In Vitro DNA-Binding, Anti-Oxidant and Anticancer Activity of Indole-2-Carboxylic Acid Dinuclear Copper(II) Complexes
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of ICA-Cu Complexes
2.1.1. Infrared Spectra
2.1.2. UV-Vis Absorption Spectra
2.1.3. 1H-NMR Spectra
2.1.4. Thermogravimetric Analysis
2.1.5. Elemental Analysis
2.1.6. Molar Conductivity
2.2. DNA Binding Studies
2.2.1. Fluorescence Spectroscopy Studies
2.2.2. Studies of Competitive DNA-Binding Ability with EB
2.2.3. Effect of Ionic Strength
2.2.4. Quenching Experiment of [Fe(CN)6]3−
2.2.5. Viscosity Measurement
2.3. Antioxidant Activity
2.4. In Vitro Cytotoxicity Studies
3. Experimental Section
3.1. Materials and Methods
3.2. Synthesis of the Complexes
3.3. DNA Binding Experiments
3.4. Antioxidant Experiments
3.5. In Vitro Anticancer Activity Experiments
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ligand/Complex | ν-NH- | νas(COO−) | νs(COO−) | νCu-O |
---|---|---|---|---|
ICA | 3355.75 | 1696.19 | 1200.91 | - |
ICA-Cu | 3437.61 | 1510.92 | 1344.34 | 484.64 |
Ligand/Complex | λmax |
---|---|
ICA | 292 |
ICA-Cu | 298 |
Ligand/Complex | H-3 | H-4 | H-5 | H-6 | H-7 | -COOH | -NH- |
---|---|---|---|---|---|---|---|
ICA | 7.252 (m) | 7.649 (m) | 7.051 (m) | 7.238 (m) | 7.442 (m) | 11.753 (s) | 12.943 (s) |
ICA-Cu | 7.229 (m) | 7.519 (m) | 6.082 (m) | 7.042 (m) | 7.388 (m) | - | 14.476 (s) |
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Wang, X.; Yan, M.; Wang, Q.; Wang, H.; Wang, Z.; Zhao, J.; Li, J.; Zhang, Z. In Vitro DNA-Binding, Anti-Oxidant and Anticancer Activity of Indole-2-Carboxylic Acid Dinuclear Copper(II) Complexes. Molecules 2017, 22, 171. https://doi.org/10.3390/molecules22010171
Wang X, Yan M, Wang Q, Wang H, Wang Z, Zhao J, Li J, Zhang Z. In Vitro DNA-Binding, Anti-Oxidant and Anticancer Activity of Indole-2-Carboxylic Acid Dinuclear Copper(II) Complexes. Molecules. 2017; 22(1):171. https://doi.org/10.3390/molecules22010171
Chicago/Turabian StyleWang, Xiangcong, Maocai Yan, Qibao Wang, Huannan Wang, Zhengyang Wang, Jiayi Zhao, Jing Li, and Zhen Zhang. 2017. "In Vitro DNA-Binding, Anti-Oxidant and Anticancer Activity of Indole-2-Carboxylic Acid Dinuclear Copper(II) Complexes" Molecules 22, no. 1: 171. https://doi.org/10.3390/molecules22010171
APA StyleWang, X., Yan, M., Wang, Q., Wang, H., Wang, Z., Zhao, J., Li, J., & Zhang, Z. (2017). In Vitro DNA-Binding, Anti-Oxidant and Anticancer Activity of Indole-2-Carboxylic Acid Dinuclear Copper(II) Complexes. Molecules, 22(1), 171. https://doi.org/10.3390/molecules22010171