Treatment of Human Placental Choriocarcinoma Cells with Formaldehyde and Benzene Induced Growth and Epithelial Mesenchymal Transition via Induction of an Antioxidant Effect
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Chemicals
2.2. Cell Culture and Media
2.3. MTT Assay
2.4. Scratch Assay
2.5. Protein Extraction and Western Blot Assay
2.6. Determination of ROS Production
2.7. Data Analysis
3. Results
3.1. FA and Bz Induced Increased Cell Proliferation
3.2. Effects of CS Components on Protein Expression of Cell Cycle Regulatory Genes
3.3. FA and Bz Induced Activation of Migration in JEG-3
3.4. Effects of CS Components on Protein Expression of EMT Progress Genes
3.5. FA and Bz Activated ROS Synthesis and Increased Antioxidant Factor
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lee, H.-M.; Kim, S.-M.; Choi, K.-C. Treatment of Human Placental Choriocarcinoma Cells with Formaldehyde and Benzene Induced Growth and Epithelial Mesenchymal Transition via Induction of an Antioxidant Effect. Int. J. Environ. Res. Public Health 2017, 14, 854. https://doi.org/10.3390/ijerph14080854
Lee H-M, Kim S-M, Choi K-C. Treatment of Human Placental Choriocarcinoma Cells with Formaldehyde and Benzene Induced Growth and Epithelial Mesenchymal Transition via Induction of an Antioxidant Effect. International Journal of Environmental Research and Public Health. 2017; 14(8):854. https://doi.org/10.3390/ijerph14080854
Chicago/Turabian StyleLee, Hae-Miru, Soo-Min Kim, and Kyung-Chul Choi. 2017. "Treatment of Human Placental Choriocarcinoma Cells with Formaldehyde and Benzene Induced Growth and Epithelial Mesenchymal Transition via Induction of an Antioxidant Effect" International Journal of Environmental Research and Public Health 14, no. 8: 854. https://doi.org/10.3390/ijerph14080854
APA StyleLee, H. -M., Kim, S. -M., & Choi, K. -C. (2017). Treatment of Human Placental Choriocarcinoma Cells with Formaldehyde and Benzene Induced Growth and Epithelial Mesenchymal Transition via Induction of an Antioxidant Effect. International Journal of Environmental Research and Public Health, 14(8), 854. https://doi.org/10.3390/ijerph14080854