Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts
Abstract
:1. Introduction
2. Experimental Section
2.1. Collection of Water Samples
2.2. Culturing of Leptospira spp.
2.3. DNA Extraction
2.4. Quantitative Polymerase Chain Reaction (qPCR)
3. Results and Discussion
Source Type | Number of Samples Collected | Number Positive |
---|---|---|
Ponds | 15 | 0 |
Puddles | 13 | 1 |
Water dams | 8 | 5 |
Mountain springs | 5 | 2 |
Streams | 3 | 0 |
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Rawlins, J.; Portanova, A.; Zuckerman, I.; Loftis, A.; Ceccato, P.; Willingham, A.L.; Verma, A. Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts. Int. J. Environ. Res. Public Health 2014, 11, 7953-7960. https://doi.org/10.3390/ijerph110807953
Rawlins J, Portanova A, Zuckerman I, Loftis A, Ceccato P, Willingham AL, Verma A. Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts. International Journal of Environmental Research and Public Health. 2014; 11(8):7953-7960. https://doi.org/10.3390/ijerph110807953
Chicago/Turabian StyleRawlins, Julienne, Alexandra Portanova, Ilana Zuckerman, Amanda Loftis, Pietro Ceccato, Arve Lee Willingham, and Ashutosh Verma. 2014. "Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts" International Journal of Environmental Research and Public Health 11, no. 8: 7953-7960. https://doi.org/10.3390/ijerph110807953