The Nitrogen-Removal Efficiency of a Novel High-Efficiency Salt-Tolerant Aerobic Denitrifier, Halomonas Alkaliphile HRL-9, Isolated from a Seawater Biofilter
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling and Media
2.2. Strain Isolation
2.3. SEM Analysis and Identification of Strain HRL-9
2.4. Single-Factor Affecting Aerobic Denitrification
2.5. Estimation of Aerobic Denitrification Ability of Strain HRL-9
2.6. Nitrogen Balance Analysis
2.7. Amplification of napA and narG Gene
2.8. Analytical Methods
3. Results
3.1. Identification of Strain HRL-9
3.2. The Influence of Shaking Speed, Temperature and C/N Ratio on Aerobic Denitrification
3.2.1. Effect of Shaking Speed
3.2.2. Effect of Temperature
3.2.3. Effect of C/N
3.3. Aerobic Denitrification and Nitrogen Balance of Strain HRL-9
3.4. Amplification of napA and narG Gene
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Substance | Initial TN mg·L−1 | Final TN mg·L−1 | Intracellular-N/% | Nitrogen Remove/% | |||
---|---|---|---|---|---|---|---|
NO3−-N | NO2−-N | NH4+-N | Organic-N | ||||
Nitrate | 101.4 ± 0.02 | 1.24 ± 0.01 | 0.10 ± 0.01 | 0.50 ± 0.10 | 2.75 ± 0.32 | 21.7 ± 0.28 | ≈74.5 |
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Ren, J.; Wei, C.; Ma, H.; Dai, M.; Fan, J.; Liu, Y.; Wu, Y.; Han, R. The Nitrogen-Removal Efficiency of a Novel High-Efficiency Salt-Tolerant Aerobic Denitrifier, Halomonas Alkaliphile HRL-9, Isolated from a Seawater Biofilter. Int. J. Environ. Res. Public Health 2019, 16, 4451. https://doi.org/10.3390/ijerph16224451
Ren J, Wei C, Ma H, Dai M, Fan J, Liu Y, Wu Y, Han R. The Nitrogen-Removal Efficiency of a Novel High-Efficiency Salt-Tolerant Aerobic Denitrifier, Halomonas Alkaliphile HRL-9, Isolated from a Seawater Biofilter. International Journal of Environmental Research and Public Health. 2019; 16(22):4451. https://doi.org/10.3390/ijerph16224451
Chicago/Turabian StyleRen, Jilong, Chenzheng Wei, Hongjing Ma, Mingyun Dai, Jize Fan, Ying Liu, Yinghai Wu, and Rui Han. 2019. "The Nitrogen-Removal Efficiency of a Novel High-Efficiency Salt-Tolerant Aerobic Denitrifier, Halomonas Alkaliphile HRL-9, Isolated from a Seawater Biofilter" International Journal of Environmental Research and Public Health 16, no. 22: 4451. https://doi.org/10.3390/ijerph16224451