Leptin Upregulates the Expression of β3-Integrin, MMP9, HB-EGF, and IL-1β in Primary Porcine Endometrium Epithelial Cells In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Treatments
2.2. RNA Extraction, Reverse Transcription, and Real-Time PCR
2.3. Protein Extraction and Western Blot Analysis (WB)
2.4. Statistical Analysis
3. Results
3.1. Leptin Induced the Expression of β3-Integrin, MMP9, HB-EGF, and IL-1β in PEECs
3.2. Phosphorylation of JAK, Akt, and Erk Induced by Leptin Could Be Attenuated by AG490, LY294002, and U0126
3.3. JAK2, PI(3)K, and MAPK Signaling Pathways Were Involved in the Leptin Upregulation on β3-Integrin, MMP9, HB-EGF, and IL-1β in PEECs
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Gene No. | Primer Sequence | Length (bp) |
---|---|---|---|
β3-integrin | NM_214002 | F: 5′-CTTCTATTTGGGAGTGAG-3′ | 167 |
R: 5′-AGGTGATGCTGGTCTAA -3′ | |||
MMP9 | NM_001038004 | F: 5′-CACGCATTGGGCTTAGAT-3′ | 130 |
R: 5′-TAGGGCGAGAACCATACA-3′ | |||
HB-EGF | NM_214299 | F:5′-AAAGAAGAAAGGCAAAGGG-3′ | 242 |
R: 5′-GACAGACGGACGACAGCA-3′ | |||
IL-1β | NM_001005149 | F:5′-AAGTGATGGCTAACAATG-3′ | 266 |
R: 5′-TTCTTCAAAGACGGATG-3′ | |||
GAPDH | NM_001206359.1 | F: 5′-GTCCACTGGTGTCTTCACGA-3′ | 145 |
R: 5′-GCTGACGATCTTGAGGGAGT-3′ |
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Wang, H.; Fu, J.; Wang, A. Leptin Upregulates the Expression of β3-Integrin, MMP9, HB-EGF, and IL-1β in Primary Porcine Endometrium Epithelial Cells In Vitro. Int. J. Environ. Res. Public Health 2020, 17, 6508. https://doi.org/10.3390/ijerph17186508
Wang H, Fu J, Wang A. Leptin Upregulates the Expression of β3-Integrin, MMP9, HB-EGF, and IL-1β in Primary Porcine Endometrium Epithelial Cells In Vitro. International Journal of Environmental Research and Public Health. 2020; 17(18):6508. https://doi.org/10.3390/ijerph17186508
Chicago/Turabian StyleWang, Hongfang, Jinlian Fu, and Aiguo Wang. 2020. "Leptin Upregulates the Expression of β3-Integrin, MMP9, HB-EGF, and IL-1β in Primary Porcine Endometrium Epithelial Cells In Vitro" International Journal of Environmental Research and Public Health 17, no. 18: 6508. https://doi.org/10.3390/ijerph17186508