Solid-State Fermentation of Chestnut Shells and Effect of Explanatory Variables in Predictive Saccharification Models
Abstract
:1. Introduction
2. Materials and Methods
2.1. Pretreatment of Chestnut Shells by Fungal Solid-State Fermentation
2.2. Enzyme Assays and CNS Fiber Determination
2.3. Enzymatic Saccharification of Chestnut Shells
2.4. Data Processing and Statistical Analysis
3. Results and Discussion
3.1. Fungal Pretreatment of Chestnut Shells: Oxidative and Hydrolytic Activities
3.2. Saccharification of Pretreated Chestnut Shells
3.3. Relationship between CNS Saccharification and Explanatory Variables Using Multiple Linear Regression Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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NDF | ADF | ADL | Cellulose (a) | Hemic. | Lignin (b) | a/b Ratio | |
---|---|---|---|---|---|---|---|
Control | 82.7 | 74.2 | 38.8 | 35.5 | 8.4 | 38.8 | 0.9 |
I. lacteus | 83.7 | 78.7 | 34.0 | 44.7 | 5.0 | 33.7 | 1.3 |
G. resinaceum | 89.8 | 82.7 | 46.4 | 36.3 | 7.1 | 46.4 | 0.8 |
B. adusta | 86.0 | 80.3 | 39.0 | 41.3 | 5.8 | 39.0 | 1.1 |
P. rufa | 90.5 | 84.0 | 44.6 | 39.4 | 6.5 | 44.6 | 0.9 |
T. versicolor | 86.6 | 82.3 | 38.9 | 43.4 | 4.3 | 38.9 | 1.1 |
Trametes sp. | 90.5 | 83.2 | 41.0 | 42.3 | 7.2 | 41.0 | 1.0 |
Model 1 | Regression Model | R2 | Significance |
---|---|---|---|
A | 0.995 | p < 0.0001 | |
B | 0.791 | p < 0.0001 | |
C 2 | 0.876 | p < 0.0001 |
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Pinto, P.A.; Bezerra, R.M.F.; Fraga, I.; Amaral, C.; Sampaio, A.; Dias, A.A. Solid-State Fermentation of Chestnut Shells and Effect of Explanatory Variables in Predictive Saccharification Models. Int. J. Environ. Res. Public Health 2022, 19, 2572. https://doi.org/10.3390/ijerph19052572
Pinto PA, Bezerra RMF, Fraga I, Amaral C, Sampaio A, Dias AA. Solid-State Fermentation of Chestnut Shells and Effect of Explanatory Variables in Predictive Saccharification Models. International Journal of Environmental Research and Public Health. 2022; 19(5):2572. https://doi.org/10.3390/ijerph19052572
Chicago/Turabian StylePinto, Paula A., Rui M. F. Bezerra, Irene Fraga, Carla Amaral, Ana Sampaio, and Albino A. Dias. 2022. "Solid-State Fermentation of Chestnut Shells and Effect of Explanatory Variables in Predictive Saccharification Models" International Journal of Environmental Research and Public Health 19, no. 5: 2572. https://doi.org/10.3390/ijerph19052572