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Marine Drugs
Volume 20
Issue 8
10.3390/md20080516
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Article

In Vitro Antioxidant and Antiaging Activities of Collagen and Its Hydrolysate from Mackerel Scad Skin (Decapterus macarellus)

by
Elisa Herawati
1,*,
Yochidamai Akhsanitaqwim
1,
Pipin Agnesia
1,
Shanti Listyawati
1,
Artini Pangastuti
1 and
Adi Ratriyanto
2
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Surakarta 57126, Indonesia
2
Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta 57126, Indonesia
*
Author to whom correspondence should be addressed.
Mar. Drugs 2022, 20(8), 516; https://doi.org/10.3390/md20080516
Submission received: 28 July 2022 / Revised: 7 August 2022 / Accepted: 11 August 2022 / Published: 13 August 2022
(This article belongs to the Section Biomaterials of Marine Origin)
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Abstract

The skin of mackerel scad fish (Decapterus macarellus) is a new source for pepsin-soluble collagen and its hydrolysate, both of which have never been explored. This study aims to characterize and determine the in vitro antioxidant, antiglycation, and antityrosinase activity of pepsin-soluble collagen (PSC) and hydrolyzed collagen (HC) from mackerel scad skin. PSC was extracted using 0.5 M acetic acid containing 0.1% pepsin for 48 h at 4 °C. The obtained PSC was then hydrolyzed with collagenase type II (6250 U/g) to produce HC. The PSC yield obtained was 6.39 ± 0.97%, with a pH of 6.76 ± 0.18, while the HC yield was 96% from PSC. SDS-PAGE and Fourier Transform Infrared (FTIR) analysis showed the typical features of type I collagen. HC demonstrated high solubility (66.75–100%) throughout the entire pH range (1–10). The PSC and HC from mackerel scad skin showed antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), with IC50 values of 148.55 ± 3.14 ppm and 34.966 ± 0.518 ppm, respectively. In the antiglycation test, PSC had an IC50 value of 239.29 ± 15.67 ppm, while HC had an IC50 of 68.43 ± 0.44 ppm. PSC also exhibited antityrosinase activity, with IC50 values of 234.66 ± 0.185 ppm (on the L-DOPA substrate), while HC had an IC50 value of 79.35 ± 0.5 ppm. Taken together, these results suggest that the skin of mackerel scad fish has potential antiaging properties and can be further developed for pharmaceutical and cosmetic purposes.
Keywords: antiaging; antioxidant; collagen; hydrolyzed collagen; mackerel scad skin antiaging; antioxidant; collagen; hydrolyzed collagen; mackerel scad skin
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MDPI and ACS Style

Herawati, E.; Akhsanitaqwim, Y.; Agnesia, P.; Listyawati, S.; Pangastuti, A.; Ratriyanto, A. In Vitro Antioxidant and Antiaging Activities of Collagen and Its Hydrolysate from Mackerel Scad Skin (Decapterus macarellus). Mar. Drugs 2022, 20, 516. https://doi.org/10.3390/md20080516

AMA Style

Herawati E, Akhsanitaqwim Y, Agnesia P, Listyawati S, Pangastuti A, Ratriyanto A. In Vitro Antioxidant and Antiaging Activities of Collagen and Its Hydrolysate from Mackerel Scad Skin (Decapterus macarellus). Marine Drugs. 2022; 20(8):516. https://doi.org/10.3390/md20080516

Chicago/Turabian Style

Herawati, Elisa, Yochidamai Akhsanitaqwim, Pipin Agnesia, Shanti Listyawati, Artini Pangastuti, and Adi Ratriyanto. 2022. "In Vitro Antioxidant and Antiaging Activities of Collagen and Its Hydrolysate from Mackerel Scad Skin (Decapterus macarellus)" Marine Drugs 20, no. 8: 516. https://doi.org/10.3390/md20080516

APA Style

Herawati, E., Akhsanitaqwim, Y., Agnesia, P., Listyawati, S., Pangastuti, A., & Ratriyanto, A. (2022). In Vitro Antioxidant and Antiaging Activities of Collagen and Its Hydrolysate from Mackerel Scad Skin (Decapterus macarellus). Marine Drugs, 20(8), 516. https://doi.org/10.3390/md20080516

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