Two-Year Monitoring of Water Samples from Dam of Iskar and the Black Sea, Bulgaria, by Molecular Analysis: Focus on Mycobacterium spp.
Abstract
:1. Introduction
2. Experimental Section
2.1. Sampling
2.2. Bacterial and Viral Detection
3. Results and Discussion
Target microorganism | Black Sea (n = 14) | Dam of Iskar (n = 24) |
---|---|---|
Mycobacterium spp. | 0 | 0 |
Vibirio spp. | 6 | 0 |
Listeria monocytogenes | 0 | 0 |
Campilobacter spp. | 0 | 0 |
Pseudomonas spp. | 1 | 0 |
Shigella spp. | 0 | 0 |
Salmonella spp. | 0 | 0 |
Legionella spp. | 0 | 1 |
Yersinia enterocolitica | 0 | 0 |
Clostridium perfringens | 0 | 0 |
Clostridium botulinum | 0 | 0 |
E. coli EHEC stx1 and stx2 | 3 | 4 |
Staphylococcus aureus | 0 | 0 |
Aeromonas spp. | 0 | 0 |
Rotavirus A | 0 | 4 |
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Appendix
№/Date | Location/GPS coordinates | Yersinia enterocolitica | Vibirio spp | Listeria monocytogenes | Mycobactrium spp. | Salmonella spp | Shigella spp. | Campilobacter spp. | E. coli EHEC stx1 and stx2 | Legionella spp. | Clostridium perfringens | Clostridium botulinum | S. aureus | Aeromonas spp | P.aeruginosa | Rotavirus A |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1. 16.01.2013 | Dam of Iskar—Zlo kuche | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA | |
2. 09.06.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | |
3. 16.06.2013 | Dam of Iskar—Zlokuche | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | |
4. 22.06.2013 | Dam of Iskar—Zlokuche | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | ||
5. 29.06.2013 | Dam of Iskar—Zlokuche | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG |
6. 14.07.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
7. 21.07.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG |
8. 28.07.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
9. 04.08.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
10. 18.08.2013 | Dam of Iskar–Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
11. 15.09.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
12. 23.09.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA | |
13. 29.09.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA | |
14. 04.10.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG | |
15. 13.10.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG |
16. 20.10.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
17. 27.10.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
18. 03.11.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
19. 06.11.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
20. 10.11.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
21. 17.11.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
22. 24.11.2013 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
23. 12.05.14 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | |
24. 30.06.14 | Dam of Iskar—Shtarkelovo gnezdo | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | ||
25. 09.09.2012 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA | |
26. 16.09.2012 | Black Sea—Ahtopol | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | ||
27. 23.09.2012 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG | |
28. 12.01.2013 | Black Sea—Sozopol | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA | |
29. 01.05.2013 | Black Sea – Ahtopol | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA | |
30. 23.06.2013 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
31. 20.07.2013 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA | |
32. 31.08.2013 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
33. 01.09.2013 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG | |
34. 10.10.2013 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NA |
35. 14.04.2014 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG | |
36. 25.07.2014 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG |
37. 25.08.2014. | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG |
38. 05.09.2014 | Black Sea—Rezovo village | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NEG | NA | NEG |
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Panaiotov, S.; Simeonovski, I.; Levterova, V.; Karamfilov, V.; Brankova, N.; Tankova, K.; Campbell, K.; Jacob, P.; Helmi, K.; Boots, B.; et al. Two-Year Monitoring of Water Samples from Dam of Iskar and the Black Sea, Bulgaria, by Molecular Analysis: Focus on Mycobacterium spp. Int. J. Environ. Res. Public Health 2015, 12, 7430-7443. https://doi.org/10.3390/ijerph120707430
Panaiotov S, Simeonovski I, Levterova V, Karamfilov V, Brankova N, Tankova K, Campbell K, Jacob P, Helmi K, Boots B, et al. Two-Year Monitoring of Water Samples from Dam of Iskar and the Black Sea, Bulgaria, by Molecular Analysis: Focus on Mycobacterium spp. International Journal of Environmental Research and Public Health. 2015; 12(7):7430-7443. https://doi.org/10.3390/ijerph120707430
Chicago/Turabian StylePanaiotov, Stefan, Ivan Simeonovski, Victoria Levterova, Ventzislav Karamfilov, Nadia Brankova, Kristin Tankova, Katrina Campbell, Pauline Jacob, Karim Helmi, Bas Boots, and et al. 2015. "Two-Year Monitoring of Water Samples from Dam of Iskar and the Black Sea, Bulgaria, by Molecular Analysis: Focus on Mycobacterium spp." International Journal of Environmental Research and Public Health 12, no. 7: 7430-7443. https://doi.org/10.3390/ijerph120707430