Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice
Abstract
:1. Introduction
2. Results
2.1. Micro-CT and Histological Analysis Following Bone Augmentation with Regular Deproteinized Bovine Bone Mineral (DBBM) Granules and DBBM Fragments
2.2. Particle-Size Distribution and Histological Analysis of Regular DBBM Granules and DBBM Fragments
3. Discussion
4. Material and Methods
4.1. Study Design
4.2. Calvarial Osteolysis Model
4.3. Micro-CT Analysis
4.4. Histological Analysis
4.5. Statistics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Saulacic, N.; Bosshardt, D.D.; Jensen, S.S.; Miron, R.J.; Gruber, R.; Buser, D. Impact of bone graft harvesting techniques on bone formation and graft resorption: A histomorphometric study in the mandibles of minipigs. Clin. Oral Implant. Res. 2014, 26, 383–391. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Gahlert, M.; Schou, S.; Svendsen, P.A.; Forman, J.L.; Gundersen, H.J.G.; Terheyden, H.; Holmstrup, P. Volumetric changes of the graft after maxillary sinus floor augmentation with Bio-Oss and autogenous bone in different ratios: A radiographic study in minipigs. Clin. Oral Implant. Res. 2011, 23, 902–910. [Google Scholar] [CrossRef]
- Busenlechner, D.; Huber, C.; Vasak, C.; Dobsak, A.; Gruber, R.; Watzek, G. Sinus augmentation analysis revised: The gradient of graft consolidation. Bone 2009, 44, S257. [Google Scholar] [CrossRef]
- Cardaropoli, G.; Araújo, M.; Lindhe, J. Dynamics of bone tissue formation in tooth extraction sites. J. Clin. Periodontol. 2003, 30, 809–818. [Google Scholar] [CrossRef]
- Gruber, R. Osteoimmunology: Inflammatory osteolysis and regeneration of the alveolar bone. J. Clin. Periodontol. 2019, 46, 52–69. [Google Scholar] [CrossRef] [Green Version]
- Mbalaviele, G.; Novack, D.V.; Schett, G.; Teitelbaum, S.L. Inflammatory osteolysis: A conspiracy against bone. J. Clin. Investig. 2017, 127, 2030–2039. [Google Scholar] [CrossRef] [Green Version]
- Diarra, D.; Stolina, M.; Polzer, K.; Zwerina, J.; Ominsky, M.S.; Dwyer, D.; Korb, A.; Smolen, J.; Hoffmann, M.; Scheinecker, C.; et al. Dickkopf-1 is a master regulator of joint remodeling. Nat. Med. 2007, 13, 156–163. [Google Scholar] [CrossRef]
- Schett, G.; Neurath, M.F. Resolution of chronic inflammatory disease: Universal and tissue-specific concepts. Nat. Commun. 2018, 9, 1–8. [Google Scholar] [CrossRef]
- Man, K.; Jiang, L.-H.; Foster, R.; Yang, X.B. Immunological Responses to Total Hip Arthroplasty. J. Funct. Biomater. 2017, 8, 33. [Google Scholar] [CrossRef] [Green Version]
- Sun, J.-S.; Liu, H.-C.; Chang, W.H.-S.; Li, J.; Lin, F.-H.; Tai, H.-C. Influence of hydroxyapatite particle size on bone cell activities: Anin vitro study. J. Biomed. Mater. Res. 1998, 39, 390–397. [Google Scholar] [CrossRef]
- Terkawi, M.A.; Hamasaki, M.; Takahashi, D.; Ota, M.; Kadoya, K.; Yutani, T.; Uetsuki, K.; Asano, T.; Irie, T.; Arai, R.; et al. Transcriptional profile of human macrophages stimulated by ultra-high molecular weight polyethylene particulate debris of orthopedic implants uncovers a common gene expression signature of rheumatoid arthritis. Acta Biomater. 2018, 65, 417–425. [Google Scholar] [CrossRef] [PubMed]
- Nich, C.; Takakubo, Y.; Pajarinen, J.; Ainola, M.; Salem, A.; Sillat, T.; Rao, A.J.; Raska, M.; Tamaki, Y.; Takagi, M.; et al. Macrophages-Key cells in the response to wear debris from joint replacements. J. Biomed. Mater. Res. Part A 2013, 101, 3033–3045. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Haugen, H.J.; Lyngstadaas, S.P.; Rossi, F.; Perale, G. Bone grafts: Which is the ideal biomaterial? J. Clin. Periodontol. 2019, 46, 92–102. [Google Scholar] [CrossRef] [PubMed]
- Jensen, S.S.; Bosshardt, D.D.; Gruber, R.; Buser, D. Long-Term Stability of Contour Augmentation in the Esthetic Zone: Histologic and Histomorphometric Evaluation of 12 Human Biopsies 14 to 80 Months After Augmentation. J. Periodontol. 2014, 85, 1549–1556. [Google Scholar] [CrossRef] [PubMed]
- Zecha, P.; Schortinghuis, J.; Van Der Wal, J.; Nagursky, H.; Broek, K.V.D.; Sauerbier, S.; Vissink, A.; Raghoebar, G. Applicability of equine hydroxyapatite collagen (eHAC) bone blocks for lateral augmentation of the alveolar crest. A histological and histomorphometric analysis in rats. Int. J. Oral Maxillofac. Surg. 2011, 40, 533–542. [Google Scholar] [CrossRef] [PubMed]
- Ghanaati, S.; Kirkpatrick, C.J.; Kubesch, A.; Lorenz, J.; Sader, R.A.; Udeabor, S.E.; Barbeck, M.; Choukroun, J. Induction of multinucleated giant cells in response to small sized bovine bone substitute (Bio-OssTM) results in an enhanced early implantation bed vascularization. Ann. Maxillofac. Surg. 2014, 4, 150–157. [Google Scholar] [CrossRef] [Green Version]
- Kuchler, U.; Dos Santos, G.M.; Heimel, P.; Stähli, A.; Strauss, F.J.; Tangl, S.; Gruber, R. DBBM shows no signs of resorption under inflammatory conditions. An experimental study in the mouse calvaria. Clin. Oral Implant. Res. 2019, 31, 10–17. [Google Scholar] [CrossRef] [Green Version]
- Kassem, A.; Henning, P.; Lundberg, P.; Souza, P.P.; Lindholm, C.; Lerner, U.H. Porphyromonas gingivalis Stimulates Bone Resorption by Enhancing RANKL (Receptor Activator of NF-kappaB Ligand) through Activation of Toll-like Receptor 2 in Osteoblasts. J Biol Chem 2015, 33, 20147–20158. [Google Scholar] [CrossRef] [Green Version]
- Von Knoch, M.; Jewison, D.; Sibonga, J.; Turner, R.; Morrey, B.; Löer, F.; Berry, D.; Scully, S. Decrease in particle-induced osteolysis in obese (ob/ob) mice. Biomaterials 2004, 25, 4675–4681. [Google Scholar] [CrossRef]
- Jensen, S.S.; Aaboe, M.; Janner, S.F.M.; Saulacic, N.; Bornstein, M.M.; Bosshardt, D.D.; Buser, D. Influence of Particle Size of Deproteinized Bovine Bone Mineral on New Bone Formation and Implant Stability after Simultaneous Sinus Floor Elevation: A Histomorphometric Study in Minipigs. Clin. Implant. Dent. Relat. Res. 2013, 17, 274–285. [Google Scholar] [CrossRef] [Green Version]
- Fuegl, A.; Tangl, S.; Keibl, C.; Watzek, G.; Redl, H.; Gruber, R. The impact of ovariectomy and hyperglycemia on graft consolidation in rat calvaria. Clin. Oral Implant. Res. 2011, 22, 524–529. [Google Scholar] [CrossRef] [PubMed]
- Benic, G.I.; Eisner, B.M.; Jung, R.E.; Basler, T.; Schneider, D.; Hämmerle, C.H.F. Hard tissue changes after guided bone regeneration of peri-implant defects comparing block versus particulate bone substitutes: 6-month results of a randomized controlled clinical trial. Clin. Oral Implant. Res. 2019, 30, 1016–1026. [Google Scholar] [CrossRef] [PubMed]
- Mir-Mari, J.; Benic, G.I.; Valmaseda-Castellón, E.; Hämmerle, C.H.F.; Jung, R.E. Influence of wound closure on the volume stability of particulate and non-particulate GBR materials: An in vitro cone-beam computed tomographic examination. Part II. Clin. Oral Implant. Res. 2016, 28, 631–639. [Google Scholar] [CrossRef] [Green Version]
- Xie, L.; Yu, H.; Deng, Y.; Yang, W.; Liao, L.; Long, Q. Preparation, characterization and in vitro dissolution behavior of porous biphasic alpha/beta-tricalcium phosphate bioceramics. Mater. Sci. Eng. C Mater. Biol. Appl. 2016, 59, 1007–1015. [Google Scholar] [CrossRef] [PubMed]
- Hothorn, T.; Bühlmann, P.; Dudoit, S.; Molinaro, A.; Van Der Laan, M.J. Survival ensembles. Biostatics 2005, 7, 355–373. [Google Scholar] [CrossRef]
- Westfall, P.H.; Troendle, J.F. Multiple Testing with Minimal Assumptions. Biom. J. 2008, 50, 745–755. [Google Scholar] [CrossRef] [Green Version]
- Ohba, S.; Sumita, Y.; Umebayashi, M.; Yoshimura, H.; Yoshida, H.; Matsuda, S.; Kimura, H.; Asahina, I.; Sano, K.; Information, P.E.K.F.C. Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma. Arch. Oral Biol. 2016, 61, 23–27. [Google Scholar] [CrossRef]
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Kuchler, U.; Heimel, P.; Stähli, A.; Strauss, F.J.; Luza, B.; Gruber, R. Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice. Materials 2020, 13, 4748. https://doi.org/10.3390/ma13214748
Kuchler U, Heimel P, Stähli A, Strauss FJ, Luza B, Gruber R. Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice. Materials. 2020; 13(21):4748. https://doi.org/10.3390/ma13214748
Chicago/Turabian StyleKuchler, Ulrike, Patrick Heimel, Alexandra Stähli, Franz Josef Strauss, Bernadette Luza, and Reinhard Gruber. 2020. "Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice" Materials 13, no. 21: 4748. https://doi.org/10.3390/ma13214748