Liquid-Diet with Alcohol Alters Maternal, Fetal and Placental Weights and the Expression of Molecules Involved in Integrin Signaling in the Fetal Cerebral Cortex
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Compliance
2.2. Animals and Alcohol Exposure
2.3. Maternal Weights and Blood Alcohol Concentrations
2.4. Fetal and Placental Weights, and Gross Morphology
2.5. Isolation of Fetal Cerebral Cortices and Western Blotting
2.6. Statistical Analysis
3. Results
3.1. Diet Consumption
3.2. Blood Alcohol Concentration
3.3. Alcohol Effects on Weights of Mothers, Fetuses and Placentas
3.4. Litter Size and Morphology
3.5. Expression Levels of Molecules in the Fetal Cerebral Cortex
4. Discussion
5. Conclusions
Acknowledgments
References
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Antigen Primary | Antibody | Source |
---|---|---|
β-Actin | Mouse monoclonal (Clone C4) | BD Biosciences, San Jose, CA, USA |
GPDH | Mouse monoclonal (Clone 6C5) | Millipore corporation, Billerica, MA, USA |
Integrin subunit β1 | Mouse monoclonal (Clone 18) | BD Biosciences, San Jose, CA, USA |
Integrin subunit α3 | Mouse monoclonal (Clone 42/CD49c) | BD Biosciences, San Jose, CA, USA |
Integrin subunit α6 | Rabbit polyclonal (Purified) | ABGENT, SanDiego, CA, USA |
Phosphoinositide-specific phospholipaseCγ1 (PLCγ1) | Rabbit polyclonal (Purified) | Cell Signaling Technologies, Inc., Danvers, MA, USA |
Phoshpho PLCγ1 (Tyr 783) | Rabbit polyclonal (Purified) | Cell Signaling Technologies, Inc., Danvers, MA, USA |
Phosphoinositide-specific phospholipaseC γ2 (PLCγ2) | Rabbit polyclonal (Q-20) (Purified) | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
N-cadherin | Mouse monoclonal (Clone 13A9) | BD Biosciences, San Jose, CA, USA |
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Rout, U.K.; Dhossche, J.M. Liquid-Diet with Alcohol Alters Maternal, Fetal and Placental Weights and the Expression of Molecules Involved in Integrin Signaling in the Fetal Cerebral Cortex. Int. J. Environ. Res. Public Health 2010, 7, 4023-4036. https://doi.org/10.3390/ijerph7114023
Rout UK, Dhossche JM. Liquid-Diet with Alcohol Alters Maternal, Fetal and Placental Weights and the Expression of Molecules Involved in Integrin Signaling in the Fetal Cerebral Cortex. International Journal of Environmental Research and Public Health. 2010; 7(11):4023-4036. https://doi.org/10.3390/ijerph7114023
Chicago/Turabian StyleRout, Ujjwal K., and Julie M. Dhossche. 2010. "Liquid-Diet with Alcohol Alters Maternal, Fetal and Placental Weights and the Expression of Molecules Involved in Integrin Signaling in the Fetal Cerebral Cortex" International Journal of Environmental Research and Public Health 7, no. 11: 4023-4036. https://doi.org/10.3390/ijerph7114023