Pullulan/Poly(Vinyl Alcohol) Composite Hydrogels for Adipose Tissue Engineering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Preparation of HP/PVA Crosslinked Composite Hydrogels
2.2.2. FTIR Spectroscopy
2.2.3. Morphology
2.2.4. Swelling and Dissolution Behavior
2.2.5. In Vitro Degradation Studies
2.2.6. Biocompatibility Studies
2.2.7. Adipogenic Differentiation Assessment
2.2.8. Statistical Analysis
3. Results and Discussion
3.1. Preparation and Characterization of HP/PVA Composite Hydrogels
3.1.1. Morphology
3.1.2. Chemical Structure
3.2. Swelling and Dissolution Behavior
3.3. In Vitro Degradation Studies
3.4. Biocompatibility
3.5. Adipogenic Differentiation Assessment
3.5.1. Adipogenic Marker Perilipin Gene Expression Analysis
3.5.2. Adipogenic Marker Perilipin Protein Expression
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Code | Composition P/PVA (g %) | Crosslinking Method | Phosphate Groups Content * (mmol/g Hydrogel) | |
---|---|---|---|---|
Procedure | Method | |||
HP/PVA47-R (average Mw ~ 47,000 g/mol, crosslinked at room temp) | 50/50 | Room temperature | chemical | 1.93 ± 0.15 |
HP/PVA47-F (average Mw ~ 47,000 g/mol, crosslinked by cryogelation) | 50/50 | Cryogelation | Chemical and physical | 1.39 ± 0.24 |
HP/PVA125-F (average Mw ~ 125,000 g/mol, crosslinked by cryogelation) | 75/25 | Cryogelation | Chemical and physical | 1.73 ± 0.26 |
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Samoila, I.; Dinescu, S.; Pircalabioru, G.G.; Marutescu, L.; Fundueanu, G.; Aflori, M.; Constantin, M. Pullulan/Poly(Vinyl Alcohol) Composite Hydrogels for Adipose Tissue Engineering. Materials 2019, 12, 3220. https://doi.org/10.3390/ma12193220
Samoila I, Dinescu S, Pircalabioru GG, Marutescu L, Fundueanu G, Aflori M, Constantin M. Pullulan/Poly(Vinyl Alcohol) Composite Hydrogels for Adipose Tissue Engineering. Materials. 2019; 12(19):3220. https://doi.org/10.3390/ma12193220
Chicago/Turabian StyleSamoila, Iuliana, Sorina Dinescu, Gratiela Gradisteanu Pircalabioru, Luminita Marutescu, Gheorghe Fundueanu, Magdalena Aflori, and Marieta Constantin. 2019. "Pullulan/Poly(Vinyl Alcohol) Composite Hydrogels for Adipose Tissue Engineering" Materials 12, no. 19: 3220. https://doi.org/10.3390/ma12193220