3-Dimethyluric acid (0.12 mmol, 22.6 mg), K₂CO₃ (0.119 mmol, 16.5 mg) and geranyl bromide (0.13 mmol, 27.6 mg) in DMF (2 mL) for 24 h yielded 42, 3.5 mg (9%), white solid. R_f = 0.23 (1:1 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 6.07 (s, 1H, NH), 5.06–5.02 (m, 1H, CH=), 4.95 (t, J = 8.1 Hz, 1H, CH=), 3.49 (s, 3H, N(1)CH₃), 3.28 (s, 3H, N(3)CH₃), 2.67 (d, J = 8.0 Hz, 2H, NCH₂), 2.12–1.99 (m, 4H, 2 × CH₂), 1.70 (s, 3H, CH₃), 1.61 (s, 3H, CH₃), 1.58 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 176.9 (C, C-6), 166.4 (C, C-4), 165.4 (C, C-8), 150.8 (C, C-2), 145.6 (C=), 132.6 (C=), 123.6 (CH=), 112.8 (CH=), 68.1 (C, C-5), 40.6 (NCH₂), 39.9 (CH₂), 32.0 (N(1)CH₃), 29.2 (N(3)CH₃), 26.3 (CH₂), 25.8 (CH₃), 17.9 (CH₃), 16.4 (CH₃); IR (film from CH₂Cl₂): ν_max 3278, 3106, 2966, 2921, 2857, 1750, 1697, 1609 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₇H₂₅N₄O₃ 333.1910; Found 333.1921 (Δ = 3.3 ppm); HRESIMS/MS (40 eV) m/z (%): 197.0664 (95), 169.0712 (100), 140.0463 (32), 112.0505 (41).

3.2.22. 1,3-Dimethyl-5-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-5,7-dihydro-1H-purine-2,6,8(3H)-trione (43) and 1,3-dimethyl-9-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-7,9-dihydro-1H-purine-2,6,8(3H)-trione (44)

1,3-Dimethyluric acid (0.12 mmol, 23.3 mg), K₂CO₃ (0.12 mmol,16.6 mg) and farnesyl bromide (0.11 mmol, 32.0 mg) in DMF (2 mL) for 19 h yielded 43 and 44.

Compound 43: 19.2 mg (40%), white solid; R_f = 0.38 (1:1 EA/PE); 2:1 E/Z NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 6.39 (s, 1H, NH), 5.09–5.02 (m, 2H, 2 × CH=), 4.95 (t, J = 7.9 Hz, 1H, CH=), 3.48 (s, 3H, N(1)CH₃), 3.27 (s, 3H, N(3)CH₃), 2.68 (d, J = 8.1 Hz, 2H, NCH₂), 2.09–2.00 (m, 6H, 3 × CH₂), 2.00–1.93 (m, 2H, CH₂), 1.66 (s, 3H, CH₃), 1.59 (s, 6H, 2 × CH₃), 1.58 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 176.8 (C, C-6), 166.4 (C, C-4), 165.6 (C, C-8), 150.9 (C, C-2), 145.7 (C=), 136.0 (C=), 131.5 (C=), 124.4 (CH=), 123.4 (CH=), 112.7 (CH=), 68.2 (C, C-5), 40.5 (NCH₂), 40.0 (CH₂), 39.8 (CH₂), 32.0 (N(1)CH₃), 29.2 (N(3)CH₃), 26.8 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.4 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3307, 3098, 2964, 2924, 2855, 1695, 1645, 1612 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₂H₃₃N₄O₃ 401.2547; Found 401.2538 (Δ = −2.2 ppm); HRESIMS/MS (40 eV) m/z (%): 197.0639 (100), 169.0699 (40).

Compound 44: 3.3 mg (7%), white solid; R_f = 0.13 (1:1 EA/PE); 3:2 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 8.95 (br s, 1H, NH), 5.16–5.11 (m, 1H, CH=), 5.11–5.00 (m, 2H, 2 × CH=), 4.66 (d, J = 5.8 Hz, 2H, NCH₂), 3.67 (s, 3H, N(3)CH₃), 3.40 (d, J = 2.3 Hz, 3H, N(1)CH₃), 2.13–2.07 (m, 2H, CH₂), 2.07–1.98 (m, 4H, 2 × CH₂), 1.97–1.91 (m, 2H, CH₂), 1.75 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.58 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 153.2 (C, C-6), 151.8 (C, C-8), 151.0 (C, C-2), 140.9 (C=), 136.2 (C=), 136.0 (C, C-4), 131.6 (C=), 124.3 (CH=), 123.3 (CH=), 119.5 (CH=), 98.4 (C, C-5), 41.6 (NCH₂), 39.8 (CH₂), 39.4 (CH₂), 31.3 (N(3)CH₃), 28.6 (N(1)CH₃), 26.8 (CH₂), 26.3 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 17.0 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3487, 3174, 3078, 2918, 2854, 1687, 1651 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₂H₃₃N₄O₃ 401.2547; Found 401.2551 (Δ = 1.0 ppm); HRESIMS/MS (40 eV) m/z (%): 197.0658 (100), 169.0707 (57).

3.2.23. (E)-5-(3,7-Dimethylocta-2,6-dien-1-yl)-5H-pyrazolo[3,4-d]pyrimidin-4-ol (46)

Allopurinol (0.80 mmol, 109.2 mg), K₂CO₃/Na₂CO₃ (1:1, 150.8 mg) and geranyl bromide (0.50 mmol, 108 mg) in DMF (10 mL) for 24 h yielded 46-additionally recrystallised from MeOH after chromatography, 4.5 mg (2%), white powder. R_f = 0.06 (1:4 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 11.32 (br s, 1H, OH), 8.18 (s, 1H, H-3), 8.02 (s, 1H, H-6), 5.34–5.26 (m, 1H, CH=), 5.07–5.02 (m, 1H, CH=), 4.64 (d, J = 7.2 Hz, 2H, NCH₂), 2.16–2.03 (m, 4H, 2 × CH₂), 1.82 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.59 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 157.4 (C, C-4), 153.4 (C, C-7a), 149.4 (CH, C-6), 143.0 (C=), 136.4 (CH, C-3), 132.3 (C=), 123.6 (CH=), 118.1 (CH=), 105.9 (C, C-3a), 43.5 (NCH₂), 39.6 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.9 (CH₃), 16.7 (CH₃); IR (film from CH₂Cl₂): ν_max 3188, 3080, 2967, 2905, 2791, 1678, 1568 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₅H₂₁N₄O 273.1710; Found 273.1715 (Δ = 1.8 ppm); HRESIMS/MS (40 eV) m/z (%): 137.0454 (100), 110.0349 (39).

3.2.24. 1,5-Bis((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one (47), 2,7-bis((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-2,7-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one (48), and 5-((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-5H-pyrazolo[3,4-d]pyrimidin-4-ol (49)

Allopurinol (0.48 mmol, 65.2 mg), K₂CO₃ (0.51 mmol, 70.5 mg) and farnesyl bromide (0.55 mmol, 157.0 mg) in DMF (2 mL) at 70 °C for 27 h yielded 47, 48, and 49.

Compound 47: 17.2 mg (12%), colourless oil; R_f = 0.24 (1:5 EA/PE); 2:1 E/Z, NMR data for major isomer: ¹H NMR (500 MHz, CDCl₃): δ 8.09–8.08 (m, 1H, H-6), 7.95–7.94 (m, 1H, H-3), 5.48–5.41 (m, 1H, CH=), 5.34–5.28 (m, 1H, CH=), 5.13–5.04 (m, 4H, 4 × CH=), 4.94 (d, J = 6.9 Hz, 2H, NCH₂), 4.61 (d, J = 7.2 Hz, 2H, NCH₂), 2.18–1.99 (m, 14H, 7 × CH₂), 1.99–1.92 (m, 2H, CH₂), 1.85 (s, 3H, CH₃), 1.83 (s, 3H, CH₃), 1.68 (s, 9H, 3 × CH₃), 1.60 (s, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 157.4 (C, C-4), 151.0 (C, C-7a), 148.4 (CH, C-6), 142.7 (C=), 141.0 (C=), 135.9 (C=), 135.6 (C=), 135.1 (CH, C-3), 131.49 (C=), 131.46 (C=), 124.42 (CH=), 124.37 (CH=), 123.7 (CH=), 123.5 (CH=), 118.4 (CH=), 118.3 (CH=), 105.9 (C, C-3a), 45.4 (NCH₂), 43.2 (NCH₂), 39.80 (CH₂), 39.78 (CH₂), 39.64 (CH₂), 39.59 (CH₂), 26.83 (CH₂), 26.81 (CH₂), 26.34 (CH₂), 26.30 (CH₂), 25.9 (CH₃), 25.8 (CH₃), 17.8 (2 × CH₃), 16.74 (CH₃), 16.69 (CH₃), 16.19 (CH₃), 16.15 (CH₃); IR (film from CH₂Cl₂): ν_max 3368, 2964, 2925, 2856, 1696, 1582 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₃₅H₅₃N₄O 545.4214; Found 545.4223 (Δ = 1.7 ppm); HRESIMS/MS (40 eV) m/z (%): 137.0458 (100), 81.702 (12).

Compound 48: 6.4 mg (4%), colourless oil; R_f = 0.48 (1:1 EA/PE); 3:2 E/Z, NMR data for major isomer: ¹H NMR (500 MHz, CDCl₃): δ 8.06 (s, 1H, H-3), 7.95 (s, 1H, H-6), 5.51 (t, J = 6.5 Hz, 1H, CH=), 5.32–5.26 (m, 1H, CH=), 5.13–5.05 (m, 4H, 4 × CH=), 4.89 (d, J = 7.4 Hz, 2H, NCH₂), 4.57 (d, J = 7.1 Hz, 2H, NCH₂), 2.20–1.93 (m, 16H, 8 × CH₂), 1.81 (s, 3H, CH₃), 1.79 (s, 3H, CH₃), 1.68 (s, 6H, 2 × CH₃), 1.62–1.57 (m, 12H, 4 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 158.9 (C, C-4), 158.6 (C, 7a), 148.5 (CH, C-6), 144.5 (C=), 142.1 (C=), 136.0 (C=), 135.8 (C=), 131.7 (C=), 131.4 (C=), 127.0 (CH, C-3), 124.4 (CH=), 124.3 (CH=), 123.5 (CH=), 123.4 (CH=), 118.5 (CH=), 116.7 (CH=), 107.1 (C, C-3a), 51.2 (NCH₂), 43.0 (NCH₂), 39.77 (CH₂), 39.76 (CH₂), 39.64 (CH₂), 39.62 (CH₂), 26.8 (CH₂), 26.7 (CH₂), 26.4 (CH₂), 26.3 (CH₂), 25.85 (CH₃), 25.84 (CH₃), 17.82 (CH₃), 17.81 (CH₃), 16.8 (CH₃), 16.7 (CH₃), 16.19 (CH₃), 16.15 (CH₃); IR (film from CH₂Cl₂): ν_max 3404, 2973, 2934, 1687 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₃₅H₅₃N₄O 545.4214; Found 545.4225 (Δ = 2.0 ppm); HRESIMS/MS (40 eV) m/z (%): 137.0451 (100), 81.0699 (30).

Compound 49: 33.6 mg (21%), white solid; R_f = 0.16 (1:1 EA/PE); 3:2 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 12.64 (br s, 1H, OH), 8.20 (s, 1H, H-3), 8.08 (s, 1H, H-6), 5.34–5.28 (m, 1H, CH=), 5.09–5.01 (m, 2H, 2 × CH=), 4.64 (d, J = 7.3 Hz, 2H, NCH₂), 2.14–2.04 (m, 4H, 2 × CH₂), 2.04–1.96 (m, 2H, CH₂), 1.96–1.92 (m, 2H, CH₂), 1.82 (s, 3H, CH₃), 1.64 (s, 3H, CH₃), 1.57 (s, 3H, CH₃), 1.56 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 157.4 (C, C-4), 153.3 (C, C-7a), 149.4 (CH, C-6), 143.0 (C=), 136.1 (CH, C-3) 135.9 (C=), 131.5 (C=), 124.3 (CH=), 123.4 (CH=), 118.0 (CH=), 105.9 (C, C-3a), 43.5 (NCH₂), 39.7 (CH₂), 39.6 (CH₂), 26.8 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3188, 3108, 2967, 2917, 1676 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₀H₂₉N₄O 341.2336; Found 341.2340 (Δ = 1.2 ppm); HRESIMS/MS (40 eV) m/z (%): 137.0451 (100), 110.0344 (9).

3.2.25. 1,5-Bis((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one (50)

Allopurinol (0.71 mmol, 96.6 mg), K₂CO₃ (0.81 mmol, 111.9 mg) and geranylgeranyl bromide (0.73 mmol, 259.7 mg) in DMF (1 mL) for 27 h yielded 50, 6.8 mg (2%), colourless oil. R_f = 0.26 (1:5 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 8.07 (s, 1H, H-3), 7.93 (s, 1H, H-6), 5.43 (t, J = 6.8 Hz, 1H, CH=), 5.30 (t, J = 7.2 Hz, 1H, CH=), 5.11–5.04 (m, 6H, 6 × CH=), 4.93 (d, J = 6.9 Hz, 2H, NCH₂), 4.60 (d, J = 7.3 Hz, 2H, NCH₂), 2.14–2.01 (m, 16H, 8 × CH₂), 1.99–1.93 (m, 8H, 4 × CH₂), 1.83 (s, 3H, CH₃), 1.81 (s, 3H, CH₃), 1.67 (s, 6H, 2 × CH₃), 1.59 (s, 6H, 2 × CH₃), 1.584 (s, 3H, CH₃), 1.579 (s, 6H, 2 × CH₃), 1.56 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 157.4 (C, C-4), 151.0 (C, C-7a), 148.4 (CH, C-6), 142.7 (C=), 141.1 (C=), 135.9 (C=), 135.6 (C=), 135.13 (C= and CH, C-3), 135.09 (C=), 131.43 (C=), 131.41 (C=), 124.50 (CH=), 124.48 (CH=), 124.3 (CH=), 124.2 (CH=), 123.7 (CH=), 123.5 (CH=), 118.4 (CH=), 118.3 (CH=), 105.9 (C, C-3a), 45.4 (NCH₂), 43.2 (NCH₂), 39.86 (CH₂), 39.85 (CH₂), 39.80 (CH₂), 39.78 (CH₂), 39.7 (CH₂), 39.6 (CH₂), 26.9 (2 × CH₂), 26.73 (CH₂), 26.71 (CH₂), 26.38 (CH₂), 26.35 (CH₂), 25.9 (2 × CH₃), 17.8 (2 × CH₃), 16.8 (CH₃), 16.7 (CH₃), 16.2 (2 × CH₃), 16.1 (2 × CH₃); IR (film from CH₂Cl₂): ν_max 3392, 2975, 2937, 1699 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₄₅H₆₉N₄O 681.5466; Found 681.5470 (Δ = 0.6 ppm); HRESIMS/MS (40 eV) m/z (%): 341.01647 (72), 281.0500 (20), 266.9983 (21), 221.0823 (24), 207.0312 (25), 147.0648 (32), 137.0451 (100).

3.2.26. (E)-1-(3,7-Dimethylocta-2,6-dien-1-yl)pyrimidine-2,4(1H,3H)-dione (54)

Uracil (0.53 mmol, 59.9 mg), K₂CO₃ (0.51 mmol, 68.9 mg) and geranyl bromide (0.65 mmol, 141.1 mg) in DMF (2 mL) at 50 °C for 21 h yielded 54, after modified work up and modified purification—the reaction was filtered, H₂O (6 mL) was added to the filtrate and stored in the fridge until precipitate formed. The isolated solid was recrystallised from PE, 15.8 mg (12%), white crystals. ¹H NMR (500 MHz, CDCl₃): δ 8.42 (br s, 1H, NH), 7.16 (d, J = 7.9 Hz, 1H, H-6), 5.68 (dd, J = 7.9, 2.1 Hz, 1H, H-5), 5.22 (t, J = 7.3, 1H, CH=), 5.04 (t, J = 6.6 Hz, 1H, CH=), 4.35 (d, J = 7.3 Hz, 2H, NCH₂), 2.11 (m, 4H, 2 × CH₂), 1.75 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.60 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 163.4 (C, C-4), 150.9 (C, C-2), 143.7 (C=), 143.6 (CH, C-6), 132.4 (C=), 123.5 (CH=), 117.3 (CH=), 102.2 (CH, C-5), 45.2 (NCH₂), 39.6 (CH₂), 26.2 (CH₂), 25.9 (CH₃), 17.9 (CH₃), 16.6 (CH₃); IR (neat): ν_max 3121, 2967, 2929, 2808, 1696, 1657 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₄H₂₁N₂O₂ 249.1598; Found 249.1595 (Δ = −1.2 ppm); HRESIMS/MS (40 eV) m/z (%): 113.0346 (100), 81.0700 (18), 70.0289 (7).

3.2.27. 1-((6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-yl)pyrimidine-2,4(1H,3H)-dione (55)

Uracil (0.49 mmol, 54.9 mg), K₂CO₃ (0.51 mmol, 69.9 mg) and farnesyl bromide (0.54 mmol, 150 mg) in DMF (2 mL) at 70 °C for 24 h yielded 55, after modified work up and modified purification as per 54, 38.6 mg (25%), white crystals. 3:2 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 8.46 (s, 1H, NH), 7.16 (d, J = 7.9 Hz, 1H, H-6), 5.68 (dd J = 8.0, 2.2 Hz, 1H, H-5), 5.24–5.19 (m, 1H, CH=), 5.09–5.04 (m, 2H, 2 × CH=), 4.34 (d, J = 7.3 Hz, 2H, NCH₂), 2.16–2.01 (m, 6H, 3 × CH₂), 1.99–1.94 (m, 2H, CH₂), 1.75 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.62 (s, 3H, CH₃), 1.59 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 163.5 (C, C-4), 150.9 (C, C-2), 143.8 (C=), 143.6 (CH, C-6), 136.0 (C=), 131.6 (C=), 124.3 (CH=), 123.4 (CH=), 117.2 (CH=), 102.2 (CH, C-5), 45.3 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 26.8 (CH₂), 26.2 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.6 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3054, 2979, 2930, 1683 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₉H₂₉N₂O₂ 317.2224; Found 317.2222 (Δ = −0.6 ppm); HRESIMS/MS (40 eV) m/z (%): 113.0340 (87), 81.0698 (100).

3.2.28. 1-((2Z,6E,10E)-3,7,11,15-Tetramethylhexadeca-2,6,10,14-tetraen-1-yl)pyrimidine-2,4(1H,3H)-dione (56)

Uracil (0.29 mmol, 32.7 mg), K₂CO₃ (0.36 mmol, 50.1 mg) and geranylgeranyl bromide (0.32 mmol, 111 mg) in DMF (1 mL) for 48 h yielded 56, 9.4 mg (31%), white solid. R_f = 0.23 (2:3 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 8.78 (s, 1H, NH), 7.15 (dd, J = 7.9, 0.8 Hz, 1H, H-6), 5.68 (dd, J = 7.9, 1.4 Hz, 1H, H-5), 5.22 (t, J = 7.2 Hz, 1H, CH=), 5.13–5.04 (m, 3H, 3 × CH=), 4.33 (d, J = 7.3 Hz, 2H, NCH₂), 2.20–2.10 (m, 4H, 2 × CH₂), 2.10–2.02 (m, 4H, 2 × CH₂), 2.01–1.93 (m, 4H, 2 × CH₂), 1.79 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.61 (s, 3H, CH₃), 1.60 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 163.6 (C, C-4), 150.9 (C, C-2), 143.7 (C=), 143.6 (CH, C-6), 136.5 (C=), 135.3 (C=), 131.5 (C=), 124.5 (CH=), 124.1 (CH=), 123.1 (CH=), 118.1 (CH=), 102.2 (CH, C-5), 45.1 (NCH₂), 39.9 (2 × CH₂), 32.2 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.5 (CH₂), 25.9 (CH₃), 23.6 (CH₃), 17.8 (CH₃), 16.20 (CH₃), 16.16 (CH₃); IR (film from CH₂Cl₂): ν_max 3425, 3197, 3054, 2970, 2930, 2875, 1686 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. C₂₄H₃₇N₂O₂ 385.2850; Found 385.2817 (Δ = −8.6 ppm); HRESIMS/MS (40 eV) m/z (%):113.0331 (100), 107.0389 (21).

3.2.29. (E)-1-(3,7-Dimethylocta-2,6-dien-1-yl)-5-methylpyrimidine-2,4-(1H,3H)-dione (57)

Thymine (0.48 mmol, 61.0 mg), K₂CO₃ (0.50 mmol, 69.4 mg) and geranyl bromide (0.65 mmol, 141.1 mg) in DMF (2 mL) for 20 h yielded 57, after modified work up and modified purification as per 54, 6.8 mg (5%), white crystals. ¹H NMR (600 MHz, CDCl₃): δ 8.20 (br s, 1H, NH), 6.95 (d, J = 1.2 Hz, 1H, H-6), 5.21 (t, J = 7.1 Hz, 1H, CH=), 5.05 (t, J = 6.9 Hz, 1H, CH=), 4.32 (d, J = 7.3 Hz, 2H, NCH₂), 2.14–2.05 (m, 4H, 2 × CH₂), 1.92 (s, 3H,C(5)CH₃), 1.75 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.60 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 163.9 (C, C-4), 150.8 (C, C-2), 142.9 (C=), 139.7 (CH, C-6), 132.3 (C=), 123.5 (CH=), 117.7 (CH=), 110.7 (C, C-5), 45.1 (NCH₂), 39.6 (CH₂), 26.3 (CH₂), 25.9 (CH₃), 17.9 (CH₃), 16.6 (CH₃), 12.6 (C(5)CH₃); IR (film from CH₂Cl₂): ν_max 3152, 2975, 2919, 2830, 1685, 1645 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₅H₂₃N₂O₂ 263.1754; Found 263.1752 (Δ = −0.8 ppm); HRESIMS/MS (10 eV) m/z (%): 128.0524 (7), 127.0501 (100), 81.07 (19).

3.2.30. 5-Methyl-1-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)pyrimidine-2,4(1H,3H)-dione (58)

Thymine (0.49 mmol, 62.4 mg), K₂CO₃ (0.51 mmol, 70.5 mg) and farnesyl bromide (0.62 mmol, 176.9 mg) in DMF (2 mL) at 70 °C for 24 h yielded 58, after modified work up and modified purification as per 54, 31.9 mg (20%), white crystals. 3:2 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 8.41 (br s, 1H, NH), 6.95 (s, 1H, H-6), 5.24–5.19 (m, 1H, CH=), 5.11–5.01 (m, 2H, 2 × CH=), 4.32 (d, J = 7.5 Hz, 2H, NCH₂), 2.14–2.02 (m, 6H, 3 × CH₂), 1.98–1.95 (m, 2H, CH₂), 1.91 (s, 3H, C(5)CH₃), 1.76 (s, 3H, CH₃), 1.67 (s, 6H, 2 × CH₃), 1.59 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 164.0 (C, C-4), 150.9 (C, C-2), 142.9 (C=), 139.7 (CH, C-6), 135.9 (C=), 131.6 (C=), 124.3 (CH=), 123.4 (CH=), 117.7 (CH=), 110.7 (C, C-5), 45.1 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 26.8 (CH₂), 26.2 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.2 (CH₃), 12.6 (C(5)CH₃); IR (film from CH₂Cl₂): ν_max 3177, 3052, 2966, 2927, 1665 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₀H₃₁N₂O₂ 331.2380; Found 331.2372 (Δ = −2.4 ppm); HRESIMS/MS (40 eV) m/z (%): 127.0487 (100), 110.0219 (46).

3.2.31. 5-Methyl-1-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)pyrimidine-2,4(1H,3H)-dione (59)

Thymine (0.31 mmol, 38.7 mg), K₂CO₃ (0.36 mmol, 49.6 mg) and geranylgeranyl bromide (0.32 mmol, 111 mg) in DMF (1 mL) for 24 h yielded 59, 10.1 mg (11%), waxy white solid. R_f = 0.22 (2:3 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 8.67 (br s, 1H, NH), 6.95 (d, J = 1.1 Hz, 1H, H-6), 5.21 (t, J = 6.7 Hz, 1H, CH=), 5.12–5.04 (m, 3H, 3 × CH=), 4.32 (d, J = 7.1 Hz, 2H, NCH₂), 2.16–2.02 (m, 8H, 4 × CH₂), 2.01–1.94 (m, 4H, 2 × CH₂), 1.91 (s, 3H, C(5)CH₃), 1.76 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.60 (s, 6H, 2 × CH₃), 1.59 (s, 3H, CH₃); IR (film from CH₂Cl₂): ν_max 3427, 3176, 3043, 2968, 2925, 2855, 1668 cm⁻¹; ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 164.1 (C, C-4), 151.0 (C, C-2), 143.0 (C=), 139.7 (CH, C-6), 135.9 (C=), 135.2 (C=), 131.4 (C=), 124.5 (CH=) 124.2 (CH=), 123.5 (CH=), 117.7 (CH=), 110.8 (C, C-5), 45.1 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 39.7 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.3 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.21 (CH₃), 16.15 (CH₃), 12.6 (C(5)CH₃); HRESIMS m/z: [M + H]⁺ Calcd. for C₂₅H₃₉N₂O₂ 399.3006; Found 399.2995 (Δ = −2.8 ppm); HRESIMS/MS m/z (20 eV) (%): 399.2980 (8), 283.2625 (13), 127.0501 (100).

3.2.32. N-(-1,3-Bis((E)-3,7-dimethylocta-2,6-dien-1-yl)-2-oxo-2,3-dihydropyrimidin-4(1H)-ylidene)formamide (60) and 1,3-bis((E)-3,7-dimethylocta-2,6-dien-1-yl)-4-imino-3,4-dihydropyrimidin-2(1H)-one (61)

Cytosine (1.0 mmol, 112.4 mg), K₂CO₃ (1.0 mmol, 152.0 mg) and geranyl bromide (1.1 mmol, 238.5 mg) in DMF (4 mL) for 27 h yielded 60 and 61.

Compound 60: 4.5 mg (2%), pale-yellow oil; R_f = 0.40 (1:1 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 9.14 (s, 1H, HC=O), 7.10 (d, J = 7.9 Hz, 1H, H-6), 6.4 (d, J = 7.9 Hz, 1H, H-5), 5.25 (t, J = 7.0 Hz, 1H, CH=), 5.22 (t, J = 7.0 Hz, 1H, CH=), 5.08–5.02 (m, 2H, 2 × CH=), 4.75 (d, J = 7.1 Hz, 2H, NCH₂), 4.39 (d, J = 7.2 Hz, 2H, NCH₂), 2.15–2.03 (m, 6H, 3 × CH₂), 2.02–1.97 (m, 2H, CH₂), 1.82 (s, 3H, CH₃), 1.74 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.60 (s, 3H, CH₃), 1.58 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 172.8 (HC=O), 159.8 (C, C-4), 150.5 (C, C-2), 144.3 (C=), 141.2 (C=), 140.8 (CH, C-6), 132.4 (C=), 131.7 (C=), 124.1 (CH=), 123.5 (CH=), 117.4 (CH=), 117.0 (CH=), 97.4 (CH, C-5), 46.6 (NCH₂), 42.0 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 26.6 (CH₂), 26.2 (CH₂), 25.89 (CH₃), 25.85 (CH₃), 17.89 (CH₃), 17.85 (CH₃), 16.8 (CH₃), 16.7 (CH₃); IR (film from CH₂Cl₂): ν_max 3306, 2964, 2915, 2854, 1654, 1684, 1403 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₅H₃₈N₃O₂ 412.2959; Found 412.2985 (Δ = 6.3 ppm); HRESIMS/MS m/z (10 eV) (%): 412.0949 (11), 276.1708 (35), 140.0447 (100).

Compound 61: 18.3 mg (9%), pale-yellow oil; R_f = 0.19 (1:2 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 6.59 (d, J = 7.9 Hz, 1H, H-6), 5.53 (d, J = 7.9 Hz, 1H, H-5), 5.23 (t, J = 6.3 Hz, 1H, CH=), 5.21–5.16 (m, 1H, CH=), 5.09–5.02 (m, 2H, 2 × CH=), 4.62 (d, J = 6.4 Hz, 2H, NCH₂), 4.25 (d, J = 7.2 Hz, 2H, NCH₂), 2.14–2.03 (m, 6H, 3 × CH₂), 2.01–1.97 (m, 2H, CH₂), 1.80 (s, 3H, CH₃), 1.71 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.65 (s, 3H, CH₃), 1.58 (s, 3H, CH₃), 1.57 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 158.2 (C, C-4), 151.3 (C, C-2), 142.3 (C=), 139.6 (C=), 135.2 (CH, C-6), 132.1 (C=), 131.6 (C=), 124.2 (CH=), 123.7 (CH=), 118.6 (CH=), 118.3 (CH=), 102.0 (CH, C-5), 45.7 (NCH₂), 40.5 (NCH₂), 39.8 (CH₂), 39.6 (CH₂), 26.6 (CH₂), 26.3 (CH₂), 25.83 (CH₃), 25.81 (CH₃), 17.9 (CH₃), 17.8 (CH₃), 16.7 (CH₃), 16.5 (CH₃); IR (film from CH₂Cl₂): ν_max 3305, 3083, 2966, 2915, 2855, 1651 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₄H₃₈N₃O 384.3009; Found 384.3023 (Δ = 3.6 ppm); HRESIMS/MS m/z (20 eV) (%): 384.3018 (0.1), 248.1764 (100), 113.0529 (0.09).

3.2.33. N-(-2-Oxo-1,3-bis((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-2,3-dihydropyrimidin-4(1H)-ylidene)formamide (62) and 4-imino-1,3-bis((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)-3,4-dihydropyrimidin-2(1H)-one (63)

Cytosine (1.0 mmol, 114.8 mg), K₂CO₃ (1.5 mmol, 204.4 mg) and farnesyl bromide (1.2 mmol, 341 mg) in DMF (2 mL) for 25 h yielded 62 and 63.

Compound 62: 11.3 mg (3%), pale-yellow oil; R_f = 0.16 (1:4 EA/PE); 3:2 E/Z, data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 9.13 (s, 1H, HC=O), 7.1 (d, J = 7.8 Hz, 1H, H-6), 6.39 (d, J = 7.8 Hz, 1H, H-5), 5.28–5.20 (m, 2H, 2 × CH=), 5.12–5.04 (m, 4H, 4 × CH=), 4.75 (d, J = 6.8 Hz, 2H, NCH₂), 4.39 (d, J = 7.4 Hz, 2H, NCH₂), 2.15–1.92 (m, 16H, 8 × CH₂), 1.83 (s, 3H, CH₃), 1.75 (s, 3H, CH₃), 1.67 (s, 6H, 2 × CH₃), 1.60 (s, 3H, CH₃), 1.59 (s, 6H, 2 × CH₃), 1.57 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 172.8 (C, HC=O), 159.8 (C, C-4), 150.5 (C, C-2), 144.3 (C=), 141.3 (C=), 140.8 (CH, C-6), 136.0 (C=), 135.4 (C=), 131.6 (C=), 131.4 (C=), 124.5 (CH=), 124.3 (CH=), 124.0 (CH=), 123.3 (CH=), 117.4 (CH=), 116.9 (CH=), 97.4 (CH, C-5), 46.6 (NCH₂), 42.0 (NCH₂), 39.84 (CH₂), 39.82 (2 × CH₂), 39.7 (CH₂), 26.9 (CH₂), 26.8 (CH₂), 26.5 (CH₂), 26.2 (CH₂), 25.9 (2 × CH₃), 17.852 (CH₃), 17.846 (CH₃), 16.8 (CH₃), 16.7 (CH₃), 16.22 (CH₃), 16.16 (CH₃); IR (film from CH₂Cl₂): ν_max 2964, 2915, 2854, 1650, 1450 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₃₅H₅₄N₃O₂ 548.4211; Found 548.4233 (Δ = 4.0 ppm).

Compound 63: 96.4 mg (31%), pale-yellow oil; R_f = 0.19 (1:1 EA/PE); 3:2 E/Z, data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 6.59 (d, J = 7.9 Hz, 1H, H-6), 5.53 (d, J = 7.7 Hz, 1H, H-5), 5.24 (t, J = 5.7 Hz, 1H, CH=), 5.20 (t, J = 6.8 Hz, 1H, CH=), 5.13–5.04 (m, 4H, 4 × CH=), 4.63 (d, J = 6.3 Hz, 2H, NCH₂), 4.26 (d, J = 7.2 Hz, 2H, NCH₂), 2.15–1.91 (m, 16H, 8 × CH₂), 1.81 (s, 3H, CH₃), 1.72 (s, 3H, CH₃), 1.68 (s, 9H, 3 × CH₃), 1.59 (s, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 158.2 (C, C-4), 151.4 (C, C-2), 142.3 (C=), 139.6 (C=), 135.7 (C=), 135.1 (C=), 134.9 (CH, C-6), 131.6 (C=), 131.4 (C=), 124.5 (CH=), 124.3 (CH=), 124.1 (CH=), 123.6 (CH=), 118.7 (CH=), 118.4 (CH=), 102.2 (CH, C-5), 45.7 (NCH₂), 40.4 (NCH₂), 39.84 (CH₂), 39.83 (CH₂), 39.77 (CH₂), 39.65 (CH₂), 26.9 (CH₂), 26.8 (CH₂), 26.6 (CH₂), 26.3 (CH₂), 25.0 (2 × CH₃), 17.9 (2 × CH₃), 16.7 (CH₃), 16.6 (CH₃), 16.2 (CH₃), 16.1 (CH₃); IR (film from CH₂Cl₂): ν_max 3306, 2964, 2915, 2854 1654 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₃₄H₅₄N₃O 520.4261; Found 520.4269 (Δ = 1.5 ppm); HRESIMS/MS m/z (20 eV) (%): 316.2386 (21), 112.0511 (100).

3.2.34. N-(-2-Oxo-1,3-bis((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-2,3-dihydropyrimidin-4(1H)-ylidene)formamide (64)

Cytosine (0.41 mmol, 45.7mg), K₂CO₃ (0.84 mmol, 116.4 mg), and geranylgeranyl bromide (0.42 mmol, 148.4 mg) in DMF (2 mL) for 25 h yielded 64, 6.3 mg (3%), pale-yellow oil. R_f = 0.50 (1:1 EA/PE); ¹H NMR (600 MHz, CDCl₃): δ 9.13 (s, 1H, HC=O), 7.09 (d, J = 7.9 Hz, 1H, H-6), 6.39 (d, J = 7.9 Hz, 1H, H-5), 5.26 (t, J = 7.0 Hz, 1H, CH=), 5.24 (t, J = 7.1 Hz, 1H, CH=), 5.12–5.04 (m, 6H, 6 × CH=), 4.75 (d, J = 6.8 Hz, 2H, NCH₂), 4.39 (d, J = 7.3 Hz, 2H, NCH₂), 2.17–1.94 (m, 24H, 12 × CH₂), 1.83 (s, 3H, CH₃), 1.75 (s, 3H, CH₃), 1.67 (s, 6H, 2 × CH₃), 1.60 (s, 9H, 3 × CH₃), 1.59 (s, 6H, 2 × CH₃), 1.58 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 172.8 (HC=O), 159.8 (C, C-4), 150.5 (C, C-2), 144.3 (C=), 141.3 (C=), 140.8 (C, C-6), 136.1 (C=), 135.4 (C=), 135.3 (C=), 135.1 (C=), 131.44 (C=), 131.40 (C=), 124.53 (CH=), 124.48 (CH=), 124.4 (CH=), 124.1 (CH=), 124.0 (CH=), 123.4 (CH=), 117.4 (CH=), 116.9 (CH=), 97.4 (C, C-5), 46.6 (NCH₂), 42.0 (NCH₂), 39.87 (2 × CH₂), 39.86 (CH₂), 39.84 (CH₂), 39.82 (CH₂), 39.7 (CH₂), 26.91 (CH₂), 26.90 (CH₂), 26.8 (CH₂), 26.7 (CH₂), 26.6 (CH₂), 26.3 (CH₂), 25.9 (2 × CH₃), 17.8 (2 × CH₃), 16.8 (CH₃), 16.7 (CH₃), 16.24 (CH₃), 16.18 (CH₃), 16.17 (CH₃), 16.16 (CH₃); IR (film from CH₂Cl₂): ν_max 2964, 2916, 2852, 1622, 1537, 1452 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₄₅H₇₀N₃O₂ 684.5463; Found 684.5487 (Δ = 3.5 ppm); HRESIMS/MS (20 eV) m/z (%): 684.2009 (2), 412.2985 (13), 140.0471 (100).

3.2.35. (E)-2-(3,7-Dimethylocta-2,6-dien-1-yl)isoindoline-1,3-dione (67)

Potassium phthalimide (2.6 mmol, 472.8 mg), K₂CO₃/Na₂CO₃ (1:1, 240 mg), and geranyl bromide (2.5 mmol, 543 mg) in DMF (10 mL) for 22 h yielded 67, additionally recrystallised from PE after chromatography, 170.2 mg (24%), white crystals. R_f = 0.59 (1:4 EA/PE); ¹H and ¹³C NMR, and IR data previously reported [56]; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₈H₂₂NO₂ 284.1645; Found 284.1640 (Δ = −1.8 ppm); HRESIMS/MS (40 eV) m/z (%): 160.0385 (100), 133.0287 (47), 81.0697 (22).

3.2.36. 2-((6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-yl)isoindoline-1,3-dione (68)

Potassium phthalimide (2.9 mmol, 541.2 mg), K₂CO₃/Na₂CO₃ (1:1, 1.9 g) and farnesyl bromide (2.0 mmol, 570.5 mg) in DMF (10 mL) for 19 h yielded 68, 12.8 mg (2%), colourless oil. R_f = 0.23 (1:10 EA/PE); 5:4 E/Z, ¹H and ¹³C NMR data for the (2E)-isomer previously reported [57]; NMR data for (2Z)-isomer: ¹H NMR (500 MHz, CDCl₃): δ 7.84–7.81 (m, 2H, H-5), 7.71–7.68 (m, 2H, H-6), 5.30–5.24 (m, 1H, CH=), 5.11–5.01 (m, 2H, 2 × CH=), 4.27 (d, J = 7.1 Hz, 2H, NCH₂), 2.30–2.24 (m, 2H, CH₂), 2.01–2.03 (m, 2H, CH₂), 2.03–1.97 (m, 2H, CH₂), 1.94–1.87 (m, 2H, CH₂), 1.82 (s, 3H, CH₃), 1.63 (s, 3H, CH₃), 1.56 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 168.3 (C, C-1), 140.8 (C=), 135.5 (C=), 133.9 (CH, C-6), 132.5 (C, C-4), 131.7 (C=), 124.6 (CH=), 124.4 (CH=), 123.3 (CH, C-5), 118.1 (CH=), 39.9 (CH₂), 32.1 (CH₂), 35.9 (NCH₂), 26.7 (CH₂), 26.2 (CH₂), 25.9 (CH₃), 23.5 (CH₃), 17.8 (CH₃), 16.1 (CH₃); IR (film from CH₂Cl₂): ν_max 2964, 2917, 2854, 1710 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₃H₃₀NO₂ 352.2271; Found 352.2257 (Δ = −4.0 ppm); HRESIMS/MS (40 eV) m/z (%): 250.9685 (16), 160.0391(100).

3.2.37. 2-((2E,6E,10E)-3,7,11,15-Tetramethylhexadeca-2,6,10,14-tetraen-1-yl)isoindoline-1,3-dione (69)

Following the general alkylation procedure, also previously published [58], potassium phthalimide (0.30 mmol, 54.8 mg), K₂CO₃ (1.1 mmol, 155.6 mg) and geranylgeranyl bromide (0.32 mmol, 111 mg) yielded 69, 42.6 mg (45%), colourless oil. R_f = 0.31 (1:9 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 7.83 (dd, J = 5.3, 3.0 Hz, 1H, H-5), 7.69 (dd, J = 5.4, 3.0 Hz, 1H, H-6), 5.27 (t, J = 7.2 Hz, 1H, CH=), 5.13–5.02 (m, 3H, 3 × CH=), 4.28 (d, J = 7.1 Hz, 2H, NCH₂), 2.11–1.89 (m, 12H, 6 × CH₂), 1.83 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.59 (s, 3H, CH₃), 1.57 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 168.2 (C, C-1), 140.8 (C=), 135.4 (C=), 134.9 (C=), 133.9 (CH, C-6), 132.4 (C, C-4), 131.3 (C=), 124.5 (CH=), 124.3 (CH=), 123.7 (CH=), 123.2 (CH, C-5), 118.0 (CH=), 39.8 (CH₂), 39.7 (CH₂), 39.6 (CH₂), 35.9 (NCH₂), 26.8 (CH₂), 26.7 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.5 (CH₃), 16.10 (CH₃), 16.07 (CH₃); IR (film from CH₂Cl₂): ν_max 3057, 2930, 1710 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₈H₃₈NO₂ 420.2897; Found 420.2889 (Δ = −1.9 ppm).

3.2.38. (E)-1-(3,7-Dimethylocta-2,6-dien-1-yl)-1H-imidazole (70)

Following the general alkylation procedure, also previously published [59], imidazole (0.57 mmol, 38.7 mg), K₂CO₃ (0.66 mmol, 91.4 mg) and geranyl bromide (0.60 mmol, 130 mg) in DMF (2 mL) for 46 h yielded 70, 36.7 mg (32%), colourless oil. R_f = 0.20 (1:1 EA/PE); ¹H NMR data reported previously [60]; ¹³C{¹H} NMR (120 MHz, CDCl₃): δ 141.7 (C=), 136.8 (CH, C-2), 132.2 (C=), 129.3 (CH, C-4), 123.6 (CH=), 118.7 (CH, C-5), 118.6 (CH=), 44.6 (NCH₂), 39.4 (CH₂), 26.2 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.3 (CH₃); IR (film from CH₂Cl₂): ν_max 3110, 2966, 2916, 2855 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₃H₂₁N₂ 205.1699; Found 205.1700 (Δ = 0.5 ppm); HRESIMS/MS (40 eV) m/z (%): 81.0716 (21), 79.0559 (18), 69.0470 (100).

3.2.39. 1-((6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-yl)-1H-imidazole (71)

Following the general alkylation procedure, also previously published [59], imidazole (0.51 mmol, 34.6 mg), K₂CO₃ (0.65 mmol, 90.4 mg) and farnesyl bromide (0.60 mmol, 171 mg) in DMF (2 mL) for 48 h yielded 71, 11.4 mg (8%), colourless oil. R_f = 0.10 (1:1 EA/PE); 7:2 E/Z, NMR data for major isomer: ¹H NMR (600 MHz, CDCl₃): δ 7.46 (s, 1H, H-2), 7.04 (s, 1H, H-4), 6.88 (s, 1H, H-5), 5.38–5.33 (m, 1H, CH=), 5.12–5.05 (m, 2H, 2 × CH=), 4.52 (d, J = 7.1 Hz, 2H, NCH₂), 2.19–1.94 (m, 8H, 4 × CH₂), 1.75 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.60 (s, 3H, CH₃), 1.59 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 141.9 (C=) 136.8 (CH, C-2), 135.9 (C=), 131.5 (C=), 129.2 (CH, C-4), 124.4 (CH=), 123.5 (CH=), 118.8 (CH, C-5), 118.6 (CH=), 44.7 (NCH₂), 39.8 (CH₂), 39.5 (CH₂), 26.8 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.5 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 2965, 2917, 2856 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₈H₂₉N₂ 273.2325; Found 273.2326 (Δ = 0.4 ppm); HRESIMS/MS (20 eV) m/z (%): 81.0700 (45), 69.0457 (100).

3.2.40. 1-((2E,6E,10E)-3,7,11,15-Tetramethylhexadeca-2,6,10,14-tetraen-1-yl)-1H-imidazole (72)

Imidazole (0.30 mmol, 20.4 mg), K₂CO₃ (0.34 mmol, 46.4 mg) and geranylgeranyl bromide (0.32 mmol, 111 mg) in DMF (1 mL) for 48 h yielded 72, 16.5 mg (20%), colourless oil. R_f = 0.18 (2:1 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 7.47 (s, 1H, H-2), 7.05 (s, 1H, H-4), 6.89 (s, 1H, H-5), 5.36 (t, J = 6.7 Hz, 1H, CH=), 5.12–5.05 (m, 3H, 3 × CH=), 4.52 (d, J = 7.1 Hz, 2H, NCH₂), 2.17–2.01 (m, 8H, 4 × CH₂), 2.01–1.92 (m, 4H, 2 × CH₂), 1.75 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.59 (s, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 142.0 (C=), 136.8 (CH, C-2), 135.9 (C=), 135.1 (C=), 131.4 (C=), 129.1 (CH, C-4), 124.5 (CH=), 124.2 (CH=), 123.5 (CH=), 118.8 (CH, C-5), 118.5 (CH=), 44.7 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 39.5 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.5 (CH₃), 16.2 (CH₃), 16.1 (CH₃); IR (film from CH₂Cl₂): ν_max 3118, 2966, 2925 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₃H₃₇N₂ 341.2951; Found 341.2934 (Δ = −5.0 ppm); HRESIMS/MS (40 eV) m/z (%): 121.1001 (95), 109.1006 (58), 107.0849 (100).

3.3. General Formylation Procedure for Synthesis of 73–77

3.3.1. (E)-N-(3,7-Dimethylocta-2,6-dien-1-yl)formamide (73)

Acetic anhydride (3.6 mmol, 0.336 g, 339 μL) was stirred on an ice bath for 10 min under nitrogen before addition of formic acid (3.9 mmol, 180 mg, 147.6 μL). The ice bath was removed, and the reaction was heated at 55 °C in an oil bath for 2.5 h. The reaction was cooled to RT and geranylamine (1.6 mmol, 250 mg) was added and reaction was stirred for 3 h. H₂O (3 mL) was added and the mixture was extracted with EA (3 × 2 mL). The combined extracts were washed with H₂O (3 × 2 mL), then brine (1 × 2 mL) and dried over anhydrous MgSO₄. The solvent was removed under reduced pressure and the residue was purified by silica gel flash chromatography (2:3 EA/PE, R_f = 0.21) to yield 73, 43.3 mg (30%), pale-yellow oil. ¹H and ¹³C NMR data previously reported [61]; IR (film from CH₂Cl₂): ν_max 3277, 3045, 2968, 2915, 2856, 1655 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₁H₂₀NO 182.1539; Found 182.1528 (Δ = −6.0 ppm); HRESIMS/MS (20 eV) m/z (%): 182.1502 (7), 137.1316 (100), 109.0999 (15).

3.3.2. N-((2Z,6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-yl)formamide (74) and N-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)formamide (75)

Acetic anhydride (2.5 mmol, 254 mg, 235 μL), formic acid (2.7 mmol, 124 mg, 102 μL), and farnesylamine (1.13 mmol, 250 mg) for 5 h yielded 74 and 75.

Compound 74: 3.6 mg (3%), colourless oil; R_f = 0.18 (1:2 EA/PE); 3:1 rotamers, IR, ¹H and ¹³C NMR data for the (2Z)-isomer previously reported [62]; IR (film from CH₂Cl₂): ν_max 3024, 2964, 2919, 2855, 1656 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₆H₂₈NO 250.2165; Found 250.2161 (Δ = −1.6 ppm); HRESIMS/MS (40 eV) m/z (%): 109.1014 (74), 107.0841 (100).

Compound 75: 12.2 mg (7%), colourless oil; R_f = 0.14 (1:2 EA/PE); 3:2 E/Z, 7:2 rotamers. ¹H and ¹³C NMR data for the (2E)-isomer [63] and (2Z)-isomer [62] previously reported; IR (film from CH₂Cl₂): ν_max 3279, 2965, 2916, 2854, 1657 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₆H₂₈NO 250.2165; Found 250.2159 (Δ = −2.4 ppm); HRESIMS/MS (40 eV) m/z (%): 121.0998 (60), 109.1014 (100).

3.3.3. N-((2Z,6E,10E)-3,7,11,15-Tetramethylhexadeca-2,6,10,14-tetraen-1-yl)formamide (76) and N-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)formamide (77)

Acetic anhydride (2.3 mmol, 233 mg, 215.5 μL), formic acid (2.5 mmol, 115 mg, 94 μL), and geranylgeranylamine (0.86 mmol, 250 mg) for 5 h yielded 76 and 77.

Compound 76: 3.8 mg (6%), pale-yellow oil; R_f = 0.26 (2:3 EA/PE); 2:1 rotamers, data for major rotamer: ¹H NMR (300 MHz, CDCl₃): δ 8.14 (s, 1H, HC=O), 5.28 (br s, 1H, NH), 5.21 (t, J = 8.4 Hz, 1H, CH=), 5.10 (br s, 3H, 3 × CH=), 3.88 (t, J = 6.1 Hz, 2H, NCH₂), 2.15–1.90 (m, 12H, 6 × CH₂), 1.74 (s, 3H, CH₃), 1.68 (s, 6H, 2 × CH₃), 1.60 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 160.9 (HC=O), 141.0 (C=), 136.1 (C=), 135.3 (C=), 131.5 (C=), 124.5 (CH=), 124.2 (CH=), 123.6 (CH=), 120.2 (CH=), 39.9 (2 × CH₂), 36.0 (NCH₂), 32.1 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.5 (CH₂), 25.9 (CH₃), 23.5 (CH₃), 17.8 (CH₃), 16.17 (CH₃), 16.15 (CH₃); IR (film from CH₂Cl₂): ν_max 3299, 3024, 2964, 2920, 2874, 1656 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₁H₃₆NO 318.2791; Found 318.2780 (Δ = −3.5 ppm); HRESIMS/MS (20 eV) m/z (%): 177.1655 (22), 107.0838 (100).

Compound 77: 6.5 mg (3%), pale-yellow oil; R_f = 0.21 (2:3 EA/PE); 3:1 rotamers, NMR data for major rotamer: ¹H NMR (300 MHz, CDCl₃): δ 8.16 (s, 1H, HC=O), 5.41 (br s, 1H, NH), 5.20 (t, J = 6.4 Hz, 1H, CH=), 5.10 (br s, 3H, 3 × CH=), 3.90 (t, J = 6.1 Hz, 3H, NCH₂), 2.16–1.90 (m, 12H, 6 × CH₂), 1.68 (s, 6H, 2 × CH₃), 1.60 (s, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 161.0 (HC=O), 140.8 (C=), 135.7 (C=), 135.2 (C=), 131.5 (C=), 124.5 (CH=), 124.3 (CH=), 123.8 (CH=), 119.4 (CH=), 39.9 (CH₂), 39.8 (CH₂), 39.6 (CH₂), 36.2 (NCH₂), 26.9 (CH₂), 26.8 (CH₂), 26.4 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.5 (CH₃), 16.18 (CH₃), 16.16 (CH₃); IR (film from CH₂Cl₂): ν_max 3293, 2966, 2915, 2853, 1660 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₁H₃₆NO 318.2791; Found 318.2784 (Δ = −2.2 ppm); HRESIMS/MS (20 eV) m/z (%): 121.0997 (59), 109.0998 (82), 107.0846 (100).

3.4. General Acetylation Procedure for Synthesis of 81–85

3.4.1. (E)-N-(3,7-Dimethylocta-2,6-dien-1-yl)acetamide (81)

Triethylamine (4.9 mmol, 497 mg, 681.5 μL) and geranylamine (1.6 mmol, 250.1 mg) in THF (15 mL) were stirred on an ice bath for 10 min. Acetyl chloride (2.5 mmol, 0.192 g, 174 μL) was added and the reaction was stirred for 5 h while allowed to warm to room temperature. The reaction was quenched with ice-cold H₂O (10 mL) and the mixture was extracted with EA (2 × 10 mL). The combined extracts were washed with H₂O (2 × 10 mL), then brine (1 × 10 mL) and dried over anhydrous MgSO₄. The solvent was removed under reduced pressure and the resulting residue was purified by silica gel flash chromatography (1:1 EA/PE, R_f = 0.16) to yield 81, 96.1 mg (30%), pale-yellow oil. ¹H and ¹³C NMR data previously reported [39]; IR (film from CH₂Cl₂): ν_max 3293, 3081, 2970, 2928, 1649 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₂H₂₂NO 196.1696; Found 196.1708 (Δ = 6.1 ppm); HRESIMS/MS (40 eV) m/z (%): 137.1312 (34), 109.0997 (79), 107.0473 (100).

3.4.2. N-((2Z,6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-yl)acetamide (82) and N-((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)acetamide (83)

Triethylamine (3.4 mmol, 342.7 mg, 472 μL), farnesylamine (1.1 mmol, 250.1 mg), and acetyl chloride (1.7 mmol, 133 mg, 120 μL) yielded 82 and 83.

Compound 82: 9.9 mg (9%), pale-yellow oil; R_f = 0.30 (1:1 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 5.32 (br s, 1H, NH), 5.20 (t, J = 7.2 Hz, 1H, CH=), 5.12–5.04 (m, 2H, 2 × CH=), 3.82 (t, J = 6.5 Hz, 2H, NCH₂), 2.11–1.97 (m, 8H, 4 × CH₂), 1.96 (s, 3H, CH₃C=O), 1.72 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.60 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 169.9 (C=O), 140.5 (C=), 135.9 (C=), 131.6 (C=), 124.3 (CH=), 123.6 (CH=), 120.7 (CH=), 39.9 (CH₂), 37.5 (NCH₂), 32.03 (CH₂), 26.8 (CH₂), 26.5 (CH₂), 25.9 (CH₃), 23.5 (CH₃C=O), 23.4 (CH₃), 17.9 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3293, 3086, 2967, 2927, 2857, 1653 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₇H₃₀NO 264.2322; Found 264.2319 (Δ = −1.1 ppm); HRESIMS/MS (40 eV) m/z (%): 109.1008 (95), 107.0850 (100).

Compound 83: 65.1 mg (37%), pale-yellow oil; R_f = 0.22 (1:1 EA/PE); ¹H NMR data previously reported [41]; ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 169.9 (C=O), 140.3 (C=), 135.6 (C=), 131.5 (C=), 124.4 (CH=), 123.8 (CH=), 119.9 (CH=), 39.8 (CH₂), 39.6 (CH₂), 37.8 (NCH₂), 26.9 (CH₂), 26.4 (CH₂), 25.9 (CH₃), 23.4 (CH₃C=O), 17.8 (CH₃), 16.4 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3279, 3080, 2966, 2917, 2855, 1659 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₇H₃₀NO 264.2322; Found 264.2318 (Δ = −1.5 ppm); HRESIMS/MS (40 eV) m/z (%): 109.1000 (57), 107.0843 (100).

3.4.3. N-((2Z,6E,10E)-3,7,11,15-Tetramethylhexadeca-2,6,10,14-tetraen-1-yl)acetamide (84) and N-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)acetamide (85)

Triethylamine (3.16 mmol, 319 mg, 440 μL), geranylgeranylamine (1.0 mmol, 300 mg), and acetyl chloride (1.1 mmol, 89 mg, 81 μL) in THF (10 mL) yielded 84 and 85.

Compound 84: 22.1 mg (27%), pale-yellow oil; R_f = 0.20 (1:1 EA/PE); ¹H NMR (300 MHz, CDCl₃): δ 5.38 (br s, 1H, NH), 5.19 (t, J = 7.2 Hz, 1H, CH=), 5.14–5.03 (m, 3H, 3 × CH=), 3.81 (t, J = 6.1 Hz, 2H, NCH₂), 2.13–1.96 (m, 12H, 6 × CH₂), 1.95 (s, 3H, CH₃C=O), 1.72 (s, 3H, CH₃), 1.67 (s, 3H, CH₃), 1.59 (s, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 169.9 (C=O), 140.4 (C=), 136.0 (C=), 135.2 (C=), 131.4 (C=), 124.5 (CH=), 124.2 (CH=), 123.6 (CH=), 120.7 (CH=), 39.9 (CH₂), 39.8 (CH₂), 37.5 (NCH₂), 32.1 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.5 (CH₂), 25.8 (CH₃), 23.5 (CH₃), 23.4 (CH₃C=O), 17.8 (CH₃), 16.2 (CH₃), 16.1 (CH₃); IR (film from CH₂Cl₂): ν_max 3277, 3078, 2965, 2916, 2855, 1649 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₂H₃₈NO 332.2948; Found 332.2951 (Δ = 0.9 ppm); HRESIMS/MS (10 eV) m/z (%): 332.2946 (100), 273.2567 (9).

Compound 85: 95.5 mg (38%), pale-yellow oil; R_f = 0.14 (1:1 EA/PE); ¹H NMR (300 MHz, CDCl₃): δ 5.32 (br s, 1H, NH), 5.19 (t, J = 6.8 Hz, 1H, CH=), 5.15–5.04 (m, 3H, 3 × CH=), 3.84 (t, J = 6.1 Hz, 2H, NCH₂), 2.15–1.98 (m, 12H, 6 × CH₂), 1.67 (s, 6H, 2 × CH₃), 1.60 (s, 9H, 3 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 169.9 (C=O), 140.3 (C=), 135.6 (C=), 135.2 (C=), 131.5 (C=), 124.5 (CH=), 124.3 (CH=), 123.8 (CH=), 119.9 (CH=), 39.9 (CH₂), 39.8 (CH₂), 39.6 (CH₂), 37.8 (NCH₂), 26.9 (CH₂), 26.8 (CH₂), 26.5 (CH₂), 25.9 (CH₃), 23.5 (CH₃C=O), 17.8 (CH₃), 16.5 (CH₃), 16.19 (CH₃), 16.17 (CH₃); IR (film from CH₂Cl₂): ν_max 3279, 3078, 2967, 2917, 2854, 1650 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₂H₃₈NO 332.2948; Found 332.2934 (Δ = −4.2 ppm); HRESIMS/MS (20 eV) m/z (%): 332.3002 (100), 149.1318 (100).

3.5. General Methylation Procedure for Synthesis of 78–80 and 86–88

3.5.1. (E)-N-(3,7-Dimethylocta-2,6-dien-1-yl)-N-methylformamide (78)

Freshly powdered KOH (0.647 mmol, 36.3 mg) was stirred in dry DMSO (0.1 mL) for 10 min before the addition of 73 (0.17 mmol, 30.0 mg, in 0.1 mL DMSO), followed immediately by the addition of methyl iodide (0.5 mmol, 71.0 mg, 31.2 µL). The reaction was stirred for 2.5 h, then poured onto H₂O (3 mL) and extracted with DCM (3 × 2 mL). The combined extracts were washed with H₂O (5 × 2 mL), then brine (1 × 2 mL) and dried over anhydrous MgSO₄. The solvent was removed under reduced pressure and the resulting residue was purified by silica gel flash chromatography (1:3 EA/PE, R_f = 0.14) to yield 78, 17.4 mg (54%), colourless oil. 3:2 rotamers, data for major rotamer: ¹H NMR (500 MHz, CDCl₃): δ 8.06 (s, 1H, HC=O), 5.11–5.06 (m, 1H, CH=), 5.06–5.01 (m, 1H, CH=), 3.80 (d, J = 7.1 Hz, 2H, NCH₂), 2.78 (s, 3H, NCH₃), 2.13–1.99 (m, 4H, 2 × CH₂), 1.67 (s, 3H, CH₃), 1.66 (s, 3H, CH₃), 1.58 (s, 3H, CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 162.5 (HC=O), 141.3 (C=), 132.1 (C=), 123.7 (CH=), 119.1 (CH=), 47.2 (NCH₂), 39.7 (CH₂), 29.2 (NCH₃), 26.3 (CH₂), 25.8 (CH₃), 17.8 (CH₃), 16.30 (CH₃); IR (film from CH₂Cl₂): ν_max 2965, 2917, 2854, 1663 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₂H₂₂NO 196.1696; Found 196.1695 (Δ = −0.5 ppm); HRESIMS/MS (20 eV) m/z (%): 196.1694 (17), 137.1319 (100), 109.1010 (21).

3.5.2. N-Methyl-N-((6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)formamide (79)

KOH (0.18 mmol, 9.9 mg), 75 (0.040 mmol, 10 mg), and methyl iodide (0.12 mmol, 17 mg, 7.5 µL) yielded 79, 5.9 mg (56%), colourless oil. R_f = 0.10 (1:4 EA/PE); 3:2 E/Z, 3:2 rotamers, data for major isomer and rotamer: ¹H NMR (500 MHz, CDCl₃): δ 8.08 (s, 1H, CH=O), 5.13–5.04 (m, 3H, 3 × CH=), 3.81 (d, J = 7.0 Hz, 2H, NCH₂), 2.79 (s, 3H, NCH₃), 2.14–2.00 (m, 6H, 3 × CH₂), 1.99–1.93 (m, 2H, CH₂), 1.68 (s, 3H, CH₃), 1.64 (s, 3H, CH₃), 1.59 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 162.6 (HC=O), 141.4 (C=), 135.8 (C=), 131.5 (C=), 124.4 (CH=), 123.6 (CH=), 119.0 (CH=), 47.3 (NCH₂), 39.9 (CH₂), 39.7 (CH₂), 29.2 (NCH₃), 26.8 (CH₂), 26.3 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.4 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 3495, 2963, 2916, 2853, 1677, 1665 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₇H₃₀NO 264.2322; Found 264.2324 (Δ = 0.8 ppm); HRESIMS/MS (40 eV) m/z (%): 109.1001 (53), 107.085 (100), 105.0694 (58).

3.5.3. N-Methyl-N-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)formamide (80)

KOH (0.201 mmol, 11.3 mg), 77 (0.047 mmol, 15 mg), and methyl iodide (0.14 mmol, 20 mg, 8.8 µL) yielded 80, 5.2 mg (33%), colourless oil. R_f = 0.13 (1:3 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 8.08 (s, 1H, HC=O), 5.15–5.03 (m, 4H, 4 × CH=), 3.81 (d, J = 7.0 Hz, 2H, NCH₂), 2.79 (s, 3H, NCH₃), 2.14–2.01 (m, 8H, 4 × CH₂), 2.01–1.88 (m, 4H, 2 × CH₂), 1.69 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.61 (s, 3H, CH₃), 1.60 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 162.5 (HC=O), 141.4 (C=), 135.8 (C=), 135.2 (C=), 131.4 (C=), 124.5 (CH=), 124.2 (CH=), 123.6 (CH=), 119.0 (CH=), 47.3 (NCH₂), 39.9 (CH₂), 39.8 (CH₂), 39.7 (CH₂), 29.2 (NCH₃), 26.9 (CH₂), 26.7 (CH₂), 26.4 (CH₂), 25.9 (CH₃), 17.8 (CH₃), 16.4 (CH₃), 16.18 (CH₃), 16.15 (CH₃); IR (film from CH₂Cl₂): ν_max 2962, 2922, 2854, 1681 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₂H₃₈NO 332.2948; Found 322.2955 (Δ = 2.2 ppm); HRESIMS/MS (40 eV) m/z (%): 123.1153 (39), 121.003 (51), 109.1002 (74), 107.0846 (100).

3.5.4. N-Methyl-N-((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)acetamide (86)

KOH (0.18 mmol, 9.9 mg), 83 (0.04 mmol, 10 mg), and methyl iodide (0.12 mmol, 17 mg, 7.5 μL) yielded 86, 5.9 mg (53%), colourless oil. R_f = 0.06 (1:4 EA/PE); E/Z 2:1, NMR data major isomer and rotamer: ¹H NMR (500 MHz, CDCl₃): δ 5.15–5.06 (m, 3H, 3 × CH=), 4.01 (d, J = 7.0 Hz, 2H, NCH₂), 2.91 (s, 3H, CH₃C=O), 2.14–2.10 (m, 2H, CH₂), 2.10 (s, 3H, NCH₃), 2.07–2.02 (m, 4H, 2 × CH₂), 2.00–1.95 (m, 2H, CH₂), 1.69 (s, 3H, CH₃), 1.68 (s, 3H, CH₃), 1.61 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 170.3 (C=O), 139.6 (C=), 135.7 (C=), 131.5 (C=), 124.4 (CH=), 123.6 (CH=), 119.6 (CH=), 48.7 (NCH₂), 39.9 (CH₂), 39.6 (CH₂), 35.2 (NCH₃), 26.9 (CH₂), 26.4 (CH₂), 25.8 (CH₃), 21.6 (CH₃C=O), 17.8 (CH₃), 16.4 (CH₃), 16.2 (CH₃); IR (film from CH₂Cl₂): ν_max 2964, 2916, 2856, 1646 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₁₈H₃₂NO 278.2478; Found 278.2482 (Δ = 1.4 ppm); HRESIMS/MS (40 eV) m/z (%): 121.0993(33), 107.0841 (100).

3.5.5. N-Methyl-N-((2Z,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)acetamide (87)

KOH (0.32 mmol, 18.0 mg), 84 (0.051 mmol, 17.0 mg), and methyl iodide (0.15 mmol, 21.8 mg, 9.6 μL) yielded 87, 5.1 mg (29%), colourless oil. R_f = 0.04 (1:5 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 5.17–5.07 (m, 4H, 4 × CH=), 4.00 (d, J = 7.0 Hz, 2H, NCH₂), 2.92 (s, 3H, NCH₃), 2.14–2.10 (m, 4H, 2 × CH₂), 2.10 (s, 3H, CH₃C=O), 2.09–2.04 (m, 4H, 2 × CH₂), 2.03–1.97 (m, 4H, 2 × CH₂), 1.77 (s, 3H, CH₃), 1.69 (s, 3H, CH₃), 1.63 (s, 3H, CH₃), 1.61 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 170.5 (C=O), 139.8 (C=), 136.1 (C=), 135.8 (C=), 131.4 (C=), 124.5 (CH=) 124.3 (CH=), 124.1 (CH=), 120.3 (CH=), 48.5 (NCH₂), 39.9 (2 × CH₂), 35.3 (NCH₃), 32.2 (CH₂), 26.9 (CH₂), 26.7 (CH₂), 26.4 (CH₂), 25.9 (CH₃), 23.5 (CH₃), 22.0 (CH₃C=O), 17.8 (CH₃), 16.17 (CH₃), 16.16 (CH₃); IR (film from CH₂Cl₂): ν_max 2965, 2915, 2854, 1650 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₃H₄₀NO 346.3104; Found 346.3113 (Δ = 2.6 ppm); HRESIMS/MS (40 eV) m/z (%):123.1163 (46), 121.1009 (100).

3.5.6. N-Methyl-N-((2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)acetamide (88)

KOH (0.36 mmol, 20.3 mg), 85 (0.090 mmol, 30 mg), and methyl iodide (0.27 mmol, 38.7 mg, 17.0 μL) yielded 88, 4.7 mg (15%), colourless oil. R_f = 0.05 (1:5 EA/PE); ¹H NMR (500 MHz, CDCl₃): δ 5.17–5.07 (m, 4H, 4 × CH=), 4.02 (d, J = 7.0 Hz, 2H, NCH₂), 2.92 (s, 3H, NCH₃), 2.15–2.11 (m, 4H, 2 × CH₂), 2.10 (s, 3H, CH₃C=O), 2.08–2.04 (m, 4H, 2 × CH₂), 2.01–1.96 (m, 4H, 2 × CH₂), 1.69 (s, 6H, 2 × CH₃), 1.64 (s, 3H, CH₃), 1.61 (s, 6H, 2 × CH₃); ¹³C{¹H} NMR (150 MHz, CDCl₃): δ 170.5 (C=O), 139.7 (C=), 135.7 (C=), 135.2 (C=), 131.4 (C=), 124.5 (CH=), 124.2 (CH=), 123.6 (CH=), 119.4 (CH=), 48.7 (NCH₂), 39.9 (2 × CH₂), 39.6 (CH₂), 35.2 (NCH₃), 26.9 (2 × CH₂), 26.4 (CH₂), 25.9 (CH₃), 22.0 (CH₃C=O), 17.8 (CH₃), 16.4 (CH₃), 16.19 (CH₃), 16.15 (CH₃); IR (film from CH₂Cl₂): ν_max 2965, 2916, 2854, 1650 cm⁻¹; HRESIMS m/z: [M + H]⁺ Calcd. for C₂₃H₄₀NO 346.3104; Found 346.3109 (Δ = 1.4 ppm); HRESIMS/MS (40 eV) m/z (%):123.1161 (47), 107.0851 (100).

3.6. Purification of TbHsp70, TbHsp70.4, HsHSPA8 and HsDNAJB2

Escherichia coli (E. coli.) bacterial cells transformed with the respective expression vector were grown at 37 °C in 2× YT medium supplemented with respective antibiotic and were grown to mid-logarithmic phase (A₆₀₀ 0.4–0.6). Protein production was induced by the addition of 1 mM IPTG (isopropyl-β-d-thiogalactopyranoside), and the bacterial cultures were incubated at 37 °C for 3 h for TbHsp70, HsHSPA8, HsDNAJB2, and 1 h for TbHsp70.4. Bacterial cells expressing the recombinant proteins were harvested by centrifugation (10,000 g; 15 min; 4 °C) and the cell pellet was resuspended in lysis buffer (100 mM Tris-HCl, pH 7.5, 300 mM NaCl, 20 mM imidazole, 1 mM PMSF, 1 mg/mL lysozyme), allowed to stand for 30 min at room temperature and then frozen at −80 °C overnight. The cells were then thawed on ice and sonicated at 4 °C. The resulting lysate was cleared by centrifugation (13,000 g, 40 min, 4 °C) and the supernatant was incubated with cOmplete His-tag purification resin (Roche, Germany) and allowed to bind overnight at 4 °C with gentle agitation. The resin was then pelleted by centrifugation (4500 g; 4 min) to remove unbound proteins and washed three times using native wash buffer (100 mM Tris-HCl, pH 7.5, 300 mM NaCl, 50 mM imidazole, 1 mM PMSF) to remove non-specific contaminants. The bound protein was eluted three times by re-suspending the resin in elution buffer (10 mM Tris-HCl, pH 7.5, 300 mM NaCl, 750 mM imidazole). The eluted proteins were extensively dialyzed using SnakeSkin dialysis tubing (Pierce-MWCO 10,000; Thermo Scientific, Waltham, MA, USA) into the appropriate assay buffer for functional studies and then subsequently concentrated against PEG 20,000 (Merck, Darmstadt, Germany). The protein yield was estimated using the Bradford assay (Sigma-Aldrich, St. Louis, MO, USA) with BSA as the standard. SDS-PAGE (10%) and Western analysis using mouse monoclonal anti-His primary antibody and HRP-conjugated goat anti-mouse IgG secondary antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) were conducted to assess the expression and purification of the recombinant proteins (Figure S5). HRP-conjugated goat anti-rabbit (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) was used as the secondary antibody. Imaging of the protein bands on the blot was conducted using the ECL kit (Thermo Scientific, Waltham, MA, USA) as per the manufacturer’s instructions. Images were captured using the ChemiDoc Imaging system (Bio-Rad, Hercules, CA, USA).

3.7. Purification of Tbj2

Recombinant N-terminal His-tagged Tbj2 was expressed and purified under native conditions using nickel affinity chromatography from E. coli BL21 (DE3) cells as previously described [64]. Samples were dialyzed into the appropriate assay buffer for functional studies.

3.8. Growth and Maintenance of T. b. brucei Cultures

Bloodstream form T. b. brucei Lister 927 variant 221 strain parasites were cultured in filter sterilized complete Iscoves Modified Dulbeccos Media (IMDM) based HM1-9 medium (IMDM base powder, 3.6 mM sodium bicarbonate, 1 mM hypoxanthine, 1 mM sodium pyruvate, 0.16 mM thymidine, 0.05 mM bathocuprone sulphate acid, 10% (v/v) heat inactivated foetal bovine serum, 1.5 mM L-cysteine, 0.2 mM β-mercaptoethanol, pH 7.5) in a humidified chamber at 37 °C with an atmosphere of 5% CO₂. Parasite growth was monitored using a Neubauer haemocytometer to count the cell number, after which cells were diluted, according to their density, in the described pre-warmed media.

3.9. Assessment of the Anti-Trypanosomal Activity of the Compounds on Bloodstream Form T. b. brucei Parasites

All compounds of interest were resuspended to stocks of 30 mM in DMSO and stored at −80 °C prior to use in in vitro experiments. For assessment of anti-trypanosomal activity, compounds were added to in vitro cultures of bloodstream form T. b. brucei parasites (1 × 10⁵ cells/mL) in 96-well plates at a fixed concentration of 20 μM. After an incubation period of 48 h, the number of parasites surviving drug exposure were determined using a resazurin-based cytotoxicity assay [65]. Resazurin is an oxidation–reduction sensitive dye that is reduced by living cells to resorufin. Resorufin is a fluorophore (Excitation₅₆₀/Emission₅₉₀) and thus was quantified in a multi-well fluorescence plate reader. Results are expressed as % parasite viability—the resorufin fluorescence in small molecule-treated wells relative to untreated controls. Assessment of each small molecule was conducted in duplicate, with error bars representing standard deviation (SD). The compounds that displayed 80% parasite growth inhibition when tested at 20 μM were evaluated in a dose–response experiment. The compounds were added to in vitro cultures of bloodstream form T. b. brucei parasites (1 × 10⁵ cells/mL) in 96-well plates in a 3-fold dilution series with 100 μM as the highest concentration. After an incubation period of 48 h, the number of parasites surviving drug exposure were determined as previously mentioned. The % parasite viability was determined as previously mentioned. The IC₅₀ (the concentration of compound required to decrease cell viability of T. b. brucei parasites by 50% compared to those grown in the absence of the compound) values for each of the compounds were determined from a dose–response curve by non-linear regression generated using GraphPad Prism^® (v. 7.0; San Diego, CA, USA) software. For comparative purposes, pentamidine at a fixed concentration of 1 μM was employed as a drug standard.

3.10. Growth and Maintenance of HeLa Cells

HeLa cells were grown and maintained in a culture medium comprised of Dulbecco’s Modified Eagle’s Medium (DMEM) with 5 mM L-glutamine (Lonza, Basel, Switzerland), supplemented with 10% (v/v) heat inactivated foetal bovine serum and antibiotics (penicillin/streptomycin/fungizone-PSF) in a humidified chamber at 37 °C with an atmosphere of 5% CO₂. To carry out passaging of cells, cultures were treated with 1% trypsin (w/v) to lift cells, which were then washed in 1 × phosphate buffered saline (PBS) before being re-seeded into culture flasks.

3.11. Assessment of the Cytotoxicity of the Small Molecules on a Mammalian Cell Line

For assessment of the cytotoxic effects, the small molecules were added to HeLa cells at a fixed concentration of 20 μM. After an incubation period of 24 h, the number of cells surviving drug exposure were determined using a resazurin-based cytotoxicity assay. The resazurin-based cytotoxicity assay was conducted as previously mentioned. Results were expressed as % cell—the resorufin fluorescence in small molecule-treated wells relative to untreated controls. Assessment of each small molecule was conducted in duplicate, with error bars representing standard deviation (SD). For comparative purposes, emetine at a fixed concentration of 10 μM was employed as a drug standard.

3.12. MDH Aggregation Suppression Assay

The described assay was adapted as a tool for screening the modulatory effects of the selected compounds on the chaperone function of the T. brucei Hsp70s. As an initial screen of the modulatory effects of the compounds on the chaperone function of the Hsp70s, compounds were used at a concentration of 300 μM. For compounds identified to have a modulatory effect on the chaperone function of Hsp70, a concentration-dependency experiment was conducted where the screening expanded the concentration range (0, 50, 150, and 300 μM) of the compound. These experiments were conducted on three independently purified batches of proteins, but due to limited availability of the selected compounds, each assay was conducted in duplicate on each batch of protein. Several controls were incorporated into the study. To evaluate the effect of DMSO on the chaperone activity of the Hsp70s, the solvent was added to a final concentration of 1% (v/v) in the assay with MDH and the T. brucei Hsp70. The selected compounds were also assayed (at the maximum concentration: 300 μM) together with MDH (no chaperone) to rule out that the compounds were causing an increase or decrease in MDH aggregation; and together with chaperones (no MDH) to ensure that the modulatory of the chaperone activity was not due to aggregation of the Hsp70 in the presence of compounds. The assays were performed in duplicate on three independently purified batches of proteins.

3.13. ATPase Activity Assay

The determination of the ATPase activity of the Hsp70 proteins was performed using the high throughput colorimetric ATPase assay kit (Innova Biosciences, Cambridge, UK). This method enables the quantification of the inorganic phosphate (Pi) released from ATP hydrolysis by an enzyme. Briefly, the molecular chaperones were prepared in ATPase assay buffer (100 mM Tris-HCl, 7.5, 2 mM MgCl₂, 50 mM KCl, 0.5 mM DTT) and incubated with ATP (0–2 mM) for 1 h at 37 °C. A negative control did not contain the enzyme. The samples containing Pi hydrolysed from ATP were incubated with the PiColorLock™ solution, which is a malachite green dye solution that in the presence of Pi changes absorbance due to generation of molybdate-phosphate complexes. The absorbance was measured at 595 nm using a Powerwave 96-well plate reader (BioTek Instruments, Inc., Winooski, VT, USA), and absorbance values were converted to phosphate concentrations using a standard curve of absorbance vs. phosphate concentration based on a set of Pi standards provided by the supplier assayed along with the samples. All samples were corrected for spontaneous breakdown of ATP observed in a control experiment in the absence of protein. The trypanosomal and human Hsp70s were used at 0.8 μM, and the J-proteins were used at 0.4 μM. An initial screen of the modulatory effects of the compounds on the ATPase activity of the Hsp70s was conducted using 300 μM of the compounds. Compounds identified to have a modulatory effect on the basal and J-protein-stimulated ATPase activity of Hsp70 were used at varying concentrations. The modulatory effect of the selected compounds on the basal and J-protein stimulated ATPase activity of the T. brucei Hsp70s was represented as fold change with the basal and J-protein stimulated ATPase activity of the Hsp70s taken as 1 respectively. These experiments were conducted on three independently purified batches of proteins, but due to limited availability of the selected compounds, each assay was conducted in duplicate on each batch of protein. In control reactions to evaluate the effect of DMSO on basal and J-protein stimulated ATPase activity of the Hsp70s, the solvent was added to a final concentration of 1% (v/v) in the assay. The assays were performed in duplicate on three independently purified batches of proteins.

4. Conclusions

In conclusion, a large number of malonganenone and nuttingin analogues have been synthesized and assessed for SAR relating to the anti-trypanosomal activity. Some relevant observations include a general increase in anti-parasitic activity with increasing lipophilicity (side-chain length) and certain head-groups are responsible for heightened activity over others. Of particular note is that the greatest anti-parasitic activity was achieved with compounds 47 and 48, which share the same head-group but vary in the position of the side chain, indicating flexibility within the SAR to allow for variation around both geometry and position of the lipophilic portion of the drug. In addition, cytotoxicity studies have shown that the compounds tested are generally inactive against mammalian cells, while biochemical studies have shown that these compounds potentially modulate the Hsp70 basal chaperone activity, but not the co-chaperone-stimulated chaperone activity. Overall, our study has therefore opened a new series of drug leads and could support the rational design of more active chemotherapeutic agents in the future. Such work is ongoing in our laboratories.