Characterization and Degradation Pathways of Microbacterium resistens MZT7, A Novel 17β-Estradiol-Degrading Bacterium
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Cultures Medium
2.2. Enrichment, Isolation, and Identification of E2 Degrading Bacteria
2.3. Genome Sequencing
2.4. Biodegradation Characteristics of E2 by Strain MZT7
2.5. Determination of Residual E2
2.6. Extraction and Detection of E2 and Its Metabolites
3. Results and Discussion
3.1. Isolation and Identification of Strain MZT7
3.2. Genome Characteristics and Database Analysis Related to E2 Degradation
3.3. Effect of pH, Temperature, Salinity, and Inoculum Amount on E2 Biodegradation
3.4. Effect of Carbon Source and Nitrogen Source on E2 Biodegradation
3.5. Biodegradation Products and Metabolic Pathways
3.6. Genome Annotation Reveals Biodegradation Characteristics and Pathways
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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ID | Formula | RT a (min) | m/z b | Exact Mass |
---|---|---|---|---|
E2 | C18H24O2 | 12.52 | 273.2530 | 272.1776 |
P1 (E1) | C18H22O2 | 13.07 | 271.1686 | 270.162 |
P2 (E3) | C18H24O3 | 9.12 | 288.1493 | 288.1725 |
P3 | C9H10O4 | 13.03 | 182.0579 | 182.0579 |
P4 | C9H10O | 1.22 | 167.0126 | 166.063 |
P5 | C19H28O2 | 13.49 | 269.2105 | 288.2089 |
P6 | C19H24O2 | 7.81 | 284.1853 | 284.1776 |
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Hao, P.; Wu, S.; Zhang, X.; Gou, C.; Wang, Y.; Wang, L.; Zhu, Y.; Basang, W.; Gao, Y. Characterization and Degradation Pathways of Microbacterium resistens MZT7, A Novel 17β-Estradiol-Degrading Bacterium. Int. J. Environ. Res. Public Health 2022, 19, 11097. https://doi.org/10.3390/ijerph191711097
Hao P, Wu S, Zhang X, Gou C, Wang Y, Wang L, Zhu Y, Basang W, Gao Y. Characterization and Degradation Pathways of Microbacterium resistens MZT7, A Novel 17β-Estradiol-Degrading Bacterium. International Journal of Environmental Research and Public Health. 2022; 19(17):11097. https://doi.org/10.3390/ijerph191711097
Chicago/Turabian StyleHao, Peng, Sicheng Wu, Xiqing Zhang, Changlong Gou, Yuqiong Wang, Lixia Wang, Yanbin Zhu, Wangdui Basang, and Yunhang Gao. 2022. "Characterization and Degradation Pathways of Microbacterium resistens MZT7, A Novel 17β-Estradiol-Degrading Bacterium" International Journal of Environmental Research and Public Health 19, no. 17: 11097. https://doi.org/10.3390/ijerph191711097