Discovery of Carcinogenic Liver Fluke Metacercariae in Second Intermediate Hosts and Surveillance on Fish-Borne Trematode Metacercariae Infections in Mekong Region of Myanmar
Abstract
:1. Introduction
2. Material and Methods
2.1. Collection of Cyprinoid Fishes and Identification of Fish Species
2.2. Pepsin Digestion Method
2.3. Identification of Metacercariae
2.4. Analysis of Findings
3. Results
3.1. Infection Status and Prevalence of Fish-Borne Trematode Infection in Freshwater Fishes from Tachileik, Mekong Region of Myanmar
3.2. Fish-Borne Trematode Metacercarial Intensity in Cyprinid Fishes from Tachileik, Mekong Region of Myanmar
3.3. Co-infections of Trematode Metacercariae in Fresh Water Cyprinoid Fishes Collected from Tachileik, Mekong Region of Myanmar
3.4. Morphology of Detected Metacercariae of Fish-Borne Trematode Infections in Freshwater Fishes from Tachileik, Mekong Region of Myanmar
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Number | Fish Species | Total Number | Average Length | Average Width |
---|---|---|---|---|
1 | Barbonymus gonionotus | 20 | 9.5 cm ± 0.2236 | 3 cm ± 0.3244 |
2 | Puntioplites falcifer | 30 | 9.5 cm ± 0.2170 | 3 cm ± 0.2110 |
3 | Systomus rubripinnis | 28 | 9 cm ± 0.3502 | 3 cm ± 0.2377 |
4 | Labiobarbus siamensis | 60 | 10 cm ± 0.219 | 3.3 cm ± 0.3188 |
5 | Henicorhynchus siamensis | 50 | 12.5 cm ± 0.2175 | 3.5 cm ± 0.3023 |
6 | Mystacoleucus marginatus | 100 | 9.5 cm ± 0.2564 | 3.3 cm ± 0.3128 |
7 | Rasbora argyrotaenia | 20 | 10.5 cm ± 0.2236 | 3.5 cm ± 0.3244 |
8 | Systomus orphoides | 10 | 12.5 cm ± 0.9368 | 3.5 cm ± 0.3496 |
9 | Puntius brevis | 100 | 10.5 cm ± 0.2422 | 3.5 cm ± 0.2983 |
10 | Trichogaster trichopterus | 20 | 11.5 cm ± 0.2236 | 3.5 cm ± 0.2511 |
11 | Cyclocheilichthys repasson | 151 | 9.5 cm ± 0.2230 | 3.5 cm ± 0.2804 |
12 | Anabas testudineus | 100 | 9 cm ± 0.2240 | 3 cm ± 0.2876 |
total | 689 |
No | Fish Species | No. of Fish Examined | No. (%) of Fish Infected with FBT | No. (%) of Fish Infected with Ov | No. (%) of Fish Infected with Ht | No. (%) of Fish Infected with Hp | No. (%) of Fish Infected with Hap |
---|---|---|---|---|---|---|---|
1 | Barbonymus gonionotus | 20 | 13 (65) | _ | _ | 13 (65) | _ |
2 | Puntioplites falcifer | 30 | 16 (53.33) | _ | _ | 16 (53.33) | _ |
3 | Systomus rubripinnis | 28 | 17 (60.71) | _ | _ | 17 (60.71) | _ |
4 | Labiobarbus siamensis | 60 | 38 (63.33) | _ | 12 (20) | _ | 38 (63.33) |
5 | Henicorhynchus siamensis | 50 | 28 (56) | _ | _ | _ | 28 (56) |
6 | Mystacoleucus marginatus | 100 | 68 (68) | _ | 38 (38) | 14 (14) | 68 (68) |
7 | Rasbora argyrotaenia | 20 | 14 (70) | _ | 9 (45) | _ | 14 (70) |
8 | Systomus orphoides | 10 | 0 (0) | _ | _ | _ | _ |
9 | Puntius brevis | 100 | 0 (0) | _ | _ | _ | _ |
10 | Trichogaster trichopterus | 20 | 0 (0) | _ | _ | _ | _ |
11 | Cyclocheilichthys repasson | 151 | 125 (82.78) | 4 (2.64) | 35 (23.17) | 12 (7.94) | 125 (82.78) |
12 | Anabas testudineus | 100 | 0 (0) | _ | _ | _ | _ |
Total | 689 | 319 (46.29) |
No | Fish Species | No. of Fish Examined | No. (%) of Fish Infected Ov | Total Metacercariae Detected | Range (Min–Max) | Intensity |
---|---|---|---|---|---|---|
1 | Cyclocheilichthys repasson | 151 | 4 (2.64) | 11 | (2–3) | 2.75 |
No | Fish Species | No. of Fish Examined | No. (%) of Fish Infected Ht | Total Metacercariae Detected | Range (Min–Max) | Intensity |
---|---|---|---|---|---|---|
1 | Labiobarbus siamensis | 60 | 12 (20) | 102 | (3–12) | 8.50 |
2 | Mystacoleucus marginatus | 100 | 38 (38) | 120 | (2–10) | 3.15 |
3 | Rasbora argyrotaenia | 20 | 9 (45) | 10 | (1–2) | 1.11 |
4 | Cyclocheilichthys repasson | 151 | 35 (23.17) | 121 | (1–9) | 3.45 |
Total | 331 | 94 (28.39) | 353 | (1–12) | 3.75 |
No | Fish Species | No. of Fish Examined | No. (%) of Fish Infected Hp | Total Metacercariae Detected | Range (Min–Max) | Intensity |
---|---|---|---|---|---|---|
1 | Barbonymus gonionotus | 20 | 13 (65) | 24 | (1–4) | 1.84 |
2 | Puntioplites falcifer | 30 | 16 (53.33) | 16 | (1) | 1 |
3 | Systomus rubripinnis | 28 | 17 (60.71) | 59 | (1–7) | 3.47 |
4 | Mystacoleucus marginatus | 100 | 14 (14) | 67 | (1–6) | 4.78 |
5 | Cyclocheilichthys repasson | 151 | 12 (7.94) | 130 | (1–21) | 10.83 |
Total | 329 | 72 (21.88) | 296 | (1–21) | 4.11 |
No | Fish Species | No. of Fish Examined | No. (%) of Fish Infected Hap | Total Metacercariae Detected | Range (Min–Max) | Intensity |
---|---|---|---|---|---|---|
1 | Labiobarbus siamensis | 60 | 38 (63.33) | 102 | (1–7) | 2.68 |
2 | Henicorhynchus siamensis | 50 | 28 (56) | 43 | (1–3) | 1.53 |
3 | Mystacoleucus marginatus | 100 | 68 (68) | 120 | (1–5) | 1.76 |
4 | Rasbora argyrotaenia | 20 | 14 (70) | 16 | (1–2) | 1.14 |
5 | Cyclocheilichthys repasson | 151 | 125 (82.78) | 695 | (1–21) | 5.56 |
Total | 381 | 273 (71.65) | 976 | (1–21) | 3.57 |
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Phyo Myint, E.E.; Sereemaspun, A.; Rocklöv, J.; Nithikathkul, C. Discovery of Carcinogenic Liver Fluke Metacercariae in Second Intermediate Hosts and Surveillance on Fish-Borne Trematode Metacercariae Infections in Mekong Region of Myanmar. Int. J. Environ. Res. Public Health 2020, 17, 4108. https://doi.org/10.3390/ijerph17114108
Phyo Myint EE, Sereemaspun A, Rocklöv J, Nithikathkul C. Discovery of Carcinogenic Liver Fluke Metacercariae in Second Intermediate Hosts and Surveillance on Fish-Borne Trematode Metacercariae Infections in Mekong Region of Myanmar. International Journal of Environmental Research and Public Health. 2020; 17(11):4108. https://doi.org/10.3390/ijerph17114108
Chicago/Turabian StylePhyo Myint, Ei Ei, Amornpun Sereemaspun, Joacim Rocklöv, and Choosak Nithikathkul. 2020. "Discovery of Carcinogenic Liver Fluke Metacercariae in Second Intermediate Hosts and Surveillance on Fish-Borne Trematode Metacercariae Infections in Mekong Region of Myanmar" International Journal of Environmental Research and Public Health 17, no. 11: 4108. https://doi.org/10.3390/ijerph17114108