The Role of Slurry Reflux in a Corn Stalk Continuous Anaerobic Digestion System: Performance and Microbial Community
Abstract
:1. Introduction
2. Materials and Methods
2.1. Substrates and Inoculum
2.2. Experimental Digester and Setup
2.3. Analytical Techniques and Statistical Method
3. Results
3.1. Influence of Slurry Reflux on Methane Production
3.2. Influence of Slurry Reflux on System Stability
3.3. Microbiological Analysis during Biogas Production Process
3.3.1. Dynamics of Microbial Community Richness and Diversity
Dynamics of Bacterial Populations
Dynamics of Archaeal Populations at the Phylum and Genus Level
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameters | Corn Stalk | Inoculum |
---|---|---|
TS (%w/w) | 90.23 ± 0.07 | 17.46 ± 0.12 |
VS (%w/w) | 84.62 ± 0.04 | 6.12 ± 0.08 |
C (%w/w) | 42.75 ± 0.21 | 11.32 ± 0.14 |
N (%w/w) | 0.75 ± 0.07 | 0.77 ± 0.09 |
pH | - | 7.60 ± 0.1 |
Parameters | Phase Ⅰ | Phase Ⅱ | Phase Ⅲ | |||
---|---|---|---|---|---|---|
R1 | R2 | R1 | R2 | R1 | R2 | |
Time (d) | 11–37 | 38–64 | 65–91 | |||
Organic load rate (gTS/(Lreactor·d)) | 2.0 | 2.0 | 3.0 | 3.0 | 4.0 | 4.0 |
Average methane production rate (mL/gVS) | 202.35 | 260.25 | 154.80 | 209.95 | 118.70 | 202.36 |
Average volumetric methane production rate (L/(Lreactor.d)) | 1.03 | 1.32 | 1.18 | 1.60 | 1.20 | 2.05 |
Cumulative methane production (L) | 74.00 | 95.10 | 84.88 | 115.12 | 86.76 | 147.92 |
Groups | R1-1 | R1-3 | R2-1 | R2-2 | R2-3 | R2-4 | |
---|---|---|---|---|---|---|---|
Bacterial | Seq num | 50,509 | 58,538 | 55,398 | 43,665 | 22,254 | 40,155 |
OTUs | 1227 | 1133 | 1284 | 1105 | 1116 | 1354 | |
Ace | 1494.53 | 1386.81 | 1468.03 | 1434.84 | 1992.30 | 1705.42 | |
Shannon | 4.59 | 4.54 | 4.55 | 4.55 | 4.26 | 3.98 | |
Chao1 | 1406.35 | 1332.23 | 1414.02 | 1357.67 | 1514.35 | 1543.50 | |
Archaea | Seq num | 35,502 | 72,156 | 66,784 | 79,634 | 59,710 | 66,585 |
OTUs | 141 | 219 | 150 | 159 | 108 | 212 | |
Ace | 271.08 | 420.89 | 294.22 | 234.75 | 273.60 | 356.38 | |
Shannon | 1.59 | 2.21 | 1.63 | 1.92 | 1.95 | 2.02 | |
Chao1 | 220.50 | 304.00 | 211.22 | 209.32 | 174.00 | 277.54 |
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Zhao, L.; Gao, Y.; Sun, J.; Wang, Y.; Wang, C.; Yu, S.; Wang, Z.; Li, J.; Liu, R.; Kou, W. The Role of Slurry Reflux in a Corn Stalk Continuous Anaerobic Digestion System: Performance and Microbial Community. Int. J. Environ. Res. Public Health 2023, 20, 1687. https://doi.org/10.3390/ijerph20031687
Zhao L, Gao Y, Sun J, Wang Y, Wang C, Yu S, Wang Z, Li J, Liu R, Kou W. The Role of Slurry Reflux in a Corn Stalk Continuous Anaerobic Digestion System: Performance and Microbial Community. International Journal of Environmental Research and Public Health. 2023; 20(3):1687. https://doi.org/10.3390/ijerph20031687
Chicago/Turabian StyleZhao, Ling, Yang Gao, Jiaxing Sun, Yanan Wang, Congxin Wang, Shuai Yu, Zhen Wang, Jingyang Li, Ronghou Liu, and Wei Kou. 2023. "The Role of Slurry Reflux in a Corn Stalk Continuous Anaerobic Digestion System: Performance and Microbial Community" International Journal of Environmental Research and Public Health 20, no. 3: 1687. https://doi.org/10.3390/ijerph20031687