LRBA Gene Polymorphisms and Risk of Coal Workers’ Pneumoconiosis: A Case–Control Study from China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Subjects
2.2. SNP Selection
2.3. Genotyping
2.4. Bioinformatical Analysis and Gene Expression Levels
2.5. Statistical Analysis
3. Results
3.1. Characteristics of the Study Subjects
3.2. Associations between the LRBA Polymorphisms and Risk of CWP
3.3. Potential Biological Roles of LRBA rs2290846 in CWP
4. Discussion
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Variables | CWP (n = 703) | Controls (n = 705) | p | ||
---|---|---|---|---|---|
n | % | n | % | ||
Age, years (mean ± SD) | 67.3 ± 10.8 | 66.3 ± 9.7 | 0.086 | ||
Exposure years (mean ± SD) | 24.9 ± 8.5 | 25.5 ± 7.1 | 0.170 | ||
Smoking | 0.088 | ||||
No | 374 | 53.2 | 343 | 48.7 | |
Yes | 329 | 46.8 | 362 | 51.3 | |
Pack-years smoked | <0.001 | ||||
0 | 374 | 53.2 | 343 | 48.7 | |
0–20 | 201 | 28.6 | 165 | 23.4 | |
>20 | 128 | 18.2 | 197 | 27.9 | |
Job type | 0.703 | ||||
Tunnel and coal mining | 590 | 83.9 | 599 | 85.0 | |
Transport | 40 | 5.7 | 42 | 6.0 | |
Others | 73 | 10.4 | 64 | 9.1 | |
Stage | |||||
I | 431 | 61.3 | |||
II | 209 | 29.7 | |||
III | 63 | 9.0 |
Cluster ID | Region | dbSNP Allele | Function | Protein Residue | MAF | HWE a | |
---|---|---|---|---|---|---|---|
Case | Control | ||||||
rs2290846 | Exon-56/57 | G > A | missense | Ser/Leu | 0.256 | 0.279 | 0.060 |
rs3749574 | Exon-54/57 | C > T | missense | Ala/Thr | 0.251 | 0.275 | 0.504 |
rs1782360 | Exon-54/57 | G > C | missense | Ala/Gly | 0.125 | 0.123 | 0.091 |
Variables | CWP Cases | Controls | OR (95% CI) a | p a | OR (95% CI) b | p b | ||
---|---|---|---|---|---|---|---|---|
n | % | n | % | |||||
rs2290846 | n = 695 | n = 684 | ||||||
GG | 384 | 55.3 | 371 | 54.2 | 1.00 | - | 1.00 | - |
GA | 266 | 38.3 | 245 | 35.8 | 1.05 (0.84–1.31) | 0.677 | 1.05 (0.84–1.32) | 0.680 |
AA | 45 | 6.5 | 68 | 9.9 | 0.64 (0.43–0.96) | 0.030 | 0.61 (0.41–0.92) | 0.018 |
Dominant | 0.96 (0.78–1.19) | 0.706 | 0.95 (0.77–1.18) | 0.658 | ||||
Recessive | 0.63 (0.42–0.93) | 0.020 | 0.60 (0.40–0.89) | 0.011 | ||||
Additive | 0.90 (0.76–1.06) | 0.196 | 0.89 (0.75–1.05) | 0.152 | ||||
rs3749574 | n = 698 | n = 692 | ||||||
CC | 386 | 55.3 | 360 | 52.0 | 1.00 | - | 1.00 | - |
CT | 274 | 39.3 | 284 | 41.0 | 0.90 (0.72–1.12) | 0.346 | 0.90 (0.72–1.13) | 0.364 |
TT | 38 | 5.4 | 48 | 6.9 | 0.74 (0.47–1.16) | 0.186 | 0.70 (0.45–1.10) | 0.124 |
Dominant | 0.88 (0.71–1.08) | 0.221 | 0.88 (0.71–1.09) | 0.232 | ||||
Recessive | 0.77 (0.50–1.20) | 0.249 | 0.73 (0.47–1.14) | 0.162 | ||||
Additive | 0.88 (0.74–1.05) | 0.146 | 0.87 (0.73–1.04) | 0.127 | ||||
rs1782360 | n = 695 | n = 686 | ||||||
GG | 539 | 77.6 | 534 | 77.8 | 1.00 | - | 1.00 | - |
CG | 139 | 20.0 | 135 | 19.7 | 1.02 (0.78–1.33) | 0.883 | 1.01 (0.77–1.32) | 0.943 |
CC | 17 | 2.4 | 17 | 2.5 | 0.99 (0.50–1.96) | 0.979 | 1.01 (0.51–2.02) | 0.971 |
Dominant | 1.02 (0.79–1.31) | 0.898 | 1.01 (0.78–1.30) | 0.964 | ||||
Recessive | 0.99 (0.50–1.95) | 0.969 | 1.01 (0.51–2.00) | 0.985 | ||||
Additive | 1.01 (0.81–1.26) | 0.922 | 1.01 (0.81–1.25) | 0.964 |
Variables | rs2290846 | |||
---|---|---|---|---|
Cases a | Controls a | OR (95% CI) b | p b | |
Exposure years | ||||
<27 | 341/27 | 355/37 | 0.73 (0.43–1.23) | 0.237 |
≥27 | 309/18 | 261/31 | 0.51 (0.28–0.94) | 0.032 |
Pack-years smoked | ||||
0 | 343/24 | 302/36 | 0.58 (0.34–1.00) | 0.049 |
>0–20 | 184/16 | 138/19 | 0.65 (0.32–1.33) | 0.241 |
>20 | 123/5 | 176/13 | 0.57 (0.20–1.69) | 0.313 |
Variables | Cases/Controls | Genotypes (Cases/Controls) | p a | OR (95% CI) a | |||
---|---|---|---|---|---|---|---|
AA | GG/GA | ||||||
N | % | N | % | ||||
Total | 703/705 | 45/68 | 6.4/9.6 | 658/637 | 93.6/90.4 | 0.011 | 0.60 (0.40–0.89) |
Stage | |||||||
Ι | 431/705 | 26/68 | 6.0/9.6 | 405/637 | 94.0/90.4 | 0.035 | 0.60 (0.37–0.97) |
II | 209/705 | 14/68 | 6.7/9.6 | 195/637 | 93.3/90.4 | 0.137 | 0.62 (0.34–1.16) |
III | 63/705 | 5/68 | 7.9/9.6 | 58/637 | 92.1/90.4 | 0.381 | 0.65 (0.24–1.72) |
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Liu, Y.; Yang, J.; Wu, Q.; Han, R.; Yan, W.; Yuan, J.; Ji, X.; Li, Y.; Yao, W.; Ni, C. LRBA Gene Polymorphisms and Risk of Coal Workers’ Pneumoconiosis: A Case–Control Study from China. Int. J. Environ. Res. Public Health 2017, 14, 1138. https://doi.org/10.3390/ijerph14101138
Liu Y, Yang J, Wu Q, Han R, Yan W, Yuan J, Ji X, Li Y, Yao W, Ni C. LRBA Gene Polymorphisms and Risk of Coal Workers’ Pneumoconiosis: A Case–Control Study from China. International Journal of Environmental Research and Public Health. 2017; 14(10):1138. https://doi.org/10.3390/ijerph14101138
Chicago/Turabian StyleLiu, Yi, Jingjin Yang, Qiuyun Wu, Ruhui Han, Weiwen Yan, Jiali Yuan, Xiaoming Ji, Yan Li, Wenxi Yao, and Chunhui Ni. 2017. "LRBA Gene Polymorphisms and Risk of Coal Workers’ Pneumoconiosis: A Case–Control Study from China" International Journal of Environmental Research and Public Health 14, no. 10: 1138. https://doi.org/10.3390/ijerph14101138