Development of a 3-D Physical Dynamics Monitoring System Using OCM with DVC for Quantification of Sprouting Endothelial Cells Interacting with a Collagen Matrix
Abstract
:1. Introduction
2. Materials and Methods
2.1. Imaging System
2.1.1. Gabor-Domain Optical Coherence Microscope
2.1.2. Second-Harmonic Generation Microscope
2.2. Image Acquisition Process
2.2.1. SHGM Image Acquisition
2.2.2. OCM Image Acquisition and Postprocessing
2.3. Sample Preparation
2.3.1. Microfluidic Chip Fabrication
2.3.2. Collagen Type I Gel Preparation
2.3.3. Generating Strain with Nd Magnet and Steel Rod
2.3.4. HUVEC Preparation and Imaging
2.4. DVC Analysis
2.4.1. Error Floor Evaluation
2.4.2. Drift Correction and Calculation of the Incremental Displacement
2.4.3. Strain Calculation
3. Results
3.1. GD-OCM System Configuration and Performance Verification
3.2. Optimization of the Efficient Sampling Rate for Detecting Collagen Gel Structural Deformation
3.3. Quantification and Visualization of Collagen Gel Deformation and Its Strain
3.4. Observation of the ECM-Mediated Physical Dynamics of Sprouting Vascular Endothelial Cell and Distant Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Type | Error Floor STD 1 (IQR 2) | Displacement Mean (Median) | |||||
---|---|---|---|---|---|---|---|
Sampling Rate (µm/px) | 0.8 | 0.53 | 0.4 | 0.8 | 0.53 | 0.4 | GT3 |
U (µm) | 0.111 | 0.047 | 0.103 | 3.195 | 3.199 | 3.199 | 3.20 |
(0.147) | (0.071) | (0.160) | (3.198) | (3.200) | (3.199) | ||
V (µm) | 0.068 | 0.040 | 0.208 | 3.195 | 3.199 | 3.199 | 3.20 |
(0.094) | (0.060) | (0.377) | (3.198) | (3.199) | (3.199) | ||
W (µm) | 0.150 | 0.162 | 0.201 | 4.417 | 4.423 | 4.417 | 4.42 |
(0.220) | (0.209) | (0.313) | (4.422) | (4.424) | (4.419) | ||
Average (µm) | 0.110 | 0.083 | 0.171 | 3.602 | 3.607 | 3.605 | 3.61 |
(0.154) | (0.113) | (0.283) | (3.606) | (3.608) | (3.606) |
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Kang, Y.G.; Jang, H.; Park, Y.; Kim, B.-M. Development of a 3-D Physical Dynamics Monitoring System Using OCM with DVC for Quantification of Sprouting Endothelial Cells Interacting with a Collagen Matrix. Materials 2020, 13, 2693. https://doi.org/10.3390/ma13122693
Kang YG, Jang H, Park Y, Kim B-M. Development of a 3-D Physical Dynamics Monitoring System Using OCM with DVC for Quantification of Sprouting Endothelial Cells Interacting with a Collagen Matrix. Materials. 2020; 13(12):2693. https://doi.org/10.3390/ma13122693
Chicago/Turabian StyleKang, Yong Guk, Hwanseok Jang, Yongdoo Park, and Beop-Min Kim. 2020. "Development of a 3-D Physical Dynamics Monitoring System Using OCM with DVC for Quantification of Sprouting Endothelial Cells Interacting with a Collagen Matrix" Materials 13, no. 12: 2693. https://doi.org/10.3390/ma13122693