Liposomes Containing Amaranth Unsaponifiable Matter and Soybean Lunasin Suppress ROS Production in Fibroblasts and Reduced Interleukin Production in Macrophages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Material
2.2. Methods
2.2.1. Liposomes Preparation
2.2.2. Cell Culture Procedures
2.2.3. Thiazolyl Blue Tetrazolium Bromide (MTT) Cell Viability Assay
2.2.4. Cellular Antioxidant Activity (CAA)
2.2.5. Cytokines Expression in Mouse Peritoneal Macrophages
2.2.6. Cytokines Expression in RAW 264.7
2.2.7. Cytokines Interleukin (IL)-6 and Tumor Necrosis Factor (TNF)-α
2.2.8. Statistical Analysis
3. Results and Discussion
3.1. Liposomes Decreased the Reactive Oxygen Species (ROS) Expression in CHON-002 Fibroblasts When Cellular Antioxidant Capacity Was Assessed
3.2. UM + LunLip Inhibited the Production of Pro-Inflammatory Markers in RAW 264.7 and Peritoneal Macrophages
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dávila-Ortiz, G.; Castañeda-Reyes, E.D.; Juárez-Palomo, C.I.; Perea-Flores, M.d.J.; Pérez-Pastén-Borja, R.; Márquez-Flores, Y.K.; González de Mejía, E. Liposomes Containing Amaranth Unsaponifiable Matter and Soybean Lunasin Suppress ROS Production in Fibroblasts and Reduced Interleukin Production in Macrophages. Int. J. Environ. Res. Public Health 2022, 19, 11678. https://doi.org/10.3390/ijerph191811678
Dávila-Ortiz G, Castañeda-Reyes ED, Juárez-Palomo CI, Perea-Flores MdJ, Pérez-Pastén-Borja R, Márquez-Flores YK, González de Mejía E. Liposomes Containing Amaranth Unsaponifiable Matter and Soybean Lunasin Suppress ROS Production in Fibroblasts and Reduced Interleukin Production in Macrophages. International Journal of Environmental Research and Public Health. 2022; 19(18):11678. https://doi.org/10.3390/ijerph191811678
Chicago/Turabian StyleDávila-Ortiz, Gloria, Erick Damian Castañeda-Reyes, Carlos Ignacio Juárez-Palomo, María de Jesús Perea-Flores, Ricardo Pérez-Pastén-Borja, Yazmín Karina Márquez-Flores, and Elvira González de Mejía. 2022. "Liposomes Containing Amaranth Unsaponifiable Matter and Soybean Lunasin Suppress ROS Production in Fibroblasts and Reduced Interleukin Production in Macrophages" International Journal of Environmental Research and Public Health 19, no. 18: 11678. https://doi.org/10.3390/ijerph191811678