DNA Damage in Euonymus japonicus Leaf Cells Caused by Roadside Pollution in Beijing
Abstract
:1. Introduction
2. Experimental Section
2.1. Test Samples
2.2. Chemicals and Media
2.3. Comet Assay
3. Results and Discussion
3.1. Results of DNA Damage Assay
3.1.1. Tail DNA Percent Analysis
3.1.2. Tail Moment Analysis
3.2. Spatial Distribution Analysis of Dust Pollution
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
- Risom, L.; Møller, P.; Loft, S. Oxidative stress-induced DNA damage by particulate air pollution. Mutat. Res. 2005, 592, 119–137. [Google Scholar] [CrossRef] [PubMed]
- Novotna, B.; Topinka, J.; Solansky, I.; Chvatalova, I.; Lnenickova, Z.; Sram, R.J. Impact of air pollution and genotype variability on DNA damage in Prague policemen. Toxicol. Lett. 2007, 172, 37–47. [Google Scholar] [CrossRef] [PubMed]
- Qin, W.; ChunYu, X.; DongQun, X. Polycyclic aromatic hydrocarbons exposure and DNA oxidative damage in traffic policemen of Beijing. J. Environ. Health 2009, 26, 770–773. [Google Scholar]
- Maselli, V.; Polese, G.; Rippa, D.; Ligrone, R.; Rastog, R.K.; Fulgiome, D. Frogs, sentinels of DNA damage induced by pollution in Naples and the neighbouring Provinces. Ecotoxicol. Environ. Saf. 2010, 73, 1525–1529. [Google Scholar] [CrossRef] [PubMed]
- Hart, J.E.; Liao, X.; Hong, B.; Puett, R.C.; Yanosky, J.D.; Suh, H.; Kioumourtzoglou, M.; Spiegelman, D.; Laden, F. The association of long-term exposure to PM2.5 on all-cause mortality in the Nurses’ Health Study and the impact of measurement-error correction. Environ. Health 2015, 14, 1–9. [Google Scholar] [CrossRef] [PubMed]
- Barbato, D.L.; Tomei, G.; Tomei, F.; Sancini, A. Traffic air pollution and oxidatively generated DNA damage: Can urinary 8-oxo-7,8-dihydro-2-deoxiguanosine be considered a good biomarker A meta-analysis. Biomarkers 2010, 15, 538–545. [Google Scholar] [CrossRef] [PubMed]
- Møller, P.; Danielsen, P.H.; Karottki, D.G.; Jantzen, K.; Roursgaard, M.; Klingberg, H.; Jensen, D.M.; Christophersen, D.V.; Hemmingsen, J.G.; Cao, Y.; et al. Oxidative stress and inflammation generated DNA damage by exposure to air pollution particles. Press. Med. 2014, 762, 133–166. [Google Scholar] [CrossRef] [PubMed]
- Sriussadaporn, C.; Yamamoto, K.; Fukushi, K.; Simazaki, D. Comparison of DNA damage detected by plant comet assay in roadside and non-roadside environments. Mutat. Res. 2003, 541, 31–44. [Google Scholar] [CrossRef]
- Lin, A.J.; Zhang, X.H.; Zhu, Y.G. The comet assay detects Cd-induced DNA damages in wheat leaves. Acta Sci. Circumst. 2005, 25, 329–333. [Google Scholar]
- Ojima, Y.; Nishioka, M.; Matsumoto, M.; Taya, M. Quantification of DNA damage by the comet assay in radish sprouts exposed to excess light irradiation. Biochem. Eng. J. 2009, 46, 69–72. [Google Scholar] [CrossRef]
- Hattab, S.; Chouba, L.; Kheder, M.B.; Mahouachi, T.; Boussetta, H. Cadmium- and copper-induced DNA damage in Pisum sativum, roots and leaves as determined by the Comet assay. Plant Biosyst. 2009, 143, S6–S11. [Google Scholar] [CrossRef]
- Ostling, O.; Johanson, K.J. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 1984, 123, 291–298. [Google Scholar] [CrossRef]
- Yasir, F.; Naila, S.; Muhammad, T. Pollution level and health risk assessment of road dust from an expressway. J. Environ. Sci. Health Part A Tox. Hazard. Subst. Environ. Eng. 2012, 47, 818–829. [Google Scholar]
- Cortes-Gutierrez, E.I.; Dávila-Rodriguez, M.I.; Fernández, J.L.; López-Fernández, C.; Gosálbez, A.; Gosálvez, J. New Application of the Comet Assay: Chromosome–Comet Assay. J. Histochem. Cytochem. 2011, 59, 655–660. [Google Scholar] [CrossRef] [PubMed]
- Singh, N.P.; Mccoy, M.T.; Tice, R.R.; Schneider, E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 1988, 175, 184–191. [Google Scholar] [CrossRef]
- Miranda-Vilela, A.L.; Alves, P.C.; Akimoto, A.K.; Lordelo, G.S.; Goncalves, C.A.; Grisolia, K.; Klautau-Guimaraes, M.N. Gene polymorphisms against DNA damage induced by hydrogen peroxide in leukocytes of healthy humans through comet assay: A quasi-experimental study. Environ. Health 2010, 9, 21. [Google Scholar] [CrossRef] [PubMed]
- Gichner, T. DNA damage induced by indirect and direct acting mutagens in catalase-deficient transgenic tobacco: Cellular and acellular Comet assays. Mutat. Res. 2003, 535, 187–193. [Google Scholar] [CrossRef]
- Peycheva, E.; Ivanova, E.; Ivanov, S.; Miloshev, G. Improved procedure for Comet assay on active photosynthetic cells from pea plants. Oxid. Commun. 2011, 34, 320–325. [Google Scholar]
- Collins, A.R.; Oscoz, A.A.; Brunborg, G.; Gaivao, I.; Giovannelli, L.; Kruszewski, M.; Smith, C.C.; Štětina, R. The comet assay: Topical issues. Mutagenesis 2008, 23, 143–151. [Google Scholar] [CrossRef] [PubMed]
- Pourrut, B.; Jean, S.; Silvestre, J.; Pinelli, E. Lead-induced DNA damage in Vicia faba root cells: Potential involvement of oxidative stress. Mutat. Res./Genet. Toxicol. Environ. Mutagen. 2011, 726, 123–128. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Clara, E.; Lennart, M. The effects on DNA migration of altering parameters in the comet assay protocol such as agarose density, electrophoresis conditions and durations of the enzyme or the alkaline treatments. Mutagenesis 2011, 26, 689–695. [Google Scholar]
- Amaya, A.; Collins, A.R. The essential comet assay: A comprehensive guide to measuring DNA damage and repair. Arch. Toxicol. 2013, 87, 949–968. [Google Scholar]
- Bonacci, S.; Iacocca, A.; Lancini, L. DNA adducts, benzo(a)pyrene monooxygenase activity, and lysosomal membrane stability in Mytilus galloprovincialis from different areas in Taranto coastal waters (Italy). Environ. Res. 2004, 96, 163–175. [Google Scholar]
- Pan, L.; Liu, N.; Zhang, H.; Wang, J.; Miao, J. Effects of Heavy Metal Ions (Cu2+, Pb2+ and Cd2+) on DNA Damage of the Gills, Hemocytes and Hepatopancreas of Marine Crab, Charybdis japonica. J. Ocean Univ. China 2011, 10, 177–184. [Google Scholar] [CrossRef]
- Olive, P.L. DNA damage and repair in individual cells: Applications of the comet assay in radiobiology. Int. J. Radiat. Biol. 1999, 75, 395–405. [Google Scholar] [CrossRef] [PubMed]
- Marco, S.G.; Zheng, Y.; Citlalli, O.Y.; Zhong, J.; Chervona, Y.; Wang, S.; Chang, D.; McCracken, J.P.; Díaz, A.; Bertazzi, P.A.; et al. Effects of particulate matter exposure on blood 5-hydroxymethylation: Results from the Beijing truck driver air pollution study. Epigenetics 2015, 10, 633–642. [Google Scholar]
- Huang, H.B.; Lai, C.H.; Chen, G.W.; Lin, Y.Y.; Jaakkola, J.J.; Liou, S.H.; Wang, S.L. Traffic-Related Air Pollution and DNA Damage: A Longitudinal Study in Taiwanese Traffic Conductors. PLoS ONE 2012, 7, e37412. [Google Scholar] [CrossRef] [PubMed]
- Tovalin, H.; Valverde, M.; Morandi, M.T.; Blanco, S.; Whitehead, L.; Rojas, E. DNA damage in outdoor workers occupationally exposed to environmental air pollutants. Occup. Environ. Med. 2006, 63, 230–236. [Google Scholar] [CrossRef] [PubMed]
Leaf Ages | Beishan | Xueyuan Bridge | Jimen Bridge | Beijing Northern Rail Station | Beijing Western Rail Station |
---|---|---|---|---|---|
One year | 39.5 ± 1.2 | 93.5 ± 1.6 | 106.8 ± 1.3 | 150 ± 1.4 | 137 ± 2.0 |
Two years | 53.3 ± 1.8 | 104.3 ± 1.7 | 134.9 ± 2.2 | 163.3 ± 2.4 | 158.8 ± 1.8 |
Three years | 59 ± 1.6 | 110.2 ± 2.0 | 144.6 ± 2.0 | 178.7 ± 2.2 | 167.8 ± 2.2 |
Sampling Sites | Fine AQI | Poor AQI | ||
---|---|---|---|---|
PM2.5 | PM10 | PM2.5 | PM10 | |
Beishan | 21 | 24 | 87 | 93 |
Xueyuan Bridge | 53 | 57 | 275 | 291 |
Jimen Bridge | 52 | 55 | 276 | 293 |
Beijing Northern Rail Station | 74 | 81 | 313 | 325 |
Beijing Western Rail Station | 58 | 63 | 282 | 298 |
Sampling Sites | Beishan | Xueyuan Bridge | Jimen Bridge | Beijing Northern Rail Station | Beijing Western Rail Station |
---|---|---|---|---|---|
AQI | 72 | 171 | 172 | 183 | 174 |
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Li, T.; Zhang, M.; Gu, K.; Herman, U.; Crittenden, J.; Lu, Z. DNA Damage in Euonymus japonicus Leaf Cells Caused by Roadside Pollution in Beijing. Int. J. Environ. Res. Public Health 2016, 13, 742. https://doi.org/10.3390/ijerph13070742
Li T, Zhang M, Gu K, Herman U, Crittenden J, Lu Z. DNA Damage in Euonymus japonicus Leaf Cells Caused by Roadside Pollution in Beijing. International Journal of Environmental Research and Public Health. 2016; 13(7):742. https://doi.org/10.3390/ijerph13070742
Chicago/Turabian StyleLi, Tianxin, Minjie Zhang, Ke Gu, Uwizeyimana Herman, John Crittenden, and Zhongming Lu. 2016. "DNA Damage in Euonymus japonicus Leaf Cells Caused by Roadside Pollution in Beijing" International Journal of Environmental Research and Public Health 13, no. 7: 742. https://doi.org/10.3390/ijerph13070742