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Review

Cardiovascular Disease and Chronic Endodontic Infection. Is There an Association? A Systematic Review and Meta-Analysis

by
Despina Koletsi
1,*,
Anna Iliadi
2,
Giorgos N. Tzanetakis
3,
Manolis Vavuranakis
4 and
Theodore Eliades
1
1
Clinic of Orthodontics and Pediatric Dentistry, Center of Dental Medicine, University of Zurich, CH-8032 Zurich, Switzerland
2
Department of Dental Biomaterials, School of Dentistry, National and Kapodistrian University of Athens, 10679 Athens, Greece
3
Department of Endodontics, School of Dentistry, National and Kapodistrian University of Athens, 10679 Athens, Greece
4
First Cardiology Department, National and Kapodistrian University of Athens, Hippocration Hospital, 10679 Athens, Greece
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2021, 18(17), 9111; https://doi.org/10.3390/ijerph18179111
Submission received: 6 July 2021 / Revised: 26 August 2021 / Accepted: 27 August 2021 / Published: 29 August 2021
(This article belongs to the Section Oral Health)
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Abstract

:
The aim of the present study was to systematically assess existing evidence on the possible association between chronic endodontic infections and cardiovascular disease (CVD). An electronic database search was implemented until 2 October 2020. The main outcome was risk of CVD diagnosis. Risk of bias was assessed through the ROBINS-I tool, while random effects meta-analyses were conducted. The quality of the evidence was assessed with the Grading of Recommendations Assessment, Development, and Evaluation. Twenty-one studies were eligible for inclusion, while 10 were included in the quantitative synthesis. Risk for CVD diagnosis in patients with chronic endodontic infection was 1.38 times those without infection (RR = 1.38; 95% CIs: 1.06, 1.80; p = 0.008). Risk of bias ranged from moderate to serious, while the quality of the evidence was graded as very low. Indications for an identified association between chronic endodontic infection and CVDs do exist; however, they are not grounded on high-quality evidence at present. Further research for an establishment of an association based on temporal sequence of the two entities and on unbiased well-conducted cohort studies would be highly valued.

1. Introduction

The global burden of cardiovascular diseases (CVDs), comprising ischemia, ischemic episode, coronary heart disease, stroke, arterial/vascular disease, heart defects, and others, has been widely recognized as of utmost severity and importance for public and community health, and they are also currently identified as the most common non-communicable diseases worldwide. The latest scheming for CVDs impact has revealed a proximal calculation of 330 million years of life lost worldwide, with a corresponding number of 17.8 million deaths [1]. The figures may evidently appear daunting; however, a large number of potentially prognostic factors have been identified and are continuously under close investigation for documentation of association effects with CVDs-related mortality and prediction risk model estimators; efforts to this direction are unceasingly intense, due to the substantial impact of CVDs on everyday life health state and related morbidity [2].
The most common risk factors for CVDs include smoking status, history of diabetes mellitus, blood cholesterol levels, and increased systolic blood pressure [3]. Risk models and confounder standardized calibrated procedures are currently followed in an attempt to provide the most rigorous evidence on the combined fatal and non-fatal events and related risk classification [4]. Interestingly, there has been ongoing research since the start of the millennium, with respect to the identification of potential associations between oral conditions, more specifically inflammation of periodontal tissues and CVDs [5]. This cross-linkage originates form the inherent microbial burden associated with the periodontal disease and its potential for systemic implications [6,7]. Periodontal disease or marginal periodontitis is a well-established inflammatory process of the supporting tooth structures, comprising the periodontal ligament, tooth cementum, and alveolar bone socket, while it affects almost ≈15% of the population in varying degrees. The clinical and radiographic picture of marginal periodontitis is framed under tooth flaring and mobility as well as reduction in the marginal bone support of the teeth in variable levels [5]. Evidence exists on several epidemiologic indicators with regard to periodontitis and CVDs. There is growing evidence for a positive association between marginal periodontitis and coronary heart disease, as confirmed by the latest consensus report of periodontal research [8]. Underlying mechanisms of such an association suggest that oral bacterial species may enter circulation and induce bacteremia [9,10], while the presence of oral bacteria has also been confirmed in atheromatic lesions [11].
On the same grounds, there is growing concern that alternative-origin oral cavity-related chronic inflammatory conditions might be an additional triggering factor for the advent of CVDs. One of the most concerning clinical entities reported is root canal infection and subsequent inflammation of periapical tissues after pulp necrosis described under the term “apical periodontitis”. Apical periodontitis (AP) is an inflammatory disease caused by the establishment of microbial infection within the root canal system of the tooth, resulting in inflammatory periapical tissue response and apically framed bone destruction, as is also evident as a radiolucency in periapical radiographs [12,13]. In turn, this may be related to elevated systemic concentrations of inflammatory mediators or reactive peripheral blood cells, impacting on general cardiovascular health status.
To date, a number of epidemiologic studies have attempted to investigate individual associations between chronic endodontic infections and CVDs; however, strong correlations appear hard to establish due to the inherent risk of bias and threats to the internal validity identified in those studies [14,15,16]. A recent umbrella review [17] has identified four systematic reviews on the topic, which included published studies until four years ago, while a meta-analysis of four studies was performed in only one of those; however, since then, a number of potentially eligible for investigation studies have emerged in the literature [16,18,19], while most importantly, the existing systematic reviews have been graded as critically low to moderate quality, in terms of methodological/reporting inconsistencies followed during the review process [17].
Evidently so, an updated attempt to appraise and synthesize the most recent epidemiologic data in the field is considered timely in order to provide an unequivocal picture of endodontic infections and their association with CVDs, following rigorous and transparent methodology, while also allowing for further insights. In addition, evidence of association, undeniably under the spectrum of causality effects and methodologic limitations of the included studies, might prove a useful guide for future prognostic risk factors to be considered broadly, when estimating individual or integrated effects on CVDs. Therefore, the aim of the present study was to systematically collect and appraise the existing contemporary evidence on the association between chronic endodontic infections and CVDs.

2. Materials and Methods

2.1. Protocol, Registration, Reporting

The protocol of this study was registered with the Open Science Framework (osf.io/jafgd) [20]. Reporting was conducted in line with the MOOSE guidelines, in view of the anticipated observational design of the potentially eligible for inclusion studies [21,22].

2.2. Eligibility Criteria

Eligibility criteria for study selection were structured as follows:
  • Study design: any type of observational study, irrespective of the design, i.e., retrospective cohort, prospective cohort, case-control, cross-sectional.
  • Participants: participants of any age and gender.
  • Condition of interest/exposure: any type of periapical or periradicular lesion indicating infection of the root canal system and inflammation of periapical tissues (also reported as “apical periodontitis”), as confirmed clinically or radiographically/also assessed through medical file records.
  • Outcome: any outcome related to CVDs, incidence or prevalence of the disease, including but not confined to coronary heart disease, stroke, myocardial infraction, arterial/vascular disease.
  • Self-reporting of the disease or the exposure were also considered, but specifically indicated as such, while for cross-sectional study design, the terms exposure/outcome were used interchangeably.
  • Exclusion criteria: case studies, case reports with less than 10 participants.

2.3. Search Strategy and Study Selection

Initial electronic searching was conducted within both published and unpublished literature, with no chronologic restriction, or other limits applied. The following Electronic Databases were searched as of May 2020 and updated 2 October 2020: MEDLINE via PubMed, Scopus, Cochrane Central Register of Controlled Trials (CENTRAL), Cochrane Database of Systematic Reviews (CDSR). In addition, gray literature was searched through dissertation sources and in Open Grey, the ClinicalTrials.gov (www.clinicaltrials.gov), and the National Research Register (www.controlled-trials.com). Hand searching of the articles eligible for inclusion was also employed. Keywords included “cardiovascular disease”, “coronary heart disease”, “endodontic infection”, “chronic endodontic infection”, and “apical periodontitis”. The search strategy for PubMed is presented in Appendix A.

2.4. Data Collection

Data extraction was employed in pre-piloted standardized forms by two independently working reviewers (DK, AI) not blinded to study origin or author identity. Specifically, information entries were related to study identity, study design, sample size, condition/exposure, outcomes, and further study-specific information.

2.5. Risk of Bias in Individual Studies

Risk of bias assessment was performed independently by one author (DK), and all recordings were confirmed by a second (AI). Any disagreements were settled after consultation with a third author (TE). The ROBINS-I tool was used to assess internal validity of the included studies, in view of the anticipated observational design of the latter [23].

2.6. Summary Measures and Data Synthesis

Prior to any decision to quantitatively pool together data from individual studies, clinical heterogeneity was examined in terms of individual study settings and conditions, population characteristics, eligibility criteria, or analyses. Statistical heterogeneity was examined, first visually, through inspection of the confidence bounds within the forest plots, as well as statistically, as indicated by a p-value below the level of 10% for the test (p < 0.10). An I2 test for homogeneity was also undertaken to quantify the extent of heterogeneity.
Random effects meta-analyses were conducted as they are considered more appropriate to incorporate individual study findings. In view of the anticipated dichotomous nature of the expected outcomes, pooled treatment effects were calculated through risk ratios (RRs) with associated 95% confidence intervals (95% CIs) and prediction intervals (95% PIs). Study authors were contacted when additional data and information were required that could not be retrieved by the publication record.

2.7. Risk of Bias across Studies

Publication bias was explored through standard funnel plots and Egger’s regression test [24].

2.8. Additional Analyses

Sensitivity analyses were considered, if applicable, to explore and isolate the effect of studies with serious/critical risk of bias on the overall effect, if studies of both serious/critical or lower risk of bias were included. In addition, the effect of studies including participants with well-known underlying diseases (i.e., diabetes) in the final sample, or smoking, was removed by excluding the studies from separate sensitivity analyses. If a wide age range was to be identified across included studies, subgroup analyses were also considered as appropriate. Recording of outcomes based on patient self-reporting was also considered separately through sensitivity analysis. Lastly, an all-bias inclusive sensitivity analysis was performed by excluding all aforementioned potential sources of uncontrolled confounding or bias.

2.9. Assessment of the Quality of the Evidence

The Grading of Recommendations Assessment, Development and Evaluation (GRADE) was implemented to assess the overall quality of the evidence as formulated by the conditions/exposures and outcomes for evaluation. According to GRADE, the overall body of evidence is rated as high, moderate, low, and very low. The ratings, with regard to the likelihood for a change in our confidence in the estimated effect, range from very unlikely to very likely, to be modified [25,26]. Assessment of the body of evidence primarily involves assessment of study design. Assessment is made on the following domains: risk of bias, inconsistency, indirectness, imprecision, and publication bias. For the first four domains, the quality of evidence may be downgraded on the basis of either ‘serious’ or ‘very serious’ risks (1 or 2 levels, respectively); publication bias may either be suspected or undetected. For non-randomized/observational designs specifically, which theoretically start from a ‘low’ level of evidence, the perspectives for upgrade are as follows: a large or very large effect, a plausible residual confounding that may alter the effect, or a dose–response gradient. The level of evidence may be upgraded by 1 or 2 levels (large effect), or 1 level (plausible confounding, dose–response gradient).

3. Results

3.1. Search Details

The study selection process and the ultimate number of included articles in qualitative and quantitative synthesis is presented in Figure 1. From an initial hit of 1393 results, 21 articles passed through a full-text screening process to the qualitative synthesis [14,15,16,18,19,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42]. Of those, 10 papers contributed to meta-analyses and/or additional sensitivity analysis [14,15,18,27,28,31,36,37,40,41]. Reasons for exclusion of articles are outlined in Figure 1.

3.2. Study Design and Characteristics

Detailed characteristics of included studies are presented in Table 1. Of the 21 studies, six cohort studies were identified—four retrospective and two prospective—while the majority (n = 14) were of cross-sectional design. Only one case-control study was detected. Sample sizes for cohort studies ranged from 278 [31] to 283,590 [35]. The respective numbers for cross-sectional studies were 55 [30] to 666,768 [18] participants, while the sole case-control study reported 100 patients [36]. Three studies involved only male patients [14,16,33], one study only female patients [29], while the rest involved both sexes. The reported age of participants was more than a mean of 45 years for the two-thirds of the included studies (n = 14), while seven studies included younger age ranges in conjunction with older or in isolation [14,16,18,28,30,32,35].
Cardiovascular disease entities and cases identified as outcomes in the present study involved mostly hard measures of prevalence/incidence of CVDs in general, coronary heart disease, including coronary artery disease, coronary artery atherosclerosis/atherosclerotic burden, myocardial infraction, or unstable angina. Only a small proportion of studies involved other proxy measures for CVD outcomes, such as C-reactive protein, flow-mediated dilation, or hypertension [16,30,38,39]. Chronic endodontic infection was vastly confirmed through radiographic examination in all but one study [19]. The diagnosis of the endodontic condition described by most studies pertained to the reporting of diagnosis of “apical periodontitis”, “lesion of endodontic origin”, “endodontic pathology”, and “periapical destruction”. In a small proportion of studies, the description of the condition pertained to endodontic therapy, root canal treatment, and unfinished root canal treatment [19,33,35]. The latter do not constitute robust and definite measures of infections of endodontic origin, or even proxies for this, within the frame of the designs under investigation, and as such, they were not considered for the quantitative synthesis (Table 1).

3.3. Risk of Bias within Studies

Overall, risk of bias was rated as moderate in 11 of the included studies, while it was rated as serious in the remaining 10. The most severely impacted domains were confounding by potentially undisclosed, undetermined, or non-controlled risk factors, classification of the conditions/exposures, and measurement of the outcomes. Both latter domains pertain to a potential lack of blinding of the investigators, who were involved in the assessment of either the exposure or the outcome. In addition, although the specific domain of “selection of the reported result” was uniformly classified as “low risk of bias”, it should be noted that none of the included studies described pre-registration of a related protocol for the study; however, correspondence between the described methodology in the article and reported analyses and results was considered acceptable (Table 2; Table A1).

3.4. Effects of Interventions, Meta-Analyses, Additional Analyses

As previously noted, 10 studies contributed to meta-analysis or additional analyses [14,15,18,27,28,31,36,37,40,41]. Studies reporting on any hard cardiovascular outcomes as described above, excluding proxy measures, and definite documentation of lesions of endodontic origin/apical periodontitis were considered eligible for quantitative synthesis. According to the overall estimate for the perceived association between the two entities (i.e., lesion of endodontic origin and CVDs), patients with a diagnosis of chronic endodontic infection/apical periodontitis had 38% higher risk of being diagnosed with a CVD (10 studies: RR = 1.38; 95%CI: 1.06, 1.80; p = 0.008); however, prediction intervals (95%PIs) included the null and were recorded as 0.55 to 3.49, illustrating the variability of the true effect in different conditions, studies, or settings. Furthermore, although the effect was not obvious solely for the two cohort studies eligible for inclusion in the analysis (two studies, subgroup analysis: RR = 1.08; 95%CIs: 0.64, 1.83; p = 0.78), the results of the meta-regression analysis, with Knapp–Hartung modification, built to identify any effect of the study design on the summary effect, did not reveal a significant association (p = 0.61). In essence, the scarcity of studies of this design included in the meta-analyses might have blurred the real effect, providing a decreased power to identify the association (Table 3, Figure 2).
Sensitivity analysis considered age range, baseline similarity of important confounders (matching across groups on diabetes/smoking), and self-reporting of outcomes assessed, and the results of these analyses are outlined in Table 3. Evidently, the effect pertained to all subsequent analyses: for example, when only patients more than a mean of 45 years of age were included, the associated risk for CVDs elevated to 46% higher for patients with chronic endodontic infection/apical periodontitis (RR = 1.46; 95%CIs: 1.19, 1.79; p < 0.001), as opposed to those without. Likewise, when only studies with moderate risk of bias were included [15,31,36,37], the effect appeared stronger, with RR = 1.86 (95%CIs: 1.37, 2.52; p < 0.001) and apparently lower heterogeneity across the subsample (I2 = 50.7; p = 0.11).
Publication bias was detected. A contour-enhanced funnel plot revealed a relative absence of published studies with null effect and non-significant results. Studies were missing from the inner central and lower left part of the plot (Figure 3), while Egger’s test for small-study effects was apparently significant (p = 0.003).

3.5. Quality of the Evidence

The quality of the existing evidence for the association between chronic endodontic infection/apical periodontitis and CVDs was very low. The design of the included studies along with decisions to downgrade the quality of the evidence due to between-study heterogeneity and publication bias issues contributed to this rating (Table A2). Apparently, this means that we are uncertain about the effect estimate, and further research is very likely to change our confidence with regard to this effect.

4. Discussion

4.1. Findings in Context

A rough estimator of the results of the present review has indicated great uncertainty with regard to the perceived association between endodontic infections and CVDs. In essence, there appears to be a substantial amount of individual attempts to elucidate the causal mechanisms of these associations; however, most research endeavors have failed to prove successful, as study design and potential confounders or independently but simultaneously co-acting factors continue to impose a non-negligible amount of obscurity on causality pathways. Thus, the identification of an elevated risk for cardiovascular-related diseases, as implied by the present synthesis of available evidence, should be placed into the appropriate context, conditional on the retrieved weaknesses of the included original study designs.
Effectively, efforts have been ongoing for the last 15 years, and with an increasing intensity in the past 5 to 6 years, to shed light on regional, endodontic infections and their impact on general health either in the short or long term. This observation might have been partially endorsed by the most recent estimations for an anticipated increase in the prevalence of CVDs within the years to come of approximately 10% [43]; moreover, it is also endorsed by the WHO estimations for approximately 24 million patients losing their lives to CVDs by the year 2030 [3]. Interestingly, lately, CVDs have been associated with the path and prognosis of COVID-19 patients [44], with autopsy material confirming a cytokine-mediated exacerbation and related inflammation in CVD patients also positive for COVID-19, thus pinpointing the central role of a call for further endorsements on the investigation and research against any potential risk factor for CVDs, as seasonal epidemics or pandemics may potentially pose a greater burden for the morbidity of the disease at present or in the near future.
Cardiovascular entities entail a large group of non-communicable diseases framed under coronary heart disease, stroke, atherosclerosis, and myocardial infraction and affecting the human heart and blood vessels [45]. A key and commonly shared element is the presence of vascular events and systemic implications for the individual. To this end, it has been suggested that various factors might be implicated in the pathogenic mechanisms of such a condition, including underlying grounds of diabetes mellitus and rheumatoid arthritis [46]. An important consideration when evaluating the onset of CVDs is the close investigation of other entities that may run ahead and parallel with the CVDs and are effectively served by inflammation, tissue damage, and macrovascular circulation-related issues [47,48]. The results include the development of atheromatosis or atherosclerosis, carotid plaque, and luminal narrowing of arteries [49], with most severe endpoints being stroke, myocardial infraction, and mortality. A non-negligible amount of studies included in the present review involved participants with underlying diabetes disease, thus potentially serving as a positive confounder for the investigated association; however, sensitivity analysis, after the elimination of those studies, indicated the persistence of the recorded effect and its direction.

4.2. Prior Research

In the same line and following the scheme for identifying common pathogenic mechanisms and/or triggering factors for the development of a potentially disrupting environment for the heart and vessels, researchers in dental medicine have been studying the effect of chronic infections of endodontic origin (i.e., pulp necrosis/root canal infection) on CVDs [14,15,18].
Acute inflammation of dental pulp, due to caries or traumatic injury, may result in pulp necrosis and subsequent tissue infection, bearing an increased dynamic to induce the formation of apical periodontitis in proximity to the infected internal tooth structures [50]. A failed root canal treatment may also serve as a proxy for the initiation of apical periodontitis. Such lesions of endodontic origin appear as a result of bacterial and microbial pathogen transmission from the infected pulp space to the periapical region of the tooth; in essence, bone destruction in the periapical region is additionally described as the endpoint of a surplus immune system reaction to inflammation [51]. A range of inflammatory biomarkers have been recognized as playing a central role in the pathogenesis and physiology of lesions of endodontic origin. A very recent review has outlined the basis of inflammatory response to apical periodontitis and has elucidated the biologic mechanisms involved. Integral compounds of this are neutrophils at the initial and most acute phase followed by the recruitment and infiltration of mast cells and macrophages as a second-line response, which are allied to the chronicity of infection. What is more, prostaglandins, cytokines, and chemokines produced are associated with the recruitment of leukocytes [51,52,53].
Evidently, potential associations with the advent of CVDs might be presumed and considered biologically plausible, since bacteria that originated from infected tissues coupled with by-products of the inflammatory process and the formation of granulomas might interact and enter systemic circulation with an impact on major blood vessels. Interestingly, efforts to ameliorate the progression of chronic apical periodontitis have been reported to present a positive effect on endothelial dysfunction biomarkers, as demonstrated by a successful root canal treatment of the affected teeth [54]. A similar picture has long been elucidated for periodontal disease and cardiovascular outcomes [8], while lately, research has been targeted towards the discovery of shared genetic background and risk loci of the two entities [55].

4.3. Clinical Implications

Despite weak evidence for an association between chronic endodontic infection/apical periodontitis and CVDs, additional to the limitations of the included studies, clinical cardiologists should consider chronic endodontic infections as potential proxies for adverse effects in patients with CVDs. A similar association has already been acknowledged for other oral pathologic conditions such as periodontal disease [8]. To this end, clinical and radiographic evaluation of patients’ oral condition, teeth, and supporting tissues is paramount. Dentists and oral health practitioners may play a central role in prevention strategies related to prognostic factors for CVDs. Moreover, the radiographic examination of patients with CVDs through standard panoramic radiographs may aid to accurately detect existing periapical inflammatory lesions and advise patients to proceed to further clinical examination to assess whether these lesions are active or not, thus minimizing any potential risk for systemic effects of inflammatory mediators and bacteremia.

4.4. Strengths and Limitations

Compared to prior reports in the field [56,57,58,59], this is the first systematic attempt that comprehensively and transparently assessed all available scientific evidence, with a quantitative synthesis of 10 studies, offering increased precision regarding the estimated effect and association, while focusing on placing available evidence from potentially variant sources under the appropriate context in terms of research quality, quality of the evidence, and/or reporting quality. The presence of significant shortcomings of prior studies in the field, such as heterogeneity issues related to patients’ characteristics and studies’ settings, or confounding effects were also addressed with appropriate sensitivity analyses, which added to the methodological robustness of the present report. In addition, only hard cardiovascular outcomes were considered in the meta-analyses, excluding biomarkers or proxy measures that might have suffered from uncertainty in the establishment of a certain diagnosis. After pre-registration to an openly available framework [20], a systematic search was conducted in published and unpublished literature across seven databases, while this is to our knowledge the first formal attempt to statistically and visually explore the presence of publication bias in the field; this held true, based on the estimated effects of the retrieved studies alongside their variances. Furthermore, the quality of the existing evidence and its implications were assessed and explored with the GRADE framework, rendering initiatives for further well-conducted and reported research highly anticipated.
The study was not free of limitations, though. First, the internal validity of the included studies was in broad terms suboptimal, thus impacting on the interpretation of the results of the conducted syntheses. Second, the majority of studies were of cross-sectional design, with additional bias-related issues--for example, self-reporting of endodontic lesions—or inclusion of other co-morbidities in the samples achieved and non-confirmed temporal sequence of the investigated conditions. In addition, the possibility of the long-term silent existence of a necrotic pulp only, without periradicular spread of the infection or inflammation and thus apparently forming a reduced probability of detection through radiographic examinations, might be an additional factor related to underestimation of the perceived association; however, this is always conditional on the study design.

5. Conclusions

In consideration of all identified and discussed caveats, an association between chronic endodontic infection and CVDs may not be disregarded, although being of limited evidence quality at present, because the biologic background of such a relation is plausible. Targeting and identifying any single risk factor for a worldwide health-impacting disease seems imperative, which must be based on the most high-quality evidence. Certainly, further research with additional focus on the temporal circumstances and establishment of the association under study, while also on vastly well-conducted and reported studies, should be greatly valued.

Author Contributions

Conceptualization, D.K., G.N.T. and T.E.; methodology, D.K., A.I. and T.E.; validation, D.K., A.I., G.N.T., M.V. and T.E.; formal analysis, D.K.; investigation, D.K. and A.I.; resources, D.K., A.I., G.N.T., M.V. and T.E.; data curation, D.K. and A.I.; writing—original draft preparation, D.K.; writing—review and editing, D.K., A.I., G.N.T., M.V. and T.E.; visualization, D.K., A.I., G.N.T., M.V. and T.E.; supervision, D.K. and T.E.; project administration, D.K. and T.E.; All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are available by the corresponding author upon reasonable request.

Conflicts of Interest

The authors declare no conflict of interest.

Appendix A

MEDLINE via Pubmed. Date of search: 2.10.2020. Limits: none. Hits: 863.
(apical periodontitis) OR (chronic apical periodontitis) OR (apic * periodont *) OR (tooth periapical lesion) OR (teeth periapical lesion) OR (tooth apical lesion) OR (teeth apical lesion) OR (chronic tooth inflammation) OR (chronic pulp teeth inflammation) OR (chronic teeth inflammation) OR (radiolucent periapical teeth lesion) OR (radiolucent periapical tooth lesion) OR (tooth root canal treatment) OR (endodontic treatment) OR (root canal tooth therapy) OR (endodontic infection) OR (tooth pulp* infection) OR (chronic dental infection)) AND (coronary disease) OR (coronary heart disease) OR (cardiovascular disease) OR (hypertension) OR (atherosclerosis) OR (CVD) OR (stroke) OR (myocardial infraction)) NOT (diabetes) OR (metabolic)).
Table A1. Detailed assessment of ROBINS-I tool for the included studies (supplement to Table 2).
Table
(A)
(A)
DomainReferenceAn et al., 2016Caplan
et al., 2006		Chauhan et al., 2019Costa et al., 2014Cowan
et al., 2020		De Oliveira et al., 2017Freidlander
et al., 2010		Frisk
et al., 2003		Garrido
et al., 2019
1. Confounding1.1PYPNPYPYPNPYPYPYPY
1.2NANANANANANANANANA
1.3NANANANANANANANANA
1.4PYNAPNPYNAPNPNPYPY
1.5PYNANAPYNANANAPYPY
1.6NANANINANANININANA
1.7NANAPNNANAPNPNNANA
1.8NANANANANANANANANA
JudgementModerateLowSeriousModerateLowSeriousSeriousModerateModerate
2. Selection of participants into the study2.1NNPNPNPNPNPNPNPN
2.2NANANANANANANANANA
2.3NANANANANANANANANA
2.4NININININININININI
2.5NANANANANANANANANA
JudgementNININININININININI
3. Classification of interventions3.1PYYPYPYYPYYPYPY
3.2PYYPYPYYPYYPYPY
3.3NNNPYNPYNPYPY
JudgementLowLowLowSeriousLowSeriousLowSeriousSerious
4. Deviations from intended interventions4.1PNPNPNPNPNPNPNPNPN
4.2NANANANANANANANANA
4.3NANANANANANANANANA
4.4NANANANANANANANANA
4.5NANANANANANANANANA
4.6NANANANANANANANANA
JudgementLowLowLowLowLowLowLowLowLow
5. Missing data5.1YPNYYPYPYYPYY
5.2NPNNNNPNNPNN
5.3NPYNNPNPNNPNN
5.4NANINANANANANANANA
5.5NANINANANANANANANA
JudgementLowSeriousLowLowLowLowLowLowLow
6. Measurement of outcomes6.1PNPNPNPYPYPYPNPYPY
6.2NNNNINININNINI
6.3PYPYPYPYPYNIPYNINI
6.4PNPNPNPNPNNIPNNINI
JudgementLowLowLowModerateModerateSeriousLowSeriousSerious
7. Selection of the reported result7.1PNPNPNPNPNPNPNPNPN
7.2PNPNPNPNPNPNPNPNPN
7.3PNPNPNPNPNPNPNPNPN
JudgementLowLowLowLowLowLowLowLowLow
OverallJudgementModerateSeriousSeriousSeriousModerateSeriousSeriousSeriousSerious
Y, yes; PY, probably yes; N, no; PN, probably no; NI, no information; NA, not applicable.
Table
(B)
(B)
DomainReferenceGomes
et al., 2016		Jansson
et al., 2001		Joshipura et al., 2006Liljestrand et al., 2016Lin et al., 2015Messing
et al., 2019		Pasqualini et al., 2012Petersen
et al., 2014
1. Confounding1.1PYPYPYPYPYPYPYPY
1.2NANANANANANANANA
1.3NANANANANANANANA
1.4PYPYPYPYPYPNPYPY
1.5PYPYPYPYPYPNPYPY
1.6NANININANANANANA
1.7NANANANANANANANA
1.8NANANANANANANANA
JudgementModerateModerateModerateModerateModerateSeriousModerateModerate
2. Selection of participants into the study2.1NPNPNPNNPNPNPN
2.2NANANANANANANANA
2.3NANANANANANANANA
2.4NINININININININI
2.5NANANANANANANANA
JudgementNINININININININI
3. Classification of interventions3.1PYPYPYPYPYPYPYPY
3.2PYPYPYPYPYPYPYPY
3.3NPNPNPNPNPNNPN
JudgementLowLowLowLowLowLowLowLow
4. Deviations from intended interventions4.1PNPNPNPNPNPNPNPN
4.2NANANANANANANANA
4.3NANANANANANANANA
4.4NANANANANANANANA
4.5NANANANANANANANA
4.6NANANANANANANANA
JudgementLowLowLowLowLowLowLowLow
5. Missing data5.1YPYPYPYYPYPYY
5.2NPNPNPNPNPNPNN
5.3NPNPNPNPNPNPNN
5.4NANANANANANANANA
5.5NANANANANANANANA
JudgementLowLowLowLowLowLowLowLow
6. Measurement of outcomes6.1PNPNPNPNPNPNPNPY
6.2NNINNNININNI
6.3PYPYPYPYPYPYPYPY
6.4PNPNPNPNPNPNPNPN
JudgementLowModerateLowLowModerateModerateLowModerate
7. Selection of the reported result7.1PNPNPNPNPNPNPNPN
7.2PNPNPNPNPNPNPNPN
7.3PNPNPNPNPNPNPNPN
JudgementLowLowLowLowLowLowLowLow
OverallJudgementModerateModerateModerateModerateModerateSeriousModerateModerate
Y, yes; PY, probably yes; N, no; PN, probably no; NI, no information; NA, not applicable.
Table
(C)
(C)
DomainReferenceSegura-Egea et al., 2010Virtanen et al., 2017Willershausen et al., 2009Willershausen et al., 2014
1. Confounding1.1PYPYPYPY
1.2NANANANA
1.3NANANANA
1.4PYPYPYPY
1.5PYPYPYPY
1.6NANANANA
1.7NANANANA
1.8NANANANA
JudgementModerateModerateModerateModerate
2. Selection of participants into the study2.1NPNPNPN
2.2NANANANA
2.3NANANANA
2.4NINININI
2.5NANANANA
JudgementNINININI
3. Classification of interventions3.1PYPYPYPY
3.2PYPYPYPY
3.3PYNPYPY
JudgementSeriousLowSeriousSerious
4. Deviations from intended interventions4.1PNPNPNPN
4.2NANANANA
4.3NANANANA
4.4NANANANA
4.5NANANANA
4.6NANANANA
JudgementLowLowLowLow
5. Missing data5.1YPNYPY
5.2NPNNPN
5.3NPNNPN
5.4NANINANA
5.5NANINANA
JudgementLowModerateLowLow
6. Measurement of outcomes6.1PYPNPYPY
6.2NINNINI
6.3PYPYPYPY
6.4PNPNPNPN
JudgementModerateLowModerateModerate
7. Selection of the reported result7.1PNPNPNPN
7.2PNPNPNPN
7.3PNPNPNPN
JudgementLowLowLowLow
OverallJudgementSeriousModerateSeriousSerious
Y, yes; PY, probably yes; N, no; PN, probably no; NI, no information; NA, not applicable.
Table
Table A2. Summary of Findings Table/GRADE assessment.
Table A2. Summary of Findings Table/GRADE assessment.
Association Between Chronic Endodontic Infection and Cardiovascular Disease
Patient or population: any
Settings: population studies
Exposure: chronic dental infection
Outcome: cardiovascular event
OutcomesIllustrative comparative risks * (95% CI)Relative effect
(95% CI)		No of
participants
(studies)		Quality of the
evidence
(GRADE)		Comments
Assumed riskCorresponding risk
Cardiovascular Event41 per 100056 per 1000
(43 to 73)		RR 1.38
(1.06 to 1.80)		670,668
(10 studies)		⊕⊝⊝⊝
very low 1,2		-
* The basis for the assumed risk (e.g., the median control group risk across studies) is provided in footnotes. The corresponding risk (and its 95% confidence interval) is based on the assumed risk in the comparison group and the relative effect of the exposure (and its 95% CI). CI: confidence interval; RR: risk ratio; GRADE Working Group grades of evidence. High quality: Further research is very unlikely to change our confidence in the estimate of effect. Moderate quality: Further research is likely to have an important impact on our confidence in the estimate of effect and may change the estimate. Low quality: Further research is very likely to have an important impact on our confidence in the estimate of effect and is likely to change the estimate. Very low quality: We are very uncertain about the estimate; 1 downgraded once due to heterogeneity identified. 2 downgraded due to suspected publication bias.

References

  1. Roth, G.A.; Abate, D.; Abate, K.H.; Abay, S.M.; Abbafati, C.N.; Abbasi, N.; GBD 2017 Causes of Death Collaborators. Global, Regional, and National Age-Sex-Specific Mortality for 282 Causes of Death in 195 Countries and Territories, 1980–2017: A Systematic Analysis for the Global Burden of Disease Study 2017. Lancet 2018, 392, 1736–1788. [Google Scholar] [CrossRef] [Green Version]
  2. Roth, G.A.; Johnson, C.; Abajobir, A.; Abd-Allah, F.; Abera, S.F.; Abyu, G.; Ahmed, M.; Aksut, B.; Alam, T.; Alam, K.; et al. Global, Regional, and National Burden of Cardiovascular Diseases for 10 Causes, 1990 to 2015. J. Am. Coll. Cardiol. 2017, 70, 1–25. [Google Scholar] [CrossRef] [PubMed]
  3. WHO. CVD Risk Chart Working Group World Health Organization Cardiovascular Disease Risk Charts: Revised Models to Estimate Risk in 21 Global Regions. Lancet Glob. Health 2019, 7, e1332–e1345. [Google Scholar] [CrossRef] [Green Version]
  4. Gaziano, T.A.; Abrahams-Gessel, S.; Alam, S.; Alam, D.; Ali, M.; Bloomfield, G.; Carrillo-Larco, R.M.; Dorairaj, P.; Gutierrez, L.; Irazola, V.; et al. Comparison of Nonblood-Based and Blood-Based Total CV Risk Scores in Global Populations. Glob. Heart 2016, 11, 37–46.e2. [Google Scholar] [CrossRef] [PubMed]
  5. Beck, J.D.; Pankow, J.; Tyroler, H.A.; Offenbacher, S. Dental Infections and Atherosclerosis. Am. Heart J. 1999, 138, S528–S533. [Google Scholar] [CrossRef]
  6. Beck, J.D.; Slade, G.; Offenbacher, S. Oral Disease, Cardiovascular Disease and Systemic Inflammation. Periodontology 2000, 23, 110–120. [Google Scholar] [CrossRef]
  7. Beck, J.D.; Offenbacher, S. The Association between Periodontal Diseases and Cardiovascular Diseases: A State-of-the-Science Review. Ann. Periodontol. 2001, 6, 9–15. [Google Scholar] [CrossRef] [PubMed]
  8. Sanz, M.; Del Castillo, A.M.; Jepsen, S.; Gonzalez-Juanatey, J.R.; D’Aiuto, F.; Bouchard, P.; Chapple, I.; Dietrich, T.; Gotsman, I.; Graziani, F.; et al. Periodontitis and Cardiovascular Diseases. Consensus Report. Glob. Heart 2020, 15, 1. [Google Scholar] [CrossRef] [Green Version]
  9. Tomás, I.; Diz, P.; Tobías, A.; Scully, C.; Donos, N. Periodontal Health Status and Bacteraemia from Daily Oral Activities: Systematic Review/Meta-Analysis. J. Clin. Periodontol. 2012, 39, 213–228. [Google Scholar] [CrossRef]
  10. Balejo, R.D.P.; Cortelli, J.R.; Costa, F.O.; Cyrino, R.M.; Aquino, D.R.; Cogo-Müller, K.; Miranda, T.B.; Moura, S.P.; Cortelli, S.C. Effects of Chlorhexidine Preprocedural Rinse on Bacteremia in Periodontal Patients: A Randomized Clinical Trial. J. Appl. Oral Sci. 2017, 25, 586–595. [Google Scholar] [CrossRef] [Green Version]
  11. Rafferty, B.; Jönsson, D.; Kalachikov, S.; Demmer, R.T.; Nowygrod, R.; Elkind, M.S.V.; Bush, H.; Kozarov, E. Impact of Monocytic Cells on Recovery of Uncultivable Bacteria from Atherosclerotic Lesions. J. Intern. Med. 2011, 270, 273–280. [Google Scholar] [CrossRef]
  12. Kakehashi, S.; Stanley, H.R.; Fitzgerald, R.J. The Effects of Surgical Exposures of Dental Pulps in Germ-Free and Conventional Laboratory Rats. Oral Surg. Oral Med. Oral Pathol. 1965, 20, 340–349. [Google Scholar] [CrossRef]
  13. Cotti, E.; Dessì, C.; Piras, A.; Mercuro, G. Can a Chronic Dental Infection Be Considered a Cause of Cardiovascular Disease? A Review of the Literature. Int. J. Cardiol. 2011, 148, 4–10. [Google Scholar] [CrossRef]
  14. Caplan, D.J.; Chasen, J.B.; Krall, E.A.; Cai, J.; Kang, S.; Garcia, R.I.; Offenbacher, S.; Beck, J.D. Lesions of Endodontic Origin and Risk of Coronary Heart Disease. J. Dent. Res. 2006, 85, 996–1000. [Google Scholar] [CrossRef]
  15. An, G.K.; Morse, D.E.; Kunin, M.; Goldberger, R.S.; Psoter, W.J. Association of Radiographically Diagnosed Apical Periodontitis and Cardiovascular Disease: A Hospital Records–Based Study. J. Endod. 2016, 42, 916–920. [Google Scholar] [CrossRef] [PubMed]
  16. Chauhan, N.; Mittal, S.; Tewari, S.; Sen, J.; Laller, K. Association of Apical Periodontitis with Cardiovascular Disease via Noninvasive Assessment of Endothelial Function and Subclinical Atherosclerosis. J. Endod. 2019, 45, 681–690. [Google Scholar] [CrossRef] [PubMed]
  17. Jakovljevic, A.; Duncan, H.F.; Nagendrababu, V.; Jacimovic, J.; Milasin, J.; Dummer, P.M.H. Association between Cardiovascular Diseases and Apical Periodontitis: An Umbrella Review. Int. Endod. J. 2020, 53, 1374–1386. [Google Scholar] [CrossRef] [PubMed]
  18. Messing, M.; Souza, L.C.d.; Cavalla, F.; Kookal, K.K.; Rizzo, G.; Walji, M.; Silva, R.; Letra, A. Investigating Potential Correlations between Endodontic Pathology and Cardiovascular Diseases Using Epidemiological and Genetic Approaches. J. Endod. 2019, 45, 104–110. [Google Scholar] [CrossRef]
  19. Cowan, L.T.; Lakshminarayan, K.; Lutsey, P.L.; Beck, J.; Offenbacher, S.; Pankow, J.S. Endodontic Therapy and Incident Cardiovascular Disease: The Atherosclerosis Risk in Communities (ARIC) Study. J. Public Health Dent. 2020, 80, 79–91. [Google Scholar] [CrossRef]
  20. Koletsi, D.; Iliadi, A.; Tzanetakis, G.; Vavuranakis, M.; Eliades, T. Cardiovascular Disease and Chronic Dental Infection. Is There an Association? A Systematic Review and Meta-Analysis Protocol [osf.io/mxa24]. 2020. [Google Scholar]
  21. Stroup, D.F.; Berlin, J.A.; Morton, S.C.; Olkin, I.; Williamson, G.D.; Rennie, D.; Moher, D.; Becker, B.J.; Sipe, T.A.; Thacker, S.B. Meta-Analysis of Observational Studies in Epidemiology: A Proposal for Reporting. Meta-Analysis Of Observational Studies in Epidemiology (MOOSE) Group. JAMA 2000, 283, 2008–2012. [Google Scholar] [CrossRef]
  22. Fleming, P.S.; Koletsi, D.; Pandis, N. Blinded by PRISMA: Are Systematic Reviewers Focusing on PRISMA and Ignoring Other Guidelines? PLoS ONE 2014, 9, e96407. [Google Scholar] [CrossRef]
  23. Sterne, J.A.; Hernán, M.A.; Reeves, B.C.; Savović, J.; Berkman, N.D.; Viswanathan, M.; Henry, D.; Altman, D.G.; Ansari, M.T.; Boutron, I.; et al. ROBINS-I: A Tool for Assessing Risk of Bias in Non-Randomised Studies of Interventions. BMJ 2016, 355, i4919. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Egger, M.; Davey Smith, G.; Schneider, M.; Minder, C. Bias in Meta-Analysis Detected by a Simple, Graphical Test. BMJ 1997, 315, 629–634. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Guyatt, G.H.; Oxman, A.D.; Vist, G.E.; Kunz, R.; Falck-Ytter, Y.; Alonso-Coello, P.; Schünemann, H.J.; GRADE Working Group. GRADE: An Emerging Consensus on Rating Quality of Evidence and Strength of Recommendations. BMJ 2008, 336, 924–926. [Google Scholar] [CrossRef] [Green Version]
  26. Balshem, H.; Helfand, M.; Schünemann, H.J.; Oxman, A.D.; Kunz, R.; Brozek, J.; Vist, G.E.; Falck-Ytter, Y.; Meerpohl, J.; Norris, S.; et al. GRADE Guidelines: 3. Rating the Quality of Evidence. J. Clin. Epidemiol. 2011, 64, 401–406. [Google Scholar] [CrossRef]
  27. Costa, T.H.R.; Neto, J.A.d.F.; de Oliveira, A.E.F.; e Maia, M.D.F.L.; de Almeida, A.L. Association between Chronic Apical Periodontitis and Coronary Artery Disease. J. Endod. 2014, 40, 164–167. [Google Scholar] [CrossRef] [PubMed]
  28. de Oliveira, B.P.; Cruz Câmara, A.; Aguiar, M. Prevalence of Asymptomatic Apical Periodontitis and its Association with Coronary Artery Disease in a Brazilian Subpopulation. Acta Stomatol. Croat. 2017, 51, 106–112. [Google Scholar] [CrossRef] [PubMed]
  29. Frisk, F.; Hakeberg, M.; Ahlqwist, M.; Bengtsson, C. Endodontic Variables and Coronary Heart Disease. Acta Odontol. Scand. 2003, 61, 257–262. [Google Scholar] [CrossRef]
  30. Garrido, M.; Cárdenas, A.M.; Astorga, J.; Quinlan, F.; Valdés, M.; Chaparro, A.; Carvajal, P.; Pussinen, P.; Huamán-Chipana, P.; Jalil, J.E.; et al. Elevated Systemic Inflammatory Burden and Cardiovascular Risk in Young Adults with Endodontic Apical Lesions. J. Endod. 2019, 45, 111–115. [Google Scholar] [CrossRef]
  31. Gomes, M.S.; Hugo, F.N.; Hilgert, J.B.; Sant’Ana Filho, M.; Padilha, D.M.P.; Simonsick, E.M.; Ferrucci, L.; Reynolds, M.A. Apical Periodontitis and Incident Cardiovascular Events in the Baltimore Longitudinal Study of Ageing. Int. Endod. J. 2016, 49, 334–342. [Google Scholar] [CrossRef] [Green Version]
  32. Jansson, L.; Lavstedt, S.; Frithiof, L.; Theobald, H. Relationship between Oral Health and Mortality in Cardiovascular Diseases: Oral Health and Mortality. J. Clin. Periodontol. 2001, 28, 762–768. [Google Scholar] [CrossRef]
  33. Joshipura, K.; Pitiphat, W.; Hung, H.; Willett, W.; Colditz, G.; Douglass, C. Pulpal Inflammation and Incidence of Coronary Heart Disease. J. Endod. 2006, 32, 99–103. [Google Scholar] [CrossRef] [PubMed]
  34. Liljestrand, J.M.; Mäntylä, P.; Paju, S.; Buhlin, K.; Kopra, K.A.E.; Persson, G.R.; Hernandez, M.; Nieminen, M.S.; Sinisalo, J.; Tjäderhane, L.; et al. Association of Endodontic Lesions with Coronary Artery Disease. J. Dent. Res. 2016, 95, 1358–1365. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Lin, P.-Y.; Chien, K.-L.; Chang, H.-J.; Chi, L.-Y. Unfinished Root Canal Treatments and the Risk of Cardiovascular Disease. J. Endod. 2015, 41, 1991–1996. [Google Scholar] [CrossRef]
  36. Pasqualini, D.; Bergandi, L.; Palumbo, L.; Borraccino, A.; Dambra, V.; Alovisi, M.; Migliaretti, G.; Ferraro, G.; Ghigo, D.; Bergerone, S.; et al. Association among Oral Health, Apical Periodontitis, CD14 Polymorphisms, and Coronary Heart Disease in Middle-Aged Adults. J. Endod. 2012, 38, 1570–1577. [Google Scholar] [CrossRef]
  37. Petersen, J.; Glaßl, E.-M.; Nasseri, P.; Crismani, A.; Luger, A.K.; Schoenherr, E.; Bertl, K.; Glodny, B. The Association of Chronic Apical Periodontitis and Endodontic Therapy with Atherosclerosis. Clin. Oral Investig. 2014, 18, 1813–1823. [Google Scholar] [CrossRef] [Green Version]
  38. Segura-Egea, J.J.; Jimenez-Moreno, E.; Calvo-Monroy, C.; Ríos-Santos, J.V.; Velasco-Ortega, E.; Sánchez-Domínguez, B.; Castellanos-Cosano, L.; Llamas-Carreras, J.M. Hypertension and Dental Periapical Condition. J. Endod. 2010, 36, 1800–1804. [Google Scholar] [CrossRef] [PubMed]
  39. Virtanen, E.; Nurmi, T.; Söder, P.-Ö.; Airila-Månsson, S.; Söder, B.; Meurman, J.H. Apical Periodontitis Associates with Cardiovascular Diseases: A Cross-Sectional Study from Sweden. BMC Oral Health 2017, 17, 107. [Google Scholar] [CrossRef] [PubMed]
  40. Willershausen, B.; Kasaj, A.; Willershausen, I.; Zahorka, D.; Briseño, B.; Blettner, M.; Genth-Zotz, S.; Münzel, T. Association between Chronic Dental Infection and Acute Myocardial Infarction. J. Endod. 2009, 35, 626–630. [Google Scholar] [CrossRef] [PubMed]
  41. Willershausen, I.; Weyer, V.; Peter, M.; Weichert, C.; Kasaj, A.; Münzel, T.; Willershausen, B. Association between Chronic Periodontal and Apical Inflammation and Acute Myocardial Infarction. Odontology 2014, 102, 297–302. [Google Scholar] [CrossRef]
  42. Friedlander, A.H.; Sung, E.C.; Chung, E.M.; Garrett, N.R. Radiographic Quantification of Chronic Dental Infection and Its Relationship to the Atherosclerotic Process in the Carotid Arteries. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2010, 109, 615–621. [Google Scholar] [CrossRef]
  43. Vasan, R.S.; Benjamin, E.J. The Future of Cardiovascular Epidemiology. Circulation 2016, 133, 2626–2633. [Google Scholar] [CrossRef] [Green Version]
  44. Unudurthi, S.D.; Luthra, P.; Bose, R.J.C.; McCarthy, J.R.; Kontaridis, M.I. Cardiac Inflammation in COVID-19: Lessons from Heart Failure. Life Sci. 2020, 260, 118482. [Google Scholar] [CrossRef]
  45. World Health Organization (WHO). Cardiovascular diseases. Available online: https://www.Who.Int/Cardiovascular_diseases/About_cvd/En/ (accessed on 9 September 2020).
  46. Wang, L.C.; Gu, A.Q.; Sun, C.L.; Xu, H.; Ni, X.S.; Wang, R.J.; Zhao, X.Y.; Wang, Q.C. Cross-Sectional Study of Factors Correlated to Quality of Life in Patients with Coronary Artery Disease and Diabetic Retinopathy. J. Biol. Regul. Homeost. Agents 2020, 34, 1235–1240. [Google Scholar] [PubMed]
  47. Li, S.; Tang, X.; Luo, Y.; Wu, B.; Huang, Z.; Li, Z.; Peng, L.; Ling, Y.; Zhu, J.; Zhong, J.; et al. Impact of Long-Term Glucose Variability on Coronary Atherosclerosis Progression in Patients with Type 2 Diabetes: A 2.3 Year Follow-up Study. Cardiovasc. Diabetol. 2020, 19, 146. [Google Scholar] [CrossRef] [PubMed]
  48. Parada-Turska, J.; Wójcicka, G.; Beltowski, J. Paraoxonase 1 Phenotype and Protein N-Homocysteinylation in Patients with Rheumatoid Arthritis: Implications for Cardiovascular Disease. Antioxidants 2020, 9, 899. [Google Scholar] [CrossRef]
  49. Zafar, R. An Insight into Pathogenesis of Cardiovascular Diseases. J. Integr. Cardiol. 2015, 1, 79–81. [Google Scholar] [CrossRef]
  50. Koivisto, T.; Bowles, W.R.; Rohrer, M. Frequency and Distribution of Radiolucent Jaw Lesions: A Retrospective Analysis of 9,723 Cases. J. Endod. 2012, 38, 729–732. [Google Scholar] [CrossRef] [PubMed]
  51. Braz-Silva, P.H.; Bergamini, M.L.; Mardegan, A.P.; De Rosa, C.S.; Hasseus, B.; Jonasson, P. Inflammatory Profile of Chronic Apical Periodontitis: A Literature Review. Acta Odontol. Scand. 2019, 77, 173–180. [Google Scholar] [CrossRef] [Green Version]
  52. de Oliveira Rodini, C.; Batista, A.C.; Lara, V.S. Comparative Immunohistochemical Study of the Presence of Mast Cells in Apical Granulomas and Periapical Cysts: Possible Role of Mast Cells in the Course of Human Periapical Lesions. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2004, 97, 59–63. [Google Scholar] [CrossRef]
  53. Aranha, A.M.F.; Repeke, C.E.; Garlet, T.P.; Vieira, A.E.; Campanelli, A.P.; Trombone, A.P.F.; Letra, A.; Silva, R.M.; Garlet, G.P. Evidence Supporting a Protective Role for Th9 and Th22 Cytokines in Human and Experimental Periapical Lesions. J. Endod. 2013, 39, 83–87. [Google Scholar] [CrossRef] [PubMed]
  54. Bergandi, L.; Giuggia, B.; Alovisi, M.; Comba, A.; Silvagno, F.; Maule, M.; Aldieri, E.; Scotti, N.; Scacciatella, P.; Conrotto, F.; et al. Endothelial Dysfunction Marker Variation in Young Adults with Chronic Apical Periodontitis before and after Endodontic Treatment. J. Endod. 2019, 45, 500–506. [Google Scholar] [CrossRef] [PubMed]
  55. Munz, M.; Richter, G.M.; Loos, B.G.; Jepsen, S.; Divaris, K.; Offenbacher, S.; Teumer, A.; Holtfreter, B.; Kocher, T.; Bruckmann, C.; et al. Genome-Wide Association Meta-Analysis of Coronary Artery Disease and Periodontitis Reveals a Novel Shared Risk Locus. Sci. Rep. 2018, 8, 13678. [Google Scholar] [CrossRef] [PubMed]
  56. Aminoshariae, A.; Kulild, J.C.; Fouad, A.F. The Impact of Endodontic Infections on the Pathogenesis of Cardiovascular Disease(s): A Systematic Review with Meta-Analysis Using GRADE. J. Endod. 2018, 44, 1361–1366.e3. [Google Scholar] [CrossRef] [PubMed]
  57. Berlin-Broner, Y.; Febbraio, M.; Levin, L. Association between Apical Periodontitis and Cardiovascular Diseases: A Systematic Review of the Literature. Int. Endod. J. 2017, 50, 847–859. [Google Scholar] [CrossRef] [Green Version]
  58. Khalighinejad, N.; Aminoshariae, M.R.; Aminoshariae, A.; Kulild, J.C.; Mickel, A.; Fouad, A.F. Association between Systemic Diseases and Apical Periodontitis. J. Endod. 2016, 42, 1427–1434. [Google Scholar] [CrossRef]
  59. González Navarro, B.; Pintó Sala, X.; Jané Salas, E. Relationship between Cardiovascular Disease and Dental Pathology. Systematic Review. Med. Clin. 2017, 149, 211–216. [Google Scholar] [CrossRef]
Ijerph 18 09111 g001 550
Figure 1. Flow chart of study selection.
Figure 1. Flow chart of study selection.
Ijerph 18 09111 g001
Ijerph 18 09111 g002 550
Figure 2. Forest plot for random effects meta-analyses for the overall effect of LEO (lesions of endodontic origin) and CVD (cardiovascular disease) events.
Figure 2. Forest plot for random effects meta-analyses for the overall effect of LEO (lesions of endodontic origin) and CVD (cardiovascular disease) events.
Ijerph 18 09111 g002
Ijerph 18 09111 g003 550
Figure 3. Contour-enhanced funnel plot for inspection of publication bias.
Figure 3. Contour-enhanced funnel plot for inspection of publication bias.
Ijerph 18 09111 g003
Table
Table 1. Characteristics of included studies (n = 21).
Table 1. Characteristics of included studies (n = 21).
Study ID/DesignPopulationExposureOutcomeNotes
An et al., 2016
Cross-sectional (pair-matched)		362 patients (266 female, 98 male); mean age 49AP (+/−), radiographic
diagnosis		Prevalence of CVD, HCAOther underlying disease in part of the sample:
diabetes
Caplan et al., 2006
Cohort (prospective)		708 patients (all male); age split to <45 yrs or >45 yrsLEO (+/−), radiographic diagnosisTime to diagnosis of CHDLongitudinal cohort, splits patients < or > 45 yrs; other underlying
disease in part of the
sample: diabetes
Chauhan et al., 2019
Cross-sectional (pair-matched)		120 patients (all male); 20–40 yrsAP (+/−), radiographic
diagnosis		FMD, c-IMTPhysiologic and anatomic measures of early CVD
Costa et al., 2014
Cross-sectional 		103 patients (52 male, 51 female); mean age 61.9 yrsAP (+/−), radiographic
diagnosis		Prevalence of CADOther underlying disease in part of the sample:
diabetes
Cowan et al., 2020
Cohort (population-based prospective)		6274 patients (based on CHD data) (2966 male, 3308 female); mean age 62.3 ± 5.7, for non-ET/62.7 ± 5.7 for ET casesSelf-reported history of ET Incidence of CHDOther underlying disease in part of the sample:
diabetes, periodontal
disease; ET as proxy of
infection and also
self-reported
deOliviera et al., 2017
Cross-sectional 		1346 patients (438 male, 908 female); wide age range: ≤18 and 19 to ≥60 yrsAP (+/−), radiographic
diagnosis		Prevalence of CAD
Friedlander et al., 2010
Cross-sectional (pair-matched)		72 patients (70 male, 72 female); cases mean age: 64.4 ± 10.0; controls mean age: 64.9 ± 10.1CAA (+/−)CDI as measured by MPIMPI noted as the outcome, after matching on CAA presence
Frisk et al., 2003
Cross-sectional		1056 patients (all female); mean age: 64.7 ± 10.7PA (+/−), radiographic
diagnosis		Prevalence of CVDOther underlying disease in part of the sample:
diabetes
Garrido et al., 2019
Cross-sectional		55 patients (32 male, 23 female); cases mean age: 25.9 ± 5.0; controls mean age: 24.5 ± 3.9LEO (+/−), radiographic diagnosisCVD, (hsCRP)Blood samples for other proxy markers for CVD risk as well
Gomes et al., 2016
Cohort (retrospective)		278 patients (143 male, 135 female); age: 55 ± 16.8 yrsAP (+/−), radiographic diagnosisIncidence of CHDOther underlying disease in part of the sample:
diabetes
Jansson et al., 2001
Cohort (retrospective)		1393 patients (687 male, 706 female); age range: 18–66 yrsAP (+/−), radiographic diagnosisPrevalence of CVD
(mortality)
Joshipura et al., 2006
Cohort (retrospective)		34,683 patients (male); age range: cases mean age: 54.2 ± 9.1; controls mean age: 52.1 ± 9.2Pulpal inflammation leading to RCT (+/−)Incidence of CHDSelf-reported root canal for inflammation as
surrogate; sample
consisted of health professionals
Liljestrand et al., 2016
Cross-sectional		508 patients (330 male, 178 female); age: 62.1 (±10.4)Prevalence CAD, ACSLEO (+/−), radiographic diagnosisOther underlying disease in part of the sample:
diabetes; no clear
breakdown of LEOs
Lin et al., 2015
Cohort (population-based retrospective)		283,590 participants (123804 male, 159786 female); age range: 20- >60Unfinished RCT (+/−)First diagnosis of CVD hospitalizationOther underlying disease in part of the sample:
diabetes
Messing et al., 2019
Cross-sectional		666.768 patients; age range: 18–65EP (+/−),Prevalence all CVDs
combined (self-reported)		Epidemiologic association plus genetic association study
Pasqualini et al., 2012
Case-control		100 patients; cases age:
48 ± 5.7; controls age:
47 ± 7.1		Acute myocardial
infraction/unstable
angina		LEO (+/−), clinical, and
radiographic		Identification of
polymorphisms CD14
Petersen et al., 2014
Cross-sectional		531 patients (1191 teeth); age 50 ± 15.7AP (+/−), radiographic diagnosis CT scanVolume of aortic atherosclerotic burdenLarge number of
subgroups
Segura-Egea et al., 2010
Cross-sectional		91 patients (21.2 teeth/patient); cases age:
59.5 ± 9.7; controls age: 56.4 ± 9.9		Hypertension (+/−)AP (+/−), radiographic
diagnosis		Underlying diseases
(diabetes, smoke) not
recorded in the sample
Virtanen et al., 2017
Cross-sectional		120 patients (57 male, 63 female); cases age:
53.0 ± 2.7; controls age: 51.4 ± 2.9		AP (+), radiographic
diagnosis		Prevalence of CVDMost CVD cases were classified as hypertensive (no specific number
provided)
Willershausen et al., 2009
Cross-sectional		250 patients (203 male, 47 female); cases age:
61.8 ± 10.4; controls age: 63.4 ± 10.7		Myocardial infraction (+/−)Prevalence of LEO (+/−)Other underlying disease in part of the sample:
diabetes
Willershausen et al., 2014
Cross-sectional		497 patients; mean age 62.3, range 51–83Acute myocardial
infraction (+/−)		Prevalence of LEO (+/−)Lesions of periodontal origin also recorded
ACS, acute coronary syndrome; AP, apical periodontitis; CAA, coronary artery atherosclerosis; CAD, coronary artery disease; CHD, coronary heart disease; CDI, chronic dental infection; c-IMT, intima-media thickness; CT, computed tomography; CVD, cardiovascular disease; EP, endodontic pathology; ET, endodontic treatment; FMD, flow-mediated dilatation; HCA hypercholesterolemia; hsCRP, high-sensitivity C-reactive protein; LEO, lesions of endodontic origin; MPI, Mattila pantomography index; PA, periapical destruction; yrs, years; RCT, root canal treatment.
Table
Table 2. Risk of bias according to the ROBINS-I tool for the included studies (n = 21).
Table 2. Risk of bias according to the ROBINS-I tool for the included studies (n = 21).
Bias Due to/in…
ConfoundingSelection of Participants into the StudyClassification of InterventionsDeviations from Intended InterventionsMissing DataMeasurement of OutcomesSelection of the Reported ResultOverall
An et al., 2016 ModerateNo InformationLowLowLowLowLowModerate
Caplan et al., 2006 LowNo InformationLowLowSeriousLowLowSerious
Chauhan et al., 2019 SeriousNo InformationLowLowLowLowLowSerious
Costa et al., 2014 ModerateNo InformationSeriousLowLowModerateLowSerious
Cowan et al., 2020 LowLowLowLowLowModerateLowModerate
deOliviera et al., 2017 SeriousNo InformationSeriousLowLowSeriousLowSerious
Friedlander et al., 2010 SeriousNo InformationLowLowLowLowLowSerious
Frisk et al., 2003 ModerateNo InformationSeriousLowLowSeriousLowSerious
Garrido et al., 2019 ModerateNo InformationSeriousLowLowSeriousLowSerious
Gomes et al., 2016 ModerateNo InformationLowLowLowLowLowModerate
Jansson et al., 2001 ModerateNo InformationLowLowLowModerateLowModerate
Joshipura et al., 2006 ModerateNo InformationLowLowLowLowLowModerate
Liljestrand et al., 2016 ModerateNo InformationLowLowLowLowLowModerate
Lin et al., 2015 ModerateNo InformationLowLowLowModerateLowModerate
Messing et al., 2019 SeriousNo InformationLowLowLowModerateLowSerious
Pasqualini et al., 2012 ModerateNo InformationLowLowLowLowLowModerate
Petersen et al., 2014 ModerateNo InformationLowLowLowModerateLowModerate
Segura-Egea et al., 2010 ModerateNo InformationSeriousLowLowModerateLowSerious
Virtanen et al., 2017 ModerateNo InformationLowLowModerateLowLowModerate
Willershausen et al., 2009 ModerateNo InformationSeriousLowLowModerateLowSerious
Willershausen et al., 2014 ModerateNo InformationSeriousLowLowModerateLowSerious
Table
Table 3. Results of meta-analyses, sensitivity analyses.
Table 3. Results of meta-analyses, sensitivity analyses.
SynthesisNo. StudiesRisk Ratio95%CIsp-ValueI2 (%)Tau-Squared
Overall101.381.06, 1.80<0.00192.20.13
Age 181.461.19, 1.79<0.00170.60.05
Matching 281.361.02, 1.820.0493.50.15
Self-reporting 391.461.21, 1.77<0.00166.40.05
All-sensitivity 461.461.15, 1.860.00279.00.07
Bias 541.861.37, 2.52<0.00150.70.05
1 two studies with a wide age range (including patients less than 45 years of age) have been excluded. 2 two studies with unequal distribution of important potential confounders (i.e., diabetes, smoking) across sample groups have been excluded. 3 one study has been excluded due to assessment based on patient self-reporting for the cardiovascular outcome. 4 four studies have been excluded for all the above reasons cumulatively. 5 four studies with moderate risk of bias were included; the remaining six with serious risk of bias have been excluded.
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Koletsi, D.; Iliadi, A.; Tzanetakis, G.N.; Vavuranakis, M.; Eliades, T. Cardiovascular Disease and Chronic Endodontic Infection. Is There an Association? A Systematic Review and Meta-Analysis. Int. J. Environ. Res. Public Health 2021, 18, 9111. https://doi.org/10.3390/ijerph18179111

AMA Style

Koletsi D, Iliadi A, Tzanetakis GN, Vavuranakis M, Eliades T. Cardiovascular Disease and Chronic Endodontic Infection. Is There an Association? A Systematic Review and Meta-Analysis. International Journal of Environmental Research and Public Health. 2021; 18(17):9111. https://doi.org/10.3390/ijerph18179111

Chicago/Turabian Style

Koletsi, Despina, Anna Iliadi, Giorgos N. Tzanetakis, Manolis Vavuranakis, and Theodore Eliades. 2021. "Cardiovascular Disease and Chronic Endodontic Infection. Is There an Association? A Systematic Review and Meta-Analysis" International Journal of Environmental Research and Public Health 18, no. 17: 9111. https://doi.org/10.3390/ijerph18179111

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