Optimizing Silanization to Functionalize Stainless Steel Wire: Towards Breast Cancer Stem Cell Isolation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Pre-Treatment of SS
2.2. Electrodeposition of APTES
2.3. Cyclic Voltammetry
2.4. Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Spectroscopy (EDS) Analysis
2.5. FITC Analysis
2.6. Immobilizing Ligands
2.7. Atomic Force Microscopy (AFM)
2.8. Testing the Functionalized SS Surface to Capture BCSC
3. Results and Discussion
3.1. Pre-Treatment of SS
3.2. Electrodeposition of APTES: General Consideration
3.3. Electrodeposition of APTES: Applied Potential
3.4. Electrodeposition of APTES: pH of the Solution
3.5. Electrodeposition of APTES: Heat Treatment Temperature
3.6. Attaching Aptamers
3.7. Testing the Surface to Capture Cells
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Bekmurzayeva, A.; Dukenbayev, K.; Azevedo, H.S.; Marsili, E.; Tosi, D.; Kanayeva, D. Optimizing Silanization to Functionalize Stainless Steel Wire: Towards Breast Cancer Stem Cell Isolation. Materials 2020, 13, 3693. https://doi.org/10.3390/ma13173693
Bekmurzayeva A, Dukenbayev K, Azevedo HS, Marsili E, Tosi D, Kanayeva D. Optimizing Silanization to Functionalize Stainless Steel Wire: Towards Breast Cancer Stem Cell Isolation. Materials. 2020; 13(17):3693. https://doi.org/10.3390/ma13173693
Chicago/Turabian StyleBekmurzayeva, Aliya, Kanat Dukenbayev, Helena S. Azevedo, Enrico Marsili, Daniele Tosi, and Damira Kanayeva. 2020. "Optimizing Silanization to Functionalize Stainless Steel Wire: Towards Breast Cancer Stem Cell Isolation" Materials 13, no. 17: 3693. https://doi.org/10.3390/ma13173693