Next Article in Journal
Evaluation of Ti–Mn Alloys for Additive Manufacturing Using High-Throughput Experimental Assays and Gaussian Process Regression
Previous Article in Journal
Parametric Formula for Stress Concentration Factor of Fillet Weld Joints with Spline Bead Profile
Previous Article in Special Issue
Assessment of the Impact of the Addition of Nanoparticles on the Properties of Glass–Ionomer Cements
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Anti-biofilm Fe3O4@C18-[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate Derivative Core-shell Nanocoatings

by
Rodica Olar
1,*,
Mihaela Badea
1,
Cătălin Maxim
1,
Alexandru Mihai Grumezescu
2,
Coralia Bleotu
3,
Luminiţa Măruţescu
4,5 and
Mariana Carmen Chifiriuc
4,5,6,*
1
Department of Inorganic Chemistry, Faculty of Chemistry, University of Bucharest, 90–92 Panduri Str., 050663 Bucharest, Romania
2
Department of Science and Engineering of Oxidic Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
3
Stefan S Nicolau Institute of Virology, Romanian Academy, 285 Mihai Bravu Ave., 030304 Bucharest, Romania
4
Department of Microbiology, Faculty of Biology, University of Bucharest, 1–3 Aleea Portocalelor St., 60101 Bucharest, Romania
5
Environment and Earth Sciences Department, Research Institute of the University of Bucharest—ICUB, Splaiul Independenţei 91–95, 050095 Bucharest, Romania
6
Academy of Romanian Scientists, 010071 Bucharest, Romania
*
Authors to whom correspondence should be addressed.
Materials 2020, 13(20), 4640; https://doi.org/10.3390/ma13204640
Submission received: 7 September 2020 / Revised: 7 October 2020 / Accepted: 13 October 2020 / Published: 17 October 2020

Abstract

:
The derivatives 5,7-dimethyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (1) and 7-methyl-5-phenyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (2) were fully characterized by single-crystal X-ray diffraction. Their supramolecular structure is built through both π–π stacking and C=S–π interactions for both compounds. The embedment of the tested compounds into Fe3O4@C18 core-shell nanocoatings increased the protection degree against Candida albicans biofilms on the catheter surface, suggesting that these bioactive nanocoatings could be further developed as non-cytotoxic strategies for fighting biofilm-associated fungal infections.

Graphical Abstract

1. Introduction

Despite significant research progress in developing novel anti-infectious therapeutic and preventive strategies, infectious diseases treatment still remains a big challenge, primarily because of the emergence of microbial resistance to nearly all antibiotics used clinically [1,2,3]. The most threatening are those produced by the multiple drug-resistant (MDR) bugs that emerged in both hospitals and the community. The Gram-negative MDR bugs are intrinsically or clinically resistant to all currently available antibiotics (mediated by efflux pumps overexpression or by the presence of protective outer membrane), the resistance genes being often located on mobile genetic elements, facilitating their accumulation and dissemination [4].
Many efforts are directed towards searching either novel compounds with potent bioactivities or new ways of formulation or administration for achieving efficient tools to fight especially multi-resistant strains or biofilm formation on medical implants [5,6]. Once adhered and protected by a self-secreted extracellular polymeric matrix, microbial cells switch to a modified phenotype, with a different growth rate and transcriptome, becoming up to 1000-fold more tolerant to antibiotics. Usually, the active antibiotic concentrations are determined by standardized antimicrobial susceptibility tests performed on planktonic cells, which are not active against biofilms [7].
One of the most promising leads for new antimicrobial strategies is to design antimicrobials with dual/multiple mechanisms of action, hybrid antibiotics, which are defined as synthetic constructs with >2 pharmacophores with antimicrobial activity or of hybrid materials combining different antimicrobials in a single system [8,9,10]. In this way, the required active dose and the risk of resistance selection are minimized, increasing efficiency and reducing the side effects.
Nanotechnology proposed various nanomaterials to fight resistant bacterial and biofilms, either based on their intrinsic antimicrobial (microbiostatic/microbicidal/antipathogenic) properties or on their potential to be used as delivery systems for antimicrobial drugs [5,9,11,12]. In this regard, the hybrid nanosystems based on magnetite nanoparticles (MGNPs) could represent an alternative, considering their accessible and versatile preparation, reduced toxicity as well as antimicrobial properties [13,14,15,16]. MGNPs were authorized as in vivo drug delivery platform, and as a result, several loaded systems with a good antimicrobial activity were recently reported [17,18,19,20,21,22,23].
On the other hand, a variety of biomolecules have been used in order to facilitate the interaction of MGNPs with other antimicrobial species [20], including fatty acids such as stearic and myristic ones [17,24].
Nitrogen-based heterocycles are most studied as biologically active species, and among these, the 1,3,4-thiadiazole moiety exhibit manifold importance as a potent chemotherapeutic agent, this being the active pharmacophore in several drugs. Thus acetazolamide and methazolamide are used for the treatment of glaucoma, megazol in Tripanosoma brucei and T. cruzi parasitoses, while sulfamethoxazole, cefazolin, and cefazedone are large spectrum antibiotics [25,26,27,28].
Moreover, thiadiazole is a versatile substrate for designing a large diversity of fused heterocycles, such as those with triazole [29,30,31,32,33,34], imidazole [35,36,37,38,39], and pyrimidine [40,41,42,43,44,45,46,47,48,49,50,51,52], most of them with confirmed biological activity.
Among these, the thiadiazolopyrimidine fused systems are particularly interesting as mesoionic purine bases analogs. Otherwise, the [1,3,4]thiadiazolo [3,2-a]pyrimidine core induces a broad spectrum of pharmacological activities, including antimicrobial [40,41,42,43,44,45,46] and antitumoral ones [47,48,49,50,51,52].
Using a nanobiotechnological approach, we have combined the tools and materials of synthetic chemistry, nanoscience, and biology to address the antimicrobial resistance threat. As a result, this paper focuses on the structural characterization and evaluation of the antibiofilm activity of two bioactive nanosystems based on [1,3,4]thiadiazolo [3,2-a]pyrimidinium derivatives and Fe3O4@C18 core@shell nanoparticles against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans strains in order to develop a new strategy for preventing both formation and eradication of microbial biofilms formed on medical devices. This research is based on previous data concerning the antimicrobial activity of MGNPs [13,14,15,16] and synergy observed with antimicrobials [17,18,19,20,53,54], a strategy that could be used in order to develop valuable nanocarriers for a controlled or a targeted release of an active agent. The interaction between the MGNPs and the [1,3,4]thiadiazolo[3,2-a]-pyrimidinium derivatives was facilitated by octadecanoic acid (C18). Furthermore, the crystallographic data concerning 5,7-dimethyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (1) and 7-methyl-5-phenyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (2) are reported together with their cytotoxicity against HeLa cells.

2. Experimental

2.1. General Information

The reagents of high purity grade were purchased from Merck (Darmstadt, Germany, pentane-2,4-dione, 1-phenyl-1,3-butanedione), Fluka (Fluka Chemical-Sigma Aldrich, Taufkirchen, Germany, 5-amino-1,3,4-thiadiazole-2-thiol), and Sigma Aldrich (Munich, Germany, FeCl3 and FeSO4⋅7H2O), and used as received without further purification.
The C, N, and H content was obtained by a Perkin Elmer PE 2400 analyzer (Perkin Elmer, Waltham, MA, USA). FT-IR spectra in KBr pellets were recorded by a Bruker Tensor 37 spectrometer (Bruker, Billerica, MA, USA) (400–4000 cm−1 range). 1H-NMR and 13C-NMR spectra were recorded at 25 °C on a Bruker Avance spectrometer (Bruker, Karlsruhe, Germany) (working frequency 200 MHz). The chemical shifts (parts per million) were related to the tetramethylsilane (TMS) as an internal standard.

2.2. Synthesis and Spectral Data for [1,3,4]thiadiazolo[3,2-a]pyrimidine Derivatives (Adsorption-Shell)

In order to synthesize the compounds, a modified procedure was used [55]. To a solution containing pentane-2,4-dione/1-phenyl-1,3-butanedione (0.02 mol) in ethanol (25 mL), 5-amino-1,3,4-thiadiazole-2-thiol (0.02 mol) in ethanol (25 mL) was added, followed by a few drops of CH3COOH. The reaction mixture was refluxed 12 h when upon a yellow solution was formed. The solution volume was reduced at the half, and the yellow solid product formed was filtered off, washed several times with cold EtOH and ethyl ether. Single crystals suitable for X-ray diffraction were isolated by the slow evaporation of an ethanolic solution.
5,7-Dimethyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (1) Analysis found: C, 42.64; H, 3.49; N, 21.42; S, 32.59, C7H7N3S2 requires: C, 42.62; H, 3.58; N, 21.30; S, 32.51; Yield 93%; IR (KBr pellet, cm−1): υ(CH pym), 3011w; υas(CH3), 2962w; υs(CH3), 2914w; υ(C=N)+υ(C=C), 1602vs, 1522s; δas(CH3), 1418m; δs(CH3), 1367s; TII, 1340vs, TIII, 1053vs; υ(N–N), 957m; TIV 715w; υas(C–S–C), 645w; υs(C–S–C), 535w [56]; 1H-NMR (DMSO-d6) δ (ppm): 2.64 (s, CH3), 2.83 (s, CH3), 7.19 (s, CH pyrimidine); 13C-NMR (DMSO) δ (ppm): 24.5 (CH3), 77.2 (C2 thiadiazole), 117.8 (C6 pyrimidine), 149.9 (C9 pyrimidine), 163.8 (C7 pyrimidine), 165.6 (C5 pyrimidine).
7-Methyl-5-phenyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (2) Analysis found: C, 55.64; H, 3.49; N, 16.32; S, 24.79, C12H9N3S2 requires: C, 55.57; H, 3.50; N, 16.20; S, 24.73; Yield 88%; IR (KBr pellet, cm−1): υ(CH aromatic), 3069w; υ(CH pyrimidine), 3011w; υas(CH3), 2962w; υs(CH3), 2914w; υ(C=N)+υ(C=C), 1600vs, 1570s, 1512s; δas(CH3), 1418m; δs(CH3), 1367s; TII, 1334vs, υ(C–CH), 1266w TIII, 1023vs; υ(N–N), 957m; ρ(CH), 844w, 770w, 660w, TIV 700m; υas(C–S–C), 637w; υs(C–S–C), 546w [56]; 1H-NMR (DMSO-d6) δ (ppm): 2.72 (s, CH3), 7.33 (s, CH pyrimidine), 7.54 (multiplet, Ar-H), 7.93 (d, Ar-H); 13C-NMR (DMSO) δ (ppm): 24.7 (CH3), 77.4 (C2 thiadiazole), 117.3 (C6 pyrimidine), 129.1, 129.4, 130.1, 132.8 (C10–C15; Ar), 149.1 (C9 pyrimidine), 165.2 (C7 pyrimidine), 165.7 (C5 pyrimidine).

2.3. X-ray Crystallography

Crystallographic data were collected with an IPDS II diffractometer (STOE, Darmstadt, Germany) having a Mo-Kα (λ = 0.71073 Å) X-ray tube with a graphite monochromator. A crystal of suitable size was selected from the mother liquor and immersed in paratone oil, then mounted on the tip of glass fiber and cemented using epoxy resin. Data collections: Stoe X-AREA. Cell refinement: Stoe X-AREA [57]. The structures were solved by direct methods and refined with anisotropic displacement parameters based on F2, using SHELXS 97 [58] and SHELXL 97 [59] programs. Packing and H-bonding diagrams are generated by the DIAMOND program. Non-hydrogen atoms were anisotropically refined until convergence was reached. CCDC-1986171 (1) and -1986172 (2) contain the supplementary crystallographic data that can be obtained free of charge from the Cambridge Crystallographic Data Centre via www.ccdc.cam.ac.uk/data_request/cif.

2.4. Synthesis and Characterization of Core@Shell Nanostructure

The core@shell—Fe3O4@C18 nanostructure was obtained as previously reported [17]. Briefly, the octadecanoic acid (C18) was dispersed in 200 mL volume of deionized water, corresponding to a 0.50% (v/w) solution, under vigorous stirring at 60 °C. 5 mL of 25% ammonia solution was added to C18 solution and then 200 mL of deionized water containing 1 g of FeCl3 and 1.6 g of FeSO4⋅7H2O (2:1 molar ratio) were dropwise added, under continuous stirring up to the formation of a black precipitate. The product was washed several times with methanol and deionized water, and separated with a strong NdFeB permanent magnet. Further, the samples were characterized by TEM (FEI Company, Hillsboro, OR, USA), XRD (Shimadzu, Kyoto, Japan) and FTIR (Thermo Nicolet, Madison, WI, USA), as previously reported by the authors [60,61,62].

2.5. The Core@Shell@Adsorption-Shell Nanostructure Deposition on Catheter Samples

The adsorption-shell nanostructure was obtained by grounding 1 mL solution of acetone containing 5 mg of (1) or (2) with 95 mg Fe3O4@C18 up to the complete solvent evaporation (Figure 1). This procedure was three times repeated for a uniform distribution of the samples on the surface of the spherical nanostructure. The catheters were then coated with the suspension of core@shell@adsorption-shell in acetone (0.33% w/v) and sterilized for 15 min by irradiation with ultraviolet light. This procedure was already reported elsewhere [63].

2.6. Screening of the Antibiofilm Activity

Fresh 24 h cultures were prepared on tryptone soya agar (TSA, MS 2000 Trading Impex SRL, Bucharest, Romania) from the microbial strains (Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 90029) (ATCC, Manassas, VA, USA) preserved on glycerol.
Biofilm development in 6-multi well plates (Nunc, Thermo Scientific, Antisel, Bucharest, Romania) was assessed in dynamics. Sterile coated and bare catheter (reference) samples were added in a 6-well plate in 2 mL of sterile saline and inoculated with ~105 colony forming units (CFU)/mL of microbial suspensions. The samples were incubated at 37 °C for different periods of time periods (24, 48, and 72 h, respectively) to assess the temporal dynamics of cultures grown in the presence of coated and uncoated catheter samples. After each incubation time, the samples were taken off from the culture medium, gently washed to remove the non-adherent bacteria, sonicated to detach the adherent bacteria from the catheter surface, and the absorbance of the obtained suspension was measured at 600 nm with an Apollo LB 911ELISA reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany).

2.7. Cytotoxicity Assay

HeLa cells (ATCC CCL-2, Manassas, VA, USA) were used to evaluate the toxicity of compounds. For this, 7.5 × 105 cells/well were seeded in 24 wells plate, in 5% CO2, and humidified atmosphere at 37 °C for 24 h. The treatment using a concentration of compounds between 500 µg/mL and 125 µg/mL was evaluated after 24 h using CellTiter aqueous one solution kit (Promega, Madison, WI, USA), or using MitoTracker Red CMXRos (Thermo Fisher Scientific, Waltham, MA, USA), or was subjected to cell cycle analysis. The cells stained with 50 nM MitoTracker Red CMXRos, a fluorescent dye that stains mitochondria in live cells, were counterstained with 10 µg/mL Hoechst 33342. The stained cells were fixed in 4% paraformaldehyde and photographed using an Observer D1 microscope (Zeiss, Jena, Germany) using 546 nm (red) and 305 nm (blue) filter. For cell cycle analysis, treated and untreated cells were fixed in cold ethanol (70%), stained with 100 µg/mL propidium iodide and analyzed at flow cytometer (Beckman Coulter, Nyon, Switzerland) and analyzed by FlowJo™ Software for Windows, version 7.2.5. (Becton, Dickinson and Company; 2019).

3. Results and Discussion

3.1. Synthesis and Characterization of [1,3,4]thiadiazolo[3,2-a]pyrimidine Derivatives

The reaction in 1:1 molar ratio of acetylacetone/benzoylacetone and 5-amino-1,3,4-thiadiazole-2-thiol produced the species 5,7-dimethyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (1) and 7-methyl-5-phenyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (2) as depicted in Scheme 1.
The IR spectra of the compounds (see the Experimental section) display bands at 1600 and 1510 cm−1 characteristic for thiadiazolopyrimidine and pyrimidine ring vibrations. The bands around 3100, 1550, and 1440 cm−1 correspond to thiadiazole moiety vibrations.
In the 1H-NMR spectrum of 5,7-dimethyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate, the signals at 2.64, and 2.83 ppm are assigned to methyl protons while that at 7.19 arises from the CH group of the pyrimidine ring. For 7-methyl-5-phenyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate, these signals are slightly shifted while the additional ones at 7.54 and 7.93 ppm could be assigned to phenyl ring protons. The 13C-NMR spectra display signals characteristic for pyrimidine moiety around 118, 150, and 166 ppm while the methyl, thiadiazole, and phenyl groups could be identified through signals around 25, 77, and 128–132 ppm, respectively.

3.2. Structural Description of [1,3,4]thiadiazolo[3,2-a]pyrimidine Derivatives

A summary of the crystallographic data and details of data collection of (1) and (2) s given in Table 1, while selected bond lengths and angles are given in Table 2. Compound (1) crystallizes in the monoclinic system, space group P21/n, with one independent molecule in the asymmetric unit (Figure 2).
Several peculiar features were observed when analyzing the bond lengths between the atoms of the pyrimidine ring as well as the thiadiazole C–S and N–N bonds. Thus, the C2–N2 bond length, equal to 1.366(2) Å, is an intermediary value between the length of a C–N bond in an aliphatic chain (usually between 1.472(5)–1.479(5) Å) and in a heterocycle (1.426(12) Å) [65]. The size of the C6–N1 bond of 1.315(2) Å, is smaller than the previous one and corresponds to a double bond length, similar to a distance of azomethine or oxadiazole. The C7–S1 (1.767(2) Å) and C6–S1 (1.7216(18) Å) bonds have representative lengths for simple covalent bonds. As well, an observation was made that the C7-S2 (1.7216(18) Å) bond lengths are slightly higher than a C=S bond length, which confirms the zwitterion model of the compound.
Interestingly, at the supramolecular level, the molecules form supramolecular dimers built by aromatic π–π stacking interactions between pyrimidine rings of two adjacent molecules (distances between centroids 3.557 Å) (Supplementary Figure S1).
Further, these dimers are connected by C=S–π intermolecular interactions between the C(7)=S(2) groups and the pyrimidine rings (Figure 3) with distances of 3.429 Å, resulting in a 2D brick wall supramolecular network. This type of interaction is more common in protein structure chemistry [66,67,68,69,70,71]. The single-crystal X-ray diffraction molecular structure of compound (2) shows the same zwitterion structure with a similar pyrimidine and thiadiazole rings (Figure 4).
Compound (2) crystallizes in the monoclinic system, space group P21/c, with one independent molecule in the asymmetric unit. The thiadiazole-pyrimidine (S1/N3–N2/C2–N1/C6) ring is essentially planar, with a maximum deviation of 0.089 Å for the methyl group. The bond lengths for the two rings are very similar with the first compound (C1–S2=1.684(5) Å, N2–N3=1.378(6) Å, C2–S1=1.761(5) Å, C2–N1=1.314(7) Å, C2–N2=1.374(6) Å). The dihedral angle between the thiadiazole-pyrimidine ring and the benzene ring is 34.691°.
The network stabilization for compound (2) comes from both weak π–π stacking interactions between the thiadiazole (S1/C1–N3/N2–C2) units (a centroid-to-centroid distance of 3.873 Å) and C=S–π intermolecular interactions between the C(1)=S(2) groups and the pyrimidine rings, with distances of 3.592 Å (Figure 5). These columns running along the a axis are packed in the crystal lattice (Supplementary Figure S2) by non-covalent interactions which reinforce the crystal structure cohesion.

3.3. Antimicrobial Activity

The comparative analysis of Figure 6 and Figure 7 reveals that microbial biofilms were generally less developed in the presence of the catheter samples coated with the hybrid nanomaterials, as compared to the uncoated catheter or to the catheter coated with the free compounds, as indicated by the lower absorbance values of the microbial suspensions recovered from the catheter samples at different time points.
The bacterial and fungal biofilms grown in the presence of the uncoated catheter samples followed a different dynamic, recording a plateau in case of the three tested time points in the case of S. aureus, an exponential growth from 24 to 72 h for P. aeruginosa, and a decrease of biofilm mass installed gradually after 24 h for C. albicans.
Regarding the dynamics of microbial biofilms developed on catheter samples functionalized either with the two compounds or with the hybrid nanostructures respectively, although the growth trend line is relatively similar with that of control biofilm, however, a stronger inhibition of biofilm growth is evident in the case of the hybrid nanostructure.
The most significant biofilm inhibition is observed in case of P. aeruginosa biofilm grown on the catheter coated with the hybrid nanomaterials containing both compounds (1) (at all three time points) and (2) (mostly at 72 h). These results are significant, taking into account the high capacity of this opportunistic and nosocomial, multi-drug resistant pathogen to persist in the hospital environment and to produce biofilm-associated infections.

3.4. Cytotoxicity Assay

In order to estimate the cytotoxicity of the compounds, HeLa cells were exposed to the binary dilution of the species for 24 h and evaluated using CellTiter assay. Compound (1) demonstrated only weak toxicity at 500 µg/mL, while compound (2) was slightly more toxic. In the presence of (2), the cells died, and only a few adherent cells were stained with MitoTracker red. At 500 µg/mL of (2), the morphology of the cells was changed and the Hoechst dye staining was more intense, proving that those cells were dead (Figure 8).
The tested species did not exhibit any remarkable effects on the cellular cycle. Only the G2/M phase is slightly diminished at concentrations above 250 µg/mL (Table 3). The apoptosis peak is observed to the left of the G0/G1 phase and is proportional to the compound concentration (Figure 9). The differences in the cytotoxicity of these two compounds can be at least partially explained by the fact that the phenyl radical on the pyrimidine ring confers enhanced lipophilicity to the compound (2), and this can increase its ability to enter into the tumor cells.
It is worth mention that the catheter samples functionalized with the tested compounds did not have any cytotoxic effect on HeLa cells.

4. Conclusions

The species 5,7-dimethyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (1) and 7-methyl-5-phenyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (2) have been fully characterized by single-crystal X-ray diffraction that evidenced a zwitterion structure with π–π stacking and C=S–π interactions between species in solid network. The embedment of the tested compounds into Fe3O4@C18 core-shell nanocoating induced an increased anti-biofilm activity, reducing the colonization of the catheter surface with C. albicans, suggesting that these bioactive systems could be considered a promising biocompatible strategy foe efficiently fighting against biofilm-associated fungal infections.

Supplementary Materials

The following are available online at https://www.mdpi.com/1996-1944/13/20/4640/s1, Figure S1: Supramolecular dimer of (1) in the solid-state, Figure S2: Crystal packing of (2) in the ab plane.

Author Contributions

Conceptualization, R.O., M.B., A.M.G. and M.C.C.; methodology, L.M., A.M.G. and C.B.; formal analysis, R.O., M.B., A.M.G. and M.C.C; investigation, R.O., M.B., C.M., L.M. and A.M.G.; data curation, C.M., M.B., R.O., L.M., and A.M.G.; writing—original draft preparation, R.O., C.M., A.M.G., C.B. and M.C.C.; writing—review and editing, R.O., C.M., A.M.G. and M.C.C.. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by JSPS Grant-in-Aid for Scientific Research (B), grant number 17H03293. MCC acknowledges Romanian National Authority for Scientific Research and Innovation, UEFISCDI, for financial support through research projects PDI-PFE-CDI ID 335 “Interdisciplinary Institutional Platform for Excellence in Research, Development, Innovation and Professional Training in Archaeological Sciences (ArchaeoScience # RO)”, PCCDI/2018 no. 52,,Multidisciplinary and complex platform for the integrative and systematic research of the identity of the tangible and non-tangible cultural heritage of Romania. Subproject 3-New technologies for preservation, conservation, recovering and restoration of the cultural heritage” and CNFIS-FDI-2020-0355.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Datta, P.; Gupta, V. Next-generation strategy for treating drug resistant bacteria: Antibiotic hybrids. Indian, J. Med. Res. 2019, 149, 97–106. [Google Scholar] [CrossRef] [PubMed]
  2. Podgoreanu, P.; Negrea, S.M.; Buia, R.; Delcaru, C.; Trusca, S.B.; Lazar, V.; Chifiriuc, M.C. Alternative strategies for fighting multidrug resistant bacterial infections. Biointerface Res. Appl. Chem. 2019, 9, 3834–3841. [Google Scholar] [CrossRef]
  3. Ducu, R.; Gheorghe, I.; Chifiriuc, M.C.; Mihăescu, G.; Sârbu, I. Prevalence of vancomycin resistance phenotypes among Enterococcus species isolated from clinical samples in a Romanian hospital. Biointerface Res. Appl. Chem. 2019, 9, 4699–4704. [Google Scholar] [CrossRef]
  4. Vrancianu, C.O.; Popa, L.I.; Bleotu, C.; Chifiriuc, M.C. Targeting plasmids to limit acquisition and transmission of antimicrobial resistance. Front. Microbiol. 2020, 11, 761. [Google Scholar] [CrossRef] [PubMed]
  5. Bahramian, G.; Golestan, L.; Khosravi-Darani, K. Antimicrobial and antioxidant effect of nanoliposomes containing zataria multiflora boiss essential oil on the rainbow trout fillets during refrigeration. Biointerface Res. Appl. Chem. 2018, 8, 3505–3513. [Google Scholar]
  6. Dos Santos, L.D.R.; Dos Santos, A.E.S.; Cerávolo, I.P.; Figueiredo, F.J.B.; Dias-Souza, M.V. Antibiofilm activity of black tea leaf extract, its cytotoxicity and interference on the activity of antimicrobial drugs. Biointerface Res. Appl. Chem. 2018, 8, 3565–3569. [Google Scholar]
  7. Kasimanickam, R.; Ranjan, A.; Asokan, G.V.; Kasimanickam, V.R.; Kastelic, J. Prevention and treatment of biofilms by hybrid- and nanotechnologies. Int. J. Nanomed. 2013, 8, 2809–2819. [Google Scholar] [CrossRef] [PubMed]
  8. Domalaon, R.; Idowu, T.; Zhanel, G.G.; Schweizer, F. Antibiotic hybrids: The next generation of agents and adjuvants against gram-negative pathogens? Clin. Microbiol. Rev. 2018, 31, e00077-17. [Google Scholar] [CrossRef] [Green Version]
  9. Khanmohammadi, S.; Karimian, R.; Hajibonabi, F.; Mostafidi, E.; Tanomand, A.; Edjlali, L.; Azami, Z.K.; Mehrabani, M.G.; Kafil, H.S. Polythiophene/TiO2 and polythiophene/ZrO2 nanocomposites: Physical and antimicrobial properties against common infections. Biointerface Res. Appl. Chem. 2018, 8, 3457–3462. [Google Scholar]
  10. Theuretzbacher, U. Dual-mechanism antibiotics. Nat. Microbiol. 2020, 5, 984–985. [Google Scholar] [CrossRef]
  11. Del Olmo, J.A.; Ruiz-Rubio, L.; Pérez-Álvarez, L.; Saez-Martinez, V.; Vilas-Vilela, J.L. Antibacterial coatings for improving the performance of biomaterials. Coatings 2020, 10, 139. [Google Scholar] [CrossRef] [Green Version]
  12. Pircalabioru, G.G.; Chifiriuc, M.C. Nanoparticulate drug-delivery systems for fighting microbial biofilms: From bench to bedside. Futur. Microbiol. 2020, 15, 679–698. [Google Scholar] [CrossRef]
  13. Toledo, L.D.A.S.D.; Rosseto, H.C.; Bruschi, M.L. Iron oxide magnetic nanoparticles as antimicrobials for therapeutics. Pharm. Dev. Technol. 2017, 23, 316–323. [Google Scholar] [CrossRef] [PubMed]
  14. Rodrigues, G.R.; López-Abarrategui, C.; Gómez, I.D.L.S.; Dias, S.C.; Otero-González, A.J.; Franco, O.L. Antimicrobial magnetic nanoparticles based-therapies for controlling infectious diseases. Int. J. Pharm. 2019, 555, 356–367. [Google Scholar] [CrossRef] [PubMed]
  15. Gabrielyan, L.; Hovhannisyan, A.; Gevorgyan, V.; Ananyan, M.; Trchounian, A. Antibacterial effects of iron oxide (Fe3O4) nanoparticles: Distinguishing concentration-dependent effects with different bacterial cells growth and membrane-associated mechanisms. Appl. Microbiol. Biotechnol. 2019, 103, 2773–2782. [Google Scholar] [CrossRef] [PubMed]
  16. Sandhya, J.; Kalaiselvam, S. Biogenic synthesis of magnetic iron oxide nanoparticles using inedible borassus flabellifer seed coat: Characterization, antimicrobial, antioxidant activity and in vitro cytotoxicity analysis. Mater. Res. Express 2020, 7, 015045. [Google Scholar] [CrossRef]
  17. Limban, C.; Missir, A.V.; Caproiu, M.T.; Grumezescu, A.; Chifiriuc, M.-C.; Bleotu, C.; Marutescu, L.G.; Papacocea, M.T.; Nuță, D.C. Novel hybrid formulations based on thiourea derivatives and Core@Shell Fe3O4@C18 nanostructures for the development of antifungal strategies. Nanomaterials 2018, 8, 47. [Google Scholar] [CrossRef] [Green Version]
  18. Xiong, X.; Huang, Y.; Lin, C.; Liu, X.Y.; Lin, Y. Recent advances in nanoparticulate biomimetic catalysts for combating bacteria and biofilms. Nanoscale 2019, 11, 22206–22215. [Google Scholar] [CrossRef]
  19. Shi, Z.; Jin, L.; He, C.; Li, Y.; Jiang, C.; Wang, H.; Zhang, J.; Wang, J.; He, C.; Zhao, C. Hemocompatible magnetic particles with broad-spectrum bacteria capture capability for blood purification. J. Colloid Interface Sci. 2020, 576, 1–9. [Google Scholar] [CrossRef]
  20. Armijo, L.M.; Wawrzyniec, S.J.; Kopciuch, M.; Brandt, Y.I.; Rivera, A.C.; Withers, N.J.; Cook, N.C.; Huber, D.L.; Monson, T.C.; Smyth, H.D.; et al. Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms. J. Nanobiotechnology 2020, 18, 1–27. [Google Scholar] [CrossRef] [Green Version]
  21. Andersen, M.J.; Flores-Mireles, A.L. Urinary catheter coating modifications: The Race against catheter-associated infections. Coatings 2019, 10, 23. [Google Scholar] [CrossRef] [Green Version]
  22. Patrinoiu, G.; Calderon-Moreno, J.M.; Chifiriuc, M.C.; Saviuc, C.; Birjega, R.; Carp, O. Tunable ZnO spheres with high anti-biofilm and antibacterial activity via a simple green hydrothermal route. J. Colloid Interf. Sci. 2016, 462, 64–74. [Google Scholar] [CrossRef] [PubMed]
  23. Rumyantceva, V.; Rumyantceva, V.; Koshel, E.I.; Vinogradov, V.V. Biocide-conjugated magnetite nanoparticles as an advanced platform for biofilm treatment. Ther. Deliv. 2019, 10, 241–250. [Google Scholar] [CrossRef] [PubMed]
  24. Bilcu, M.; Grumezescu, A.; Grumezescu, A.; Popescu, R.C.; Mogosanu, G.D.; Hristu, R.; Stanciu, S.G.; Mihăilescu, D.F.; Lazar, V.; Bezirtzoglou, E.; et al. Efficiency of vanilla, patchouli and ylang ylang essential oils stabilized by iron oxide@C14 nanostructures against bacterial adherence and biofilms formed by Staphylococcus aureus and Klebsiella pneumoniae clinical strains. Molecules 2014, 19, 17943–17956. [Google Scholar] [CrossRef] [Green Version]
  25. Hu, Y.; Li, C.-Y.; Wang, X.-M.; Yang, Y.-H.; Zhu, H.-L. 1,3,4-Thiadiazole: Synthesis, reactions, and applications in medicinal, agricultural, and materials chemistry. Chem. Rev. 2014, 114, 5572–5610. [Google Scholar] [CrossRef]
  26. Kaur, G.; Singh, R. Thiadiazole analogs as potential pharmacological agents: A brief review. Int. J. Pharm. Pharm. Sci. 2014, 6, 35–46. [Google Scholar]
  27. Haider, S.; Alam, M.S.; Hamid, H. 1,3,4-Thiadiazoles: A potent multi targeted pharmacological scaffold. Eur. J. Med. Chem. 2015, 92, 156–177. [Google Scholar] [CrossRef]
  28. Mehta, D.; Taya, P.; Neetu. A review on the various biological activities of thiadiazole. Int. J. Pharm. Pharm. Sci. 2015, 7, 39–47. [Google Scholar]
  29. Husain, A.; Rashid, M.; Shaharyar, M.; Siddiqui, A.A.; Mishra, R. Benzimidazole clubbed with triazolo-thiadiazoles and triazolo-thiadiazines: New anticancer agents. Eur. J. Med. Chem. 2013, 62, 785–798. [Google Scholar] [CrossRef]
  30. Ramaprasad, G.C.; Kalluraya, B.; Kumar, B.S. Microwave-assisted synthesis of triazolothiadiazole analogs as anticancer agents. Med. Chem. Res. 2014, 23, 3644–3651. [Google Scholar] [CrossRef]
  31. Zhang, L.; Zhao, J.; Zhang, B.; Lu, T.; Chen, Y. Discovery of [1,2,4] triazolo [3,4-b] [1,3,4] thiadiazole derivatives as novel, potent and selective c-Met kinase inhibitors: Synthesis, SAR study, and biological activity. Eur. J. Med. Chem. 2018, 150, 809–816. [Google Scholar] [CrossRef]
  32. Sahu, J.K.; Ganguly, S.; Yasir, M. Synthesis, SAR and molecular docking studies of certain new derivatives of 1,2,4-Triazolo [3,4-b] [1,3,4] thiadiazole as potent antimicrobial agents. Anti-Infect. Agents 2018, 16, 40–48. [Google Scholar] [CrossRef]
  33. Al-Wahaibi, L.H.; Akilandeswari, G.; Anusha, R.; Al-Shaalan, N.H.; Alkmali, O.M.; El-Emam, A.A.; Percino, J.M.; Thamotharan, S. Insights into the nature of weak noncovalent interactions in 3-(4-fluorophenyl)-6-(2-fluorophenyl)-1,2,4-triazolo[3,4-b] [1,3,4] thiadiazole, a potential bioactive agent: X-ray, QTAIM and molecular docking analysis. J. Mol. Struct. 2019, 1183, 331–341. [Google Scholar] [CrossRef]
  34. Settypalli, T.; Rao, C.V.; Kerru, N.; Nallapaneni, H.K.; Chintha, V.R.; Daggupati, T.; Yeguvapalli, S.; Wudayagiri, R. Design, synthesis, neuroprotective and antibacterial activities of 1,2,4-triazolo [3,4-b] 1,3,4-thiadiazole linked thieno [2,3-d] pyrimidine derivatives and in silico docking studies. ChemistySelect 2019, 4, 1627–1634. [Google Scholar] [CrossRef]
  35. Kamal, A.; Ponnampalli, S.; Vishnuvardhan, M.V.P.S.; Rao, M.P.N.; Mullagiri, K.; Nayak, V.L.; Chandrakant, B. Synthesis of imidazothiadiazole–benzimidazole conjugates as mitochondrial apoptosis inducers. MedChemComm 2014, 5, 1644–1650. [Google Scholar] [CrossRef]
  36. Ramprasad, J.; Nayak, N.; Dalimba, U.; Yogeeswari, P.; Sriram, D.; Peethambar, S.; Achur, R.; Dalimba, U. Synthesis and biological evaluation of new imidazo [2,1-b] [1,3,4] thiadiazole-benzimidazole derivatives. Eur. J. Med. Chem. 2015, 95, 49–63. [Google Scholar] [CrossRef]
  37. Cristina, A.; Leonte, D.; Vlase, L.; Bencze, L.C.; Imre, S.; Marc, G.; Apan, B.; Mogosan, C.; Zaharia, V. Heterocycles 48. Synthesis, characterization and biological evaluation of imidazo [2,1-b] [1,3,4] thiadiazole derivatives as anti-inflammatory agents. Molecules 2018, 23, 2425. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  38. Tahtaci, H.; Karacık, H.; Ece, A.; Er, M.; Şeker, M.G. Design, synthesis, SAR and molecular modeling studies of novel imidazo [2,1-b] [1,3,4] thiadiazole derivatives as highly potent antimicrobial agents. Mol. Inform. 2017, 37, 1700083. [Google Scholar] [CrossRef] [PubMed]
  39. Cascioferro, S.; Parrino, B.; Petri, G.L.; Cusimano, M.G.; Schillaci, D.; Di Sarno, V.; Musella, S.; Giovannetti, E.; Cirrincione, G.; Diana, P. 2,6-Disubstituted imidazo[2,1-b] [1,3,4] thiadiazole derivatives as potent staphylococcal biofilm inhibitors. Eur. J. Med. Chem. 2019, 167, 200–210. [Google Scholar] [CrossRef]
  40. Coburn, R.A.; Glennon, R.A. Mesoionic purinone analogs IV: Synthesis and In Vitro antibacterial properties of mesoionic thiazolo [3,2-a] pyrimidin-5,7-diones and mesoionic 1,3,4-thiadiazolo[3,2-a]pyrimidin-5,7-diones. J. Pharm. Sci. 1973, 62, 1785–1789. [Google Scholar] [CrossRef]
  41. Coburn, R.A.; Glennon, R.A.; Chmielewicz, Z.F. Mesoionic purinone analogs. 7. In vitro antibacterial activity of mesoionic 1,3,4-thiadiazolo [3,2-a] pyrimidine-5,7-diones. J. Med. Chem. 1974, 17, 1025–1027. [Google Scholar] [CrossRef] [PubMed]
  42. Mahran, M.A.; El-Obaid, A.M.A.; Badria, F.A. Heterocyclic systems containing pyrimidine nucleus as potential antimicrobial and antitumor agents. Alexandria, J. Pharm. Sci. 1998, 12, 39–44. [Google Scholar]
  43. Deibel, M.R.; Bodnar, A.L.; Yem, A.W.; Wolfe, C.L.; Heckaman, C.L.; Bohanon, M.J.; Mathews, W.R.; Sweeney, M.T.; Zurenko, G.E.; Marotti, K.R.; et al. Immobilization of a novel antibacterial agent on solid phase and subsequent isolation of EF-Tu. Bioconjugate Chem. 2004, 15, 333–343. [Google Scholar] [CrossRef] [PubMed]
  44. El-Gohary, N.S.; Shaaban, M.I. Synthesis, antimicrobial, antiquorum-sensing, antitumor and cytotoxic activities of new series of fused [1,3,4] thiadiazoles. Eur. J. Med. Chem. 2013, 63, 185–195. [Google Scholar] [CrossRef]
  45. El-Gohary, N.S.; Shaaban, M.I. Antimicrobial and antiquorum-sensing studies. Part 2: Synthesis, antimicrobial, antiquorum-sensing and cytotoxic activities of new series of fused [1,3,4] thiadiazole and [1,3] benzothiazole derivatives. Med. Chem. Res. 2013, 23, 287–299. [Google Scholar] [CrossRef]
  46. El-Gohary, N.S.; Shaaban, M.I. Antimicrobial and antiquorum-sensing studies. Part 3: Synthesis and biological evaluation of new series of [1,3,4] thiadiazoles and fused [1,3,4] thiadiazoles. Arch. der Pharm. 2015, 348, 283–297. [Google Scholar] [CrossRef]
  47. El-Ashmawy, M.; El-Sherbeny, M.; El-Sayed, N.S. Synthesis, in vitro antitumor activity and DNA-binding affinity of novel thiadiazolopyrimidine and thiadiazoloquinazoline derivatives. Mansoura, J. Pharm. Sci. 2010, 26, 60–68. [Google Scholar]
  48. El-Sayed, N.S.; El-Bendary, E.R.; El-Ashry, S.M.; El-Kerdawy, M.M. Synthesis and antitumor activity of new sulfonamide derivatives of thiadiazolo [3,2-a] pyrimidines. Eur. J. Med. Chem. 2011, 46, 3714–3720. [Google Scholar] [CrossRef]
  49. Taher, A.T.; Georgey, H.H.; El-Subbagh, H.I. Novel 1,3,4-heterodiazole analogues: Synthesis and in-vitro antitumor activity. Eur. J. Med. Chem. 2012, 47, 445–451. [Google Scholar] [CrossRef]
  50. Hamama, W.S.; Gouda, M.A.; Badr, M.H.; Zoorob, H.H. Synthesis of some new fused and binary 1,3,4-thiadiazoles as potential antitumor and antioxidant agents. J. Heterocycl. Chem. 2013, 50, 787–794. [Google Scholar] [CrossRef]
  51. Rahman, D.E.A.; Mohamed, K.O. Synthesis of novel 1,3,4-thiadiazole analogues with expected anticancer activity. Der Pharma Chemica 2014, 6, 323–335. [Google Scholar]
  52. Baburajeev, C.P.; Mohan, C.D.; Rangappa, S.; Mason, D.J.; Fuchs, J.E.; Bender, A.; Barash, U.; Vlodavsky, I.; Basappa; Rangappa, K.S. Identification of novel class of triazolo-thiadiazoles as potent inhibitors of human heparanase and their anticancer activity. BMC Cancer 2017, 17, 235. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Chifiriuc, M.C.; Grumezescu, A.M.; Andronescu, E.; Ficai, A.; Cotar, A.I.; Grumezescu, V.; Bezirtzoglou, E.; Lazar, V.; Radulescu, R. Water dispersible magnetite nanoparticles influence the efficacy of antibiotics against planktonic and biofilm embedded Enterococcus faecalis cells. Anaerobe 2013, 22, 14–19. [Google Scholar] [CrossRef]
  54. Grumezescu, A.; Gestal, M.C.; Holban, A.; Grumezescu, V.; Vasile, B.S.; Mogoantă, L.; Iordache, F.; Bleotu, C.; Mogosanu, G.D. Biocompatible Fe3O4 increases the efficacy of amoxicillin delivery against gram-positive and gram-negative bacteria. Molecules 2014, 19, 5013–5027. [Google Scholar] [CrossRef] [Green Version]
  55. Abdel-Wahab, B.F.; Mohamed, H.A. Synthetic profiles to different thiadiazolopyrimidines. Phosphorus, Sulfur, Silicon Relat. Elem. 2014, 189, 1780–1793. [Google Scholar] [CrossRef]
  56. Olar, R.; Badea, M.; Marinescu, D.; Lazăr, V.; Chifiriuc, C.; Chifiriuc, M.-C. New complexes of Ni (II) and Cu (II) with Schiff bases functionalised with 1,3,4-thiadiazole: Spectral, magnetic, biological and thermal characterisation. J. Therm. Anal. Calorim. 2009, 97, 721–727. [Google Scholar] [CrossRef]
  57. Stoe & Cie, X-Area. 2002. Available online: https://www.stoe.com/product/software-x-area/ (accessed on 14 October 2020).
  58. Sheldrick, G.M.S. A Program. for the Solution of Crystal; Structures; University of Gottingen: Gottingen, Germany, 1997. [Google Scholar]
  59. Sheldrick, G.M.S. A Program. for Crystal Structures Refinement; University of Göttingen: Göttingen, Germany, 1997. [Google Scholar]
  60. Voicu, G.; Andronescu, E.; Grumezescu, A.; Huang, K.-S.; Ficai, A.; Yang, C.-H.; Bleotu, C.; Chifiriuc, M.-C. Antitumor activity of magnetite nanoparticles: Influence of hydrocarbonated chain of saturated aliphatic monocarboxylic acids. Curr. Org. Chem. 2013, 17, 831–840. [Google Scholar] [CrossRef]
  61. Stecoza, C.E.; Caproiu, M.T.; Draghici, C.; Chifiriuc, M.C.; Dracea, N.O. Synthesis, characterization and antimicrobial activity evaluation of some new derivatives of 6,11-dihydrodibenzo[b,e]thiepin 5,5-dioxide. Rev. Chim. 2009, 6, 137–141. [Google Scholar]
  62. Anghel, I.; Grumezescu, A.M.; Holban, A.M.; Ficai, A.; Anghel, A.G.; Chifiriuc, M.C. Biohybrid nanostructured iron oxide nanoparticles and Satureja hortensis to prevent fungal biofilm development. Int. J. Mol. Sci. 2013, 14, 18110–18123. [Google Scholar] [CrossRef]
  63. Grumezescu, A.; Saviuc, C.; Chifiriuc, M.-C.; Hristu, R.; Mihaiescu, D.E.; Balaure, P.; Stanciu, G.; Lazar, V. Inhibitory activity of Fe3O4 Fe3O4/Oleic Acid/Usnic Acid-Core/Shell/Extra-Shell nanofluid on S. aureus biofilm development. IEEE Trans. NanoBioscience 2011, 10, 269–274. [Google Scholar] [CrossRef]
  64. Anghel, I.; Limban, C.; Grumezescu, A.; Anghel, A.G.; Bleotu, C.; Chifiriuc, M.-C. In vitro evaluation of anti-pathogenic surface coating nanofluid, obtained by combining Fe3O4/C12 nanostructures and 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides. Nanoscale Res. Lett. 2012, 7, 513. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  65. Allen, F.H.; Kennard, O.; Watson, D.G.; Crennell, K.M. Cambridge Crystallographic Data Centre. 6. Preparation and computer typesetting of the molecular structures and dimensions bibliographic volumes. J. Chem. Inf. Model. 1982, 22, 129–139. [Google Scholar] [CrossRef]
  66. Valley, C.C.; Cembran, A.; Perlmutter, J.D.; Lewis, A.K.; Labello, N.P.; Gao, J.; Sachs, J.N. The methionine-aromatic motif plays a unique role in stabilizing protein structure. J. Biol. Chem. 2012, 287, 34979–34991. [Google Scholar] [CrossRef] [Green Version]
  67. Iwaoka, M.; Isozumi, N. Hypervalent nonbonded interactions of a divalent sulfur atom. Implications in protein architecture and the functions. Molecules 2012, 17, 7266–7283. [Google Scholar] [CrossRef] [Green Version]
  68. Reid, K.; Lindley, P.; Thornton, J. Sulphur-aromatic interactions in proteins. FEBS Lett. 1985, 190, 209–213. [Google Scholar] [CrossRef] [Green Version]
  69. Riobé, F.; Avarvari, N. Electroactive oxazoline ligands. Co-ord. Chem. Rev. 2010, 254, 1523–1533. [Google Scholar] [CrossRef]
  70. Lorcy, D.; Bellec, N.; Fourmigué, M.; Avarvari, N. Tetrathiafulvalene-based group XV ligands: Synthesis, coordination chemistry and radical cation salts. Co-ord. Chem. Rev. 2009, 253, 1398–1438. [Google Scholar] [CrossRef]
  71. Pop, F.; Auban-Senzier, P.; Canadell, E.; Rikken, G.L.J.A.; Avarvari, N. Electrical magnetochiral anisotropy in a bulk chiral molecular conductor. Nat. Commun. 2014, 5, 3757. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Figure 1. Schematic illustration of biofilm development in the presence/absence of the coatings [64]. Reprinted from an open access source.
Figure 1. Schematic illustration of biofilm development in the presence/absence of the coatings [64]. Reprinted from an open access source.
Materials 13 04640 g001
Scheme 1. The synthesis procedure for compounds (1) and (2).
Scheme 1. The synthesis procedure for compounds (1) and (2).
Materials 13 04640 sch001
Figure 2. Packing diagram of (1) with π–π and C=S–π interactions.
Figure 2. Packing diagram of (1) with π–π and C=S–π interactions.
Materials 13 04640 g002
Figure 3. The molecular structure with atom numbering for (1).
Figure 3. The molecular structure with atom numbering for (1).
Materials 13 04640 g003
Figure 4. The molecular structure with atom numbering for (2).
Figure 4. The molecular structure with atom numbering for (2).
Materials 13 04640 g004
Figure 5. Supramolecular columns of (2) in the solid-state.
Figure 5. Supramolecular columns of (2) in the solid-state.
Materials 13 04640 g005
Figure 6. Absorbance values of the three tested microbial strains growth dynamics in the presence of uncoated catheter samples and respectively coated with the hybrid nanostructure (1) +N and the free compound (1) −N.
Figure 6. Absorbance values of the three tested microbial strains growth dynamics in the presence of uncoated catheter samples and respectively coated with the hybrid nanostructure (1) +N and the free compound (1) −N.
Materials 13 04640 g006
Figure 7. Absorbance values of the three tested microbial strains growth dynamics in the presence of uncoated catheter samples and respectively coated with the hybrid nanostructure (2) +N and the free compound (2) −N.
Figure 7. Absorbance values of the three tested microbial strains growth dynamics in the presence of uncoated catheter samples and respectively coated with the hybrid nanostructure (2) +N and the free compound (2) −N.
Materials 13 04640 g007
Figure 8. The morphology of Hela cells treated with different concentrations of (1) and (2).
Figure 8. The morphology of Hela cells treated with different concentrations of (1) and (2).
Materials 13 04640 g008aMaterials 13 04640 g008b
Figure 9. Overlapped flow cytometry histograms showing the influence of compounds on the cell cycle phases.
Figure 9. Overlapped flow cytometry histograms showing the influence of compounds on the cell cycle phases.
Materials 13 04640 g009
Table 1. Crystallographic data, details of data collection and structure refinement parameters for (1) and (2).
Table 1. Crystallographic data, details of data collection and structure refinement parameters for (1) and (2).
Compound(1)(2)
Chemical formulaC7H7N3S2C12H9N3S2
M (g mol−1)197.28259.34
Temperature, (K)293293
Wavelength, (Å)0.710730.71073
Crystal systemMonoclinicMonoclinic
Space groupP21/nP21/c
a (Å)7.3084(9)7.4623(11
b (Å)8.4839(11)17.2931(17)
c (Å)14.3377(16)9.8647(13)
α (°)9090
β (°)96.638(9)111.000(10)
γ (°)9090
V3)883.03(19)1188.5(3)
Z44
Dc (g cm-3)1.4841.449
μ (mm−1)0.5470.426
F(000)408536
Goodness-of-fit on F21.0411.079
Final R1, wR2 (I > 2σ(I))0.0399, 0.09230.0714, 0.1998
R1, wR2 (all data)0.0576, 0.09870.0822, 0.2061
Largest diff. peak and hole (eÅ−3)−0.219, 0.223−0.783, 0.722
Table 2. Selected bond lengths (Å) and angles (°) of 1 and 2.
Table 2. Selected bond lengths (Å) and angles (°) of 1 and 2.
Parameter(1)(2)
C1 C21.492(3)C1 N31.337(6)
C2 N11.346(2)C1 S21.684(5)
C2 C31.384(3)C1 S11.761(5)
C3 C41.373(2)C2 N11.314(7)
C3 H30.9300C2 N21.374(6)
C4 N21.364(2)C2 S11.715(5)
C4 C51.479(3)C3 C41.484(8)
C5 H5A0.9600C3 H3A0.9600
C5 H5B0.9600C3 H3B0.9600
C5 H5C0.9600C3 H3C0.9600
C6 N11.315(2)C4 N11.339(7)
C6 N21.366(2)C4 C51.391(8)
C6 S11.7216(18)--
C7 N31.324(2)--
C7 S21.6781(18)--
C7 S11.767(2)--
N2 N31.3786(18)--
N1 C6 S1126.71(15)C7 C12 H12120.5
N2 C6 S1108.88(12)C2 N1 C4116.6(5)
N3 C7 S2125.48(15)C6 N2 C2119.4(4)
N3 C7 S1113.70(13)C6 N2 N3123.0(4)
S2 C7 S1120.82(11)C2 N2 N3117.6(4)
C6 N1 C2116.34(16)C1 N3 N2109.5(4)
C4 N2 C6120.54(15)C2 S1 C190.1(2)
C4 N2 N3121.57(15)C7 C12 H12120.5
C6 N2 N3117.85(14)C2 N1 C4116.6(5)
C7 N3 N2109.71(15)N3 C1 S2125.8(4)
C6 S1 C789.84(9)N3 C1 S1113.6(4)
--S2 C1 S1120.6(3)
Table 3. The influence of compounds on the cell cycle phases.
Table 3. The influence of compounds on the cell cycle phases.
CompoundConcentration (µg/mL)G0/G1 (%)S (%)G2/M (%)
(1)50052.9020.714.52
25055.9224.112.27
12553.7331.189.58
(2)50042.8019.992.52
25052.2221.482.41
12550.5426.439.86
HeLa-56.6130.929.61
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Olar, R.; Badea, M.; Maxim, C.; Grumezescu, A.M.; Bleotu, C.; Măruţescu, L.; Chifiriuc, M.C. Anti-biofilm Fe3O4@C18-[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate Derivative Core-shell Nanocoatings. Materials 2020, 13, 4640. https://doi.org/10.3390/ma13204640

AMA Style

Olar R, Badea M, Maxim C, Grumezescu AM, Bleotu C, Măruţescu L, Chifiriuc MC. Anti-biofilm Fe3O4@C18-[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate Derivative Core-shell Nanocoatings. Materials. 2020; 13(20):4640. https://doi.org/10.3390/ma13204640

Chicago/Turabian Style

Olar, Rodica, Mihaela Badea, Cătălin Maxim, Alexandru Mihai Grumezescu, Coralia Bleotu, Luminiţa Măruţescu, and Mariana Carmen Chifiriuc. 2020. "Anti-biofilm Fe3O4@C18-[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate Derivative Core-shell Nanocoatings" Materials 13, no. 20: 4640. https://doi.org/10.3390/ma13204640

APA Style

Olar, R., Badea, M., Maxim, C., Grumezescu, A. M., Bleotu, C., Măruţescu, L., & Chifiriuc, M. C. (2020). Anti-biofilm Fe3O4@C18-[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate Derivative Core-shell Nanocoatings. Materials, 13(20), 4640. https://doi.org/10.3390/ma13204640

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop