Effect of Hyaluronic Acid Filler Injection on the Interdental Papilla in a Mouse Model of Open Gingival Embrasure
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal
2.2. OGE Model
2.3. Injection of HA Filler into the IDP
2.4. Tissue Preparation
2.5. Immunohistochemistry
2.6. Statistical Analysis
3. Results
3.1. Morphological and Histological Changes in IDP after Wire Attachment
3.2. Morphological and Histological Changes in IDP after PBS or HA Filler Injection
3.3. Immunolocalization of Ki67 and Inflammatory Cytokines after PBS or HA Filler Injection
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Antibody | Marked Cell | Localization Pattern | |||
---|---|---|---|---|---|
PBS Injection | HA Filler Injection | ||||
Day 2 | Day 7 | Day 2 | Day 7 | ||
Ki67 | Cell proliferation/nuclear | Basal cells of epithelium, Connective tissue around the injected material | Basal cells of epithelium | Basal cells of epithelium, Connective tissue around the injected material | Basal cells of epithelium |
IL-1β | Macrophages/fibroblasts | Connective tissue cells | - | Connective tissue cells | - |
IL-6 | Macrophages/fibroblasts | Very few cells in connective tissue | |||
TNF-α | Activated macrophages | Connective tissue cells | - | Connective tissue cells | - |
MPO | Neutrophils | Connective tissue around the injected material | - | Connective tissue around the injected material | - |
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Kim, S.-B.; Cho, J.; Jue, S.-S.; Park, J.H.; Kim, J.-Y. Effect of Hyaluronic Acid Filler Injection on the Interdental Papilla in a Mouse Model of Open Gingival Embrasure. Int. J. Environ. Res. Public Health 2020, 17, 4956. https://doi.org/10.3390/ijerph17144956
Kim S-B, Cho J, Jue S-S, Park JH, Kim J-Y. Effect of Hyaluronic Acid Filler Injection on the Interdental Papilla in a Mouse Model of Open Gingival Embrasure. International Journal of Environmental Research and Public Health. 2020; 17(14):4956. https://doi.org/10.3390/ijerph17144956
Chicago/Turabian StyleKim, Soo-Bin, Jaehun Cho, Seong-Suk Jue, Jae Hyun Park, and Ji- Youn Kim. 2020. "Effect of Hyaluronic Acid Filler Injection on the Interdental Papilla in a Mouse Model of Open Gingival Embrasure" International Journal of Environmental Research and Public Health 17, no. 14: 4956. https://doi.org/10.3390/ijerph17144956
APA StyleKim, S. -B., Cho, J., Jue, S. -S., Park, J. H., & Kim, J. -Y. (2020). Effect of Hyaluronic Acid Filler Injection on the Interdental Papilla in a Mouse Model of Open Gingival Embrasure. International Journal of Environmental Research and Public Health, 17(14), 4956. https://doi.org/10.3390/ijerph17144956