Novel Self-Assembly-Induced Gelation for Nanofibrous Collagen/Hydroxyapatite Composite Microspheres
Abstract
:1. Introduction
2. Materials and Methods
2.1. Starting Materials
2.2. Collagen/HA Composite Solutions Preparation
2.3. Self-Assembly-Induced Gelation
2.4. Cross-Linking and Freeze Drying
2.5. Morphology, Microstructure, and Nanostructure Characterization
2.6. Chemical Composition and Crystalline Phase Analyses
2.7. TGA Analysis
2.8. In Vitro Apatite-Forming Ability Evaluation
2.9. Bioactive Molecule-Loaded Microsphere Synthesis and Evaluation
3. Results and Discussion
3.1. Morphology of Collagen/HA Composite Microspheres
3.2. Nanofibrous Structure of Collagen/HA Composite Microspheres
3.3. Incorporation of HA Particles into Composite Microspheres
3.4. In Vitro Apatite-Forming Ability of Composite Microspheres
3.5. Utility of Self-Assembly-Induced Gelation
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ion | Na+ | K+ | Mg2+ | Ca2+ | Cl− | HCO3− | HPO42− | SO42− |
---|---|---|---|---|---|---|---|---|
Concentrations (mM) | 142.0 | 5.0 | 1.5 | 2.5 | 148.8 | 4.2 | 1.0 | 0 |
HA Content [wt %] | 0 | 10 | 15 | 20 |
---|---|---|---|---|
Diameter [µm] | 802 ± 40 | 798 ± 42 | 806 ± 38 | 809 ± 34 |
Initial HA Content [wt %] | 10 | 15 | 20 |
Final HA Content [wt %] | 10.4 | 13.3 | 17.6 |
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Choi, J.-W.; Kim, J.-W.; Jo, I.-H.; Koh, Y.-H.; Kim, H.-E. Novel Self-Assembly-Induced Gelation for Nanofibrous Collagen/Hydroxyapatite Composite Microspheres. Materials 2017, 10, 1110. https://doi.org/10.3390/ma10101110
Choi J-W, Kim J-W, Jo I-H, Koh Y-H, Kim H-E. Novel Self-Assembly-Induced Gelation for Nanofibrous Collagen/Hydroxyapatite Composite Microspheres. Materials. 2017; 10(10):1110. https://doi.org/10.3390/ma10101110
Chicago/Turabian StyleChoi, Jae-Won, Jong-Woo Kim, In-Hwan Jo, Young-Hag Koh, and Hyoun-Ee Kim. 2017. "Novel Self-Assembly-Induced Gelation for Nanofibrous Collagen/Hydroxyapatite Composite Microspheres" Materials 10, no. 10: 1110. https://doi.org/10.3390/ma10101110