Preparation and Vasodilation Mechanism of Angiotensin-I-Converting Enzyme Inhibitory Peptide from Ulva prolifera Protein
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purification and Identification of ACE Inhibitory Peptides from U. prolifera Protein Hydrolysate
2.2. Screening of the Potential ACE Inhibitory Peptides
2.3. Inhibition Pattern of Peptide against ACE
2.4. Molecular Docking and Dynamics Simulation
2.5. Effect of Synthetic Peptides on NO Production, eNOS Activity in HUVECs
3. Materials and Methods
3.1. Materials and Reagents
3.2. Preparation of U. prolifera Protein Hydrolysate
3.3. Determination of ACE Inhibitory Activity
3.4. Purification and Identification of ACE-Inhibitory Peptides from Hydrolysates
3.5. Screening and Synthesis of the Potential ACE Inhibitory Peptides
3.6. Inhibition Kinetics
3.7. Molecular Dynamics (MD) Simulation
3.8. Cell Culture of HUVEC
3.9. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Peptide | Peptide Ranker | WS | Toxin | HIA | BBB | -C Dock Energy | IC50 (μM) |
---|---|---|---|---|---|---|---|
DRW | 0.88931 | GOOD | NO | 0.7047 | −0.8101 | fail | |
GMR | 0.884249 | GOOD | NO | 0.6327 | −0.6948 | fail | |
MGR | 0.860347 | GOOD | NO | 0.6327 | −0.6948 | fail | |
RYFR | 0.846704 | GOOD | NO | 0.4488 | 0.8791 | fail | |
KWY | 0.794787 | GOOD | NO | 0.9434 | −0.8790 | fail | |
RWK | 0.774831 | GOOD | NO | 0.8444 | −0.8040 | fail | |
LGSFR | 0.737369 | GOOD | NO | 0.8291 | −0.7994 | fail | |
EGRW | 0.734044 | GOOD | NO | 0.8291 | −0.7994 | fail | |
WRAA | 0.702731 | GOOD | NO | 0.9124 | −0.8063 | fail | |
KAF | 0.682106 | GOOD | NO | 0.7042 | −0.8181 | 88.967 | 0.63 ± 0.26 |
DFT | 0.600067 | GOOD | NO | 0.4747 | −0.8839 | fail | |
ERFY | 0.464208 | GOOD | NO | 0.6018 | −0.8872 | fail | |
PAMK | 0.419096 | GOOD | NO | 0.774 | −0.7314 | fail | |
Captopril | 46.94 | 0.017 [19] |
Candidates | Bond Position | Distance(Å) | Type | Number |
---|---|---|---|---|
KAF | A: ASP415:OD2-KAF:H22 A: ARG522:NH1-KAF: O44 A: ZN701: ZN-KAF: O44 | 2.16146 4.49144 2.24177 | Attractive Charge | 3 |
A: ASP415:OD1-KAF: H21 A: ALA354:O-KAF: H36 | 2.26404 2.3907 | Conventional Hydrogen Bond | 2 | |
A: HIS387:HD2-KAF: O44 A: TYR423: OH-KAF: H38 | 3.01083 2.3496 | Carbon Hydrogen Bond | 2 | |
A: VAL418-KAF | 4.90944 | Pi-Alkyl | 1 | |
A: ZN701: ZN-KAF: O34 | 3.0144 | Metal-Acceptor | 1 | |
Captopril (Cap) | A: ARG522:NH1-Cap: O11 A: ZN701: ZN-Cap: O11 | 4.49391 2.27274 | Attractive Charge | 2 |
A: ALA356:HN-Cap: O1 | 2.89368 | Conventional Hydrogen Bond | 1 | |
A: ALA354:O-Cap: H22 | 2.91634 | Carbon Hydrogen Bond | 1 | |
A: HIS387-Cap:H18 | 2.76629 | Pi-Donor Hydrogen Bond; Pi-Sulfur | 1 | |
A: VAL518-Cap | 4.64702 | Alkyl | 1 | |
A: PHE391-Cap: S4 A: HIS410-Cap: S4 | 4.77799 4.40347 | Pi-Sulfur | 2 |
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Li, Z.; He, H.; Liu, J.; Gu, H.; Fu, C.; Zeb, A.; Che, T.; Shen, S. Preparation and Vasodilation Mechanism of Angiotensin-I-Converting Enzyme Inhibitory Peptide from Ulva prolifera Protein. Mar. Drugs 2024, 22, 398. https://doi.org/10.3390/md22090398
Li Z, He H, Liu J, Gu H, Fu C, Zeb A, Che T, Shen S. Preparation and Vasodilation Mechanism of Angiotensin-I-Converting Enzyme Inhibitory Peptide from Ulva prolifera Protein. Marine Drugs. 2024; 22(9):398. https://doi.org/10.3390/md22090398
Chicago/Turabian StyleLi, Zhiyong, Hongyan He, Jiasi Liu, Huiyue Gu, Caiwei Fu, Aurang Zeb, Tuanjie Che, and Songdong Shen. 2024. "Preparation and Vasodilation Mechanism of Angiotensin-I-Converting Enzyme Inhibitory Peptide from Ulva prolifera Protein" Marine Drugs 22, no. 9: 398. https://doi.org/10.3390/md22090398