Next Article in Journal
Alloying and Properties of C14–NbCr2 and A15–Nb3X (X = Al, Ge, Si, Sn) in Nb–Silicide-Based Alloys
Next Article in Special Issue
Effect of Antibacterial Plant Extracts on the Morphology of Electrospun Poly(Lactic Acid) Fibres
Previous Article in Journal
Electrospun Composite Liquid Crystal Elastomer Fibers
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Materials
Volume 11
Issue 3
10.3390/ma11030394
materials-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Protein-Based Nanoparticle Preparation via Nanoprecipitation Method

by
Mohamad Tarhini
1,2,
Ihsane Benlyamani
1,
Selim Hamdani
1,
Géraldine Agusti
1,
Hatem Fessi
1,
Hélène Greige-Gerges
2,
Abderrazzak Bentaher
3 and
Abdelhamid Elaissari
1,*
1
Univ Lyon, Université Claude Bernard Lyon-1, CNRS, LAGEP UMR 5007, 43 boulevard du 11 Novembre 1918, F-69100 Villeurbanne, France
2
Faculty of Sciences, Lebanese University, B.P. 90656 Jdaidet El-Matn, Lebanon
3
Inflammation and Immunity of the Respiratory Epithelium-EA 7426, Faculté de Médecine Lyon Sud, 69495 Pierre Benite, France
*
Author to whom correspondence should be addressed.
Materials 2018, 11(3), 394; https://doi.org/10.3390/ma11030394
Submission received: 31 January 2018 / Revised: 1 March 2018 / Accepted: 4 March 2018 / Published: 7 March 2018
(This article belongs to the Special Issue Biodegradable Polymeric Composites: Development and Industrial Applications)
Browse Figures
Versions Notes

Abstract

Nanoparticles are nowadays largely investigated in the field of drug delivery. Among nanoparticles, protein-based particles are of paramount importance since they are natural, biodegradable, biocompatible, and nontoxic. There are several methods to prepare proteins containing nanoparticles, but only a few studies have been dedicated to the preparation of protein- based nanoparticles. Then, the aim of this work was to report on the preparation of bovine serum albumin (BSA)-based nanoparticles using a well-defined nanoprecipitation process. Special attention has been dedicated to a systematic study in order to understand separately the effect of each operating parameter of the method (such as protein concentration, solvent/non-solvent volume ratio, non-solvent injection rate, ionic strength of the buffer solution, pH, and cross-linking) on the colloidal properties of the obtained nanoparticles. In addition, the mixing processes (batch or drop-wise) were also investigated. Using a well-defined formulation, submicron protein-based nanoparticles have been obtained. All prepared particles have been characterized in terms of size, size distribution, morphology, and electrokinetic properties. In addition, the stability of nanoparticles was investigated using Ultraviolet (UV) scan and electrophoresis, and the optimal conditions for preparing BSA nanoparticles by the nanoprecipitation method were concluded.
Keywords: bovine serum albumin; colloids; nanoparticles; nanoprecipitation; size bovine serum albumin; colloids; nanoparticles; nanoprecipitation; size
Graphical Abstract

Share and Cite

MDPI and ACS Style

Tarhini, M.; Benlyamani, I.; Hamdani, S.; Agusti, G.; Fessi, H.; Greige-Gerges, H.; Bentaher, A.; Elaissari, A. Protein-Based Nanoparticle Preparation via Nanoprecipitation Method. Materials 2018, 11, 394. https://doi.org/10.3390/ma11030394

AMA Style

Tarhini M, Benlyamani I, Hamdani S, Agusti G, Fessi H, Greige-Gerges H, Bentaher A, Elaissari A. Protein-Based Nanoparticle Preparation via Nanoprecipitation Method. Materials. 2018; 11(3):394. https://doi.org/10.3390/ma11030394

Chicago/Turabian Style

Tarhini, Mohamad, Ihsane Benlyamani, Selim Hamdani, Géraldine Agusti, Hatem Fessi, Hélène Greige-Gerges, Abderrazzak Bentaher, and Abdelhamid Elaissari. 2018. "Protein-Based Nanoparticle Preparation via Nanoprecipitation Method" Materials 11, no. 3: 394. https://doi.org/10.3390/ma11030394

APA Style

Tarhini, M., Benlyamani, I., Hamdani, S., Agusti, G., Fessi, H., Greige-Gerges, H., Bentaher, A., & Elaissari, A. (2018). Protein-Based Nanoparticle Preparation via Nanoprecipitation Method. Materials, 11(3), 394. https://doi.org/10.3390/ma11030394

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop