Differences in the Effects of Broad-Band UVA and Narrow-Band UVB on Epidermal Keratinocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and UV Irradiation
2.2. MTT Phototoxicity Test
2.3. ROS Measurement
2.4. Mitochondrial Membrane Potential Measurement
2.5. Comet Assay
2.6. Statistical Analysis
3. Results
3.1. UVA and UVB Phototoxicity
3.2. ROS Production
3.3. Mitochondrial Membrane Potential
3.4. DNA Damage
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dose (J/cm2) | DNA in Head (%) Mean ± SD Median (25%; 75% Percentile) | DNA in Tail (%) Mean ± SD Median (25%; 75% Percentile) | Olive Tail Moment Mean ± SD Median (25%; 75% Percentile) | ||||
---|---|---|---|---|---|---|---|
control | 0 | 93.78 ± 3.11 | 6.22 ± 3.11 | 0.97 ± 0.47 | |||
93.67 (91.67; 95.63) | 6.33 (4.37; 8.32) | 1.02 (0.71; 1.29) | |||||
UVA | 0.8 | 91.57 ± 5.82 | p < 0.05 | 8.43 ± 5.82 | p < 0.05 | 1.13 ± 1.30 | p = 0.061 |
92.34 (89.38; 94.71) | p < 0.05 | 7.66 (5.29; 10.61) | p < 0.05 | 1.02 (0.66; 1.31) | p = 0.760 | ||
4.1 | 92.56 ± 6.28 | p < 0.05 | 7.44 ± 6.28 | p < 0.05 | 1.17 ± 1.47 | p < 0.05 | |
93.05 (90.46; 96.03) | p < 0.05 | 6.95 (3.97; 9.54) | p < 0.05 | 1.07 (0.66; 1.41) | p = 0.171 | ||
16.3 | 91.88 ± 13.63 | p < 0.05 | 8.12 ± 13.63 | p < 0.05 | 2.27 ± 5.42 | p < 0.05 | |
95.61 (91.44; 99.97) | p < 0.05 | 4.39 (0.05; 8.62) | p < 0.05 | 0.91 (0.01; 1.49) | p = 0.113 | ||
UVB | 0.4 | 89.37 ± 8.89 | p < 0.05 | 10.63 ± 8.89 | p < 0.05 | 1.84 ± 2.54 | p < 0.05 |
91.07 (87.23; 94.52) | p < 0.05 | 8.93 (5.48; 12.77) | p < 0.05 | 1.28 (0.89; 1.80) | p < 0.05 | ||
2.1 | 61.31 ± 19.10 | p < 0.05 | 38.69 ± 19.10 | p < 0.05 | 10.70 ± 6.28 | p < 0.05 | |
63.04 (47.37; 76.36) | p < 0.05 | 36.96 (23.64; 52.63) | p < 0.05 | 10.14 (6.21; 14.71) | p < 0.05 | ||
8.4 | 64.88 ± 14.64 | p < 0.05 | 35.12 ± 14.64 | p < 0.05 | 8.68 ± 4.02 | p < 0.05 | |
64.82 (54.67; 75.95) | p < 0.05 | 35.18 (24.05; 45.33) | p < 0.05 | 8.32 (5.71; 10.97) | p < 0.05 |
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Bajgar, R.; Moukova, A.; Chalupnikova, N.; Kolarova, H. Differences in the Effects of Broad-Band UVA and Narrow-Band UVB on Epidermal Keratinocytes. Int. J. Environ. Res. Public Health 2021, 18, 12480. https://doi.org/10.3390/ijerph182312480
Bajgar R, Moukova A, Chalupnikova N, Kolarova H. Differences in the Effects of Broad-Band UVA and Narrow-Band UVB on Epidermal Keratinocytes. International Journal of Environmental Research and Public Health. 2021; 18(23):12480. https://doi.org/10.3390/ijerph182312480
Chicago/Turabian StyleBajgar, Robert, Anna Moukova, Nela Chalupnikova, and Hana Kolarova. 2021. "Differences in the Effects of Broad-Band UVA and Narrow-Band UVB on Epidermal Keratinocytes" International Journal of Environmental Research and Public Health 18, no. 23: 12480. https://doi.org/10.3390/ijerph182312480