Effect of Polyols and Selected Dental Materials on the Ability to Create a Cariogenic Biofilm–On Children Caries-Associated Streptococcus Mutans Isolates
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical Aspects
2.2. Participants of the Study
2.3. Dental Plaque Collection
2.4. Laboratory Analyses and Proceeding with Dental Materials
2.5. Microbiological Analysis
2.6. In Vitro Biofilm Formation
2.7. In Vitro Biofilm Model
2.8. Statistical Analysis
3. Results
3.1. Microbiological Identification
3.2. Surface of Tested Materials
3.3. Biofilm Biomass Assessment
3.4. Measurement of Total Biomass Expressed as OD (Optical Density)
3.5. Determination of the Amount of Live Microorganisms in a Biofilm
3.6. Morphological Characteristics of Biofilms
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
References
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Material | 5% Sucrose | 5% Xylitol | 5% Sorbitol | 5% Erythritol |
---|---|---|---|---|
Control | 0.172 (0.030) a | 0.154 (0.012) a | 0.155 (0.010) a | 0.149 (0.011) a |
Composite | 0.321 (0.031) a | 0.271 (0.033) a | 0.256 (0.023) a | 0.247 (0.014) b |
Glass ionomer | 1.321 (0.024) a | 1.121 (0.025) a | 0.967 (0.018) a | 0.887 (0.053) b |
Character | Sucrose | Xylitol | Sorbitol | Erythritol | (ANOVA) p |
---|---|---|---|---|---|
Time | OD540 mean ± SD (median ± QD) | ||||
12 h | 0.11 ± 0.01 (0.11 ± 0.01) | 0.10 ± 0.01 ** (0.11 ± 0.01) | 0.10 ± 0.01 **** (0.10 ± 0.01) | 0.09 ± 0.01 **** (0.09 ± 0.01) | <0.0001 |
24 h | 0.12 ± 0.01 (0.12 ± 0.00) | 0.11 ± 0.02 (0.12 ± 0.01) | 0.10 ± 0.01 *** (0.11 ± 0.01) | 0.08 ± 0.01 **** (0.08 ± 0.01) | <0.0001 |
48 h | 0.12 ± 0.01 (0.12 ± 0.01) | 0.11 ± 0.02 (0.12 ± 0.01) | 0.11 ± 0.01 ** (0.11 ± 0.01) | 0.08 ± 0.01 **** (0.08 ± 0.00) | <0.0001 |
72 h | 0.14 ± 0.01 (0.14 ± 0.01) | 0.12 ± 0.01 **** (0.13 ± 0.01) | 0.11 ± 0.01 **** (0.12 ± 0.01) | 0.08 ± 0.01 **** (0.08 ± 0.01) | <0.0001 |
Log(CFU/mL) mean ± SD (median ± QD) | |||||
12 h | 1.32 ± 0.05 (1.32 ± 0.05) | 1.12 ± 0.07 *** (1.13 ± 0.06) | 0.92 ± 0.10 *** (0.93 ± 0.05) | 0.85 ± 0.12 *** (0.85 ± 0.08) | <0.0001 |
24 h | 1.34 ± 0.04 (1.34 ± 0.02) | 1.22 ± 0.05 *** (1.22 ± 0.03) | 1.14 ± 0.09 *** (1.15 ± 0.08) | 0.86 ± 0.22 *** (0.90 ± 0.12) | <0.0001 |
48 h | 1.51 ± 0.16 (1.48 ± 0.09) | 1.26 ± 0.06 *** (1.25 ± 0.03) | 1.24 ± 0.07 *** (1.24 ± 0.04) | 0.84 ± 0.21 *** (0.85 ± 0.08) | <0.0001 |
72 h | 1.70 ± 0.07 (1.71 ± 0.03) | 1.36 ± 0.06 *** (1.35 ± 0.04) | 1.31 ± 0.07 *** (1.30 ± 0.04) | 0.84 ± 0.10 *** (0.85 ± 0.06) | <0.0001 |
Character | Sucrose | Xylitol | Sorbitol | Erythritol | (ANOVA) p |
---|---|---|---|---|---|
Time | OD540 mean ± SD (median ± QD) | ||||
12 h | 0.08 ± 0.01 (0.08 ± 0.01) | 0.07 ± 0.01 (0.07 ± 0.01) | 0.06 ± 0.01 **** (0.06 ± 0.01) | 0.06 ± 0.01 **** (0.06 ± 0.00) | <0.0001 |
24 h | 0.08 ± 0.00 (0.08 ± 0.00) | 0.07 ± 0.01 ** (0.07 ± 0.01) | 0.06 ± 0.01 **** (0.06 ± 0.01) | 0.06 ± 0.01 **** (0.06 ± 0.00) | <0.0001 |
48 h | 0.08 ± 0.01 (0.08 ± 0.00) | 0.07 ± 0.01 (0.07 ± 0.01) | 0.06 ± 0.01 **** (0.06 ± 0.01) | 0.06 ± 0.01 **** (0.05 ± 0.01) | <0.0001 |
72 h | 0.08 ± 0.00 (0.08 ± 0.00) | 0.08 ± 0.01 (0.08 ± 0.01) | 0.06 ± 0.01 **** (0.06 ± 0.01) | 0.05 ± 0.01 **** (0.05 ± 0.01) | <0.0001 |
Log(CFU/mL) mean ± SD (median ± QD) | |||||
12 h | 1.26 ± 0.09 (1.26 ± 0.05) | 1.31 ± 0.09 (1.31 ± 0.05) | 0.97 ± 0.16 **** (0.95 ± 0.13) | 0.83 ± 0.13 **** (0.84 ± 0.10) | <0.0001 |
24 h | 1.27 ± 0.06 (1.26 ± 0.05) | 1.33 ± 0.08 (1.34 ± 0.05) | 1.10 ± 0.15 **** (1.15 ± 0.15) | 0.80 ± 0.11 **** (0.81 ± 0.09) | <0.0001 |
48 h | 1.28 ± 0.05 (1.29 ± 0.03) | 1.32 ± 0.07 (1.32 ± 0.05) | 1.17 ± 0.12 **** (1.20 ± 0.10) | 0.77 ± 0.12 **** (0.75 ± 0.09) | <0.0001 |
72 h | 1.28 ± 0.05 (1.29 ± 0.03) | 1.35 ± 0.06 ** (1.36 ± 0.05) | 1.15 ± 0.11 **** (1.18 ± 0.08) | 0.74 ± 0.12 **** (0.73 ± 0.09) | <0.0001 |
Characteristic | Sucrose | Xylitol | Sorbitol | Erythritol | (ANOVA) p |
---|---|---|---|---|---|
Time | OD540 mean ± SD (median ± QD) | ||||
12 h | 0.12 ± 0.00 (0.12 ± 0.00) | 0.13 ± 0.01 (0.13 ± 0.00) | 0.12 ± 0.00 (0.12 ± 0.00) | 0.10 ± 0.01 **** (0.11 ± 0.01) | <0.0001 |
24 h | 0.13 ± 0.01 (0.13 ± 0.00) | 0.14 ± 0.01 **** (0.14 ± 0.01) | 0.13 ± 0.01 (0.13 ± 0.00) | 0.11 ± 0.01 **** (0.11 ± 0.00) | <0.0001 |
48 h | 0.14 ± 0.00 (0.14 ± 0.00) | 0.15 ± 0.01 **** (0.15 ± 0.01) | 0.13 ± 0.01 ** (0.13 ± 0.00) | 0.11 ± 0.01 **** (0.11 ± 0.01) | <0.0001 |
72 h | 0.15 ± 0.01 (0.15 ± 0.00) | 0.18 ± 0.02 **** (0.17 ± 0.01) | 0.14 ± 0.01 ** (0.14 ± 0.00) | 0.11 ± 0.01 **** (0.11 ± 0.01) | <0.0001 |
Log(CFU/mL) mean ± SD (median ± QD) | |||||
12 h | 1.39 ± 0.14 (1.39 ± 0.07) | 1.49 ± 0.15 ** (1.47 ± 0.06) | 1.31 ± 0.12 (1.31 ± 0.08) | 1.33 ± 0.12 (1.33 ± 0.07) | <0.0001 |
24 h | 1.43 ± 0.09 (1.43 ± 0.05) | 1.64 ± 0.19 **** (1.57 ± 0.07) | 1.36 ± 0.11 (1.35 ± 0.06) | 1.34 ± 0.12 * (1.35 ± 0.07) | <0.0001 |
48 h | 1.61 ± 0.13 (1.64 ± 0.11) | 1.85 ± 0.19 **** (1.85 ± 0.11) | 1.46 ± 0.15 **** (1.44 ± 0.08) | 1.35 ± 0.12 **** (1.36 ± 0.07) | <0.0001 |
72 h | 1.71 ± 0.12 (1.73 ± 0.07) | 2.24 ± 0.29 **** (2.23 ± 0.16) | 1.53 ± 0.15 *** (1.51 ± 0.05) | 1.36 ± 0.13 **** (1.38 ± 0.08) | <0.0001 |
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Staszczyk, M.; Jurczak, A.; Magacz, M.; Kościelniak, D.; Gregorczyk-Maga, I.; Jamka-Kasprzyk, M.; Kępisty, M.; Kołodziej, I.; Kukurba-Setkowicz, M.; Krzyściak, W. Effect of Polyols and Selected Dental Materials on the Ability to Create a Cariogenic Biofilm–On Children Caries-Associated Streptococcus Mutans Isolates. Int. J. Environ. Res. Public Health 2020, 17, 3720. https://doi.org/10.3390/ijerph17103720
Staszczyk M, Jurczak A, Magacz M, Kościelniak D, Gregorczyk-Maga I, Jamka-Kasprzyk M, Kępisty M, Kołodziej I, Kukurba-Setkowicz M, Krzyściak W. Effect of Polyols and Selected Dental Materials on the Ability to Create a Cariogenic Biofilm–On Children Caries-Associated Streptococcus Mutans Isolates. International Journal of Environmental Research and Public Health. 2020; 17(10):3720. https://doi.org/10.3390/ijerph17103720
Chicago/Turabian StyleStaszczyk, Małgorzata, Anna Jurczak, Marcin Magacz, Dorota Kościelniak, Iwona Gregorczyk-Maga, Małgorzata Jamka-Kasprzyk, Magdalena Kępisty, Iwona Kołodziej, Magdalena Kukurba-Setkowicz, and Wirginia Krzyściak. 2020. "Effect of Polyols and Selected Dental Materials on the Ability to Create a Cariogenic Biofilm–On Children Caries-Associated Streptococcus Mutans Isolates" International Journal of Environmental Research and Public Health 17, no. 10: 3720. https://doi.org/10.3390/ijerph17103720