pH-Responsive and Biodegradable ZnO-Capped Mesoporous Silica Composite Nanoparticles for Drug Delivery
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of ZnO QDs
2.3. Synthesis and Functionalization of MSCNs
2.3.1. Synthesis of MSCNs
2.3.2. Amino Functionalization of MSCNs
2.3.3. Carboxyl Functionalization of MSCNs
2.4. Biodegradation of MSCNs in PBS Solution and in Cells
2.5. ZnO Capped DOX@MSCNs
2.5.1. Synthesis of DOX@MSCNs
2.5.2. ZnO-Capped DOX@MSCNs
2.6. In Vitro pH-Responsive Drug Release of DOX@MSCNs-ZnO
2.7. Cell Culture
2.8. Intracellular Endocytosis of DOX@MSCNs-ZnO
2.9. In Vitro Cytotoxicity Assays
2.10. Characterization of Nanoparticles
2.11. Statistical Analysis
3. Results and Discussions
3.1. Synthesis and Characterization of ZnO QDs
3.1.1. Morphology and Particle Size of ZnO QDs
3.1.2. Elemental and Structural Analysis of ZnO QDs
3.1.3. Acidic Degradation Behavior of ZnO QDs
3.2. Synthesis and Characterization of MSCNs
3.2.1. Morphology and Particle Size of MSCNs
3.2.2. Structure, Specific Surface Area and Pore Diameter of MSCNs
3.2.3. Elemental Analysis of MSCNs
3.3. The Biodegradation Behavior of MSCNs
3.3.1. Degradation of MSCNs in PBS Solution
3.3.2. Degradation of MSCNs in 4T1 Cells
3.4. ZnO-Capped DOX@MSCNs
3.5. In Vitro pH-Responsive Drug Release of DOX@MSCNs-ZnO
3.6. Endocytosis of DOX@MSCNs-ZnO
3.7. In Vitro Cell Cytotoxicity
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Chen, M.; Hu, J.; Bian, C.; Zhu, C.; Chen, C.; Guo, Z.; Zhang, Z.; Agyekum, G.A.; Zhang, Z.; Cao, X. pH-Responsive and Biodegradable ZnO-Capped Mesoporous Silica Composite Nanoparticles for Drug Delivery. Materials 2020, 13, 3950. https://doi.org/10.3390/ma13183950
Chen M, Hu J, Bian C, Zhu C, Chen C, Guo Z, Zhang Z, Agyekum GA, Zhang Z, Cao X. pH-Responsive and Biodegradable ZnO-Capped Mesoporous Silica Composite Nanoparticles for Drug Delivery. Materials. 2020; 13(18):3950. https://doi.org/10.3390/ma13183950
Chicago/Turabian StyleChen, Minmin, Jinxia Hu, Cancan Bian, Chenghao Zhu, Chen Chen, Zhijun Guo, Zhimin Zhang, Godfred Amfo Agyekum, Zhuoqi Zhang, and Xichuan Cao. 2020. "pH-Responsive and Biodegradable ZnO-Capped Mesoporous Silica Composite Nanoparticles for Drug Delivery" Materials 13, no. 18: 3950. https://doi.org/10.3390/ma13183950