Sub-Inhibitory Concentrations of Amoxicillin and Tylosin Affect the Biofilm Formation and Virulence of Streptococcus suis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Approval Information
2.2. Bacterial Strains and Antibiotics
2.3. Determination of MIC and MBC
2.4. Biofilm Formation Assay
2.5. Growth Curve
2.6. Confocal Laser Scanning Microscopy (CLSM)
2.7. LD50 Assay
2.8. Detection of Bacterial Viscera Distribution in Mice
2.9. Histological Examination
2.10. RNA Extraction and Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
2.11. Statistical Analysis
3. Results
3.1. MIC and MBC of Amoxicillin and Tylosin for S. suis HA9801
3.2. Effect of Sub-MIC Amoxicillin and Tylosin on Biofilm Formation
3.3. Growth Curve
3.4. Confocal Laser Scanning Microscopy (CLSM)
3.5. LD50 Assay
3.6. Bacterial Distribution of Amoxicillin and Tylosin at Subminimum Inhibitory Concentrations
3.7. Histological Analysis
3.8. Expression Profiling of Virulence Genes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Antibiotics | MIC (μg mL−1) | MBC (μg mL−1) |
---|---|---|
Amoxicillin | 0.3125 | 2.5 |
Tylosin | 1.25 | 1.25 |
Group | CFU Per Mouse | Mortality | LD50/(CFU) | ||||||
---|---|---|---|---|---|---|---|---|---|
WT | 2.587 × 107 | 3.014 × 106 | 2.601 × 105 | 3.532 × 104 | 9/10 | 8/10 | 5/10 | 3/10 | 2.245 × 106 |
1/4MIC Amoxicillin | 2.313 × 1010 | 2.141 × 109 | 2.162 × 108 | 3.142 × 107 | 8/10 | 6/10 | 5/10 | 4/10 | 2.198 × 108 |
1/4MIC Tylosin | 2.121 × 1010 | 3.012 × 109 | 2.145 × 108 | 3.114 × 107 | 9/10 | 8/10 | 4/10 | 4/10 | 1.763 × 108 |
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Zuo, J.; Fan, Q.; Li, J.; Liu, B.; Xue, B.; Zhang, X.; Yi, L.; Wang, Y. Sub-Inhibitory Concentrations of Amoxicillin and Tylosin Affect the Biofilm Formation and Virulence of Streptococcus suis. Int. J. Environ. Res. Public Health 2022, 19, 8359. https://doi.org/10.3390/ijerph19148359
Zuo J, Fan Q, Li J, Liu B, Xue B, Zhang X, Yi L, Wang Y. Sub-Inhibitory Concentrations of Amoxicillin and Tylosin Affect the Biofilm Formation and Virulence of Streptococcus suis. International Journal of Environmental Research and Public Health. 2022; 19(14):8359. https://doi.org/10.3390/ijerph19148359
Chicago/Turabian StyleZuo, Jing, Qingying Fan, Jinpeng Li, Baobao Liu, Bingqian Xue, Xiaoling Zhang, Li Yi, and Yang Wang. 2022. "Sub-Inhibitory Concentrations of Amoxicillin and Tylosin Affect the Biofilm Formation and Virulence of Streptococcus suis" International Journal of Environmental Research and Public Health 19, no. 14: 8359. https://doi.org/10.3390/ijerph19148359