Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Analysis of Sperm Count and Sperm Abnormality Rate
2.3. Histological Change in the Testis
2.4. Biochemical Assays
2.5. Western Blot Analysis
2.6. Statistical Analysis
3. Results
3.1. Effect of Perinatal Bisphenol A Exposurure on Body Weight and Food, Water Intake in Maternal Mice and Their Offspring
3.2. The Effect of Perinatal Bisphenol A Exposure on Sperm Production Capacity in Male Offspring
3.3. The Effect of Perinatal Exposure to Bisphenol A on Levels of Serum Testosterone and Tumor Necrosis Factor-α in Male Offspring
3.4. The Effect of Perinatal Exposure to Bisphenol A on Inflammatory Factor-Related Protein Expression in Testis Tissue from Male Offspring
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Group | Testis | Epididymis | ||
---|---|---|---|---|
Weight (g) | Coefficient (%) | Weight (g) | Coefficient (%) | |
Control | 0.15 ± 0.03 | 0.65 ± 0.06 | 0.08 ± 0.02 | 0.37 ± 0.03 |
LBPA | 0.16 ± 0.02 | 0.70 ± 0.09 | 0.09 ± 0.01 | 0.39 ± 0.05 |
HBPA | 0.16 ± 0.03 | 0.68 ± 0.08 | 0.08 ± 0.01 | 0.35 ± 0.04 |
Group | n | Testosterone (ng/mL) | TNF-α (ng/L) |
---|---|---|---|
Control | 7 | 13.47 ± 1.12 | 3.31 ± 0.58 |
LBPA | 7 | 12.93 ± 0.98 | 4.26 ± 0.35 * |
HBPA | 7 | 10.69 ± 1.04 ** | 4.46 ± 0.37 * |
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Meng, Y.; Lin, R.; Wu, F.; Sun, Q.; Jia, L. Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice. Int. J. Environ. Res. Public Health 2018, 15, 2158. https://doi.org/10.3390/ijerph15102158
Meng Y, Lin R, Wu F, Sun Q, Jia L. Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice. International Journal of Environmental Research and Public Health. 2018; 15(10):2158. https://doi.org/10.3390/ijerph15102158
Chicago/Turabian StyleMeng, Yuan, Ren Lin, Fengjuan Wu, Qi Sun, and Lihong Jia. 2018. "Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice" International Journal of Environmental Research and Public Health 15, no. 10: 2158. https://doi.org/10.3390/ijerph15102158