Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production
Abstract
:1. Introduction
2. Results and Discussion
2.1. Evaluation of Oleaginous Microalga L. bisecta SAG2043 for Lipid and AA Production
2.1.1. Effects of Different Nitrogen Concentrations, Light Intensity, and Medium Replacement on the Growth of L. bisecta SAG2043
2.1.2. Effects of Different Nitrogen Concentrations, Light Intensity and Medium Replacement on the Lipid Content of L. bisecta SAG2043
2.1.3. Effects of Different Nitrogen Concentrations, Light Intensity, and Medium Replacement on the Fatty Acids Profile of L. bisecta SAG2043
2.1.4. The Proportion of AA within the Lipid Components of L. bisecta SAG2043
2.1.5. Effects of Different Nitrogen Concentrations, Light Intensity and Medium Replacement on the Lipid and AA Productivity of L. bisecta SAG2043
2.2. Transcriptome Analysis of the Novel Oleaginous Microalga L. bisecta SAG2043 Revealed Its Potential Mechanism for AA Synthesis
3. Materials and Methods
3.1. Algal Strain and Culture Conditions
3.2. Experimental Design
3.2.1. Interactive Effects of Nitrogen Concentrations and Light Intensities on the Growth of L. bisecta SAG2043
3.2.2. The Effects of Different Nitrogen Concentrations and Medium Replacement Treatments on the Growth of L. bisecta SAG2043
3.3. Analysis Methods
3.3.1. Biomass Measurement
3.3.2. Lipid Extraction and Determination
3.3.3. Fatty Acids Analysis
3.3.4. Separation of Lipid Fractions and Quantification
3.3.5. RNA Extraction, Library Construction, Sequencing, Assembly and Functional Annotation
3.3.6. Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) Analysis
3.3.7. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
References
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Assembly | Results | Annotation | Results |
---|---|---|---|
Total Number | 43,440 | COG | 4690 |
Total Length (bp) | 41,805,231 | GO | 5695 |
Mean Length (bp) | 962.37 | KEGG | 4404 |
N50 Length (bp) | 2833 | KOG | 6267 |
200–300 | 17,294 (39.81%) | Pfam | 8398 |
300–500 | 12,004 (27.63%) | Swiss-Prot | 6080 |
500–1 kbp | 5146 (11.85%) | eggNOG | 9068 |
1 kbp–2 kbp | 2930 (6.74%) | Nr | 10,116 |
>2 kbp | 6066 (13.96%) | All annotated | 10,655 |
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Gao, B.; Huang, L.; Lei, X.; Meng, G.; Liu, J.; Zhang, C. Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production. Mar. Drugs 2020, 18, 229. https://doi.org/10.3390/md18050229
Gao B, Huang L, Lei X, Meng G, Liu J, Zhang C. Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production. Marine Drugs. 2020; 18(5):229. https://doi.org/10.3390/md18050229
Chicago/Turabian StyleGao, Baoyan, Luodong Huang, Xueqing Lei, Ge Meng, Jianguo Liu, and Chengwu Zhang. 2020. "Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production" Marine Drugs 18, no. 5: 229. https://doi.org/10.3390/md18050229