Covalent Immobilization of Organic Photosensitizers on the Glass Surface: Toward the Formation of the Light-Activated Antimicrobial Nanocoating
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Formation and Characterization of the Photoactive Layer
2.2.1. Glass Substrate Pretreatment
2.2.2. Photoactive Monolayer Formation
2.2.3. X-ray Photoelectron Spectroscopy
2.2.4. Raman Spectroscopy
2.3. Photogeneration of Reactive Oxygen Species (ROS)
2.4. Microbiological Analysis
2.4.1. Bacterial Strain and Culture Conditions
2.4.2. Bacterial Cell Staining and Imaging
3. Results and Discussion
3.1. Chemical Grafting and Spectroscopic Characterization of the Photoactive Mixed Monolayer (MIX_TC_APTES@glass)
3.1.1. Chemical Grafting and XPS Spectroscopy
3.1.2. Raman Spectroscopy
3.2. Reactive Oxygen Species (ROS) Photogeneration by the Chemically Grafted Mixed Monolayer
3.3. Antimicrobial Properties of the Chemically Grafted Mixed Monolayer
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Nyga, A.; Czerwińska-Główka, D.; Krzywiecki, M.; Przystaś, W.; Zabłocka-Godlewska, E.; Student, S.; Kwoka, M.; Data, P.; Blacha-Grzechnik, A. Covalent Immobilization of Organic Photosensitizers on the Glass Surface: Toward the Formation of the Light-Activated Antimicrobial Nanocoating. Materials 2021, 14, 3093. https://doi.org/10.3390/ma14113093
Nyga A, Czerwińska-Główka D, Krzywiecki M, Przystaś W, Zabłocka-Godlewska E, Student S, Kwoka M, Data P, Blacha-Grzechnik A. Covalent Immobilization of Organic Photosensitizers on the Glass Surface: Toward the Formation of the Light-Activated Antimicrobial Nanocoating. Materials. 2021; 14(11):3093. https://doi.org/10.3390/ma14113093
Chicago/Turabian StyleNyga, Aleksandra, Dominika Czerwińska-Główka, Maciej Krzywiecki, Wioletta Przystaś, Ewa Zabłocka-Godlewska, Sebastian Student, Monika Kwoka, Przemysław Data, and Agata Blacha-Grzechnik. 2021. "Covalent Immobilization of Organic Photosensitizers on the Glass Surface: Toward the Formation of the Light-Activated Antimicrobial Nanocoating" Materials 14, no. 11: 3093. https://doi.org/10.3390/ma14113093