Two-Photon Laser Ablation and In Vivo Wide-Field Imaging of Inferior Olive Neurons Revealed the Recovery of Olivocerebellar Circuits in Zebrafish
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fish and Transgenic Lines
2.2. Two-Photon Laser Ablation
2.3. Confocal Microscopy Imaging
2.4. Cell Counting
2.5. Whole-Mount Immunostaining
3. Results
3.1. Distribution of Inferior Olive Neurons in Zebrafish
3.2. Two-Photon Laser Ablation of IO Neurons
3.3. Recovery of the Olivocerebellar Circuit after IO Ablation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hiyoshi, K.; Saito, K.; Fukuda, N.; Matsuzaki, T.; Yoshikawa, H.Y.; Tsuda, S. Two-Photon Laser Ablation and In Vivo Wide-Field Imaging of Inferior Olive Neurons Revealed the Recovery of Olivocerebellar Circuits in Zebrafish. Int. J. Environ. Res. Public Health 2021, 18, 8357. https://doi.org/10.3390/ijerph18168357
Hiyoshi K, Saito K, Fukuda N, Matsuzaki T, Yoshikawa HY, Tsuda S. Two-Photon Laser Ablation and In Vivo Wide-Field Imaging of Inferior Olive Neurons Revealed the Recovery of Olivocerebellar Circuits in Zebrafish. International Journal of Environmental Research and Public Health. 2021; 18(16):8357. https://doi.org/10.3390/ijerph18168357
Chicago/Turabian StyleHiyoshi, Kanae, Kaito Saito, Narumi Fukuda, Takahisa Matsuzaki, Hiroshi Y. Yoshikawa, and Sachiko Tsuda. 2021. "Two-Photon Laser Ablation and In Vivo Wide-Field Imaging of Inferior Olive Neurons Revealed the Recovery of Olivocerebellar Circuits in Zebrafish" International Journal of Environmental Research and Public Health 18, no. 16: 8357. https://doi.org/10.3390/ijerph18168357