Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling Sites and Time
2.2. DNA Extraction, Conventional PCR and qPCR
2.2.1. DNA Extraction
2.2.2. Conventional PCR
2.2.3. qPCR
2.3. Statistical Analysis
3. Results
3.1. ARGs Occurrence in Water Bodies
3.2. RT-qPCR Analysis of ARGs
3.3. Redundancy Analysis of the Correlations
4. Discussion
4.1. Detection of the ARGs
4.2. Absolute Abundance of ARGs
4.3. Time and Spatial Distribution of ARGs
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Su, S.; Li, C.; Yang, J.; Xu, Q.; Qiu, Z.; Xue, B.; Wang, S.; Zhao, C.; Xiao, Z.; Wang, J.; et al. Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea. Int. J. Environ. Res. Public Health 2020, 17, 552. https://doi.org/10.3390/ijerph17020552
Su S, Li C, Yang J, Xu Q, Qiu Z, Xue B, Wang S, Zhao C, Xiao Z, Wang J, et al. Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea. International Journal of Environmental Research and Public Health. 2020; 17(2):552. https://doi.org/10.3390/ijerph17020552
Chicago/Turabian StyleSu, Sicong, Chenyu Li, Jiping Yang, Qunying Xu, Zhigang Qiu, Bin Xue, Shang Wang, Chen Zhao, Zhonghai Xiao, Jingfeng Wang, and et al. 2020. "Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea" International Journal of Environmental Research and Public Health 17, no. 2: 552. https://doi.org/10.3390/ijerph17020552
APA StyleSu, S., Li, C., Yang, J., Xu, Q., Qiu, Z., Xue, B., Wang, S., Zhao, C., Xiao, Z., Wang, J., & Shen, Z. (2020). Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea. International Journal of Environmental Research and Public Health, 17(2), 552. https://doi.org/10.3390/ijerph17020552