Solid Anorganic Particles and Chronic Rhinosinusitis: A Histopathology Study
Abstract
:1. Introduction
1.1. Definition and Difference between Fine and Ultrafine (Micro- and Nanosized) Particles
1.2. Possible Role of Solid Anorganic Particles in Chronic Rhinosinusitis
1.3. Clinical Research of MPs and NPs and the Aim of the Study
2. Materials and Methods
- -
- Age over 18 years;
- -
- Clinical diagnosis of CRS according to EPOS2012 criteria (the criteria are identical to criteria named in EPOS2020);
- -
- Endoscopically verified hypertrophy of inferior turbinates;
- -
- Insufficient response to conservative therapy (nasal corticosteroid spray administration for 6 months or longer), as subjectively assessed by the patient;
- -
- No history of turbinate surgery and/or sinonasal tumor;
- -
- Immunocompetency and aptitude for surgery under general anaesthesia;
- -
- Written informed consent obtained.
- -
- Age over 18 years.
- -
- No history of chronic inflammatory disease of sinonasal mucosa (i.e., CRS, allergic fungal rhinosinusitis, allergic rhinitis) or other significant pathologies and surgical procedures in sinonasal region during their life (for example sinonasal tumors);
- -
- No endoscopic signs of inferior turbinate hypertrophy.
2.1. Sample Acquisition and Preparation
2.2. Raman Microspectroscopy
2.3. Histological Examination
- 0
- Normal histology (no inflammation);
- 1
- Epithelial hyperplasia;
- 2
- Epithelial hyperplasia with mild signs of inflammation;
- 3
- Chronic inflammation.
2.4. Correlation of Detected Compounds, Histology and Smoking and Occupational History
3. Results
3.1. Detected Compounds
3.2. Histology
3.3. Correlation of Histology and Detected Compounds
3.4. Correlation of Smoking Status and Detected Compounds
3.5. Correlation of Occupational History and Detected Compounds
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Sex | Age | Smoking Status | Occupation | Detected Compounds | Histology (Severity of Inflammation) |
---|---|---|---|---|---|---|
M11 | m | 42 | Y | builder (M) | GR | 0 |
M32 | m | 31 | N | administrator (O) | AC, Al comp., ankerite | 0 |
M1 | m | 78 | N | welder (M) | AC, ankerite, CaCO3, GR, TiO2-A | 1 |
M2 | m | 38 | N | programmer (O) | CaCO3, Fe3O4, TiO2-A, TiO2-R | 1 |
M3 | m | 38 | N | welder (M) | AC, CaSO4, Fe3O4, GR, TiO2-A | 1 |
M4 | m | 65 | Y | policeman (M) | AC, ankerite, GR, TiO2-A, TiO2-R | 1 |
M14 | m | 32 | N | policeman (M) | AC, Fe2O3, GR, TiO2-A | 1 |
M16 | m | 44 | Y | driver (M) | CaCO3, GR, TiO2-A, TiO2-R | 1 |
M17 | f | 42 | N | warehouse keeper (M) | ankerite, BaSO4, TiO2-A | 1 |
M18 | m | 34 | Y | carrier (M) | AC, CaCO3, GR, SiO2, TiO2-A | 1 |
M20 | m | 35 | Y | tinsmith (M) | ankerite, CaCO3, GR, SiO2 | 1 |
M33 | m | 43 | N | machinist (M) | AC, GR, TiO2-A | 1 |
M35 | f | 65 | N | manager (O) | CaCO3, TiO2-R | 1 |
M40 | m | 54 | N | bailiff (O) | - | 1 |
M8 | m | 44 | Y | labourer (M) | CaCO3, GR | 2 |
M10 | m | 37 | N | manager (O) | GR, TiO2-A | 2 |
M21 | m | 31 | N | labourer (M) | ankerite, GR | 2 |
M2 | m | 28 | N | rolling mill operator (M) | TiO2-A | 2 |
M25 | m | 44 | N | executive director (O) | AC, Si comp., TiO2-A, TiO2-R | 2 |
M28 | m | 42 | Y | policeman (M) | AC, ankerite, BaSO4, GR | 2 |
M29 | m | 25 | Y | unemployed (O) | AC, GR, TiO2-A | 2 |
M30 | m | 39 | Y | welder (M) | AC, Al comp., (Ca, Mg)CO3 | 2 |
M5 | f | 26 | N | student (O) | Fe3O4, GR | 3 |
M6 | f | 44 | N | labourer (M) | AC, CaCO3 | 3 |
M7 | m | 28 | N | student (O) | GR | 3 |
M9 | f | 58 | N | artist (O) | GR, TiO2-A | 3 |
M12 | f | 45 | N | manager (O) | GR, TiO2-A | 3 |
M13 | m | 40 | N | clerk (O) | Fe2O3, TiO2-A | 3 |
M15 | f | 53 | N | shop assistant (M) | ankerite, GR, TiO2-A | 3 |
M19 | m | 48 | N | waiter (M) | ankerite, CaCO3, Fe2O3, SiO2, TiO2-A | 3 |
M23 | m | 28 | N | operator (M) | TiO2-A | 3 |
M24 | m | 54 | N | policeman (M) | AC, GR | 3 |
M26 | m | 42 | N | train dispatcher (M) | AC, CaCO3, GR | 3 |
M27 | m | 25 | N | student (O) | AC | 3 |
M31 | f | 20 | N | student (O) | GR, TiO2-A, TiO2-R | 3 |
M34 | f | 41 | N | seamstress (M) | GR, TiO2-A | 3 |
M36 | m | 36 | N | IT technician (O) | GR, TiO2-A | 3 |
M37 | m | 44 | N | production supervisor (M) | - | 3 |
M38 | m | 34 | N | electrotechnician (M) | - | 3 |
M39 | m | 55 | N | businessman (O) | - | 3 |
Sample | Sex | Age | Smoking Status | Occupation | Detected Compounds |
---|---|---|---|---|---|
R1 | m | 71 | N | M | - |
R5 | m | 57 | Y | M | - |
R6 | m | 77 | N | M | TiO2 |
R7 | f | 78 | N | O | AC |
R8 | m | 44 | N | M | - |
R9 | m | 67 | Y | M | - |
R10 | m | 37 | Y | M | - |
R11 | f | 87 | N | O | - |
R12 | f | 84 | N | O | |
R13 | f | 84 | N | O | - |
Detected Compound | Number of Samples/40 | Percent of Samples |
---|---|---|
Graphite TiO2 amorphous carbon | 24 | 60.0% |
23 | 57.5% | |
15 | 37.5% | |
CaCO3 Ca(Fe, Mg, Mn)(CO3)2 | 10 | 25.0% |
9 | 22.5 % | |
iron compounds | 6 | 15.0% |
Severity of Inflammation | Number of Samples/40 | Percent of Samples |
---|---|---|
0 (no inflammation) 1 (epithelial hyperplasia) 2 (mild signs of inflammation) | 2 | 5.0% |
13 | 32.5% | |
7 | 17.5% | |
3 (chronic inflammation) | 18 | 45.0% |
Detected Compound | Group A/22 Samples | Group B/18 Samples | ||
---|---|---|---|---|
Number of Samples | Percent of Samples | Number of Samples | Percent of Samples | |
graphite | 14 | 63.0% | 9 | 50.0% |
TiO2 | 14 | 63.0% | 9 | 50.0% |
amorphous carbon | 7 | 31.8% | 4 | 22.2% |
CaCO3 | 7 | 31.8% | 3 | 16.7% |
Ca(Fe, Mg, Mn)(CO3)2 | 7 | 31.8% | 2 | 11.1% |
iron compounds | 3 | 13.6% | 3 | 16.7% |
Detected Compound | Smokers/9 Samples | Non-Smokers/31 Samples | ||
---|---|---|---|---|
Number of Samples | Percent of Samples | Number of Samples | Percent of Samples | |
graphite | 8 | 88.9% | 16 | 51.6% |
TiO2 | 4 | 44.4% | 19 | 61.3% |
amorphous carbon | 5 | 55.6% | 10 | 32.3% |
CaCO3 | 4 | 44.4% | 6 | 19.4% |
Ca(Fe, Mg, Mn)(CO3)2 | 3 | 33.3% | 6 | 19.4% |
iron compounds | 0 | 0.0% | 6 | 19.4% |
Detected Compound | Manual Workers/22 Samples | Office Workers/18 Samples | ||
---|---|---|---|---|
Number of Samples | Percent of Samples | Number of Samples | Percent of Samples | |
graphite | 17 | 77.3% | 7 | 38.9% |
TiO2 | 13 | 59.1% | 10 | 55.5% |
amorphous carbon | 11 | 50.0% | 4 | 22.2% |
CaCO3 | 8 | 36.4% | 2 | 11.1% |
Ca(Fe, Mg, Mn)(CO3)2 | 8 | 36.4% | 1 | 5.6% |
iron compounds | 3 | 14.6% | 2 | 11.1% |
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Čábalová, L.; Čabanová, K.; Bielniková, H.; Kukutschová, J.; Dvořáčková, J.; Zeleník, K.; Komínek, P. Solid Anorganic Particles and Chronic Rhinosinusitis: A Histopathology Study. Int. J. Environ. Res. Public Health 2022, 19, 7269. https://doi.org/10.3390/ijerph19127269
Čábalová L, Čabanová K, Bielniková H, Kukutschová J, Dvořáčková J, Zeleník K, Komínek P. Solid Anorganic Particles and Chronic Rhinosinusitis: A Histopathology Study. International Journal of Environmental Research and Public Health. 2022; 19(12):7269. https://doi.org/10.3390/ijerph19127269
Chicago/Turabian StyleČábalová, Lenka, Kristina Čabanová, Hana Bielniková, Jana Kukutschová, Jana Dvořáčková, Karol Zeleník, and Pavel Komínek. 2022. "Solid Anorganic Particles and Chronic Rhinosinusitis: A Histopathology Study" International Journal of Environmental Research and Public Health 19, no. 12: 7269. https://doi.org/10.3390/ijerph19127269