Anti-Aging Effects of R-Phycocyanin from Porphyra haitanensis on HUVEC Cells and Drosophila melanogaster
Abstract
:1. Introduction
2. Results
2.1. Isolation and Purification of R-PC
2.2. Antioxidant Capacity In Vitro
2.3. R-PC Inhibits H2O2-Induced Cytotoxicity in HUVEC Cells
2.4. P-RC Protects against H2O2-Induced Apoptotic Cell Death in HUVEC Cells
2.5. R-PC Prevents H2O2-Induced Cell Cycle Progression at the G2/M Phase in HUVEC Cells
2.6. Effect of R-PC on the Lifespan of Drosophila melanogaster and the Climbing Ability of Drosophila melanogaster
2.7. Effect of R-PC on ROS Levels and Antioxidant-Related Gene Expression in Drosophila melanogaster
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Isolation and Purification of R-PC
4.3. Antioxidant Capacity In Vitro
4.4. Cell Cultures
4.5. CCK-8 Assay
4.6. SA β-Gal Staining
4.7. Hoechst and PI Staining Kit
4.8. Annexin V-Fluorescein Isothiocyanate (FITC)/PI Double Staining and Flow Cytometry
4.9. Mitochondrial Membrane Potential Analysis
4.10. Cell Cycle Analysis
4.11. Measurement of Intracellular ROS Levels and Flow Cytometry
4.12. Survival and Life Span Studies
4.13. Climbing Ability Assays
4.14. Western Blot Assay
4.15. Quantitative Polymerase Chain Reaction (q-PCR) Assay
4.16. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Feng, Y.; Lu, H.; Hu, J.; Zheng, B.; Zhang, Y. Anti-Aging Effects of R-Phycocyanin from Porphyra haitanensis on HUVEC Cells and Drosophila melanogaster. Mar. Drugs 2022, 20, 468. https://doi.org/10.3390/md20080468
Feng Y, Lu H, Hu J, Zheng B, Zhang Y. Anti-Aging Effects of R-Phycocyanin from Porphyra haitanensis on HUVEC Cells and Drosophila melanogaster. Marine Drugs. 2022; 20(8):468. https://doi.org/10.3390/md20080468
Chicago/Turabian StyleFeng, Yanyu, Hanjin Lu, Jiamiao Hu, Baodong Zheng, and Yi Zhang. 2022. "Anti-Aging Effects of R-Phycocyanin from Porphyra haitanensis on HUVEC Cells and Drosophila melanogaster" Marine Drugs 20, no. 8: 468. https://doi.org/10.3390/md20080468